Grant-in-Aid for Scientific Research (B)
A long-term culture of male primitive germ cells from bovine testis has been examined. Bovine primitive germ cells can proliferate and survive in culture for over 2 months in the condition with serum-free components and on poly-L-lysine-coated dish. The resulting cell colonies have both pluripotent and stem cell potent characteristics. After culture of cells in the presence of growth factors, cells are differentiated into spermatogonia.
Reprod Fertil Dev
Volume: in press
Cell Stem Cells
Journal of Reproduction and Development
The Proceedings for 16th World Congress on In Vitro Fertilization
European Journal of Wildllife Research
Proc Natl Acad Sci U S A.
Embryonic Stem Cells-Differentiation and Pluripotent Alternatives(ed.Michael S.Kallos, InTech Publisher, Rijeka)
Proceedings of the National Academy of Science USA
Genes to Cells
European Journal of Wild Animal Research
Attribution of KAKENHI