| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Research Abstract |
To know the dispersal and reproductive mechanisms of tree pathogenic fungi, we developed polymorphic microsatellite markers of Raffaelea quercivora, Taphrina wiesneri, and Perenniporia fraxinea. Using these markers, we investigated the genetic structure of these fungi in seriously damaged sites. In addition, we investigated distribution of Armillaria tabescens, Ganoderma applanatum, and P. fraxinea within the trunk of infested trees using T-RFLP analysis. We also examined finer scale hypha distribution in the xylem of infested trees using hyphal staining with F-WGA.
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