| Project/Area Number |
22380084
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Kyoto University |
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Principal Investigator |
TANAKA Chihiro 京都大学, 大学院・農学研究科, 准教授 (60263133)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Nobuhiro 京都大学, 農学研究科, 教授 (00165151)
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| Research Collaborator |
太田 明 滋賀県森林センター, 専門員
河合 昌孝 奈良県森林技術センター, 総括研究員
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | 外生菌根菌 / ホンシメジ / バカマツタケ / マツタケ / ハタケシメジ / Agrobacterium / 形質転換 / ゲノム / きのこ / 菌根 / 半数体 / 胞子発芽 / 酪酸 / ショウロ / 相同組換え |
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| Research Abstract |
In this study, Japanese ectomycorrhizal fungi, e.g. Tricholoma matsutake， T.bakamatsutake(an allies of T. matsutakewith different host spectrum)，Lyophylum shimejiand L. decastes(saprophytic and phylogenetically closely related species of L. shimeji) were featured. We isolated haploid strains of these fungal species bysingle basidiospore isolations, and compared their phenotypes on colonial growth and ectomycorrhizal formations etc. We also tested competency of these fungal species for Agro-infections. Finally, we chose a representative haploid strain of L. shimejiand L. decastes, respectively. The genomes were sequenced using HiSeq2000 system.
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