Dissection ofvarious ovarian functions, and extraction and reconstruction of oogenesis-specific functions to develop oocyte growth culture systems for domestic animals
| Project/Area Number |
22380151
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kobe University |
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Principal Investigator |
MIYANO Takashi 神戸大学, 大学院・農学研究科, 教授 (80200195)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAO Yuji 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所, 上席研究員 (10355349)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | 卵母細胞 / 卵巣 / 卵胞 / 体外培養 |
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| Research Abstract |
Bovine and porcine primordial follicles did not develop beyond the primary stage in culture. Bovine secondary follicles develop in the bovine plasma-supplemented medium, and the oocytes (approximately 60 μm in diameter) grew to 90 μm or more after 4 weeks. The medium containing a high concentration of polyvinylpyrrolidone, estradiol-17β and androstenedione supported the growth and acquisition of meiotic competence of bovine growing oocytes (90-100 μm in diameter) isolated from early antral follicles. Porcine growing oocytes from early antral follicles grew to the final size and acquired the meiotic competence in the medium containing FSH, dbcAMP and estradiol-17β.
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Report
(4 results)
Research Products
(63 results)
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[Presentation] 未熟卵,卵胞の体外培養2012
Author(s)
平尾雄二
Organizer
第30回日本受精着床学会学術講演会
Place of Presentation
リーガロイヤルホテル大阪 (大阪市)
Year and Date
2012-08-30
Related Report
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[Presentation] Nuclei and nucleoli in pig oocytes.2011
Author(s)
Miyano T, Ogushi S, Kyogoku H, Fulka JJr.
Organizer
The 8th Annual Meeting of Asian Reproductive Biotechnology Society
Place of Presentation
Lijiang Waterfall Hotel(桂林, 中国)
Year and Date
2011-10-26
Related Report
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[Presentation] Nuclei and nucleoli in pig oocytes2011
Author(s)
Miyano T, Ogushi S, Kyogoku H, Fulka JJr
Organizer
The 8th Annual Meeting of Asian Reproductive Biotechnology Society
Place of Presentation
Lijiang Waterfall Hotel(中国,桂林)(招待講演)
Year and Date
2011-10-26
Related Report
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[Presentation] 未熟卵，卵胞の体外培養
Author(s)
平尾雄二
Organizer
第30回日本受精着床学会学術講演会
Place of Presentation
リーガロイヤルホテル大阪 （大阪市）
Related Report
Invited
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