Identification and utilization of genes for carbon-concentratingmechanism and hydrogen production in microalga by digital expression analyses.
| Project/Area Number |
22380059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
FUKUZAWA Hideya 京都大学, 大学院・生命科学研究科, 教授 (30183924)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANO Takashi 京都大学, 大学院・生命科学研究科, 助教 (70570167)
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| Research Collaborator |
KAJIKAWA Masataka 京都大学, 大学院・生命科学研究科, 助教 (40594437)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 二酸化炭素 / 緑藻 / エネルギー / 光合成 / トランスクリプトーム / 微細藻類 / 緑藻クラミドモナス / バイオ燃料 / 炭酸固定 / 代謝工学 / 形質転換 / クラミドモナス / 水素発生 / RNA-seq / 二酸化炭素費 / 水素燃料 / 無機炭素濃縮機構 |
|---|
| Research Abstract |
Algal photosynthesis, which is base for production of bio-materials, is supported by carbon-concentrating mechanism (CCM). To identify CCM-related genes, RNA-seq analyses using different RNA samples prepared from cells cultured in various stress conditions including low-CO2 or nitrogen starved conditions. Using RNA-seq data and genomic sequence data, we have developed a new expression database for green alga, Chlamydomonas reinhardtii. Genes which are induced in low-CO2 stress conditions, nitrogen-starved conditions, and anaerobic conditions were identified by comparing the RNA expression profiles. Several CCM-related genes were artificially overexpressed in transgenic Chlamydomonas cells and the photosynthetic characteristics were show to be significantly improved than wild type cells.
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Report
(4 results)
Research Products
(61 results)
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[Presentation] Genomewide response to CO_2 deficiency in Chlamydomonas reinhardtii revealed by RNA-seq analysis2013
Author(s)
Yamano T, Taniguchi T, Kubo T, Yanase M, Suzuki Y, Sugano S, Itoh T, Fujiyama A, Kohara Y, Toyoda A, Shin-i T, Fukuzawa H
Organizer
International Symposium on Genome Science-Expanding Frontiers of Genome Science
Place of Presentation
University of Tokyo, Tokyo
Year and Date
2013-01-09
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[Presentation] Genome-wide response to CO_2 deficiency in Chlamydomonas reinhardtii revealed by RNA-seq analysis2012
Author(s)
Yamano T, Taniguchi T, Kubo T, Yanase M, Suzuki Y, Sugano S, Itoh T, Fujiyama A, Kohara Y, Toyoda A, Shin-i T, Fukuzawa H
Organizer
15th International Conference on the Cell and Molecular Biology of Chlamydomonas
Place of Presentation
Potsdam, Germany
Year and Date
2012-06-05
Related Report
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[Presentation] Genome-wide response to CO_2 deficiency in Chlamydomonas reinhardtiirevealed by RNA-seq analysis2012
Author(s)
Yamano T, Kubo T, Taniguchi T, Yanase M, Suzuki Y, Sugano S, Itoh T, Fujiyama A, Kohara Y, Toyoda A, Shin-i T, Kuroki Y, Fukuzawa H
Organizer
The 1st International Symposium on Plant Environmental Sensing
Place of Presentation
東大寺総合文化センター
Year and Date
2012-03-19
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[Presentation] Genome-wide response to CO2 deficiency in Chlamydomonas reinhardtii revealed by RNA-seq analysis2012
Author(s)
Takashi Yamano, Takeaki Kubo, Takeaki Taniguchi, Mari Yanase, Yutaka Suzuki, Sumio Sugano, Takehiko Itoh, Asao Fujiyama, Yuji Kohara, Atsushi Toyoda, Tadasu Shin-i, Yoko Kuroki, Hideya Fukuzawa
Organizer
The 1st International Symposium on Plant Environmental Sensing
Place of Presentation
東大寺総合文化センター
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