Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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Research Abstract |
Caco-2 cells were used as a human intestinal model. Tight-junction (TJ) of the cells was impaired by the addition of cytokine (TNF-alpha). A milk casein-derived peptide, NPWDQ, recovered the impairment by, at least in part, increasing the expression of occludin, one of TJ proteins. Some lactic acid bacteria and bifidobacteria also exerted the barrier-recovering activity. Itwas found that D-alanine in bacterial cell wall played an important role in the barrier-protecting effects of Streptococcus thermophilusin Caco-2 cells. This finding would be applicable for design of foods with barrier-protecting activity. In addition, Bifidobacterium longumameliorated colitis in mice.
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