Analysis of impairment and recovery mechanism of intestinal barrier and its application for design of foods with barrier-protectingactivity

• Project/Area Number: 22380076
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Hiroshima University
• Principal Investigator: TANABE Soichi 広島大学, 大学院・生物圏科学研究科, 教授 (90272624)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Takuya 広島大学, 大学院・生物圏科学研究科, 講師 (30526695)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: 腸管バリア / タイトジャンクション / Th17 / 乳酸菌 / ビフィズス菌 / D-アラニン / 乳酸菌・ビフィズス菌 / フラボノイド

Research Abstract

Caco-2 cells were used as a human intestinal model. Tight-junction (TJ) of the cells was impaired by the addition of cytokine (TNF-alpha). A milk casein-derived peptide, NPWDQ, recovered the impairment by, at least in part, increasing the expression of occludin, one of TJ proteins. Some lactic acid bacteria and bifidobacteria also exerted the barrier-recovering activity. Itwas found that D-alanine in bacterial cell wall played an important role in the barrier-protecting effects of Streptococcus thermophilusin Caco-2 cells. This finding would be applicable for design of foods with barrier-protecting activity. In addition, Bifidobacterium longumameliorated colitis in mice.

Research Products

• [Journal Article] Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells (2012)
  • Author(s): Miyauchi E, Morita M, Rossi M, Morita H, Suzuki T, Tanabe S
  • Journal Title: Biosci Biotechnol Biochem Volume: 76 Pages: 283-288
  • NAID: 10030399670
  • Peer Reviewed
• [Journal Article] Short peptide modules for enforcing intestinal barrier (2012)
  • Author(s): Tanabe S
  • Journal Title: Current Pharmaceutical Design Volume: 18 Pages: 776-781
  • Peer Reviewed
• [Journal Article] Effect of D-Alanine in Teichoic Acid from the Streptococcus thermophilus Cell Wall on the Barrier-Protection of Intestinal Epithelial Cells (2012)
  • Author(s): Miyauchi E, Morita M, Rossi M, Morita H, Suzuki T, Tanabe S
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 2 Pages: 283-288
  • DOI: 10.1271/bbb.110646
  • NAID: 10030399670
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Mechanism of protection of transepithelial barrier function by Lactobacillus salivarius: strain dependence and attenuation by bacteriocin production (2012)
  • Author(s): Eiji MIYAUCHI et al.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 303 Issue: 9 Pages: G1029-G1041
  • DOI: 10.1152/ajpgi.00003.2012
  • Peer Reviewed
• [Journal Article] Short peptide modules for enhancing intestinal barrier function (2012)
  • Author(s): Soichi TANABE
  • Journal Title: Curr Pharm Des Volume: 18 Pages: 776-781
  • Peer Reviewed
• [Journal Article] Suppression of Th17 response by Streptococcus thermophilus ST28 through induction of IFN-γ (2011)
  • Author(s): Ogita T, Tanii Y, Morita H, Suzuki T, Tanabe S
  • Journal Title: Int J Mol Med Volume: 28 Pages: 817-822
  • Peer Reviewed
• [Journal Article] Streptococcus thermophilusST28 ameliorates colitis in mice partially by suppression of inflammatory Th17 cells (2011)
  • Author(s): Ogita T, Nakashima M, Morita H, Saito Y, Suzuki T, Tanabe S
  • Journal Title: J Biomed Biotechnol
  • Peer Reviewed
• [Journal Article] Interleukin-6 (IL-6) regulates claudin-2 expression and tight junction permeability in intestinal epithelium (2011)
  • Author(s): Suzuki T, Yoshinaga N, Tanabe S
  • Journal Title: J Biol Chem Volume: 286 Pages: 1263-1271
  • Peer Reviewed
• [Journal Article] Suppression of Th17 response by Streptococcus thermopbilus ST28 through induction of IFN-γ (2011)
  • Author(s): Tasuku Ogita, Yusuke Tanii, Hidetoshi Morita, Takuya Suzuki, Soichi Tanabe
  • Journal Title: Int J Mol Med Volume: 28 Pages: 817-822
  • DOI: 10.3892/ijmm.2011.755
  • Peer Reviewed
• [Journal Article] Streptococcus thermophilus ST28 ameliorates colitis in mice partially by suppression of inflammatory Th17 cells (2011)
  • Author(s): Tasuku Ogita, Megumi Nakashima, Hidetoshi Morita, Yasuo Saito, Takuya Suzuki, Soichi Tanabe
  • Journal Title: J Biomed Biotechnol Volume: 2011 Issue: 1 Pages: 378417-378417
  • DOI: 10.1155/2011/378417
  • Peer Reviewed
• [Journal Article] Kaempferol enhances intestinal barrier function through the cytoskeletal association and expression of tight junction proteins in Caco-2 cells (2011)
  • Author(s): Takuya SUZUKI, Soichi TANABE, Hiroshi HARA
  • Journal Title: Journal of Nutrition Volume: 141 Pages: 87-94
  • Peer Reviewed
• [Journal Article] The casein peptide NPWDQ enforces the intestinal tight junction partly by increasing occludin expression in Caco-2 cells (2010)
  • Author(s): Yasumatsu H, Tanabe S
  • Journal Title: Br J Nutr Volume: 104 Pages: 951-956
  • Peer Reviewed
• [Journal Article] Oral administration of a galactooligosaccharide preparation inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice (2010)
  • Author(s): Soichi TANABE, Satomi HOCHI
  • Journal Title: International Journal of Molecular Medicine Volume: 25 Pages: 331-336
  • Peer Reviewed
• [Journal Article] The casein peptide NPWDQ enforces the intestinal tight junction partly by increasing occludin expression in Caco-2 cells (2010)
  • Author(s): Hatsumi YASUMATSU, Soichi TANABE
  • Journal Title: British Journal of Nutrition Volume: 104 Pages: 951-956
  • Peer Reviewed
• [Presentation] Streptococcus thermophilus ST28 ameliorates colitis in mice by suppression of inflammatory Th17 cells (2012)
  • Author(s): Tanabe S
  • Organizer: 4th International Symposium of Lactic Acid Bacteria(招待講演)
  • Place of Presentation: プサン(韓国)
  • Year and Date: 2012-12-02
• [Presentation] 粘膜バリア保護素材の開発的研究 (2012)
  • Author(s): 田辺 創一
  • Organizer: 日本食品免疫学会第5回シンポジウム(招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2012-06-07
• [Presentation] ナリンゲニンによる大腸バリア機能保護作用と炎症抑制作用は、他のポリフェノール(ケルセチン、クルクミン、ヘスペレチン)よりも強い (2012)
  • Author(s): 重白みづき、野田咲乃、田辺創一、鈴木卓弥
  • Organizer: 日本農芸化学会2012年大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-24
• [Presentation] 生もと乳酸菌による皮膚炎症抑制効果 (2012)
  • Author(s): 永汐祐紀、竹之下柳一朗、渡辺敏郎、石知史、高橋俊成、近藤紗代、鈴木卓弥、田辺創一
  • Organizer: 日本農芸化学会2012年大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] 生もと乳酸菌を用いた米ヌカ発酵液のヒアルロン酸産生促進効果 (2012)
  • Author(s): 近藤紗代、増田康之、高橋俊成、渡辺敏郎、石知史、田辺創一、溝口晴彦
  • Organizer: 日本農芸化学会2012年大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] ピーナッツおよびソバアレルゲンのIgE結合交叉性エピトープの解析 (2012)
  • Author(s): 勝山真多、岡田晋治、小林彰子、田辺創一
  • Organizer: 日本農芸化学会2012年大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] 腸管バリア調節機能を有するミルクペプチド (2011)
  • Author(s): 田辺 創一
  • Organizer: 第32回機能性食品用ペプチド研究会(招待講演)
  • Place of Presentation: 大阪
  • Year and Date: 2011-12-19
• [Presentation] 腸管バリア調節機能を有するミルクペプチド (2011)
  • Author(s): 田辺創一
  • Organizer: 第32回機能性食品用ペプチド研究会
  • Place of Presentation: 大阪(招待講演)
  • Year and Date: 2011-12-19
• [Presentation] Streptococcus thermophilus ST28 ameliorates colitis in mice by suppression of inflammatory Th17 cells (2011)
  • Author(s): Soichi Tanabe
  • Organizer: 4th International Symposium of Lactic Acid Bacteria
  • Place of Presentation: プサン(韓国)(招待講演)
  • Year and Date: 2011-12-02
• [Presentation] 乳酸菌のリポテイコ酸による腸管バリア保護効果 (2011)
  • Author(s): 宮本潤基, 森田真帆, 宮内栄治, 鈴木卓弥, 田辺創一
  • Organizer: 日本畜産学会 第113回大会
  • Place of Presentation: 厚木市
  • Year and Date: 2011-03-29
• [Presentation] Streptococcus thermophilus ST28によるDSS誘導腸炎マウスの腸炎抑制効果 (2011)
  • Author(s): 荻田佑, 谷井勇介, 中島恵, 森田英利, 鈴木卓弥, 田辺創一
  • Organizer: 日本畜産学会 第113回大会
  • Place of Presentation: 厚木市
  • Year and Date: 2011-03-29
• [Presentation] Bifidobacterium longum BB536含有ヨーグルト摂取によるEnterotoxigenic Bacteroides fragilis (ETBF)保菌者への作用 (2011)
  • Author(s): 小田巻俊孝, 菅原宏祐, 米澤寿美子, 清水(肖)金忠, 八重島智子, 岩附慧二, 田辺創一, 富永友也, 富樫秀生, 辨野義己
  • Organizer: 日本農芸化学会 2011年度大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-03-27
• [Presentation] ビフィズス菌によるヒト末梢血単核球における炎症性インターロイキン(IL)-17産生抑制 (2011)
  • Author(s): 荻田佑, 石川裕也, 清水金忠, 岩淵紀介, 森田英利, 鈴木卓弥, 田辺創一
  • Organizer: 日本農芸化学会 2011年度大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-03-26
• [Presentation] 生もと乳酸菌による皮膚細胞における保護効果 (2011)
  • Author(s): 荻田佑, 永汐祐紀, 宮本潤基, 渡辺敏郎, 高橋俊成, 吉田和利, 鈴木卓弥, 田辺創一
  • Organizer: 日本農芸化学会 2011年度大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-03-26
• [Presentation] フラバノンの消化管細胞および人工脂質膜における吸収機作の解析 (2011)
  • Author(s): 小林彰子, 永井俊匡, 小西豊, 田辺創一, 森本かおり, 荻原琢男
  • Organizer: 日本農芸化学会 2011年度大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-03-26
• [Presentation] A citrus flavonoid hesperidin inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice via modulating Treg/Th17 balance (2010)
  • Author(s): Nagashio Y, Suzuki T, Tanabe S
  • Organizer: International Conference on Regulatory T Cells and Th17 Cells and Clinical Application in Human Diseases
  • Place of Presentation: 上海(中国)
  • Year and Date: 2010-07-17
• [Presentation] A citrus flavonoid hesperidin inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice via modulating Treg/Th17 balance (2010)
  • Author(s): Yuuki NAGASHIO, Takuya SUZUKI, Soichi TANABE
  • Organizer: International Conference on Regulatory T Cells and Th17 Cells and Clinical Application in Human Diseases
  • Place of Presentation: 中国・上海
  • Year and Date: 2010-07-17
• [Presentation] Lipoteichoic acid of lactic acid bacteria ameliorates intestinal barrier impairment and inflammation (2010)
  • Author(s): Tanabe S
  • Organizer: Annual World Congress of Immunodiseases and Therapy(招待講演)
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-05-15
• [Presentation] Lipoteichoic acid of lactic acid bacteria ameliorates intestinal barrier impairment and inflammation (2010)
  • Author(s): Soichi TANABE
  • Organizer: Annual World Congress of Immunodiseases and Therapy
  • Place of Presentation: 中国・北京(招待講演)
  • Year and Date: 2010-05-15
• [Presentation] 粘膜バリア保護素材の開発的研究
  • Author(s): 田辺 創一
  • Organizer: 日本食品免疫学会シンポジウム
  • Place of Presentation: 東京
  • Invited
• [Presentation] 高度不飽和脂肪酸の乳酸菌代謝産物はCaco-2細胞における腸管上皮バリアの損傷を回復する
  • Author(s): 宮本 潤基
  • Organizer: 日本農芸化学会全国大会
  • Place of Presentation: 仙台
• [Presentation] 乳酸菌AYA株に含まれるIgA産生促進物質
  • Author(s): 吉田 光
  • Organizer: 日本畜産学会全国大会
  • Place of Presentation: 広島
• [Presentation] 乳酸菌のＭ２マクロファージ分化促進作用
  • Author(s): 竹之下 柳一朗
  • Organizer: 日本畜産学会全国大会
  • Place of Presentation: 広島
• [Book] Genomics and Molecular Aspects (2010)
  • Author(s): Miyauchi E, Xiao J, Tanabe S
  • Publisher: Caister Academic Press, Bifidobacteria
• [Book] ルミナコイド研究のフロンティア (2010)
  • Author(s): 田辺 創一
  • Publisher: 建帛社
• [Book] Bifidobacteria : Genomics and Molecular Aspects (2010)
  • Author(s): Eiji MIYAUCHI, Jin-zhong XIAO, Soichi TANABE
  • Total Pages: 259
  • Publisher: Caister Academic Press
• [Book] ルミナコイド研究のフロンティア (2010)
  • Author(s): 田辺創一
  • Total Pages: 268
  • Publisher: 建帛社