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Modulation and optimization of immune response utilizing complement regulatory factors in fish

Research Project

Project/Area Number 22380111
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionKyushu University

Principal Investigator

NAKAO Miki  九州大学, 大学院・農学研究院, 教授 (50212080)

Co-Investigator(Kenkyū-buntansha) 杣本 智軌  九州大学, (連合)農学研究科(研究院), 准教授 (40403993)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Keywords魚類 / 免疫 / 生体防御 / 補体 / 制御因子 / 活性化 / コイ / ギンブナ / 免疫応答 / 相互作用 / モノクローナル抗体 / 組換えタンパク質 / C3 / 補体制御因子 / SCRモジュール / 精製 / cDNAクローニング
Research Abstract

In order to elucidate molecular mechanism of complement regulatory factors, Tecrem and properdin (Pf), in modulating immune responses in fish, gene diversity, functional analysis at both mRNA and protein levels of zebrafish, carp, and ginbuna crucian carp Tecrem were conducted. We succeeded to prepare of recombinant carp Tecrem and to establish monoclonal antibody to carp Tecrem, and elucidated its wide tissue and cellular distribution, expression response to viral infection, suggesting key role of Tecrem in host immune system

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (41 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 7 results) Presentation (29 results) Book (3 results)

  • [Journal Article] Identification of anti-viral cytotoxic effector cells in the ginbuna crucian carp Carassius auratus langsdorfii.2013

    • Author(s)
      Somamoto T, Nakanishi T and Nakao M.
    • Journal Title

      Dev. Comp. Immunol.

      Volume: 39 Issue: 4 Pages: 370-377

    • DOI

      10.1016/j.dci.2012.11.001

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The binding spectra of carp C3 isotypes against natural targets independent of the bindingspecificity of their thioester2012

    • Author(s)
      Ichiki S, Kato-Unoki Y, Somamoto T, Nakao M.
    • Journal Title

      Dev.Comp. Immunol.

      Volume: 38(1) Issue: 1 Pages: 10-16

    • DOI

      10.1016/j.dci.2012.03.004

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diversity of CD2 subfamily receptors in cyprinid fishes.2012

    • Author(s)
      Sameshima S, Nakao M, Somamoto T.
    • Journal Title

      Result Immunol

      Volume: 2 Pages: 25-34

    • DOI

      10.1016/j.rinim.2012.01.003

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The binding spectra of carp C3 isotypes against natural targets independent of the binding specificity of their thioester2012

    • Author(s)
      Ichiki S, Kato-Unoki Y, Somamoto T, Nakao M
    • Journal Title

      Developmental & Comparative Immunology

      Volume: (In press)(未発行)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of MHC class I and beta-2 microglobulin in a clonal strain of ginbuna crucian carp, Carassius auratus langsdorfii2011

    • Author(s)
      Urabe S, Somamoto T, Sameshima S, Unoki-Kato Y, Nakanishi T, Nakao M
    • Journal Title

      Fish Shellfish Immunol.

      Volume: 31 Issue: 3 Pages: 469-474

    • DOI

      10.1016/j.fsi.2011.06.004

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The complement system in teleost fish : Progress of post-homolog-hunting researches2011

    • Author(s)
      Nakao M, Tsujikura M, Ichiki S, Tam K.Vo, Somamoto T.
    • Journal Title

      Dev.Comp.Immunol.

      Volume: 35 Issue: 12 Pages: 1296-1308

    • DOI

      10.1016/j.dci.2011.03.003

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The complement system in teleost fish : Progress of post-homolog-hunting researches.2011

    • Author(s)
      Nakao M, Tsujikura M, Ichiki S, Tam KV, Somamoto T.
    • Journal Title

      Developmental and Comparative Immunology

      Volume: 35(in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 魚類における膜型補体制御因子の同定と機能解析:次世代魚病ワクチンアジュバントへの展開に向けて2010

    • Author(s)
      辻倉正和、杣本智軌、鵜木陽子、中尾実樹
    • Journal Title

      生物機能研究

      Volume: 14 Pages: 38-44

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 魚類における膜型補体制御因子の同定と機能解析:次世代魚病ワクチンアジュバントへの展開に向けて.

    • Author(s)
      辻倉正和、杣本智軌、鵜木陽子、中尾実樹
    • Journal Title

      生物機能研究

      Volume: 14

    • Related Report
      2012 Final Research Report
  • [Presentation] ギンブナ樹状細胞マーカーCD83 に対する抗体の作製と発現2013

    • Author(s)
      豊福太樹、山下基子、長沢貴宏、杣本智軌、中尾実樹
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京水産大学
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] コイ栓球は他の白血球により活性化され貪食能を示す2013

    • Author(s)
      長沢貴宏、中易千早、杣本智軌、中尾実樹
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京水産大学
    • Year and Date
      2013-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] コイ補体成分ProperdinのcDNAクローニングと構造解析2013

    • Author(s)
      鵜木陽子、辻倉正和、一木智子、杣本智軌, 中尾実樹.
    • Organizer
      日本水産学会九州支部大会
    • Place of Presentation
      九州大学
    • Year and Date
      2013-01-26
    • Related Report
      2012 Final Research Report
  • [Presentation] コイ栓球は他の白血球により活性化され貪食能を示す2013

    • Author(s)
      長澤貴宏
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ギンブナ樹状細胞マーカー CD83 に対する抗体の作製と発現2013

    • Author(s)
      杣本 智軌
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 穴あき病発症時におけるコイ補体成分の組織内分布2013

    • Author(s)
      前田佑佳
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Rag1欠損ゼブラフィッシュにおけるT細胞の機能不全の検証2013

    • Author(s)
      白水正道
    • Organizer
      日本水産学会九州支部大会
    • Place of Presentation
      九州大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ギンブナにおけるコイヘルペスウイルスに対すする防御機構の解明2013

    • Author(s)
      福田圭佑
    • Organizer
      日本水産学会九州支部大会
    • Place of Presentation
      九州大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] コイ膜型補体制御因子 cTecrem のモノクローナル抗体作製と機能解析2013

    • Author(s)
      中村亮太
    • Organizer
      日本水産学会九州支部大会
    • Place of Presentation
      九州大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] コイ補体成分 Properdinの cDNA クローニングと構造解析2013

    • Author(s)
      鵜木(加藤)陽子
    • Organizer
      日本水産学会九州支部大会
    • Place of Presentation
      九州大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 魚類CD46様補体制御因子の多様性と機能の解析2012

    • Author(s)
      中村亮太、原田光利、長澤貴宏、Indriyani Nur、辻倉正和、一木智子、杣本智軌、中尾実樹.
    • Organizer
      補体シンポジウム
    • Place of Presentation
      大阪府立成人病センター
    • Year and Date
      2012-08-24
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Phagocytosis and bactericidal abilities of teleost thrombocytes2012

    • Author(s)
      Nagasawa T, Nakayasu C, Matsuyama T, Rieger AM, Barreda DR, Somamoto T, NakaoM
    • Organizer
      12th Congress of International Society of Developmental and Comparative Immunology
    • Place of Presentation
      Hilton Fukuoka Sea Hawk Hotel, Fukuoka
    • Year and Date
      2012-07-11
    • Related Report
      2012 Final Research Report
  • [Presentation] Functions of CD8-positive and CD4-positive lymphocytes against virus-infection in ginbuna crucain carp2012

    • Author(s)
      Somamoto T, Nakanishi T, Nakao M.
    • Organizer
      12th Congress of International Society of Developmental and Comparative Immunology
    • Place of Presentation
      Hilton Fukuoka Sea Hawk Hotel, Fukuoka
    • Year and Date
      2012-07-10
    • Related Report
      2012 Final Research Report
  • [Presentation] ゲノムデータからみた魚類補体制御因子の多様性2012

    • Author(s)
      辻倉正和, 中尾 実樹, 杣本 智軌
    • Organizer
      アクアゲノム研究会東京大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2012-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Phagocytosis and bactericidal abilities of teleost thrombocytes2012

    • Author(s)
      Nagasawa T
    • Organizer
      12th Congress of International Society of Developmental and Comparative Immunology
    • Place of Presentation
      Fukuoka
    • Related Report
      2012 Annual Research Report
  • [Presentation] Functions of CD8-positive and CD4-positive lymphocytes against virus-infection in ginbuna crucain carp2012

    • Author(s)
      Somamoto T
    • Organizer
      12th Congress of International Society of Developmental and Comparative Immunology
    • Place of Presentation
      Fukuoka
    • Related Report
      2012 Annual Research Report
  • [Presentation] Characterization of culture supernatant of Streptococcus parauberis: hemolytic factor and leucocyte-stimulation in Japanese flounder2012

    • Author(s)
      Tsukamoto H
    • Organizer
      12th Congress of International Society of Developmental and Comparative Immunology
    • Place of Presentation
      Fukuoka
    • Related Report
      2012 Annual Research Report
  • [Presentation] Isotypic Diversity in the Ontogenetic Expression of the Complement Component in the Common Carp (Cyprinus carpio)2012

    • Author(s)
      Vo Kha Tam
    • Organizer
      12th Congress of International Society of Developmental and Comparative Immunology
    • Place of Presentation
      Fukuoka
    • Related Report
      2012 Annual Research Report
  • [Presentation] rag-1遺伝子欠損によるゼブラフィッシュの獲得免疫不全の検証と遺伝子の発現変動2011

    • Author(s)
      徳永弓枝・白水正道・安住薫・吉浦康寿・乙竹充・杣本智軌・中尾実樹
    • Organizer
      平成23年度日本水産学会秋季大会
    • Place of Presentation
      長崎大学水産学部(長崎)
    • Year and Date
      2011-09-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 補体の内外に見るホメオスタシス2011

    • Author(s)
      中尾実樹
    • Organizer
      日本動物学会第82回大会シンポジウム
    • Place of Presentation
      大雪ホール(旭川)(招待講演)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] コイ補体成分 C4 アイソタイプ(C4-1, C4-2)の結合特異性の解析2011

    • Author(s)
      山本 暁、一木智子、杣本智軌 、中尾実樹
    • Organizer
      補体シンポジウム
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2011-09-02
    • Related Report
      2012 Final Research Report
  • [Presentation] コイ補体成分C4アイソタイプ(C4-1,C4-2)の結合特異性の解析2011

    • Author(s)
      山本暁・一木智子・杣本智軌・中尾実樹
    • Organizer
      第48回補体シンポジウム
    • Place of Presentation
      名古屋市立大学(名古屋)
    • Year and Date
      2011-09-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 魚類における補体活性化制御因子の多様性2011

    • Author(s)
      辻倉正和・杣本智軌・鵜本陽子・中尾実樹
    • Organizer
      第23回日本比較免疫学会学術集会
    • Place of Presentation
      JAMSTEC(横浜)
    • Year and Date
      2011-08-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structural and functional diversity of the complement system, aninnate immune factor, in fish2011

    • Author(s)
      Nakao M, Tsujikura M, Ichiki S, Vo TK, Somamoto T
    • Organizer
      International Congress of Comparative Physiology and Biochemistry 2011
    • Place of Presentation
      名古屋国際会議場(名古屋)(招待講演)
    • Year and Date
      2011-06-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Complement system in teleost fish : Isotypic diversity in pathogenrecognition, activation cascade, and ontogeny2011

    • Author(s)
      Nakao M, Ichiki S, Tsujikura M, Vo TK, Somamoto T
    • Organizer
      Comparative Immnology and Pathology Workshop
    • Place of Presentation
      University of Alberta (Canada)(招待講演)
    • Year and Date
      2011-05-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 魚類における膜型補体制御因子の同定2010

    • Author(s)
      辻倉正和、杣本智軌、鵜木陽子、中尾実樹
    • Organizer
      第22回日本比較免疫学会学術集会
    • Place of Presentation
      福岡市
    • Year and Date
      2010-08-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functional diversity of the complement component isotypes in bony fish innate immunity: a model study using the common carp.2010

    • Author(s)
      Nakao M, Ichiki S, Mutsuro J, Tsujikura M,Somamoto T
    • Organizer
      9th International Congress on the Biology of Fish
    • Year and Date
      2010-07-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Functional diversity of the complement component isotypes in bony fish innate immunigy : A model study using the common carp2010

    • Author(s)
      Nakao M, Ichiki S, Mutsuro J, Tsujikura M, Somamoto T
    • Organizer
      The ninth International Congress on the Biology of Fish
    • Place of Presentation
      福島市(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] コイ補体レクチン経路に関連するコレクチンの異物認識多様性2010

    • Author(s)
      一木昭土、塚本春香、杣本智軌、中尾実樹
    • Organizer
      第47回補体シンポジウム
    • Place of Presentation
      福島市
    • Related Report
      2010 Annual Research Report
  • [Book] 補体への招待2011

    • Author(s)
      中尾実樹、杣本智軌
    • Publisher
      メジカルビュー
    • Related Report
      2012 Final Research Report
  • [Book] 補体への招待(2-5-2補体系の進化:脊椎動物)2011

    • Author(s)
      中尾実樹・杣本智軌
    • Total Pages
      231
    • Publisher
      メジカルビュー社
    • Related Report
      2011 Annual Research Report
  • [Book] 補体への招待「2.5.2 補体系の進化:脊椎動物」大井洋之、木下タロウ、松下操 編2011

    • Author(s)
      中尾実樹、杣本智軌
    • Total Pages
      231
    • Publisher
      Medical View
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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