| Project/Area Number |
22380061
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Nara Institute of Science and Technology |
|---|
Principal Investigator |
TAKAGI Hiroshi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (50275088)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Nobuyuki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (10273848)
OHTSU Iwao 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (60395655)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SHINOZAKI Kazuo 独立行政法人理化学研究所, 植物科学研究センター, センター長 (20124216)
HIBI Takao 福井県立大学, 生物資源学部, 教授 (00285181)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
|---|
| Keywords | 酵母 / アセチル化酵素 / 酸化ストレス / 活性酸素種 / プロリン代謝 / アミノ酸代謝 |
|---|
| Research Abstract |
We found that the yeast N-acetyltransferase Mpr1 is a novel anti-oxidative enzyme that acetylates the proline catabolism intermediate, which induces the generation of superoxide anions by inhibiting the mitochondrial respiration, and that accelerates arginine synthesis, leading to the arginine-dependent NO production, which confers oxidative stress tolerance on yeast. Tah18 was first identified as the yeast protein involved in NO synthesis. We also analyzed the X-ray crystal structure and the Mpr1 mutants, indicating that Mpr1 has a unique catalytic reaction mechanism and that the catalytic residues are crucial for oxidative stress tolerance of yeast.
|
