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Novel approach to the distribution and function of globin proteins in aquatic organisms

Research Project

Project/Area Number 22380115
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTokai University (2011-2012)
The University of Tokyo (2010)

Principal Investigator

OCHIAI Yoshihiro  東海大学, 海洋学部, 教授 (50160891)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2010: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywordsグロビン類 / タンパク質 / 構造 / 機能 / 分布 / グロビン / 生理機能 / ミオグロビン / 一次構造 / 立体構造 / 分子進化 / 系統解析 / 組換え体 / 構造安定性 / 分子動力学
Research Abstract

Attempts were made to clone the genes coding globin proteins (especially myoglobin) from a variety of fish species, and based on the deduced amino acid sequences and bioinformatic analyses, the profiles of molecular evolution and environment adaptation were examined. Distribution of globins was investigated by measuring the transcription and expression levels of globin genes in various tissues of several fish species. The properties and tissue distribution of mammalian globins were also examined. On the other hand, the physiological roles of myoglobin other than oxygen molecule binding were also found.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (37 results)

All 2013 2012 2011 2010 Other

All Journal Article (15 results) (of which Peer Reviewed: 7 results) Presentation (22 results)

  • [Journal Article] Assessment of the commercial quality evaluation on yellowfin tuna Thunnus albacares meat based on the properties of myoglobin2013

    • Author(s)
      M. Nurilmala, H. Ushio, G. Kaneko, Y. Ochiai
    • Journal Title

      Food Sci. Technol. Res

      Volume: 19巻 Pages: 237-243

    • Related Report
      2012 Final Research Report
  • [Journal Article] Assessment of the Commercial Quality Evaluation on Yellowfin Tuna Thunnus albacares Meat Based on the Properties of Myoglobin2013

    • Author(s)
      M. Nurilmala, H. Ushio, G. Kaneko, Y. Ochiai
    • Journal Title

      Food Sci. Technol. Res.

      Volume: 19 Pages: 237-243

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ミオグロビン-基礎から応用まで2012

    • Author(s)
      落合芳博
    • Journal Title

      冷凍

      Volume: 87巻 Pages: 341-347

    • NAID

      10030763746

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Journal Article] Structural characterization of piscine globin superfamily proteins2012

    • Author(s)
      Y. Ochiai
    • Journal Title

      World Acad. Sci. Eng. Technol

      Volume: 66巻 Pages: 186-191

    • Related Report
      2012 Final Research Report
  • [Journal Article] Structural characterization of carangid fish myoglobins2012

    • Author(s)
      M.M. Hasan, S. Watabe, Y. Ochiai
    • Journal Title

      Fish Physiology andBiochemistry

      Volume: 38巻 Issue: 5 Pages: 1311-1322

    • DOI

      10.1007/s10695-012-9619-z

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Journal Article] Structural characterization of carangid fish myoglobins2012

    • Author(s)
      M.M. Hasan, S. Watabe, Y. Ochiai
    • Journal Title

      Fish Physiology and Biochemistry

      Volume: 38 Pages: 1311-1322

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural characterization of piscine globin superfamily proteins2012

    • Author(s)
      Y. Ochiai
    • Journal Title

      World Acad. Sci. Eng. Technol.

      Volume: 66 Pages: 186-191

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ミオグロビン―基礎から応用まで2012

    • Author(s)
      落合芳博
    • Journal Title

      冷凍

      Volume: 87 Pages: 341-347

    • NAID

      10030763746

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural perturbation of tuna myoglobin2011

    • Author(s)
      Y. Ochiai
    • Journal Title

      World Acad. Sci. Eng. Technol.

      Volume: 74巻 Pages: 178-182

    • Related Report
      2012 Final Research Report
  • [Journal Article] 冷凍条件下におけるマグロ・ミオグロビンの構造シミュレーション2011

    • Author(s)
      落合芳博
    • Journal Title

      日本冷凍空調学会論文集

      Volume: 2011 Pages: 339-342

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Temperature-dependent structural perturbation of tuna myoglobin2011

    • Author(s)
      Y.Ochiai
    • Journal Title

      World Acad.Sci.Eng.Technol

      Volume: 74 Pages: 178-182

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ワシクジラ骨格筋ミオグロビンの生化学的および熱力学的性状2010

    • Author(s)
      Y. Ochiai, Y. Watanabe, Uchida, H. Ozawa, S. Watabe
    • Journal Title

      日水誌

      Volume: 76巻 Pages: 686-694

    • Related Report
      2012 Final Research Report
  • [Journal Article] Thermal Denaturation Profiles of Tuna Myoglobin2010

    • Author(s)
      Y. Ochiai, Y. Watanabe, H. Ozawa, S. Ikegami, N. Uchida, S. Watabe
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Issue: 8 Pages: 1673-1679

    • DOI

      10.1271/bbb.100290

    • NAID

      10027558496

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Final Research Report
  • [Journal Article] Thermal denaturation profiles of tuna myoglobin2010

    • Author(s)
      Y.Ochiai, Y.Watanabe, H.Ozawa, S.Ikegami, N.Uchida, S.Watabe
    • Journal Title

      Biosci.Biotechnol.Biechem.

      Volume: 74 Pages: 1673-1679

    • NAID

      10027558496

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] イワシクジラ骨格筋ミオグロビンの生化学的および熱力学的性状2010

    • Author(s)
      落合芳博, 渡辺良明, 内田直行, 小澤秀夫, 渡部終五
    • Journal Title

      日水誌

      Volume: 76 Pages: 686-694

    • NAID

      10026550956

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Widespread expression of myoglobin in muscle and non-muscle tissues of hypoxia-intolerant species,rainbow trout2013

    • Author(s)
      M.M. Hasan, G. Kaneko, H. Ushio, S. Watabe, Y. Ochiai
    • Organizer
      Aquatic Sciences Meeting 2013
    • Place of Presentation
      Convention Center, New Orleans, USA
    • Year and Date
      2013-02-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Phylogeny of globin superfamily: diversification and distribution of myoglobin in teleosts2012

    • Author(s)
      M. M. Hasan, G. Kaneko, H. Ushio, S. Watabe, Y. Ochiai
    • Organizer
      日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2012-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Structural characterization and tissue-specific expression of disk abalone Haliotis discus Indoleamine dioxygenase-like myoglobin2012

    • Author(s)
      M.M. Hasan, T. Sasaki, H. Ushio, S. Watabe, Y. Ochiai
    • Organizer
      The 9th Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      カルポート(高知)
    • Year and Date
      2012-07-13
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Fast preparation and autoxidation profile of tuna myoglobin2012

    • Author(s)
      M. Nurilmala, S. Watabe, Y. Ochiai
    • Organizer
      日本水産学 会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2012-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Tissue-specific expression of the transcripts encoding the rainbow trout myoglobin gene2012

    • Author(s)
      Hasan Muhammad Mehedi, Shugo Watabe, Yoshihiro Ochiai
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Fast preparation and autooxidation profile of tuna myoglobin2012

    • Author(s)
      Mala Nurilmala, Shugo Watabe, Yoshihiro Ochiai
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] マグロ・ミオグロビンの温度依存的な構造ゆらぎ2012

    • Author(s)
      落合芳博
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structural and evolutionary characterization of myoglobin from southern bluefin tuna Thunnus maccoyii2011

    • Author(s)
      Y. Ochiai
    • Organizer
      International Symposium on Muscle Biochemistry
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-10-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Uniqueness of myoglobins from aquatic animals: their structures and stabilities2011

    • Author(s)
      M. Nurilmala, S. Watabe, Y. Ochiai
    • Organizer
      Internatioanl Symposium on Muscle Biochemistry
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-10-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Uniqueness of myoglobins from aquatic animals : their structures and stabilities2011

    • Author(s)
      Yoshihiro Ochiai
    • Organizer
      International Symposium on Muscle Biochemistry
    • Place of Presentation
      東京大学農学部(東京)
    • Year and Date
      2011-10-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structural profiles and tissue-specific gene transcription of rainbow trout Onchorhynchus mykiss myoglobin2011

    • Author(s)
      Hasan Muhammad Mehedi, Shugo Watabe, Yoshihiro Ochiai
    • Organizer
      International Symposium on Muscle Biochemistry
    • Place of Presentation
      東京大学農学部(東京)
    • Year and Date
      2011-10-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structural and evolutionary characterization of myoglobin from southern bluefin tuna Thunnus maccoyii2011

    • Author(s)
      Mala Nurilmala, Shugo Watabe, Yoshihiro Ochiai
    • Organizer
      International Symposium on Muscle Biochemistry
    • Place of Presentation
      東京大学農学部(東京)
    • Year and Date
      2011-10-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 水圏生物グロビン類の構造特性2011

    • Author(s)
      落合芳博
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      長崎大学水産学部(長崎)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Evolutionary and structural aspects of fish myoglobins2011

    • Author(s)
      Yoshihiro Ochiai
    • Organizer
      8th International Congress on Comparative Biochemistry and Physiology
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-06-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Evolutionary and structural aspects of fish myoglobins2011

    • Author(s)
      Y. Ochiai
    • Organizer
      International Congress of Comaparative Physiology and Biochemistry
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-06-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Structural profiles of southern bluefin tuna Thunnus maccoyii myoglobin2011

    • Author(s)
      M.Nurilmala, S.Watabe, Y.Ochiai
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cloning, characterization, tissue distribution and gene expression of myoglobin from rainbow trout Onchorhynchus mykiss2011

    • Author(s)
      M.M.Hasan, S.Watabe, Y.Ochiai
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Regional stability differences in fish myoglobin2010

    • Author(s)
      落合芳博
    • Organizer
      日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural decay of fish myoglobin through thermal treatment2010

    • Author(s)
      Y. Ochiai
    • Organizer
      OzBIO
    • Place of Presentation
      Melbourne Convention and Exhibition Center (Austlaria)
    • Year and Date
      2010-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Structural decay of fish myoglohin through thermal treatment2010

    • Author(s)
      Y.Ochiai
    • Organizer
      OzBio2010
    • Place of Presentation
      オーストラリア、メルボルン
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Widespread expression of myoglobin in muscle and non-muscle tissues of hypoxia-intolerant species, rainbow trout

    • Author(s)
      M.M. Hasan, G. Kaneko, H. Ushio, S. Watabe, Y. Ochiai
    • Organizer
      Aquatic Sciences Meeting 2013
    • Place of Presentation
      Convention Center, New Orleans, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Phylogeny of globin superfamily: diversification and distribution of myoglobin in teleosts

    • Author(s)
      M. M. Hasan, G. Kaneko, H. Ushio, S. Watabe, Y. Ochiai
    • Organizer
      日本生化学会大会
    • Place of Presentation
      国際会議場(福岡)
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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