| Project/Area Number |
22380105
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | 財団法人目黒寄生虫館 (2012)
Meguro Parasitological Museum (2011)
The University of Tokyo (2010) |
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Principal Investigator |
OGAWA Kazuo 財団法人目黒寄生虫館, 館長 (20092174)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHINAGA Tomoyoshi 東京大学, 農学生命科学研究科, 教授 (20345185)
SAWADA Yoshifumi 近畿大学, 水産研究所, 教授 (80319764)
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| Co-Investigator(Renkei-kenkyūsha) |
ISIMARU Katsuya 近畿大学, 水産研究所, 助教 (00330241)
SHIRAKASHI Sho 近畿大学, 水産研究所, 講師 (70565936)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Keywords | 住血吸虫 / クロマグロ / Cardicola / プラジクアンテル / 対策 / 害作用 / Psettarium / in vitro飼育 |
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| Research Abstract |
Firstly, a second blood fluke species from Pacific bluefin tuna was found from the heart ventricle and described as Cardicola opisthorchis n. sp. Secondly, new methods were developed to distinguish eggs of C. orientalis from those of C. opisthorchis morphologically, and to count the number of eggs in the gills. Thirdly, infection cycle was partly elucidated. Namely, both species of blood flukes were found in juvenile tuna 45 days after transfer to outside net cages; one week later, flukes were matured and laid eggs after one more week. Fourthly, C. opisthorchis was eradicated from tuna with oral administration of praziquantel (PZQ) at 7.5 mg/kg fish body weight or more for 3 consecutive days. Reinfection was confirmed 1 month after medication. PZQ concentration in tuna decreased to the undetectable level（0.02 μg/L）24 h after administration. Lastly, SEM observation of the two species of blood flukes shows swelling and contraction of the tegument and formation of many minute furrowed ridges in C. opisthorchis, while in C. orientalis, many minute holes were characteristically formed on the tegument.
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