Construction of anti-ulcerative colitis (UC) yogurt by probiotic blood type lactic acid bacteria
| Project/Area Number |
22380144
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Tohoku University |
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Principal Investigator |
SAITO Tadao 東北大学, 大学院・農学研究科, 教授 (00118358)
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| Co-Investigator(Kenkyū-buntansha) |
KITAZAWA Haruki 東北大学, 大学院・農学研究科, 准教授 (10204885)
KAWAI Yasushi 東北大学, 大学院・農学研究科, 助教 (00261496)
NISHIMURA Junko 東北大学, 大学院・農学研究科, 技術専門職員 (10241556)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | 乳酸菌 / プロバイオティクス / 血液型乳酸菌 / 潰瘍性大腸炎 / 競合阻害 / ビフィ ズス菌 / アドヘシン / 機能性ヨーグルト / ビフィズス菌 / 経腸栄養剤 / 潰瘍性大腸菌 |
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| Research Abstract |
A new evaluation system to select probiotic lactic acid bacteria (LAB) with adherence to human colonic mucin (HCM) by recognizing sialic acid and/or surfate on ABO-blood type antigens. LAB strains showing strong adhesion to human blood type-A antigen expressed on the intestinal mucosa were selected from 237 probiotic strains using the biosensor BIACORE. A similar method was used to select harmful bacteria that also recognizing the same antigen in the human intestine. Fusobacterium varium was detected as one of harmful bacteria from the patient of ulcerative colitics. Using a competitive adhesion experiment with a micro plate coated with the type-A antigen and the harmful bacteria, the bacteria was significantly decreased when they were added in combination with the type-A LAB. Our data suggested the blood type LAB strains may competitively inhibit pathogenic infections where this strategy may be useful in the development of improved future probiotic foods to protect human health.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] New screening methods for probiotics with adhesion properties to sialic acid and sulfate residues in human colonic mucin using the Biacore assay.2013
Author(s)
Huang I-N, Okawara, T, Watanabe M, Kawai Y, Kitazawa H, Ohnuma S, Shibata C, Horii A, Kimura K, Taketomo N, Xiao J, Iwatsuki K, Saito T.
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Journal Title
J Appl Microbiol
Volume: 114
Issue: 3
Pages: 854-860
DOI
Related Report
Peer Reviewed
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[Journal Article] Purification and characterization of two phosphor-beta -galactosidases, LacG1 and LacG2, from Lactobacillus gasseri ATCC33323T2012
Author(s)
Honda, H., S. Nagaoka, Y. Kawai, R.Kemperman, J. Kok, Y. Yamazaki, Y. Tateno, H. Kitazawa and T. Saito
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Journal Title
J. Gen. Appl. Microbiol
Volume: 58
Pages: 11-17
Related Report
Peer Reviewed
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[Journal Article] An Adhesin-Like Protein, Lam29, from <i>Lactobacillus mucosae</i> ME-340 Binds to Histone H3 and Blood Group Antigens in Human Colonic Mucus2012
Author(s)
Watanabe M, Kinoshita H, Huang IN, Eguchi K, Tsurumi T, Kawai Y, Kitazawa H, Kimura K, Taketomo N, Kikuchi D, Sase T, Miura K, Ogawa H, Shibata C, Horii A, Saito T.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 76
Issue: 9
Pages: 1655-1660
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Journal Article] Oral administration of phosphorylated dextran regulates immune response in ovalbumin- immunized mice2010
Author(s)
Nagasawa, C., Nishimura-Uemura, J., Tohono, M., T. Shimosato, Y. Kawai, S. Ikegami, M. Oda, T. Saito and H.Kitazawa
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Journal Title
Asian-Aust. J. Anim. Sci
Volume: 23
Pages: 106-115
Related Report
Peer Reviewed
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[Journal Article] Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens2010
Author(s)
M. Watanabe, H. Kinoshita, M. Nitta, R. Yukishita, Y. Kawai, K. Kimura, N. Taketomo, Y. Yamazaki, Y. Tateno, K. Miura, A. Horii, H. Kitazawa and T. Saito
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Journal Title
Journal of applied Microbiology
Volume: 109巻
Issue: 3
Pages: 927-935
DOI
Related Report
Peer Reviewed
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[Presentation] New evaluation system using Biacore to select probiotics with high binding activity to acidic residues of human colonic mucin2012
Author(s)
Huang, I. N., T. Okawara, M. Watanabe, Y. Kawai, H. Kitazawa, S. Ohnuma, C. Shibata, A. Horii, T. Saito
Organizer
The 7th International Symposium on Lactic Acid Bacteria and Health, The 3rd Asian Symposium on Lactic Acid Bacteria
Place of Presentation
Wuxi, China
Related Report
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[Presentation] New prevention strategy for inflammatory bowel disease using probiotic lactic acid bacteria and/or Bifidobacterium with specific adhesion to human intestine2011
Author(s)
Saito, T., R. Yukishita, M. Watanabe, H. Kitazawa, Y. Kawai, A. Horii, F. Ikezawa, A. Yamamura, H. Ogawa, K. Miura, C. Shibata, I. Sasaki
Organizer
10th Symposium on Lactic Acid Bacteria
Place of Presentation
Egmond aan Zee, Netherland
Related Report
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