Modulation and optimization of immune response utilizing complement regulatory factors in fish
| Project/Area Number |
22380111
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Kyushu University |
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Principal Investigator |
NAKAO Miki 九州大学, 大学院・農学研究院, 教授 (50212080)
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| Co-Investigator(Kenkyū-buntansha) |
杣本 智軌 九州大学, (連合)農学研究科(研究院), 准教授 (40403993)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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| Keywords | 魚類 / 免疫 / 生体防御 / 補体 / 制御因子 / 活性化 / コイ / ギンブナ / 免疫応答 / 相互作用 / モノクローナル抗体 / 組換えタンパク質 / C3 / 補体制御因子 / SCRモジュール / 精製 / cDNAクローニング |
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| Research Abstract |
In order to elucidate molecular mechanism of complement regulatory factors, Tecrem and properdin (Pf), in modulating immune responses in fish, gene diversity, functional analysis at both mRNA and protein levels of zebrafish, carp, and ginbuna crucian carp Tecrem were conducted. We succeeded to prepare of recombinant carp Tecrem and to establish monoclonal antibody to carp Tecrem, and elucidated its wide tissue and cellular distribution, expression response to viral infection, suggesting key role of Tecrem in host immune system
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Report
(4 results)
Research Products
(41 results)
