Role of chemokines in immune response of teleost fish

• Project/Area Number: 22380112
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: KURATA Osamu 日本獣医生命科学大学, 獣医学部, 准教授 (90277666)
• Co-Investigator(Kenkyū-buntansha): MORITOMO Tadaaki 日本大学, 生物資源科学部, 准教授 (20239677) ; MATSUYAMA Tomomasa 独立行政法人水産総合研究センター, 研究員 (20372021) ; TAKANO Tomokazu 独立行政法人水産総合研究センター, 研究員 (40533998) ; SAKAI Takamitsu 独立行政法人水産総合研究センター, 研究員 (50416046)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: ケモカイン / IL-8 / CXCL13 / 好中球 / Bリンパ球 / 炎症 / ヒラメ / 免疫学 / 生理活性 / 細胞・組織 / 水産学 / マイクロアレイ

Research Abstract

We revealed chemotactic activity of two types of chemokines, IL-8 and CXCL13 in Japanese flounder. IL-8 and CXCL13 induced selectively the migration of neutrophils and B lymphocytes, respectively. We also produced specific antibodies to these chemokines and developed immunoassays to estimate the quantity of these chemokines. Flounder leukocytes showed various responses in their IL-8 production that induced by stimulation with several species of bacterial pathogens. We suggested that IL-8 production is relevant to diversity of immune response observed in infectious diseases of Japanese flounder.

Research Products

• [Journal Article] Clonal growth of carp (Cyprinus carpio)T cells in vitro : Long-term proliferation of Th2-like cells (2012)
  • Author(s): Yamaguchi, T., Katakura. E., Someya, K., Dijkstra, J.M., Moritomo, T., Nakanishi, T.
  • Journal Title: Fish and shellfish immunology Volume: 34 Issue: 2 Pages: 433-442
  • DOI: 10.1016/j.fsi.2012.11.005
  • Peer Reviewed
• [Journal Article] Ontogeny of anti-viral hemorrhagic septicemia virus (VHSV) immunity in developing Japaneseflounder. (2012)
  • Author(s): Matsuyama, T., Nakayasu, C., Fujiwara,A., Kurita, Takano, T., Ito, T. and Sano, M.
  • Journal Title: Dev. Comp. Immunol. Volume: 37 Issue: 3-4 Pages: 312-322
  • DOI: 10.1016/j.dci.2012.02.014
  • Peer Reviewed
• [Journal Article] Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in Japanese flounder Paralichthys olivaceus leucocytes during Edwardsiella tarda and viral hemorrhagic septicemia virus infection (2011)
  • Author(s): Matsuyatna, T., A.Fujiwara, T.Takano, C.Nakayasu
  • Journal Title: Fish & Shellfish Immunol. Volume: 31 Issue: 4 Pages: 524-532
  • DOI: 10.1016/j.fsi.2011.06.015
  • Peer Reviewed
• [Journal Article] Evaluating antigen-specific cytotoxicity of CD8+ T cells in fish by Granzyme B-like activity (2011)
  • Author(s): Toda H, Yabu T, Shiba H, Moritomo T, Nakanishi T
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 141 Issue: 1-2 Pages: 168-172
  • DOI: 10.1016/j.vetimm.2011.02.020
  • Peer Reviewed
• [Journal Article] Perforin-dependent cytotoxic mechanism in killing by CD8 positive T cells in ginbuna crucian carp, Carassius auratus langsdorfii (2011)
  • Author(s): Toda H, Araki K, Moritomo T, Nakanishi T
  • Journal Title: Developmental & Comparative Immunology Volume: 35 Issue: 1 Pages: 88-93
  • DOI: 10.1016/j.dci.2010.08.010
  • Peer Reviewed
• [Journal Article] Protective Efficacy of a Formalin-Killed Vaccine against Atypical Edwardsiella tarda Infection in Red Sea Bream Pagrus major (2011)
  • Author(s): Takano, T. , Matsuyama, T., Sakai, T.,Nakayasu, C.
  • Journal Title: Fish Pathology Volume: 46 Issue: 4 Pages: 120-122
  • DOI: 10.3147/jsfp.46.120
  • NAID: 10030255232
  • ISSN: 0388-788X, 1881-7335
  • Peer Reviewed
• [Journal Article] Detection of Japanese Flounder Antibody against Fimbrial Major Protein of Edwardsiella tarda (2011)
  • Author(s): Sakai, T., Miyoshi, Y., Matsuyama, T.,Nakayasu, C., Kamaishi, T., Fukuda, Y. and Iida, T.
  • Journal Title: Fish Pathology Volume: 46 Issue: 1 Pages: 23-26
  • DOI: 10.3147/jsfp.46.23
  • NAID: 10027836518
  • ISSN: 0388-788X, 1881-7335
  • Peer Reviewed
• [Journal Article] Lymphocytes with T-cell-like properties express the Fas ligand in the Japanese flounder Paralichthys olivaceus. (2011)
  • Author(s): Kurata, O., Iwasaki, T., Matsuyama, T.,Nakayasu, C., Wada, S. and Hatai, K.
  • Journal Title: Fish Shellfish Immunol. Volume: 30 Issue: 2 Pages: 509-514
  • DOI: 10.1016/j.fsi.2010.11.030
  • Peer Reviewed
• [Journal Article] Long-term Culture of Japanese Flounder Paralichthys olivaceus Leukocytes (2011)
  • Author(s): Ozaki, T., Hatakeyama, H., Wada, S. andKurata, O.
  • Journal Title: Fish Pathology Volume: 46 Issue: 1 Pages: 11-18
  • DOI: 10.3147/jsfp.46.11
  • NAID: 130000674859
  • ISSN: 0388-788X, 1881-7335
  • Peer Reviewed
• [Journal Article] Lymphocytes with T-cell-like properties express the Fas ligand in the Japanese flounder Paralichthys olivaceus. (2011)
  • Author(s): Osamu Kurata, Toru Iwasaki, Tomomasa Matsuyama, Chihaya Nakayasu, Shinpei Wada, Kishio Hatai
  • Journal Title: Fish and Shellfish Immunology Volume: 30 Pages: 509-514
  • Peer Reviewed
• [Journal Article] Long-term culture technology for Japanese flounder Paralichthys olivaceus leukocytes. (2011)
  • Author(s): Tomoki Ozaki, Hitoshi Hatakeyama, Shinpei Wada, Osamu Kurata
  • Journal Title: Fish Pathology Volume: 46 Pages: 11-18
  • Peer Reviewed
• [Journal Article] Detection of Japanese flounder antibody against fimbrial major protein of Edwardsiella tarda. (2011)
  • Author(s): Sakai, T., Miyoshi, Y., Matsuyama, T., Nakayasu, C., Kamaishi T., Fukuda, Y., Iida, T.
  • Journal Title: Fish Pathology Volume: 46 Pages: 23-26
  • NAID: 10027836518
  • Peer Reviewed
• [Journal Article] Activity of Granulocytes and Chemokines in the Leukocyte-encapsulation Response of Japanese Flounder Paralichthys olivaceus (2010)
  • Author(s): Kurata, O., Kitancharoen, N., Fujiwara, A., Nakayasu, C., Wada, S. and Hatai, K.
  • Journal Title: Fish Pathology Volume: 45 Issue: 3 Pages: 121-129
  • DOI: 10.3147/jsfp.45.121
  • NAID: 10026600679
  • ISSN: 0388-788X, 1881-7335
  • Peer Reviewed
• [Journal Article] Immunocytochemical studies of the ontogeny of peripheral blood leucocyte subpopulations in Japanese flounder (Paralichthys olivaceus). (2010)
  • Author(s): Matsuyama, T., Nakayasua, C. and Sano,M.
  • Journal Title: FishShellfish Immunol. Volume: 29 Issue: 2 Pages: 362-365
  • DOI: 10.1016/j.fsi.2010.03.005
  • Peer Reviewed
• [Journal Article] The efficacy of five avirulent Edwardsiella tarda strains in a live vaccine against Edwardsiellosis in Japanese flounder, Paralichthys olivaceus. (2010)
  • Author(s): Takano, T., Matsuyama, T., Oseko, N.,Sakai, T., Kamaishi, T., Nakayasu, C., Sano, M., Iida, T.
  • Journal Title: FishShellfish Immunol. Volume: 29 Issue: 4 Pages: 687-693
  • DOI: 10.1016/j.fsi.2010.07.012
  • Peer Reviewed
• [Journal Article] Demonstration of T cell and macrophage progenitors in carp (Cyprinus carpio) kidney hematopoietic tissues. (2010)
  • Author(s): Katakura, F., Yamaguchi, T., Yoshida,M., Moritomo, T. and Nakanishi, T.
  • Journal Title: Dev. Comp. Immunol. Volume: 34 Issue: 6 Pages: 685-689
  • DOI: 10.1016/j.dci.2010.01.015
  • Peer Reviewed
• [Journal Article] Clonal growth of carp (Cyprinus carpio) T cells in vitro. (2010)
  • Author(s): Yamaguchi, T., Katakura, F., Shitanda,S., Niida, Y., Toda, H., Ohtani, M., Yabu, T., Suetake, H., Moritomo, T. andNakanishi, T.
  • Journal Title: Dev. Comp. Immunol. Volume: 35 Issue: 2 Pages: 193-202
  • DOI: 10.1016/j.dci.2010.09.007
  • Peer Reviewed
• [Journal Article] Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoieticstem cells. (2010)
  • Author(s): Kobayashi, I., Ono, H., Moritomo, T.,Kano, K., Nakanishi, T. and Suda, T.
  • Journal Title: Blood Volume: 115
  • Peer Reviewed
• [Journal Article] Activity of granulocytes and chemokines in the leukocyte-encapsulation response of Japanese flounder Paralichthys olivaceus. (2010)
  • Author(s): Osamu Kurata, Nilubol Kitancharoen, Atushi Fujiwara, Chihaya Nakayasu, Shinpei Wada, Kishio Hatai
  • Journal Title: Fish Pathology Volume: 45 Pages: 121-129
  • NAID: 10026600679
  • Peer Reviewed
• [Journal Article] Immunocytochemical studies of the ontogeny of peripheral blood leucocyte subpopulations in Japanese flounder (Paralichthys olivaceus). (2010)
  • Author(s): T.Matsuyama, C.Nakayasua, M.Sano
  • Journal Title: Fish and Shellfish Immunology Volume: 29 Pages: 362-365
  • Peer Reviewed
• [Journal Article] The efficacy of five avirulent Edwardsiella tarda strains in a live vaccine against Edwardsiellosis in Japanese flounder, Paralichthys olivaceus. (2010)
  • Author(s): Takano, T., Matsuyama, T., Oseko, N., Sakai, T., Kamaishi, T., Nakayasu, C., Sano, M., Iida, T.
  • Journal Title: Fish and Shellfish Immunology Volume: 29 Pages: 687-693
  • Peer Reviewed
• [Journal Article] Demonstration of T cell and macrophage progenitors in carp (Cyprinus carpio) kidney hematopoietic tissues. (2010)
  • Author(s): Katakura, F., T.Yamaguchi, M.Yoshida, T.Moritomo, T.Nakanishi.
  • Journal Title: Developmental and Comparative Immunology Volume: 34 Pages: 685-689
  • Peer Reviewed
• [Journal Article] Clonal growth of carp (Cyprinus carpio) T cells in vitro. (2010)
  • Author(s): Yamaguchi, T., F.Katakura, S.Shitanda, Y.Niida, H.Toda, M.Ohtani, T.Yabu, H.Suetake, T.Moritomo, T.Nakanishi.
  • Journal Title: Developmental and Comparative Immunology Volume: 35 Pages: 193-202
  • Peer Reviewed
• [Journal Article] Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoietic stem cells. (2010)
  • Author(s): Kobayashi, I., H.Ono, T.Moritomo, K.Kano, T.Nakanishi, T.Suda.
  • Journal Title: Blood Volume: 115 Pages: 1-9
  • Peer Reviewed
• [Presentation] ヒラメIL-8タンパク検出のための特異抗体の作製 (2013)
  • Author(s): 倉田修
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2013-03-29
• [Presentation] 好中球の走化性に関与するヒラメIL-8のN末領域 (2012)
  • Author(s): 倉田修
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 独立行政法人水産大学校
  • Year and Date: 2012-09-15
• [Presentation] N-terminal region responsible for chemotactic activity of flounder IL-8. (2012)
  • Author(s): Kurata, O.
  • Organizer: The 12th congress of ISDCI
  • Place of Presentation: Hilton Fukuoka Sea Hawk Hotel.
  • Year and Date: 2012-07-10
• [Presentation] ヒラメCXCケモカインに対する白血球走化性について (2011)
  • Author(s): 倉田修
  • Organizer: 平成23年度日本魚病学会秋季大会
  • Place of Presentation: 長崎大学文教キャンパス
  • Year and Date: 2011-10-02
• [Presentation] 支持細胞上で増殖するヒラメ白血球の起源. (2011)
  • Author(s): 倉田修
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学文教キャンパス
  • Year and Date: 2011-10-01
• [Presentation] 支持細胞上で増殖するヒラメ白血球の起源 (2011)
  • Author(s): 倉田修・市川遼・和田新平
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学文教キャンパス
  • Year and Date: 2011-10-01
• [Presentation] Immune-related gene expression in leukocyte subpopulations of Japanese flounder. (2011)
  • Author(s): Takano, T.
  • Organizer: The 15th International Conference on Diseases of Fish and Shellfish
  • Place of Presentation: Split, Croatia.
  • Year and Date: 2011-09-14
• [Presentation] Immune-related gene expression in leukocyte subpopulations of Japanese flounder (2011)
  • Author(s): Tomokazu Takano, Tomomasa Matsuyama, Atushi Fujiwara, Takamitsu Sakai, Chihaya Nakayasu
  • Organizer: 第15回ヨーロッパ魚病学会国際大会
  • Place of Presentation: クロアチアスプリト市
  • Year and Date: 2011-09-14
• [Presentation] ヒラメCXCケモカインの機能解析. (2011)
  • Author(s): 倉田修
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2011-03-28
• [Presentation] ヒラメCXCケモカインの機能解析 (2011)
  • Author(s): 倉田修, 和田新平, 坂井貴光, 高野倫一, 松山知正
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2011-03-28
• [Presentation] 好中球の走化性に関与するヒラメIL-8のN末領域
  • Author(s): 倉田 修
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 独立行政法人水産大学校
• [Presentation] ヒラメIL-8タンパク検出のための特異抗体の作製
  • Author(s): 倉田 修
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
• [Presentation] N-terminal region responsible for chemotactic activity of flounder IL-8
  • Author(s): Osamu Kurata
  • Organizer: The 12th congress of ISDCI
  • Place of Presentation: Hilton Fukuoka Sea Hawk Hotel