Mechanisms of gene regulation mediated by a novel nuclear mRNP

• Project/Area Number: 22380063
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: IMAI Ryozo 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター・寒地作物研究領域, 上席研究員 (90291913)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: mRNA / ポリA結合タンパク質 / シロイヌナズナ / ストレス耐性 / mRNP / 植物 / ストレス / 発現制御 / RNA制御

Research Abstract

Nuclear polyA-binding protein, PABN1, was characterized in this study. PABN1 interacted with the cold shock domain protein, AtCSP3, within nuclear speckles and regulates freezing, drought and salt stress tolerance. The mRNP may also contain SPL4, a regulator of flowering time.

Research Products

• [Journal Article] Interactome analysis reveals versatile functions of Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 in RNA processing within the nucleus and cytoplasm (2013)
  • Author(s): Kim, M.-H., Sonoda, Y., Sasaki, K., Kaminaka, H., Imai, R.
  • Journal Title: Cell Stress and Chaperones Volume: 18(4) Issue: 4 Pages: 517-525
  • DOI: 10.1007/s12192-012-0398-3
  • Peer Reviewed
• [Journal Article] Arabidopsis COLD SHOCK DOMAIN PROTEIN 2 is a negative regulator of cold acclimation (2013)
  • Author(s): Sasaki, K., Kim, M.-H., Imai, R.
  • Journal Title: New Phytologist Volume: 198(1) Issue: 1 Pages: 95-102
  • DOI: 10.1111/nph.12118
  • Peer Reviewed
• [Journal Article] Pleiotropic roles of cold shock domain proteins in plants (2011)
  • Author(s): Sasaki K and Imai R
  • Journal Title: Frontier in Plant Sci Volume: 2 Pages: 116-116
• [Journal Article] Pleiotropic roles of cold shock domain proteins in plants (2011)
  • Author(s): Kentaro Sasaki
  • Journal Title: Frontiers in Plant Science Volume: 2 Pages: 116-116
  • DOI: 10.3389/fpls.2011.00116
  • Peer Reviewed
• [Presentation] COLD SHOCK DOMAIN PROTEIN 3 regulates salt and drought stress tolerance in Arabidopsis (2013)
  • Author(s): Shunya Sato
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] Functional analysis of nuclear poly (A) binding protein 1 (PABN1) that interacts with CSP3 in Arabidopsis (2012)
  • Author(s): Kim, M.H., Sonoda, Y., Sasaki, K., Imai, R.
  • Organizer: Plant and Microbe Adaptations to Cold 2012
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2012-06-25
• [Presentation] Functional analysis of nuclear poly (A) binding protein 1 (PABN1) that interacts with CSP3 in Arabidopsis (2012)
  • Author(s): Myung Hee Kim
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] Cold Shock Domain proteins regulate stress response and development in Arabidopsis (2012)
  • Author(s): Ryozo Imai
  • Organizer: Plant and Microbe Adaptations to Cold 2012
  • Place of Presentation: Hokkaido University
  • Invited
• [Presentation] Functional analysis of nuclear poly (A) binding protein 1 (PABN1) that interacts with CSP3 in Arabidopsis (2012)
  • Author(s): Myung Hee Kim
  • Organizer: Plant and Microbe Adaptations to Cold 2012
  • Place of Presentation: Hokkaido University
• [Presentation] Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3) functions in multiple abiotic stress tolerance in Arabidopsis (2012)
  • Author(s): Shunya Sato
  • Organizer: Plant and Microbe Adaptations to Cold 2012
  • Place of Presentation: Hokkaido University
• [Presentation] Arabidopsis COLD SHOCK DOMAIN PROTEIN 2 is a negative regulator of cold acclimation and seed germination (2012)
  • Author(s): Kentaro Sasaki
  • Organizer: Plant and Microbe Adaptations to Cold 2012
  • Place of Presentation: Hokkaido University
• [Presentation] The Arabidopsis Cold Shock Domain protein 3 (AtCSP3) interacts with RNA processing proteins in the nucleus and cytoplasm (2011)
  • Author(s): Myung Hee Kim
  • Organizer: International Plant RNA Workshop 2011
  • Place of Presentation: 横浜
  • Year and Date: 2011-06-20
• [Presentation] RNA chaperones regulate stress responses and development in plants (2011)
  • Author(s): Imai, R., Kim, M.H., Sasaki, K., Tabata, D.
  • Organizer: (Invited talk) International Plant RNA Workshop
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2011-06-19
• [Book] In Plant and Microbe Adaptations to Cold in a Changing World. eds. Imai, R., Yoshida, M., Matsumoto, N., Springer, New York, USA. in press. (2013)
  • Author(s): Imai, R., Kim, M.H., Sasaki, K., Sato, S..,Sonoda, Y.
  • Publisher: Cold shock domain proteins in Arabidopsis: functions in stress tolerance and development
• [Book] Plant and Microbe Adaptations to Cold in a Changing World (2013)
  • Author(s): Ryozo Imai, Myung Hee Kim, Kentaro Sasaki, Shunya Sato and Yutaka Sonoda
  • Total Pages: 300
  • Publisher: Springer, N.Y.
• [Patent(Industrial Property Rights)] 高い種子収量を有する環境ストレス耐性植物及びその作製方法 (2013)
  • Inventor(s): 今井 亮三, 金 明姫, 柳楽 洋三, 田岡 直明
  • Industrial Property Rights Holder: (国研)農研機構, (株)カネカ
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-03-07
  • Acquisition Date: 2017-09-08
• [Patent(Industrial Property Rights)] 高い種子収量性を有する環境ストレス 耐性植物及びその作製方法 (2012)
  • Inventor(s): 今井亮三,金明姫,柳楽洋三,田岡直明
  • Industrial Property Rights Holder: 独)農業・食品産業技術総合研究機 構,株)カネカ
  • Industrial Property Number: 2012-052018
  • Filing Date: 2012-03-08
• [Patent(Industrial Property Rights)] 高い種子収量性を有する環境ストレス耐性植物及びその作成方法 (2012)
  • Inventor(s): 今井亮三, 金明姫, 柳楽洋三, 田岡直明
  • Industrial Property Rights Holder: 独立行政法人農業・食品産業技術総合研究機構, 株式会社カネカ
  • Industrial Property Number: 2012-052018
  • Filing Date: 2012-03-08