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Establishment of animal production system with reduced use of antimicrobials by profiling the specific gravity of white blood cell

Research Project

Project/Area Number 22380146
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionKyoto Prefectural University

Principal Investigator

USHIDA Kazunari  京都府立大学, 大学院・生命環境科学研究科, 教授 (50183017)

Co-Investigator(Kenkyū-buntansha) INOUE Ryo  京都府立大学, 大学院・生命環境科学研究科, 講師 (70443926)
TSUKAHARA Takamitsu  京都府立大学, 大学院・生命環境科学研究科, 特任講師 (90562091)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords減投薬家畜生産 / 不顕感染 / 健康診断システム / 白血球比重 / 末梢血白血球比重
Research Abstract

Removal or reduced use of antimicrobials in integrated animal production is a current issue for the safe animal production policy. We have developed a rapid test for in-apparent infection of piglets and calves using specific gravities of white blood cells. When the relationship between change in specific gravity of white blood cells and the scores for presence of pathogens in blood, or swabs of feces and pharynx, tested with piglets at various stages, a significant negative correlation was observed. In particular, change in blood cells of 1.072-1.084g/ml was evident. Granulocytes were the major components of this particular fraction of white blood cells, therefore, percentages of the granulocytes in that fraction was measured. There was a significant negative correlation between percentages of this specific granulocyte and scores for presence of respiratory pathogens. This substantiates the possibility of the present methodology for the rapid test of in-apparent respiratory infection in piglets. In case of calves, tested animals were all raised in experimental farms and numbers of animals were limited. The detection of relationships between the specific gravities of white blood cells and in-apparent infection was not observed due to the relative clean environment to commercial pig production facilities. Further studies are required to confirm the reliability of the methodology in calves.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (9 results)

All 2013 2012 2011 2010 Other

All Journal Article (2 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Brachyspira属細菌の養豚野外試料からの検出2011

    • Author(s)
      牛田一成・朴鐘吉・塚原隆充・伊藤貢・中西信夫
    • Journal Title

      Brachyspira

      Volume: 2(1) Pages: 18-27

    • Related Report
      2012 Final Research Report
  • [Journal Article] 自然界における薬剤耐性菌汚染-環境の視点から2010

    • Author(s)
      牛田一成
    • Journal Title

      臨床と微生物(近代出版)

      Volume: 36巻6号 Pages: 623-628

    • Related Report
      2012 Final Research Report
  • [Presentation] 初生からの仔ブタ腸管免疫発達と離乳時期が免疫発達に及ぼす影響2013

    • Author(s)
      中谷麻紗子・塚原隆充・井上亮・福田菊人・牛田一成
    • Organizer
      日本畜産学会第116回大会
    • Place of Presentation
      安田女子大学
    • Year and Date
      2013-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 免疫学、栄養学的および遺伝子発現という観点から仔ブタの離乳時期を考える2012

    • Author(s)
      井上亮
    • Organizer
      日本畜産学会第115回大会(招待講演)
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 遺伝学的系統の異なる豚繁殖障害・呼吸障害症候群ウイルスに対する細胞性獲得免疫の応答2012

    • Author(s)
      原山智子・井上亮・塚原隆充・牛田一成
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-03-28
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 免疫学、栄養学的および遺伝子発現という観点から仔ブタの離乳時期を考える2012

    • Author(s)
      井上亮
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋大学(招待講演)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 仔豚の免疫担当細胞の分画と哺乳期における経日的変化2011

    • Author(s)
      原山智子・井上亮・塚原隆充・福田菊人・牛田一成
    • Organizer
      日本畜産学会第114回大会
    • Place of Presentation
      北里大学
    • Year and Date
      2011-08-26
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 初生からの仔ブタ腸管免疫発達と離乳時期が免疫発達に及ぼす影響

    • Author(s)
      中谷麻紗子・塚原隆充・井上亮・福田菊人・牛田一成
    • Organizer
      日本畜産学会
    • Place of Presentation
      安田女子大学
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://seika.kpu.ac.jp/~k_ushida/ushidaintro.html

    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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