| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Research Abstract |
We established the method to obtain mature eggs of the Japanese sea cucumber by incubating ripe ovaries in seawater containing 10-100nM cubifrin for 90 min. The mature eggs of >90% developed normally when immediately fertilized after the production of mature eggs. However, delayed fertilization increased the percentage of abnormally larvae because of polyspermy at fertilization and abnormal cleavages during embryogenesis.
By examining various conditions such as cryoprotectant, speed of freezing, the method for cryopreservation for sea cucumber sperm was established to obtain >80% fertilization rate.
Cross breeding between Hokkaido and Mie sea cucumber was performed by using above two methods. Morphological features of spines on the skin such as their number and height, which are economical important traits, were estimated to be genetically influenced.
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