Molecular dynamics to restore/remodel neuromuscular connections during muscle growth and regeneration
| Project/Area Number |
22380145
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Kyushu University |
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Principal Investigator |
TATSUMI Ryuichi 九州大学, 大学院・農学研究院, 准教授 (40250493)
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| Co-Investigator(Kenkyū-buntansha) |
FURUSE Mitsuhiro 九州大学, 大学院・農学研究院, 教授 (30209176)
MIZUNOYA Wataru 九州大学, 大学院・農学研究院, 助教 (20404056)
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| Research Collaborator |
RONALD E Allen 米国アリゾナ大学, 教授
JUDY E Anderson 加国マニトバ大学, 理学部, 教授
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | 食肉 / 骨格筋 / 筋幹細胞 / 運動神経 / 神経軸索成長ガイダンス因子Sema3A / 肝細胞増殖因子(HGF) / 塩基性線維芽細胞増殖因子(FGF2) / トランスフォーミング増殖因子(TGF)-β / 畜産学 / 神経軸索成長ガイダンス因子 / Sema3A / 細胞増殖因子 / 神経軸索ガイダンス因子 / 神経細胞軸索ガイダンス因子 |
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| Research Abstract |
Successful regeneration and remodeling of the neuromuscular connections are critical for restoring functional properties of contractile physiology of muscle fibers. While the spatiotemporal regulatory mechanisms coordinating these processes with myogenesis itself remain unclear, various neural factors including attractive and repulsive axon-guidance cue ligands and their receptors may be involved. Recently, we found that satellite cells, resident myogenic stem cells normally positioned beneath the basal lamina of mature muscle fibers, up-regulate a secreted neural chemorepellent semaphorin 3A (Sema3A) during early-differentiation period, upon acute muscle injury in vivo(Tatsumi et al., 2009). The current study encouraged the possible regulation of Sema3A expression by time-coordinated prevalence of extracellular up-regulator (HGF and FGF2) and down-regulator (TGF-β3) concentrations bathing differentiating satellite cells (myoblasts) in regenerating muscle tissue. By understanding this growth factor ligand-driving scenario, we will be able to design new procedures that specifically target regenerative motoneuritogenesis to ensure muscle growth and repair, contributing to the meat-animal production and human sports and health sciences aimed to enhance physical performance and medical therapies for neuromuscular disorders and age-related muscle atrophy
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Report
(4 results)
Research Products
(77 results)
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[Journal Article] Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle2012
Author(s)
Do, M.-K. Q., Suzuki, T., Gerelt, B., Sato, Y., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Tatsumi, R
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Journal Title
Animal Science Journal
Volume: 83
Pages: 712-717
Related Report
Peer Reviewed
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[Journal Article] Growth factor regulation of neural chemorepellent Sema3A expression in satellite cell cultures2011
Author(s)
Do, M.-K. Q., Sato, Y., Shimizu, N., Suzuki, T., Shono, J.-I., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Tatsumi, R
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Journal Title
American Journal of Physiology-Cell Physiology
Volume: 301
Issue: 5
Pages: C1270-C1279
DOI
Related Report
Peer Reviewed
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[Journal Article] In vitromeasurement of postnatal changes in proliferating satellite cell frequency during rat muscle growth2011
Author(s)
Suzuki, T., Takaishi, H., Sakata, T., Do, M. Q., Hara, M., Sato, A., Mizunoya, W., Nishimura, T, Hattori, A., Ikeuchi, Y., and Tatsumi, R
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Journal Title
Animal Science Journal
Volume: 81
Issue: 2
Pages: 245-251
DOI
Related Report
Peer Reviewed
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[Presentation] Heparan/chondroitin sulfate chains may mediate HGF/FGF2-induced up-regulation of neural chemorepellent Sema3A in satellite cell cultures2012
Author(s)
Do, M.Q., Shimizu, N., Suzuki, T., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J.E.,and Tatsumi, R
Organizer
2012 FASEB Science Research Conference on "Skeletal Muscle Satellite & Stem Cells"
Place of Presentation
Barga, Lucca, Italy
Related Report
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[Presentation] differentiation of satellite cells2012
Author(s)
Suzuki, T., Ojima, K., Do, M.Q., Hara, M., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J.E., and Tatsumi, R
Organizer
2012 FASEB Science Research Conference on "Skeletal Muscle Satellite & Stem Cells"
Place of Presentation
Barga, Lucca, Italy
Related Report
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[Presentation] Mechano-sensing calcium-influx machinery that instigates skeletal muscle satellite cell activation2012
Author(s)
Tatsumi, R., Hara, M., Tabata, K., Suzuki, T., Do, M.-K. Q., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Allen, R. E
Organizer
2012 FASEB Science Research Conference on "Skeletal Muscle Satellite & Stem Cells"
Place of Presentation
Barga, Lucca, Italy
Related Report
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[Presentation] Mechano-biology of resident myogenic stem cells: implication in postnatal myogenesis and intramuscular motoneuritogenesis2010
Author(s)
Tatsumi, R.,Mizunoya, W., Ikeuchi, Y., Hattori, A., Anderson, J. E., and Allen, R. E
Organizer
Animal Science Congress 2010 of the Asian-Australian Association of Animal Production Societies (AAAP)
Place of Presentation
National Pingtung University of Science and Technology (NPUST), Pingtung, Taiwan
Related Report
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[Presentation] Possible implication of satellite cells in regenerative motoneuritogenesis: HGF and FGF2 upregulate neural chemorepellent Sema3A expression2010
Author(s)
Tatsumi, R.,Do, M.-K. Q., Shimizu, N., Sankoda, Y., Anderson, J. E., Sato, Y., Suzuki, T., Mizunoya, W., Ikeuchi, Y., and Allen, R. E.
Organizer
the 6thWorld Congress on Biomechanics (WCB) 2010, in conjunction with the 14thInternational Conference on Biomedical Engineering (ICBME) 2010 and the 5thAsian Pacific Conference on Biomechanics (APBiomech) 2010
Place of Presentation
Suntec Convention and Exhibition Center, Singapore
Related Report
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[Presentation] Possible implication of satellite cells in regenerative motoneuritogenesis: temporal coordination of HGF/FGF-2/TGF-beta may regulate neural chemorepellent Sema3A expression2010
Author(s)
Do, M.-K. Q., Shimizu, N., Sato, Y., Suzuki, T., Tatsumi, R.,Mizunoya, W., Ikeuchi, Y., Anderson, J. E., and Allen, R. E
Organizer
2010 FASEB Summer Research Conference on "Skeletal Muscle Satellite & Stem Cells"
Place of Presentation
Carefree, AZ, US
Related Report
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