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Study on impact and pathobiology of Perkinsus infection in wild Manila clam for recovery of Manila clam resources

Research Project

Project/Area Number 22380106
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionThe University of Tokyo

Principal Investigator

YOSHINAGA Tomoyoshi  東京大学, 大学院・農学生命科学研究科, 教授 (20345185)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywordsアサリ / Perkinsus / 病害 / 資源 / 原虫 / 野生集団 / 減耗要因 / パーキンサス
Research Abstract

Experimental challenge of Manila clam with the protozoan Perkinsus olseni revealed that this protozoan was lethal to the host and that the lethal infection intensity to Manila clam was around 106 cells/g soft tissue weight. Three-year monitoring of the infection level of the protozoan and the dynamics of a host population was carried out at one location in Ariake Bay where Manila clam population has dramatically decreased since late 1970s. This monitoring demonstrated that the infection prevalence reached 100 % and the population sizes of Manila clams decreased when the highest infection intensities reached the lethal infection intensity experimentally obtained. The results strongly suggested that infection with the protozoa was the major factor for the depletion of Manila clam resources.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (20 results)

All 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (15 results)

  • [Journal Article] Effects of temperature and salinity on the in vitro proliferation of trophozoites and the development of Zoosporangia in Perkinsus olseni and P. honshuensis, both Infecting Manila clam2013

    • Author(s)
      Umeda, K., Shimokawa, J., Yoshinaga, T
    • Journal Title

      Fish Pathology

      Volume: 48 Pages: 13-16

    • NAID

      10031162769

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Temperature and Salinity on the in vitro Proliferation of Trophozoites and the Development of Zoosporangia in Perkinsus olseni and P. honshuensis, Both Infecting Manila clam.2013

    • Author(s)
      Umeda, K., Shimokawa, J., Yoshinaga,
    • Journal Title

      Fish Pathology

      Volume: 48(1) Pages: 13-16

    • NAID

      10031162769

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of real-time PCR assays for two Perkinsus spp. in the Manila clam Ruditapes philippinarum2012

    • Author(s)
      Umeda, K., Yoshinaga, T
    • Journal Title

      Diseases of Aquatic Organisms

      Volume: 99 Pages: 215-225

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Experimental challenges of wild Manila clams with Perkinsus species isolated from naturally infected wild Manila clams2012

    • Author(s)
      Waki, T., Shimokawa, J., Watanabe, S., Yoshinaga, T., Ogawa, K
    • Journal Title

      Journal of Invert.Pathology

      Volume: 111 Pages: 50-55

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of real-time PCR assays for two Perkinsus spp. in the Manila clam Ruditapes philippinarum2012

    • Author(s)
      Umeda, K., Yoshinaga, T.
    • Journal Title

      Diseases of Aquatic Organisms

      Volume: 99(3) Issue: 3 Pages: 215-225

    • DOI

      10.3354/dao02476

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 有明海定点におけるPerkinsus属原虫のアサリの減耗への関与2013

    • Author(s)
      脇司・良永知義
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] アサリのPerkinsus属原虫の分布に影響する環境要因について2013

    • Author(s)
      梅田剛佑・高橋美希・脇司・悦喜達也・良永知義
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] アサリに寄生するPerkinsus属原虫2種の国内分布2013

    • Author(s)
      梅田剛佑・良永知義
    • Organizer
      平成25年度日本魚病学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-09
    • Related Report
      2012 Final Research Report
  • [Presentation] アサリに寄生するPerkinsus属原虫の病害性の実験的な評価2013

    • Author(s)
      脇司・良永知義
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2013-02-06
    • Related Report
      2012 Final Research Report
  • [Presentation] アサリに寄生するPerkinsus属原虫2種の国内分布2013

    • Author(s)
      梅田剛佑・良永知義
    • Organizer
      平成25年度日本魚病学会春季大会
    • Place of Presentation
      日本大学生物資源科学部湘南キャンパス(神奈川県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] アサリに寄生する Perkinsus 属原虫の病害性の実験的な評価2013

    • Author(s)
      脇 司・良永知義
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(品川キャンパス)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 有明海定点における Perkinsus 属原虫のアサリの減耗への関与2013

    • Author(s)
      脇 司・高橋美希・悦喜達也・日浅大仁・梅田剛佑・高岸奈々絵・良永知義
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(品川キャンパス)
    • Related Report
      2012 Annual Research Report
  • [Presentation] アサリのPerkinsus属原虫の分布に影響する環境要因について2013

    • Author(s)
      梅田剛佑・高橋美希・脇 司・悦喜達也・良永知義
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(品川キャンパス)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of real-time PCR assay for discrimination and quantification of two Perkinsus spp. in Manila Clam Ruditapes philippinarum2011

    • Author(s)
      Umeda, K.・Yoshinaga, T
    • Organizer
      8th symposium of Diseases of Aqsia Aquaculture
    • Place of Presentation
      Mangalore, India
    • Year and Date
      2011-11-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Development of a real-time PCR assay for discrimination and quantification of two Perkinsus spp.in the Manila Clam Ruditapes2011

    • Author(s)
      Umeda, K, Yoshianga, T
    • Organizer
      8th Symposium of Diseases on Asain Aquaculture
    • Place of Presentation
      Mangalore,インド
    • Year and Date
      2011-11-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 野外調査アサリにおけるパーキンサス感染強度定量のためのRFTM法の標準化2011

    • Author(s)
      悦喜達也・良永知義
    • Organizer
      平成23年度日本魚病学会春季大会
    • Place of Presentation
      長崎
    • Year and Date
      2011-10-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 野外調査アサリ試料におけるパーキンサス感染強度定量のためのRFTM法の標準化2011

    • Author(s)
      悦喜達也・良永知義
    • Organizer
      平成23年度日本魚病学会秋季大会
    • Place of Presentation
      長崎大学
    • Year and Date
      2011-10-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] アサリに寄生するPerkinsus属原虫2種のReal-timePCRによる識別・定量法2011

    • Author(s)
      梅田剛佑・高橋美希・良永知義・小川和夫
    • Organizer
      平成23年度日本魚病学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] アサリに寄生するPerkinsus属2種の攻撃試験法の開発2011

    • Author(s)
      高橋美希・良永知義・小川和夫
    • Organizer
      平成23年度日本魚病学会春季大会
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] アサリに寄生する Perkinsus 属原虫2種のReal-timePCRによる識別・定量法2011

    • Author(s)
      梅田剛佑・高橋美希・良永知義・小川和夫
    • Organizer
      平成23年度日本魚病学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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