Construction of anti-ulcerative colitis (UC) yogurt by probiotic blood type lactic acid bacteria

• Project/Area Number: 22380144
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Tohoku University
• Principal Investigator: SAITO Tadao 東北大学, 大学院・農学研究科, 教授 (00118358)
• Co-Investigator(Kenkyū-buntansha): KITAZAWA Haruki 東北大学, 大学院・農学研究科, 准教授 (10204885) ; KAWAI Yasushi 東北大学, 大学院・農学研究科, 助教 (00261496) ; NISHIMURA Junko 東北大学, 大学院・農学研究科, 技術専門職員 (10241556)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: 乳酸菌 / プロバイオティクス / 血液型乳酸菌 / 潰瘍性大腸炎 / 競合阻害 / ビフィ ズス菌 / アドヘシン / 機能性ヨーグルト / ビフィズス菌 / 経腸栄養剤 / 潰瘍性大腸菌

Research Abstract

A new evaluation system to select probiotic lactic acid bacteria (LAB) with adherence to human colonic mucin (HCM) by recognizing sialic acid and/or surfate on ABO-blood type antigens. LAB strains showing strong adhesion to human blood type-A antigen expressed on the intestinal mucosa were selected from 237 probiotic strains using the biosensor BIACORE. A similar method was used to select harmful bacteria that also recognizing the same antigen in the human intestine. Fusobacterium varium was detected as one of harmful bacteria from the patient of ulcerative colitics. Using a competitive adhesion experiment with a micro plate coated with the type-A antigen and the harmful bacteria, the bacteria was significantly decreased when they were added in combination with the type-A LAB. Our data suggested the blood type LAB strains may competitively inhibit pathogenic infections where this strategy may be useful in the development of improved future probiotic foods to protect human health.

Research Products

• [Journal Article] New screening methods for probiotics with adhesion properties to sialic acid and sulfate residues in human colonic mucin using the Biacore assay. (2013)
  • Author(s): Huang I-N, Okawara, T, Watanabe M, Kawai Y, Kitazawa H, Ohnuma S, Shibata C, Horii A, Kimura K, Taketomo N, Xiao J, Iwatsuki K, Saito T.
  • Journal Title: J Appl Microbiol Volume: 114 Issue: 3 Pages: 854-860
  • DOI: 10.1111/jam.12063
  • Peer Reviewed
• [Journal Article] Food preservative potential of gassericin A-containing concetrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA39. (2013)
  • Author(s): K.Nakamura
  • Journal Title: Anim. Sci. J. Volume: 84 Issue: 2 Pages: 144-149
  • DOI: 10.1111/j.1740-0929.2012.01048.x
  • Peer Reviewed
• [Journal Article] Chemical characterisation of oligosacchairdes in commerciallly pasteurised dromedary camel (Camelus dromedarius) milk. (2013)
  • Author(s): O.A.Alhaj
  • Journal Title: Int. Dairy J. Volume: 28 Issue: 2 Pages: 70-75
  • DOI: 10.1016/j.idairyj.2012.08.008
  • Peer Reviewed
• [Journal Article] プロバイオティクスの評価システム構築と製品化への新展開 (2013)
  • Author(s): 齋藤 忠夫
  • Journal Title: 食品と開発 Volume: ４８ Pages: 8-11
  • Peer Reviewed
• [Journal Article] Characterization of lactose utilization and beta-galactosidase in Lactobacillus brevis KB290, thehetero-fermentative lactic acid bacterium (2012)
  • Author(s): Honda, H., N.Yajima and T. Saito
  • Journal Title: Curr. Microbiol Volume: 65 Issue: 6 Pages: 679-685
  • DOI: 10.1007/s00284-012-0216-2
  • Peer Reviewed
• [Journal Article] Purification and characterization of two phosphor-beta -galactosidases, LacG1 and LacG2, from Lactobacillus gasseri ATCC33323T (2012)
  • Author(s): Honda, H., S. Nagaoka, Y. Kawai, R.Kemperman, J. Kok, Y. Yamazaki, Y. Tateno, H. Kitazawa and T. Saito
  • Journal Title: J. Gen. Appl. Microbiol Volume: 58 Pages: 11-17
  • Peer Reviewed
• [Journal Article] An Adhesin-Like Protein, Lam29, from <i>Lactobacillus mucosae</i> ME-340 Binds to Histone H3 and Blood Group Antigens in Human Colonic Mucus (2012)
  • Author(s): Watanabe M, Kinoshita H, Huang IN, Eguchi K, Tsurumi T, Kawai Y, Kitazawa H, Kimura K, Taketomo N, Kikuchi D, Sase T, Miura K, Ogawa H, Shibata C, Horii A, Saito T.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 9 Pages: 1655-1660
  • DOI: 10.1271/bbb.120150
  • NAID: 130004137955
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] 「乳酸菌」プロバイオティッ ク乳酸菌のヒト腸管付着性機構の解明と応用 (2012)
  • Author(s): 齋藤忠夫
  • Journal Title: New Diet Therapy Volume: 27 Pages: 13-22
• [Journal Article] プロバイオティクスの評価システム構築と製品化への新展開 (2012)
  • Author(s): 齋藤忠夫
  • Journal Title: 食品と開発 Volume: 48 Pages: 8-11
• [Journal Article] New screening methods for probiotics with adhesion properties to sialic acid and sulphate residues in human colonic mucin using the Biacore assay. (2012)
  • Author(s): I-N.Huang
  • Journal Title: J. Appl. Microbiol. Volume: 114 Pages: 854-860
  • Peer Reviewed
• [Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway (2012)
  • Author(s): Shimazu, T., H.Aso, Y.Suda, Y.Kawai, T.Saito, H.Kitazawa, et al
  • Journal Title: Infect.Immun. Volume: 80 Issue: 1 Pages: 276-288
  • DOI: 10.1128/iai.05729-11
  • Peer Reviewed
• [Journal Article] 「乳酸菌」プロバイオティック乳酸菌のヒト腸管付着性機構の解明と応用 (2012)
  • Author(s): 齋藤 忠夫
  • Journal Title: 日本臨床栄養協会誌 Volume: 27 Pages: 13-22
  • Peer Reviewed
• [Journal Article] Purification and characterization of two phospho-β-galactosidases, LacG1 and LacG2, from Lactobacillus gasseri ATCC33323T. (2012)
  • Author(s): H. Honda
  • Journal Title: J. Gen. Appl. Microbiol. Volume: 58 Pages: 11-17
  • Peer Reviewed
• [Journal Article] Purification and characterization of two phosphor-beta-galactosidases, LacG1 and LacG2, from Lactobaciulls gasserin ATCC33323 (2012)
  • Author(s): H.Honda, et al
  • Journal Title: J.Gen.Appl.Microbiol. Volume: 58 Pages: 11-17
  • Peer Reviewed
• [Journal Article] 細菌における細胞分裂メカニズムと乳酸菌の形態変化について (2011)
  • Author(s): 西村順子、齋藤忠夫
  • Journal Title: Milk Science Volume: 60 Pages: 111-117
  • NAID: 130003398704
  • Peer Reviewed
• [Journal Article] プロバイオティック乳酸菌のヒト腸管付着性機構の解明と応用 (2011)
  • Author(s): 齋藤忠夫
  • Journal Title: New Diet Therapy Volume: 27 Pages: 13-22
  • Peer Reviewed
• [Journal Article] Oral administration of phosphorylated dextran regulates immune response in ovalbumin- immunized mice (2010)
  • Author(s): Nagasawa, C., Nishimura-Uemura, J., Tohono, M., T. Shimosato, Y. Kawai, S. Ikegami, M. Oda, T. Saito and H.Kitazawa
  • Journal Title: Asian-Aust. J. Anim. Sci Volume: 23 Pages: 106-115
  • Peer Reviewed
• [Journal Article] Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens (2010)
  • Author(s): M. Watanabe, H. Kinoshita, M. Nitta, R. Yukishita, Y. Kawai, K. Kimura, N. Taketomo, Y. Yamazaki, Y. Tateno, K. Miura, A. Horii, H. Kitazawa and T. Saito
  • Journal Title: Journal of applied Microbiology Volume: 109巻 Issue: 3 Pages: 927-935
  • DOI: 10.1111/j.1365-2672.2010.04719.x
  • Peer Reviewed
• [Journal Article] Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigen (2010)
  • Author(s): M.Watanabe, et al
  • Journal Title: J.Appl.Microbiol. Volume: 109 Pages: 927-935
  • Peer Reviewed
• [Journal Article] Oral administration of Phosphorylated dextran regulates immune response in ovalbumin-immunized mice (2010)
  • Author(s): C.Nagasawa, et al
  • Journal Title: Asian-Aust.J.Anim.Sci. Volume: 23 Pages: 106-115
  • Peer Reviewed
• [Presentation] 二成分性バクテリオシン「ガセリシンT」の連結発現およびC末端アミノ酸残基の欠損が抗菌効果に及ぼす影響 (2013)
  • Author(s): 水谷 陽（齋藤忠夫）
  • Organizer: 日本畜産学会
  • Place of Presentation: 安田女子大学キャンパス（広島）
• [Presentation] ヒト腸疾患の予防につながる大腸ムチン酸性基を介したヒト腸管付着性プロバイオティクスの新規選抜方法の開発と応用 (2013)
  • Author(s): 黄 懿儂（齋藤 忠夫）
  • Organizer: 日本畜産学会
  • Place of Presentation: 安田女子大学キャンパス（広島）
• [Presentation] Lactic Acid Bacteria and Dairy Foods, Inaugural Worldwide Symposium on Milk and Human Health (2012)
  • Author(s): Saito, T
  • Organizer: Organized by Korean Society for Lactic Acid Bacteria, Shilla Hotel, Seoul
  • Place of Presentation: KOREA
  • Year and Date: 2012-11-08
• [Presentation] Immunogenics and survival strategy of Lactobacillus GG in human gut (2012)
  • Author(s): Saito, T
  • Organizer: The 3rd International Symposium on Lactobacillus GG of the Korean Society for Lactic Acid Bacteria (KSLAB), Grand Ballroom, The Hotel Plaza Seoul
  • Place of Presentation: Seoul City, Korea
  • Year and Date: 2012-05-09
• [Presentation] New Functional Yogurts as FOSHU and Their Evaluation System in Japan (2012)
  • Author(s): Saito, T
  • Organizer: The 7th International Symposium on Lactic Acid Bacteria and Health , The 3rd Asian Symposium on Lactic Acid Bacteria
  • Place of Presentation: Wuxi, China
• [Presentation] New evaluation system using Biacore to select probiotics with high binding activity to acidic residues of human colonic mucin (2012)
  • Author(s): Huang, I. N., T. Okawara, M. Watanabe, Y. Kawai, H. Kitazawa, S. Ohnuma, C. Shibata, A. Horii, T. Saito
  • Organizer: The 7th International Symposium on Lactic Acid Bacteria and Health, The 3rd Asian Symposium on Lactic Acid Bacteria
  • Place of Presentation: Wuxi, China
• [Presentation] 潰瘍性大腸炎の炎症緩和効果を有するプロバイオティクスに関する基盤的研究(その1) (2012)
  • Author(s): 佐藤優理、坂本光央、小川仁、三浦康、柴田近、川井泰、北澤春樹、大熊盛也、堀井明、齋藤忠夫
  • Organizer: 日本農芸化学会2012年度大会、京都女子大学
  • Place of Presentation: 京都
• [Presentation] 潰瘍性大腸炎(UC)の炎症緩和効果を示す血液型乳酸菌に関する基盤的研究(その1) (2012)
  • Author(s): 齋藤忠夫、佐藤優理、坂本光央、大熊盛也、小川仁、三浦康、柴田近、川井泰、北澤春樹、堀井明
  • Organizer: 日本畜産学会第115回大会、名古屋大学
  • Place of Presentation: 名古屋
• [Presentation] Lactobacillus mucosae由来アドへシン様タンパク質(Lam29)の新規ヒト大腸粘液由来結合因子の探索および同定 (2012)
  • Author(s): 渡辺真通、木下英樹、川井泰、北澤春樹、三浦康、柴田近、堀井明、木村勝紀、竹友直生、齋藤忠夫
  • Organizer: 日本畜産学会第115回大会、名古屋大学
  • Place of Presentation: 名古屋
• [Presentation] プロバイオティック乳酸菌のヒト大腸ムチンにおけるシアル酸・硫酸基結合評価系のBiacoreによる新構築 (2012)
  • Author(s): 黄懿儂、春田美由樹、渡辺真通、江口慧、鶴見卓也、大川原崇、川井泰、北澤春樹、大沼忍、柴田近、堀井明、木村勝紀、竹友直生、齋藤忠夫
  • Organizer: 日本畜産学会第115回大会、名古屋大学
  • Place of Presentation: 名古屋
• [Presentation] ヒト腸内細菌の大腸ムチン結合部位決定のための酸性基に注目した新しいBiacore評価系の構築 (2012)
  • Author(s): 黄懿儂、渡辺真通、大川原崇、川井泰、北澤春樹、大沼忍、柴田近、堀井明、木村勝紀、竹友直生、齋藤忠夫
  • Organizer: 日本乳酸菌学会2012年度大会、つくば国際会議場
  • Place of Presentation: つくば
• [Presentation] The recent study of lactic acid bacteria and application to functional yogurt in Japan. (2012)
  • Author(s): Tadao Saito
  • Organizer: 2012 International Conference on Fermentation -Lactic acid bacteria for healty living-
  • Place of Presentation: Business Hall (B2B), Jeonju Worldcup Stadium, Jeonju, Jeonbuk, KOREA
  • Invited
• [Presentation] New functional yogurts as FOSHU and their evaluation system in Japan (2012)
  • Author(s): Tadao Saito
  • Organizer: The 7th Internationa Symposium on Lactic Acid Bacteria and Health. The 3rd Asiacn Symposium on Lactic Acid Bacteria.
  • Place of Presentation: 山明水秀大飯店、無錫、中国
  • Invited
• [Presentation] Future Strategy of new yogurts using adhesive probiotic lactic acid bacteria (LAB) to protect our gut from inflammatory bowel disease (IBD) (2011)
  • Author(s): T.Saito
  • Organizer: The 6^<th> Asian Conference on Lactic Acid Bacteria
  • Place of Presentation: Sappro
  • Year and Date: 2011-09-10
• [Presentation] New prevention strategy for inflammatory bowel disease using probiotic lactic acid bacteria and/or Bifidobacterium Yogurt (2011)
  • Author(s): T.Saito
  • Organizer: 2011 Annual Meeting of Korean Society of Food Science and Technology
  • Place of Presentation: Daegu Exhibition Convention Center (EXCO), Korea
  • Year and Date: 2011-06-08
• [Presentation] New Prevention Strategy for Inflammatory Bowel Disease Using Probiotic Lactic Acid Bacteria and/or Bifidobacterium Yogurt. Product Development Division-Food Microbiology Division. Application of Functional Microorganisms inFermented Food. "Food Biosciences: Pomises & Challenges in the Future" (2011)
  • Author(s): Saito, T
  • Organizer: 2011 Annual Meeting of Korean Society of Food Science and Technology (KoSFoST)
  • Place of Presentation: EXCO, Daegu, Korea
• [Presentation] Future Strategy of New Yogurts Using Adhesive Probiotic Lactic Acid Bacteria (LAB) to Protect Our Gut from Inflammatory Bowel Disease (IBD). LAB6-6, Session6, Technologies and Applications (2011)
  • Author(s): Saito, T
  • Organizer: The 6th Asian Conference on Lactic Acid Bacteria. XII International Congress of Bacteriology and Applied Microbiology (ACLAB6), Organized by Japan Society for Lactic Acid Bacteria (JSLAB) and Asian Federation of Societies for Lactic Acid Bacteria (AFSLAB), Sapporo Convention Center & Sapporo Business Innovation Center
  • Place of Presentation: Sapporo, Japan
• [Presentation] New prevention strategy for inflammatory bowel disease using probiotic lactic acid bacteria and/or Bifidobacterium with specific adhesion to human intestine (2011)
  • Author(s): Saito, T., R. Yukishita, M. Watanabe, H. Kitazawa, Y. Kawai, A. Horii, F. Ikezawa, A. Yamamura, H. Ogawa, K. Miura, C. Shibata, I. Sasaki
  • Organizer: 10th Symposium on Lactic Acid Bacteria
  • Place of Presentation: Egmond aan Zee, Netherland
• [Presentation] Lactobacillus mucosae由来ABCトランスポータータンパク質(Lam29)のヒト大腸粘液中の新規受容体様分子の同定 (2011)
  • Author(s): 渡辺真通、江口慧、鶴見卓也、川井泰、北澤春樹、齋藤忠夫、山村明寛、生澤史江、三浦康、小川仁、佐々木巌、堀井明、木村勝紀、竹友直生
  • Organizer: 日本乳酸菌学会2011年度大会、関西大学
  • Place of Presentation: 大阪
• [Presentation] Strategy for New Japanese Yogurts using Functional Probiotic Lactic Acid Bacteria (LAB) (2010)
  • Author(s): Tadao Saito
  • Organizer: 14^<th> AAAP Animal Science Congress
  • Place of Presentation: Pingtung, Taiwan, ROC
  • Year and Date: 2010-08-23
• [Presentation] Immunogenics and survial strategy of Lactobacillu GG in human gut.
  • Author(s): Tadao Saito
  • Organizer: 2012 The 3rd Internationa Symposium on Lactobacillus GG of the Korean Society for Lactic Acid Bacteria.
  • Place of Presentation: Grand Ballroom, The Hotel Plaza Seoul. Korea
  • Invited
• [Book] Lactic Acid Bacteria and Bifidobacteria (Current Progress in Advanced Research) (Ed.by K.Sonomoto, A.Yokota) (2011)
  • Author(s): Y.Kawai, T.Saito
  • Publisher: Caister Academic Press (Norfolk, UK)
• [Book] 畜産物利用学(齋藤忠夫、根岸晴夫、八田一編) (2011)
  • Author(s): 齋藤忠夫
  • Publisher: 文永堂出版(東京)
• [Book] 乳酸菌とビフィズス菌のサイエンス (2010)
  • Author(s): 齋藤忠夫
  • Total Pages: 668
  • Publisher: 京都大学学術出版会
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/douka/index-j.html