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Enhancement of dietary peptide-induced GLP-1 secretion by indigestible saccharides

Research Project

Project/Area Number 22380070
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionHokkaido University

Principal Investigator

HARA Hiroshi  北海道大学, (連合)農学研究科(研究院), 教授 (70198894)

Co-Investigator(Kenkyū-buntansha) HIRA Tohru  北海道大学, 大学院農学研究院, 講師 (10396301)
Project Period (FY) 2010-04-01 – 2013-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
KeywordsGLP-1 / 消化抵抗性糖質 / 食品ペプチド / 消化管ホルモン / 難消化性糖質 / 難消化性ペプチド / インクレチン / 低消化性糖質
Research Abstract

GLP-1 is one of gastrointestinal hormones, and it has potent anti-obese and anti-diabetic effects. In the present project, we investigated whether indigestible peptides or indigestible saccharides are effective to increase GLP-1 secretion in a cell culture model and animal models. We also examined combined effects of indigestible saccharides and a dietary peptide.
We demonstrated that oral administration of a dietary peptide (corn peptide) potently stimulated GLP-1 secretion and it resulted in reduced glycemic response in normal and diabetic rats.
Luminal and oral administration of an indigestible saccharide (resistant maltodextrin, RMD) stimulated GLP-1 secretion in rats, and continuous ingestion of RMD also enhanced basal GLP-1 secretion. Furthermore, rats continuously having RMD showed increased GLP-1 secretory response to oral administration the corn peptide. These results revealed potential and combined effects of indigestible saccharides and dietary peptides in vivo.

Report

(4 results)
  • 2013 Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (51 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (16 results) (of which Peer Reviewed: 11 results) Presentation (34 results) (of which Invited: 6 results) Remarks (1 results)

  • [Journal Article] A nondigestible saccharide, fructooligosaccharide, increases the promotive effect of a flavonoid,α-glucosyl-isoquercitrin, on glucagon-like peptide 1 (GLP-1) secretion in rat intestine and enteroendocrine cells2014

    • Author(s)
      Phuwamongkolwiwat P, Hira T, Hara H
    • Journal Title

      Mol Nutr Food Res

      Volume: (in press) Issue: 7 Pages: 1581-1584

    • DOI

      10.1002/mnfr.201300871

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unsaturated aldehydes induce CCK secretion via TRPA1 in STC-1 cells2014

    • Author(s)
      Nakajima S, Hira T, Yahagi A, Nishiyama C, Yamashita T, Imagi J, Hara H
    • Journal Title

      Mol Nutr Food Res

      Volume: 58(5) Issue: 5 Pages: 1042-51

    • DOI

      10.1002/mnfr.201300412

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fructooligosaccharide augments benefits of quercetin-3-O-β-glucoside on insulin sensitivity and plasma total cholesterol with promotion of flavonoid absorption in sucrose-fed rats2014

    • Author(s)
      Phuwamongkolwiwat P, Suzuki T, Hira T, Hara H
    • Journal Title

      Eur J Nutr. Mar

      Volume: 53(2) Issue: 2 Pages: 457-68

    • DOI

      10.1007/s00394-013-0546-2

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A nondigestible saccharide, fructooligosaccharide, increases the promotive effect of a flavonoid, alpha-glucosyl-isoquercitrin, on glucagon-like peptide 1 (GLP-1) secretion in rat intestine and enteroendocrine cells2014

    • Author(s)
      Phuwamongkolwiwat P, Hira T, Hara H.
    • Journal Title

      Molecular Nutrition and Food Research

      Volume: 印刷中

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oral administration of corn Zein hydrolysate stimulates GLP-1 and GIP secretion and improves glucose tolerance in male normal rats and Goto-Kakizaki rats2013

    • Author(s)
      Higuchi N, Hira T, Yamada N, Hara H
    • Journal Title

      Endocrinology

      Volume: 154(9) Issue: 9 Pages: 3089-98

    • DOI

      10.1210/en.2012-2275

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 腸管での栄養素認識と消化管ホルモン分泌に関する研究(平成24年度日本栄養・食糧学会奨励賞受賞)2012

    • Author(s)
      比良徹
    • Journal Title

      日本栄養・食糧学会誌

      Volume: 65(5) Pages: 215-220

    • NAID

      130002129275

    • Related Report
      2013 Final Research Report
  • [Journal Article] Potato extract (Potein) suppresses food intake in rats through inhibition of luminal trypsin activity and direct stimulation of cholecystokinin secretion from enteroendocrine cells.2011

    • Author(s)
      Nakajima S
    • Journal Title

      J Agric Food Chem.

      Volume: 59 Issue: 17 Pages: 9491-9496

    • DOI

      10.1021/jf200988f

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acute effect of soybean beta-conglycinin hydrolysate ingestion on appetite sensations in healthy humans2011

    • Author(s)
      Hira T, Mori N, Nakamori T, Furuta H, Asano K, Chiba H, Hara H
    • Journal Title

      Appetite

      Volume: 57(3) Issue: 3 Pages: 765-8

    • DOI

      10.1016/j.appet.2011.07.014

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Soybeanβ-Conglycinin Bromelain Hydrolysate Stimulates Cholecystokinin Secretion by Enteroendocrine STC-1 Cells to Suppress the Appetite of Rats under Meal-Feeding Conditions2011

    • Author(s)
      Sufian KN, Hira T, Nakamori T, Furuta H, Asano K, Hara H
    • Journal Title

      Bioscience, Biotechnology and Biochemistry

      Volume: 75(5) Pages: 845-53

    • NAID

      10028272318

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] GLP-1 secretion in response to oral and luminal palatinose (isomaltulose) in rats2011

    • Author(s)
      Hira T, Muramatsu M, Okuno M, Hara H
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 57(1) Pages: 30-25

    • NAID

      10028106023

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 消化管ホルモンを介して食欲をコントロールする食品由来ペプチド2011

    • Author(s)
      原博、中島進吾、比良徹
    • Journal Title

      臨床化学(特集)(日本臨床化学会)

      Volume: 40(2) Pages: 119-12

    • Related Report
      2013 Final Research Report
  • [Journal Article] インクレチン分泌を刺激する食品ペプチド、アミノ酸?消化管内分泌細胞による栄養素認識、Nutirient sensingの解明と応用に期待2011

    • Author(s)
      比良徹
    • Journal Title

      化学と生物、今日の話題(日本農芸化学会)

      Volume: 48(No. 1) Pages: 11-13

    • Related Report
      2013 Final Research Report
  • [Journal Article] Soybean .BETA.-Conglycinin Bromelain Hydrolysate Stimulates Cholecystokinin Secretion by Enteroendocrine STC-1 Cells to Suppress the Appetite of Rats under Meal-Feeding Conditions2011

    • Author(s)
      Bin Sufian, Md. Kaosar Niaz; Hira, Tohru; Nakamori, Toshihiro; Furuta, Hitoshi; Asano, Kozo; Hara, Hiroshi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 5 Pages: 848-853

    • DOI

      10.1271/bbb.100765

    • NAID

      10028272318

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GLP-1 Secretion in Response to Oral and Luminal Palatinose (Isomaltulose) in Rats2011

    • Author(s)
      Hira T, Muramatsu M, Okuno M, Hara H
    • Journal Title

      J Nutr Sci Vitaminol

      Volume: 57(1) Pages: 30-35

    • NAID

      10028106023

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] インクレチン分泌を刺激する食品ペプチド、アミノ酸~消化管内分泌細胞による栄養素認識、Nutirient sensingの解明と応用に期待2011

    • Author(s)
      比良徹
    • Journal Title

      化学と生物(今日の話題)

      Volume: 48(1) Pages: 11-13

    • Related Report
      2010 Annual Research Report
  • [Journal Article] ラットにおけるトウモロコシZein加水分解物によるGLP-1分泌を介した血糖上昇抑制2010

    • Author(s)
      比良徹、持田泰佑、原博
    • Journal Title

      アミノ酸研究

      Volume: 4(2) Pages: 163-66

    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] Effects of resistant maltodextrin and fructooligosaccharides on fasting GLP-1 levels and glucose tolerance in rats2014

    • Author(s)
      Tohru Hira, Asuka Ikee, Yuka Kishimoto, Sumiko Kanahori, Hiroshi Hara
    • Organizer
      10th Vahouny Dietary Fiber Symposium, The American Society for Nutrition and NutraSource, Inc
    • Place of Presentation
      Marriott Bethesda North, Bethesda, USA
    • Year and Date
      2014-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 難消化性糖質摂取ラットにおける食品ペプチド経口投与に対するGLP-1分泌2013

    • Author(s)
      池江明日香、比良徹、岸本由香、原博
    • Organizer
      日本食物繊維学会18回学術集会
    • Place of Presentation
      仁愛大学(福井県越前市)
    • Year and Date
      2013-11-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 食品ペプチドの消化管での作用を介した血糖制御に関する研究2013

    • Author(s)
      比良徹
    • Organizer
      2013年度日本アミノ酸学会科学・技術賞受賞講演、日本アミノ酸学会
    • Place of Presentation
      熊本市医師会館(熊本市)
    • Year and Date
      2013-11-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 難消化性デキストリン摂取は、ラット血中GLP-1濃度を高め、耐糖能を改善する2013

    • Author(s)
      池江明日香、比良徹、岸本由香、原博
    • Organizer
      第34回日本肥満学会
    • Place of Presentation
      東京フォーラム(東京)
    • Year and Date
      2013-10-11
    • Related Report
      2013 Final Research Report
  • [Presentation] PHYSIOLOGICAL FUNCTIONS OF SOYBEAN PEPTIDES, Diversity of physionlogical functions of amino acids and peptide2013

    • Author(s)
      Hara H; Nakajima S; Hira T
    • Organizer
      20th International Congress of Nutrition (ICN2013)
    • Place of Presentation
      Granada Congress, Granada, Spain
    • Year and Date
      2013-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットにおいて難消化性デキストリンの長期摂取は血中GLP-1濃度を高める2013

    • Author(s)
      池江明日香、比良徹、岸本由香、原博
    • Organizer
      第67回日本栄養・食糧学会大会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2013-05-25
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットにおいてトウモロコシペプチドの経口投与が、インクレチン分泌促進を介して血糖上昇を抑制する2013

    • Author(s)
      比良徹、樋口謹行、原博
    • Organizer
      第56回日本糖尿病学会年次学術集会
    • Place of Presentation
      テトリア熊本ビル(熊本市)
    • Year and Date
      2013-05-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Fructooligosaccharides augments the effect of a flavonoid, a-glucosyl-isoquercitrin, on glucagon-like peptide 1 secretion2013

    • Author(s)
      P. Phuwamongkolwiwat, T. Hira and H. Hara
    • Organizer
      Experimental Biology 2013
    • Place of Presentation
      Boston Convention & Exibition Center, Boston, USA
    • Year and Date
      2013-04-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Fructooligosaccharides augments the effect of a flavonoid, a-glucosyl-isoquercitrin, on glucagon-like peptide 1 secretion.2013

    • Author(s)
      P. Phuwamongkolwiwat, T. Hira and H. Hara
    • Organizer
      Experimental Biology 2013
    • Place of Presentation
      Boston Convention & Exibition Center, Boston, MA, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] ラットにおいてトウモロコシペプチドの経口投与が、インクレチン分泌促進を介して血糖上昇を抑制する2013

    • Author(s)
      比良 徹、樋口 謹行、原 博
    • Organizer
      第56回日本糖尿病学会年次学術集会
    • Place of Presentation
      テトリア熊本ビル、熊本市
    • Related Report
      2012 Annual Research Report
  • [Presentation] ラットにおいて難消化性デキストリンの長期摂取は血中GLP-1濃度 を高める"2013

    • Author(s)
      池江 明日香、比良 徹、岸本 由香、原 博
    • Organizer
      第67回日本栄養・食糧学会大会
    • Place of Presentation
      名古屋大学、名古屋市
    • Related Report
      2012 Annual Research Report
  • [Presentation] "難消化性デキストリン摂取は、 ラット血中GLP-1濃度を高め、耐糖能を改善する "2013

    • Author(s)
      池江 明日香、比良 徹、岸本 由香、原 博
    • Organizer
      第34回日本肥満学会
    • Place of Presentation
      東京フォーラム、東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 難消化性糖質摂取ラットにおける食品ペプチド経口投与に対する GLP-1 分泌2013

    • Author(s)
      池江 明日香、比良 徹、岸本 由香、原 博
    • Organizer
      日本食物繊維学会 18回学術集会
    • Place of Presentation
      仁愛大学、福井県越前市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 難消化性デキストリンの単回及び長期摂取はラットのGLP-1分泌を促進する2012

    • Author(s)
      池江明日香、比良徹、岸本由香、原博
    • Organizer
      日本食物繊維学会17回学術集会
    • Place of Presentation
      中村学園大学(福岡市)
    • Year and Date
      2012-11-23
    • Related Report
      2013 Final Research Report
  • [Presentation] 難消化性デキストリンのラット腸管におけるGLP-1分泌作用の検討2012

    • Author(s)
      池江明日香、比良徹、岸本由香、原博
    • Organizer
      第66回日本栄養・食糧学会大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2012-05-29
    • Related Report
      2013 Final Research Report
  • [Presentation] トウモロコシZein加水分解物の経口投与はインクレチン分泌を介して血糖上昇を抑制する2012

    • Author(s)
      樋口謹行、比良徹、原博
    • Organizer
      第66回日本栄養・食糧学会大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2012-05-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Fructooligosaccharides enhance bene?cial effects of a?avonoid, quercetin-3-glucoside, on insulin sensitivity in rats fed high sucrose diet2012

    • Author(s)
      P Phuwamongkolwiwat, T Suzuki, T Hira and H Hara
    • Organizer
      Experimental Biology 2012
    • Place of Presentation
      San Diego Convention Center, San Diego, USA
    • Year and Date
      2012-04-23
    • Related Report
      2013 Final Research Report
  • [Presentation] トウモロコシたんぱく質Zeinの加水分解物による血糖上昇抑制へのGLP-1の関与2011

    • Author(s)
      樋口謹行,比良徹,原博
    • Organizer
      日本食物繊維学会16回学術集会
    • Place of Presentation
      独立行政法人国立健康・栄養研究所(東京都)
    • Year and Date
      2011-11-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 難消化性デキストリンは消化管内分泌細胞に直接作用してGLP-1分泌を促進する2011

    • Author(s)
      池江明日香、比良徹、岸本由香、原博
    • Organizer
      日本食物繊維学会16回学術集会
    • Place of Presentation
      独立行政法人国立健康・栄養研究所(東京都)
    • Year and Date
      2011-11-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 難消化性デキストリンのGLP-1分泌作用の検討2011

    • Author(s)
      池江明日香、比良徹、岸本由香、原博
    • Organizer
      第41回日本栄養・食糧学会北海道支部会
    • Place of Presentation
      天使大学(札幌市)
    • Year and Date
      2011-10-23
    • Related Report
      2013 Final Research Report
  • [Presentation] トウモロコシZein加水分解物はラットへの経口投与によりインクレチン分泌を介して血糖上昇を抑制する2011

    • Author(s)
      樋口謹行、比良徹、原博
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      お茶の水女子大学(東京都)
    • Year and Date
      2011-05-15
    • Related Report
      2013 Final Research Report
  • [Presentation] トウモロコシ Zein 加水分解物はラットへの経口投与によりインクレチン分泌を介して血糖上昇を抑制する2011

    • Author(s)
      樋口 謹行 、比良 徹、原 博
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      お茶の水女子大学(東京都)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 難消化性デキストリンのGLP-1分泌作用の検討2011

    • Author(s)
      池江 明日香、比良 徹、岸本 由香、原 博
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      お茶の水女子大学(東京都)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] トウモロコシたんぱく質 Zein の加水分解物による血糖上昇抑制への GLP-1 の関与2011

    • Author(s)
      樋口謹行,比良 徹,原 博
    • Organizer
      日本食物繊維学会 16 回学術集会
    • Place of Presentation
      独立行政法人国立健康・栄養研究所(東京都)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 難消化性デキストリンは消化管内分泌細胞に直接作用して GLP-1 分泌を促進する2011

    • Author(s)
      池江明日香、比良 徹、岸本由香、原 博
    • Organizer
      日本食物繊維学会 16 回学術集会
    • Place of Presentation
      独立行政法人国立健康・栄養研究所(東京都)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] ラットにおけるトウモロコシたんぱく加水分解物の経口投与による血糖上昇抑制2010

    • Author(s)
      樋口謹行、比良徹、原博
    • Organizer
      第8回日本栄養改善学会北海道支部学術総会
    • Place of Presentation
      天使大学(札幌)
    • Year and Date
      2010-12-04
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] トウモロコシZein加水分解物はラットへの経口投与により血糖上昇を抑制する2010

    • Author(s)
      樋口謹行、比良徹、原博
    • Organizer
      第40回日本栄養・食糧学会北海道支部会
    • Place of Presentation
      天使大学(札幌)
    • Year and Date
      2010-10-24
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] パラチノースによるGLP-1分泌促進機構の解析2010

    • Author(s)
      比良徹、村松茉耶、奥野雅浩、原博
    • Organizer
      第31回日本肥満学会
    • Place of Presentation
      前橋テルサ(群馬県)
    • Year and Date
      2010-10-02
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] ラットにおけるトウモロコシZein加水分解物によるGLP-1分泌を介した血糖上昇抑制2010

    • Author(s)
      比良徹、持田泰輔、原博
    • Organizer
      日本アミノ酸学会第4回学術大会
    • Place of Presentation
      ホテルサンシャイン鬼怒川(栃木県)
    • Year and Date
      2010-09-17
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] 食品ペプチドによる消化管ホルモン分泌について?消化管の栄養素受容機構2010

    • Author(s)
      比良徹
    • Organizer
      Hindgut Clubサテライトミーティング(第64回日本栄養・食糧学会関連学術集会)
    • Place of Presentation
      アスティ徳島(徳島県)
    • Year and Date
      2010-05-22
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 食品ペプチドによる消化管ホルモン分泌について~消化管の栄養素受容機構2010

    • Author(s)
      比良徹
    • Organizer
      Hindgut Clubサテライトミーティング(第64回日本栄養・食糧学会関連学術集会)
    • Place of Presentation
      アスティ徳島(徳島県) 招待講演
    • Year and Date
      2010-05-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Corn protein hydrolysate stimulates GLP-1 secretion and attenuates hyperglycaemia in rats2010

    • Author(s)
      T. Hira, T. Mochida, H. Hara
    • Organizer
      The 18th International Symposium on Regulatory Peptides
    • Place of Presentation
      Europa Hotel Belfast (Belfast, UK)
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] Effects of resistant maltodextrin and fructooligosaccharides on fasting GLP-1 levels and glucose tolerance in rats

    • Author(s)
      Tohru Hira, Asuka Ikee, Yuka Kishimoto, Sumiko Kanahori, Hiroshi Hara
    • Organizer
      10th Vahouny Dietary Fiber Symposium
    • Place of Presentation
      Marriott Bethesda North, Bethesda, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] PHYSIOLOGICAL FUNCTIONS OF SOYBEAN PEPTIDE

    • Author(s)
      Hara H; Nakajima S; Hira T
    • Organizer
      20th International Congress of Nutrition (ICN2013)
    • Place of Presentation
      Granada Congress Center, Granada, Spain
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://www.agr.hokudai.ac.jp/shokuei/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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