Molecular and physiological studies on some ectomycorrhizal symbiotic fungi

• Project/Area Number: 22380084
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Forest science
• Research Institution: Kyoto University
• Principal Investigator: TANAKA Chihiro 京都大学, 大学院・農学研究科, 准教授 (60263133)
• Co-Investigator(Kenkyū-buntansha): HIRAI Nobuhiro 京都大学, 農学研究科, 教授 (00165151)
• Research Collaborator: 太田 明 滋賀県森林センター, 専門員 ; 河合 昌孝 奈良県森林技術センター, 総括研究員
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 外生菌根菌 / ホンシメジ / バカマツタケ / マツタケ / ハタケシメジ / Agrobacterium / 形質転換 / ゲノム / きのこ / 菌根 / 半数体 / 胞子発芽 / 酪酸 / ショウロ / 相同組換え

Research Abstract

In this study, Japanese ectomycorrhizal fungi, e.g. Tricholoma matsutake， T.bakamatsutake(an allies of T. matsutakewith different host spectrum)，Lyophylum shimejiand L. decastes(saprophytic and phylogenetically closely related species of L. shimeji) were featured. We isolated haploid strains of these fungal species bysingle basidiospore isolations, and compared their phenotypes on colonial growth and ectomycorrhizal formations etc. We also tested competency of these fungal species for Agro-infections. Finally, we chose a representative haploid strain of L. shimejiand L. decastes, respectively. The genomes were sequenced using HiSeq2000 system.

Research Products

• [Journal Article] Transformation of the mushroom species Hypsizigus marmoreus, Flammulina velutipes, and Grifola frondosa by an Agrobacterium-mediated method using a universal transformation plasmid (2013)
  • Author(s): K. Hatoh, K. Izumitsu, A. Morita, K. Shimizu, A. Ohta, M. Kawai, T. Yamanaka, H. Neda, Y. Ota, C. Tanaka
  • Journal Title: Mycoscience Volume: 54 Issue: 1 Pages: 8-12
  • DOI: 10.1016/j.myc.2012.08.002
  • Peer Reviewed
• [Journal Article] Phylogenetic relationship and species delimitation of matsutakeandallied species based on multilocus phylogeny and haplotype analyses (2012)
  • Author(s): Ota Y, Yamanaka T, Murata H, Neda H, Ohta A, Kawai M, Yamada A, Konno M, Tanaka C
  • Journal Title: Mycologia Volume: 104 Issue: 6 Pages: 1368-1380
  • DOI: 10.3852/12-068
  • Peer Reviewed
• [Journal Article] Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR (2012)
  • Author(s): Izumitsu K, Hatoh K, Sumita T, Kitade Y, Morita A, Gafur A, Ohta A, Kawai M, Yamanaka T, Neda H, Ota Y, Tanaka C
  • Journal Title: Mycoscience Volume: (未定)(確定) Issue: 5 Pages: 396-401
  • DOI: 10.1007/s10267-012-0182-3
  • Peer Reviewed
• [Journal Article] Genetic diversity of Tricholoma matsutakeand close allies associated with broad-leaved trees in Asia (2011)
  • Author(s): Wan J, Koike A, Yamanaka K, Sotome K, Morinaga T, Tanaka C, Terashima Y, Aimi T
  • Journal Title: Mushroom Science and Biotechnology Volume: 19 Pages: 167-174
  • Peer Reviewed
• [Journal Article] Coprinopsis neophlyctidosporasp. nov., a new ammonia fungus from boreal forests in Canada (2011)
  • Author(s): Raut JK, Suzuki A, Fukiharu T, Shimizu K, Kawamoto S, Tanaka C
  • Journal Title: Mycotaxon Volume: 115 Issue: 1 Pages: 227-238
  • DOI: 10.5248/115.227
  • Peer Reviewed
• [Journal Article] Coprinopsis austrophlyctidosporasp. nov., an agaric ammonia fungus from southern hemisphere plantations and natural forests (2011)
  • Author(s): Fukiharu T, Bougher NL, Buchanan PK, Suzuki A, Tanaka C, Sagara N
  • Journal Title: Mycoscience Volume: 52 Issue: 2 Pages: 137-142
  • DOI: 10.1007/s10267-010-0077-0
  • NAID: 10028174711
  • Peer Reviewed
• [Presentation] 汎用的な遺伝子組換えプラスミドを用いたアグロバクテリウム法によるブナシメジ,エノキタケおよびマイタケの遺伝子組換え (2012)
  • Author(s): 羽當加奈子,泉津弘佑,森田篤,清水公徳,太田明,河合昌孝 ,山 中 高 史 ,根田仁,太田祐子,田中千尋
  • Organizer: 日本菌学会2012年度大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2012-05-27
• [Presentation] 植物病原糸状菌の遺伝生理生態学的研究 (2012)
  • Author(s): 田中千尋
  • Organizer: 日本菌学会2012年度大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2012-05-26
• [Presentation] 汎用的な遺伝子組換えプラスミドを用いたアグロバクテリウム法によるブナシメジ,エノキタケおよびマイタケの遺伝子組換え (2012)
  • Author(s): 羽當 加奈子
  • Organizer: 日本菌学会
  • Place of Presentation: 岐阜大学 農学部
• [Presentation] 植物病原糸状菌の遺伝生理生態学的研究 (2012)
  • Author(s): 田中 千尋
  • Organizer: 日本菌学会
  • Place of Presentation: 岐阜大学 農学部
  • Invited
• [Presentation] Cytological study in Tricholoma bakamatsutake (2010)
  • Author(s): Tanaka C, KawaiM, OhtaA, YamazakiM, OhtaY, YamanakaT, Neda H
  • Organizer: International Mycological Congress 9
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2010-08-04
• [Remarks]
  • URL: http://remach.kais.kyoto-u.ac.jp/~ems