Analysis of impairment and recovery mechanism of intestinal barrier and its application for design of foods with barrier-protectingactivity
Project/Area Number |
22380076
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Food science
|
Research Institution | Hiroshima University |
Principal Investigator |
TANABE Soichi 広島大学, 大学院・生物圏科学研究科, 教授 (90272624)
|
Co-Investigator(Kenkyū-buntansha) |
SUZUKI Takuya 広島大学, 大学院・生物圏科学研究科, 講師 (30526695)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
|
Keywords | 腸管バリア / タイトジャンクション / Th17 / 乳酸菌 / ビフィズス菌 / D-アラニン / 乳酸菌・ビフィズス菌 / フラボノイド |
Research Abstract |
Caco-2 cells were used as a human intestinal model. Tight-junction (TJ) of the cells was impaired by the addition of cytokine (TNF-alpha). A milk casein-derived peptide, NPWDQ, recovered the impairment by, at least in part, increasing the expression of occludin, one of TJ proteins. Some lactic acid bacteria and bifidobacteria also exerted the barrier-recovering activity. Itwas found that D-alanine in bacterial cell wall played an important role in the barrier-protecting effects of Streptococcus thermophilusin Caco-2 cells. This finding would be applicable for design of foods with barrier-protecting activity. In addition, Bifidobacterium longumameliorated colitis in mice.
|
Report
(4 results)
Research Products
(41 results)