Elucidation of the Na and K transport system conferring salinity tolerance in plant cells

• Project/Area Number: 22380056
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Tohoku University
• Principal Investigator: UOZUMI Nobuyuki 東北大学, 工学(系)研究科(研究院), 教授 (40223515)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 輸送体 / カリウム / ナトリウム / 遺伝子発現 / 膜タンパク質 / 耐塩性 / 浸透圧 / AtHKT1 / K輸送体 / シロイヌナズナ / イオンバランス / AtNHX1 / プロモーター / Na循環系 / 概日性リズム発現 / ルシフェラーゼ遺伝子 / Na輸送体 / AtKUP1 / AtHB

Research Abstract

Drought stress and accumulation of salt lead to increase the osmolarity of the environmental space. This gave the dehydration of plant cells, which ends up to jeopardize the plant life. The water movement across the biological membrane is affected by osmolarity and therefore the concentrations of cation, sodium ion and potassium ion are crucial for them. The intracellular concentrations of these cation play an important role in ion homeostasis in plant cells. In this study, membrane transport system for sodium ion and potassium ion has been analyzed and their structure and function has been investigated. The increase of the resistance against the environmental stress in plant cells has been studied through the elucidation of their sodium and potassium transport system, which is closely involved in understanding the molecular mechanism of osmolarity homeostasis.

Research Products

• [Journal Article] Thylakoid potassium channel is required for efficient photosynthesis in cyanobacteria (2012)
  • Author(s): Checchetto, V., Segalla, A., Allorent, G., Rocca, N. L., Leanza, L., Giacometti, G.M., Uozumi, N., Finazzi, G., Bergantinoa,E., and Szabo, I
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Issue: 27 Pages: 11043-11048
  • DOI: 10.1073/pnas.1205960109
  • Peer Reviewed
• [Journal Article] Structure-Function Correlates in Plant Ion Channels In Egelman, E. (ed.) (2012)
  • Author(s): Uozumi, N. and Deryer, I
  • Journal Title: Comprehensive Biophysics Volume 6 - Channel Proteins Elsevier BV Amsterdam Volume: 6 Pages: 234-245
  • DOI: 10.1016/b978-0-12-374920-8.00628-7
  • ISBN: 9780080957180
  • Peer Reviewed
• [Journal Article] Regulatory mechanism of plant nyctinastic movement (2012)
  • Author(s): Ishimaru, Y., Hamamoto, S., Uozumi N., and Ueda, M
  • Journal Title: An ion channel-related plant behavior Plant Electrophysiology(In Volkov, A. G. (ed.) Spring-Verlag, Berlin Heidelberg) Volume: - Pages: 125-142
  • DOI: 10.1007/978-3-642-29110-4_5
  • ISBN: 9783642291098, 9783642291104
  • Peer Reviewed
• [Journal Article] Potassium and sodium transporters : Improving salinity tolerance in plants In Tuteja, N., Gill, S.S., Tiburcio, A. F., Tuteja, R. (ed.) (2012)
  • Author(s): Yamaguchi1, T., Uozumi, N., and Horie, T
  • Journal Title: Improving Crop Resistance to Abiotic Stress Volume: 23 Pages: 523-542
  • DOI: 10.1002/9783527632930.ch23
  • Peer Reviewed
• [Journal Article] A simple fed-batch method for transcription and insect cell-free translation (2011)
  • Author(s): Sato,Y., Aizawa, K., Ezure, T., Ando, E., Uozumi, N.
  • Journal Title: J. Bioscience and Bioeng. Volume: 114 Issue: 6 Pages: 677-679
  • DOI: 10.1016/j.jbiosc.2012.06.015
  • NAID: 110009578165
  • Peer Reviewed
• [Journal Article] Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp (2011)
  • Author(s): Akai, M., Onai, K., Kusano, M., Sato, M., Redestig, H., Toyooka, K., Morishita, M., Miyake, H., Hazama, A., Checchetto, V., Szabo, I., Matsuoka, K., Saito, K., Yasui, M., Ishiura, M., and Uozumi, N
  • Journal Title: PCC 6803. J. Biol Volume: 286 Issue: 28 Pages: 25224-25235
  • DOI: 10.1074/jbc.m111.236380
  • Peer Reviewed
• [Journal Article] Pollen tubes lacking a pair of K+ transporters fail to target ovules in Arabidopsis (2011)
  • Author(s): Lu, Y., Chanroj, S., Zulkifli, L., Johnson, MR., Uozumi, N., Cheung, A., and Sze, H
  • Journal Title: Plant Cell Volume: 23 Issue: 1 Pages: 81-93
  • DOI: 10.1105/tpc.110.080499
  • Peer Reviewed
• [Journal Article] Potassium channels in plant cells (2011)
  • Author(s): Dreyer, I. and Uozumi, N
  • Journal Title: FEBS Journal Volume: 278 Pages: 4293-4303
  • Peer Reviewed
• [Journal Article] Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting J (2011)
  • Author(s): Chanroj, S., Lu, Y., Padmanaban, S., Nanatani, K., Uozumi, N., Rao, R., and Sze, H
  • Journal Title: Biol Volume: 286 Issue: 39 Pages: 3393-3393
  • DOI: 10.1074/jbc.m111.252650
  • Peer Reviewed
• [Journal Article] Plasma membrane aquaporin AqpZ protein is essential for glucose meta bolism during photomixotrophic growth of Synechocystis sp.PCC 6803 (2011)
  • Author(s): Akai, et al
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 25224-25235
  • Peer Reviewed
• [Journal Article] The KtrA and KtrE subunits are required for Na^+ dependent K^+ uptake by KtrB across the plasma membrane in Synechocystis sp (2010)
  • Author(s): Zulkifli, L., Akai, M., Yoshikawa, A., Shimojima, M., Ohta, H., Guy HR., and Uozumi, N
  • Journal Title: PCC 6803. J. Bacteriol Volume: 192 Issue: 19 Pages: 5063-5070
  • DOI: 10.1128/jb.00569-10
  • Peer Reviewed
• [Journal Article] Roles of tandem-pore K^+ channels in plants - a puzzle still to be solved (2010)
  • Author(s): Voelker, C., Gomez-Porras, J. L., Becker, D., Hamamoto, S., Uozumi, N., Gambale, F., Mueller-Roeber, B., Czempinski, K., and Dreyer, I
  • Journal Title: Plant Biology Volume: 12 Issue: s1 Pages: 56-63
  • DOI: 10.1111/j.1438-8677.2010.00353.x
  • Peer Reviewed
• [Journal Article] Roles of tandem-pore K+ channels in plants -a puzzle still to be solved (2010)
  • Author(s): Voelker, et al
  • Journal Title: Plant Biology Volume: 12 Pages: 56-63
  • Peer Reviewed
• [Journal Article] The KtrA and KtrE subunits are required for Na+ dependent K+ uptake by KtrB across the plasma membrane in Synechocystis sp.PCC 6803 (2010)
  • Author(s): Zulkifli, et al
  • Journal Title: J.Bacteriol. Volume: 192 Pages: 5063-5070
  • Peer Reviewed
• [Presentation] Membrane transport system conferring the salinity tolerance to bacteria and plant cells (2012)
  • Author(s): Uozumi, N., Nanatani K., and Hamamoto, S
  • Organizer: 6th Japan-Finland Biotechnology Symposium 2012
  • Place of Presentation: Sendai
  • Year and Date: 2012-06-05
• [Presentation] Shaker型K+チャネルファミリーに保存されている活性制御部位の解析 (2012)
  • Author(s): 中山浩太、松本尚樹、浜本晋、魚住信之
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-25
• [Presentation] Arabidopsis thaliana Shakerチャネルの昆虫細胞系構築とリン酸化部位の検討 (2012)
  • Author(s): 南雲隆太,七谷圭,山形敦史,深井周也,魚住信之
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-16
• [Presentation] Synechocystis sp. PCC 6803の浸透圧調節に関与するKdp系Kトランスポーターの機能解析 (2012)
  • Author(s): 七谷圭、四十九俊彰、高野洋佑、山崎智子、Lalu Zulkifli、赤井政郎、飯塚龍、松本秀之、丸山央峰、新井史人、魚住信之
  • Organizer: 創立90周年記念第64回日本生物工学会大会
  • Place of Presentation: 神戸市
• [Presentation] 植物K流入系チャネルAKT1のリン酸化活性制御 (2012)
  • Author(s): 浜本晋,中山浩太,松本尚樹,佐藤裕樹,七谷圭,魚住信之
  • Organizer: 創立90周年記念第64回日本生物工学会大会
  • Place of Presentation: 神戸市
• [Presentation] Functional characterization of K transporters in Synechocystis sp. PCC 6803 (2012)
  • Author(s): Nanatani, K., Shijuku, Y., Takano, Y., Yamazaki, T., Zulkifli, L., Akai,M., Iitsuka, R., Matsumoto, H., Maruyama, H., Arai F., and Uozumi, N
  • Organizer: The IUBMB & FEBS 2012 Congress
  • Place of Presentation: Seville, Spain
• [Presentation] Characterization of YVC1 Channel in Yeast Vacuolar Membrane (2012)
  • Author(s): Hamamoto, S., Mori, Y., Yabe, I., Uozumi, N
  • Organizer: 2012 International Ion Channel Conference
  • Place of Presentation: Jeju, Korea
• [Presentation] Functional characterization of K transporters in Synechocystis sp. PCC 6803. (2012)
  • Author(s): Nanatani, K., Shijuku, Y., Takano, Y., Yamazaki, T., Zulkifli, L., Akai,M., Iitsuka, R., Matsumoto, H., Maruyama, H., Arai F., and Uozumi, N.
  • Organizer: The IUBMB & FEBS 2012 Congress
  • Place of Presentation: Seville, Spain
• [Presentation] Characterization of YVC1 Channel in Yeast Vacuolar Membrane (2012)
  • Author(s): Shin Hamamoto, Yasuo Mori, Isamu Yabe, Nobuyuki Uozumi
  • Organizer: 2012 International Ion Channel Conference
  • Place of Presentation: Jeju，Korea
• [Presentation] Synechocystis sp. PCC 6803の浸透圧調節に関与するKdp系Kトランスポーターの機能解析 (2012)
  • Author(s): 七谷圭、四十九俊彰、高野洋佑、山崎智子、Lalu Zulkifli、赤井政郎、飯塚龍、松本秀之、丸山央峰、新井史人、魚住信之
  • Organizer: 創立90周年記念 第64回日本生物工学会大会
  • Place of Presentation: 神戸市
• [Presentation] 植物K流入系チャネルAKT1のリン酸化活性制御 (2012)
  • Author(s): 浜本晋 中山浩太 松本尚樹 佐藤裕樹 七谷圭 魚住信之
  • Organizer: 創立90周年記念 第64回日本生物工学会大会
  • Place of Presentation: 神戸市
• [Presentation] 植物のセシウム吸収力について (2011)
  • Author(s): 魚住信之
  • Organizer: 西郷村・生物工学シンポジウム
  • Place of Presentation: 福島県西郷村
  • Year and Date: 2011-11-17
• [Presentation] 膜駆動系を構成する植物・ラン藻の輸送体 (2011)
  • Author(s): 魚住信之
  • Organizer: 第5回ハイスループットスクリーニングシステムと次世代DNAシーケンサーの相乗的応用
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-09-14
• [Presentation] 植物イオンチャネル・トランスポーターの機能制御と分子基盤 (2011)
  • Author(s): 魚住信之
  • Organizer: 第6回トランスポーター研究会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-06-11
• [Presentation] 植物イオンチャネル・トランスポーターの機能制御と分子基盤 (2011)
  • Author(s): 魚住信之
  • Organizer: 第６回トランスポーター研究会年会
  • Place of Presentation: 仙台市
• [Presentation] 巨大化酵母パッチクランプ法を用いた酵母陽イオンチャネルYVC1の輸送特性 (2010)
  • Author(s): 浜本晋、森泰生、矢部勇、魚住信之
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-10-29
• [Presentation] 高・低浸透圧ストレス適応に寄与するラン藻Synechocystis PCC6803のKdp・Msc膜輸送系の解析 (2010)
  • Author(s): 七谷圭、高野洋佑、鈴木石根、四十九俊彰、魚住信之
  • Organizer: 日本農芸化学会東北支部・北海道支部合同支部大会(東北支部第145回大会)
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-28
• [Presentation] KAT1 is differentially phosphorylated by SnRK2.6 and calcium-dependent kinase, which reduces its transport activity (2010)
  • Author(s): Uozumi, et al
  • Organizer: International Workshop on Plant Membrane Biology
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2010-09-23
• [Presentation] KAT1 is differentially phosphorylated by SnRK2.6 and calcium-dependent kinase, which reduces its transport activity (2010)
  • Author(s): Uozumi, N., Sato, A, Sato, Y, Fukao, Y, Fujiwara, M, Umezawa ,T, Shinozaki, K, Hibi, T, Taniguchi, M, Miyake, M, Dreyer, I, Gambale, F, Goto, D
  • Organizer: XV International Workshop on Plant Membrane Biology
  • Place of Presentation: Adelaide, Australia
• [Presentation] Membrane transport system conferring the salinity tolerance to bacteria and plant cells.
  • Author(s): Uozumi, N., Nanatani K., and Hamamoto, S.
  • Organizer: 6th Japan-Finland Biotechnology Symposium
  • Place of Presentation: Ｓｅｎｄａｉ