Elucidation of the interaction beween Babesia parasites and host erythrocytes

• Project/Area Number: 22380154
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: YOKOYAMA Naoaki 帯広畜産大学, 原虫病研究センター, 教授 (80301802)
• Co-Investigator(Kenkyū-buntansha): 五十嵐 郁男 国立大学法人帯広畜産大学, 原虫病研究センター, 教授 (80159582)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: バベシア / 赤血球 / 寄生 / 制御性T細胞 / 免疫回避 / アクチン / スペリカルボディ / マイクロネーム / 膜 / cytochrome P450 / 遺伝子多型 / マクロファージ / Gliding motility / Spherical body protein / 原虫 / メロゾイト / 赤内型ステージ / バベシア原虫 / 膜分子 / 寄生戦略 / Na,K-ATPase

Research Abstract

This research project had been conducted to elucidate the interaction between Babesia parasites and host erythrocytes during their asexual growth. The findings are summarized below.
1) Na, K-ATPase, an erythrocytic enzyme, is found on the surface of Babesia parasite. 2) A spherical body protein (SBP) expressed by Babesia parasite is released into the cytoplasm of the infected erythrocytes at the late phase of infection. 3) Micronemal proteins of Babesia parasites act as adhesion molecules to the receptors of host erythrocytes. 4) A surface antigen of Babesia parasite can interact with the erythrocytic actin during the infection. 5) Babesia parasite shows a unique "Gliding motility" during erythrocyte invasion. 6) Babesia parasite can escape from host immunity by activating host Treg cells. 7) Erythrocyte invasion of Babesia parasites can be inhibited by disturbing the molecular interactions between the protozoan ligands and host receptors.

Research Products

• [Journal Article] A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries (2013)
  • Author(s): Yoshinari, T., Sivakumar, T., Asada, M., Battsetseg, B., Huang, X., Lan, D. T. B., Inpankaew, T., Ybanez, A. P., Alhassan, A., Thekisoe, O. M. M., de macido, A. C., Inokuma, H., Igarashi, I., and Yokoyama, N
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 2 Pages: 211-214
  • DOI: 10.1292/jvms.12-0329
  • NAID: 130001879670
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia. (2013)
  • Author(s): Munkhjargal T, Sivakumar T, Battsetseg B, Nyamjargal T, Aboulaila M, et al.
  • Journal Title: Infection, Genetics and Evolution Volume: 16 Pages: 178-185
  • DOI: 10.1016/j.meegid.2013.02.005
  • Peer Reviewed
• [Journal Article] Molecular survey of bovine vector-borne pathogens in Cebu (2013)
  • Author(s): Ybanez, A.P., Sivakumar, T., Ybanez, R.H., Vincoy, M.R., Tingson, J.A., Perez, Z.O., Gabotero, S.R., Buchorno, L.P., Inoue, N., Matsumoto, K., Inokuma, H., and Yokoyama, N
  • Journal Title: Philippines. Vet. Parasitol Volume: 196 Issue: 1-2 Pages: 13-20
  • DOI: 10.1016/j.vetpar.2013.02.013
  • Peer Reviewed
• [Journal Article] Genetic diversity of merozoite surface antigens in Babesia bovis detected from Sri Lankan cattle (2013)
  • Author(s): Sivakumar, T., Okubo, K., Igarashi, I., de Silva, W.K., Kothalawala, H., Silva, S.S., Vimalakumar, S.C., Meewewa, A.S., and Yokoyama, N
  • Journal Title: Infect. Genet. Evol Volume: 19 Pages: 134-40
  • DOI: 10.1016/j.meegid.2013.07.001
  • Peer Reviewed
• [Journal Article] The Genetic Diversity of Merozoite Surface Antigen 1 (MSA-1) among <i>Babesia bovis</i> Detected from Cattle Populations in Thailand, Brazil and Ghana (2013)
  • Author(s): Nagano, D., Sivakumar, T., de DE Macedo, A.C., Inpankaew, T., Alhassan, A., Igarashi, I., and Yokoyama, N
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 11 Pages: 1463-1470
  • DOI: 10.1292/jvms.13-0251
  • NAID: 130003362252
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam (2013)
  • Author(s): Sivakumar, T., Lan, D.T., Long, P.T., Yoshinari, T., Tattiyapong, M., Guswanto, A., Okubo, K., Igarashi, I., Inoue, N., Xuan, X., and Yokoyama, N
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 11 Pages: 1455-1462
  • DOI: 10.1292/jvms.13-0221
  • NAID: 130003362251
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] The molecular prevalence and MSA-2b gene-based genetic diversity of Babesia bovis in dairy cattle in Thailand (2013)
  • Author(s): Simking, P., Saengow, S., Bangphoomi, K., Sarataphan, N., Wongnarkpet, S., Inpankaew, T., Jittapalapong, S., Munkhjargal, T., Sivakumar, T., Yokoyama, N., and Igarashi, I
  • Journal Title: Vet. Parasitol Volume: 197 Issue: 3-4 Pages: 642-8
  • DOI: 10.1016/j.vetpar.2013.07.015
  • Peer Reviewed
• [Journal Article] Molecular Characterization of a New Babesia bovis Thrombospondin-Related Anonymous Protein (BbTRAP2) (2013)
  • Author(s): Terkawi, M.A., Ratthanophart, J., Salama, A., Aboulaila, M., Asada, M., Ueno, A., Alhasan, H., Guswanto, A., Masatani, T., Yokoyama, N., Nishikawa, Y., Xuan, X., and Igarashi, I
  • Journal Title: PLoS One Volume: 8 Issue: 12 Pages: e83305-e83305
  • DOI: 10.1371/journal.pone.0083305
  • Peer Reviewed
• [Journal Article] Specific antibody to a conserved region of Babesia apical membrane antigen-1 inhibited the invasion of B. bovis into the erythrocyte (2013)
  • Author(s): Salama, A. A., Terkawi, M. A., Kawai, S., AbouLaila, M., Nayel, M., Mousa, A., Zaghawa, A., Yokoyama, N., and Igarashi, I
  • Journal Title: Exp Parasitol Volume: 135 Issue: 3 Pages: 623-8
  • DOI: 10.1016/j.exppara.2013.09.017
  • Peer Reviewed
• [Journal Article] Babesia ovata in cattle : A review (2013)
  • Author(s): Sivakumar, T., and Yokoyama, N
  • Journal Title: Jpn J. Vet. Parasitol Volume: 12 Pages: 11-20
  • Peer Reviewed
• [Journal Article] In vitro inhibitory effect of gedunin on Babesia and Theileria parasites (2013)
  • Author(s): Azirwan, G., Thillaiampalam, S., Muncharee, T., Yokoyama, N., and Igarashi, I
  • Journal Title: J. Protozool. Res Volume: 23 Pages: 1-6
  • NAID: 120006399378
  • URL: http://www.obihiro.ac.jp/~protozoa/journal.html
  • Peer Reviewed
• [Journal Article] Evaluation of in vitro and in vivo inhibitory effects of fusidic acid on Babesia and Theileria parasites. (2012)
  • Author(s): Salama AA, Aboulaila M, Moussa AA, et al.
  • Journal Title: Veterinary Parasitology Volume: 191 Issue: 1-2 Pages: 1-10
  • DOI: 10.1016/j.vetpar.2012.08.022
  • Peer Reviewed
• [Journal Article] Farsenyl pyrophosphate synthase is a potential molecular drug target of risedronate in Babesia bovis. (2012)
  • Author(s): Ueno A, Terkawi MA, Yokoyama M, Cao S, Aboge G, Aboulaila M, et al.
  • Journal Title: Parasitology International Volume: 28 Issue: 2 Pages: 189-192
  • DOI: 10.1016/j.parint.2012.12.005
  • Peer Reviewed
• [Journal Article] Downregulation of Hepatic Cytochrome P450 3A in Mice Infected with Babesia microti (2012)
  • Author(s): Shimamoto, Y., Sasaki, M., Ikadai, H., Ishizuka, M., Yokoyama, N., Igarashi, I., Hoshi, F., Kitamura, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 2 Pages: 241-245
  • DOI: 10.1292/jvms.11-0036
  • NAID: 130001190494
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Phylogenetic relationships of Mongolian Babesia bovis isolates based on the merozoite surface antigen (MSA)-1, MSA-2b, and MSA-2c genes (2012)
  • Author(s): Altangerel, K., Sivakumar, T., Battsetseg, B., Battur, B., Ueno, A., Igarashi, I., and Yokoyama, N
  • Journal Title: Vet. Parasitol Volume: 184 Issue: 2-4 Pages: 309-316
  • DOI: 10.1016/j.vetpar.2011.09.021
  • Peer Reviewed
• [Journal Article] Macrophages are critical for cross-protective immunity conferred by Babesia microti against Babesia rodhaini infection in mice (2012)
  • Author(s): Li, et al
  • Journal Title: Infect.Immun. Volume: 80 Issue: 1 Pages: 311-320
  • DOI: 10.1128/iai.05900-11
  • Peer Reviewed
• [Journal Article] Stable expression of green fluorescent protein and targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis with the WR99210/dhfr selection system (2012)
  • Author(s): Asada, M., Tanaka, M., Goto, Y., Yokoyama, N., Inoue, N, and Kawazu, S
  • Journal Title: Mol. Biochem. Parasitol Volume: 181 Issue: 2 Pages: 162-170
  • DOI: 10.1016/j.molbiopara.2011.11.001
  • Peer Reviewed
• [Journal Article] Molecular and serological prevalence of Babesia bovis and Babesia bigemina in cattle from central region of Syria (2012)
  • Author(s): Terkawi, M., A., Alhasan, H., Huyen, N. X., Sabagh, A., Awier. K., Cao, S., Goo, Y.-K., Aboge, G., Yokoyama, N., Nishikawa, Y., Kalb-Allouz, A. K., Tabbaa, D., Igarashi, I., and Xuan, X
  • Journal Title: Vet. Parasitol Volume: 187 Issue: 1-2 Pages: 307-11
  • DOI: 10.1016/j.vetpar.2011.12.038
  • Peer Reviewed
• [Journal Article] Genetic detection of Babesia bigemina from Mongolian cattle using apical membrane antigen-1 gene-based PCR assay. (2012)
  • Author(s): Sivakumar T, Altangerel K, Battsetseg B, Battur B, Aboulaila M, et al.
  • Journal Title: Veterinary Parasitology Volume: 187 Issue: 1-2 Pages: 17-22
  • DOI: 10.1016/j.vetpar.2012.01.008
  • Peer Reviewed
• [Journal Article] Antibacterials targeting the apicoplast inhibit the growth of Babesia parasites (2012)
  • Author(s): AbouLaila.M., Munkhjargal, T., Sivakumar, T., Ueno, A., et al
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 56 Issue: 6 Pages: 3196-3206
  • DOI: 10.1128/aac.05488-11
  • Peer Reviewed
• [Journal Article] PCR Detection of Babesia ovata from Cattle Reared in Japan and Clinical Significance of Co-Infection with Theileria orientalis (2012)
  • Author(s): Sivakumar, T., Tagawa, M., Yoshinari, T., Ybanez, A. P., Igarashi, I., Ikehara, Y., Hata, H., Kondo, S., Matsumoto, K., Inokuma, H., and Yokoyama, N
  • Journal Title: J. Clin. Microbiol Volume: 50 Issue: 6 Pages: 2111-3
  • DOI: 10.1128/jcm.00220-12
  • Peer Reviewed
• [Journal Article] C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses (2012)
  • Author(s): Terkawi MA, Alhasan H, Ueno A, Ratthanophart J, Luo Y, Cao S, Kamvingkird K, Aboulaila M, et al.
  • Journal Title: Parasitology International Volume: 61 Issue: 3 Pages: 493-496
  • DOI: 10.1016/j.parint.2012.02.006
  • Peer Reviewed
• [Journal Article] Gliding Motility of Babesia bovis Merozoites Visualized by Time-Lapse Video Microscopy (2012)
  • Author(s): Asada, M., Goto, Y., Yahata, K., Yokoyama, N., Kawai, S., Inoue, N., Kaneko, O., and Kawazu, S.-I
  • Journal Title: PLoS ONE Volume: 7 Issue: 4 Pages: e35227-e35227
  • DOI: 10.1371/journal.pone.0035227
  • Peer Reviewed
• [Journal Article] Inhibitory Effects of Pepstatin A and Mefloquine on the Growth of Babesia Parasites. (2012)
  • Author(s): Munkhjargal T, Aboulaila M, Terkawi MA, Sivakumar T., et al.
  • Journal Title: American Journal of Tropical Medicine and Hygiene Volume: 87 Issue: 4 Pages: 681-688
  • DOI: 10.4269/ajtmh.2012.12-0218
  • Peer Reviewed
• [Journal Article] Expression and immunological characterization of the heat shock protein-70 homologue from Babesia bigemina. (2012)
  • Author(s): Aboulaila M, Terkawi MA, Seuseu FJ, Ota N, et al.
  • Journal Title: Veterinary Parasitology Volume: 190 Issue: 3-4 Pages: 401-410
  • DOI: 10.1016/j.vetpar.2012.06.035
  • Peer Reviewed
• [Journal Article] Cloning and characterization of histone deacetylase from Babesia bovis. (2012)
  • Author(s): Munkhjargal T, Aboulaila M, Ueno A, Sivakumar T, et al.
  • Journal Title: Veterinary Parasitology Volume: 190 Issue: 3-4 Pages: 423-433
  • DOI: 10.1016/j.vetpar.2012.06.026
  • Peer Reviewed
• [Journal Article] A PCR-based survey of selected Babesia and Theileria parasites in cattle in Sri Lanka (2012)
  • Author(s): Sivakumar, T., Kothalawala, H., Abeyratne, S. A., Vimalakumar, S. C., Meewewa, A. S., Hadirampela, D. T., Puvirajan, T., Sukumar, S., Kuleswarakumar, K., Chandrasiri, A. D., Igarashi, I., and Yokoyama N
  • Journal Title: Vet. Parasitol Volume: 190 Issue: 1-2 Pages: 263-7
  • DOI: 10.1016/j.vetpar.2012.05.014
  • Peer Reviewed
• [Journal Article] Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand (2012)
  • Author(s): Cao, et al.
  • Journal Title: Parasitology Research Volume: 111 Issue: 3 Pages: 1259-1266
  • DOI: 10.1007/s00436-012-2960-4
  • Peer Reviewed
• [Journal Article] Serum of Babesia rodhaini Infected Mice down Regulates Catalase Activity of Healthy Erythrocyte (2012)
  • Author(s): Chiou, S.-P., Yokoyama, N., Igarashi, I., Kitoh, K., and Takashima, Y
  • Journal Title: Exp Parasitol Volume: 132 Issue: 3 Pages: 327-33
  • DOI: 10.1016/j.exppara.2012.08.004
  • Peer Reviewed
• [Journal Article] Theileria equi merozoite antigen-2 interacts with actin molecule of equine erythrocyte during their asexual development (2012)
  • Author(s): Kumar, S., Yokoyama, N., Kim, J.-Y., Bork-Mimm, S., Inoue, N., Xuan, X., Igarashi, I., and Sugimoto, C
  • Journal Title: Exp Parasitol Volume: 132 Issue: 4 Pages: 508-12
  • DOI: 10.1016/j.exppara.2012.09.013
  • Peer Reviewed
• [Journal Article] Spherical body protein 4 is a new serological antigen for the global detection of Babesia bovis infection in cattle (2011)
  • Author(s): Terkawi, M. A., Huyen, N. X., Wibowo, P. E., Seuseu, F. J., Aboulaila, M., Ueno, A., Goo, Y.-K., Yokoyama, N., Xuan, X., and Igarashi, I
  • Journal Title: Clin Vaccine. Immunol Volume: 18 Issue: 2 Pages: 337-342
  • DOI: 10.1128/cvi.00388-10
  • Peer Reviewed
• [Journal Article] Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the northeast region of Thailand (2011)
  • Author(s): Terkawi, M. A., Huyen, N. X., Shinuo, C., Inpankaew, T., Maklon, K., Aboulaila, M., Ueno, A., Goo, Y. K., Yokoyama, N., Jittapalapong, S., Xuan, X., and Igarashi, I
  • Journal Title: Vet. Parasitol Volume: 178 Issue: 3-4 Pages: 201-207
  • DOI: 10.1016/j.vetpar.2011.01.041
  • Peer Reviewed
• [Journal Article] Secretion of a new spherical body protein of Babesia bovis into the cytoplasm of infected erythrocytes (2011)
  • Author(s): Terkawi, M. A., Seuseu, F. J., Eko-Wibowo, P., Huyen, N. X , Minoda, Y., Aboulaila, M., Kawai, S., Yokoyama, N., Xuan, X., and Igarashi, I
  • Journal Title: Mol. Biochem Parasitol Volume: 178 Issue: 1-2 Pages: 40-45
  • DOI: 10.1016/j.molbiopara.2011.02.006
  • Peer Reviewed
• [Journal Article] Serological survey of Babesia bovis and Babesia bigemina in cattle in South Africa (2011)
  • Author(s): Terkawi, M. A., Thekisoe, O. M. M., Katsande, C., Latif, A. A., Mansb, B. J., Matthee, O., Mkize, N., Mabogoane, N., Marais, F., Yokoyama, N., Xuan, X., and Igarashi, I
  • Journal Title: Vet. Parasitol Volume: 182 Issue: 2-4 Pages: 337-342
  • DOI: 10.1016/j.vetpar.2011.05.047
  • Peer Reviewed
• [Journal Article] In vitro growth inhibitory effect of (-)-Epigallocatechin-3-gallate from green tea on the growth of equine Babesia parasites (2011)
  • Author(s): AbouLaila, M., Terkawi, M. A., Yokoyama, N., and Igarashi, I
  • Journal Title: J. Protozool. Res Volume: 21 Pages: 30-35
  • URL: http://www.obihiro.ac.jp/~protozoa/journal.html
  • Peer Reviewed
• [Journal Article] In vitro growth inhibitory effect of (-)-Epigallocatechin-3-gallate from green tea on the growth of equine Babesia parasites. (2011)
  • Author(s): AbouLaila, M.
  • Journal Title: J. Protozool. Res. Volume: 21 Pages: 30-35
  • Peer Reviewed
• [Journal Article] Evaluation of the in vitro growth-inhibitory effect of epoxomicin on Babesia parasites (2010)
  • Author(s): AbouLaila. M., Nakamura, K., Govind, Y., Yokoyama, N., and Igarashi, I
  • Journal Title: Vet. Parasitol Volume: 167 Issue: 1 Pages: 19-27
  • DOI: 10.1016/j.vetpar.2009.09.049
  • Peer Reviewed
• [Journal Article] Development and evaluation of a nested PCR based on spherical body protein 2 gene for the diagnosis of Babesia bovis (2010)
  • Author(s): AbouLaila. M., Yokoyama, N., and Igarashi, I
  • Journal Title: Vet. Parasitol Volume: 169 Issue: 1-2 Pages: 45-50
  • DOI: 10.1016/j.vetpar.2009.12.013
  • Peer Reviewed
• [Journal Article] Inhibitory effect of terpene nerolidol on the growth of Babesia parasites (2010)
  • Author(s): AbouLaila, M., Sivakumar, T, Yokoyama, N., and Igarashi, I
  • Journal Title: Parasitol Volume: 59(Int) Issue: 2 Pages: 278-282
  • DOI: 10.1016/j.parint.2010.02.006
  • NAID: 10029824547
  • Peer Reviewed
• [Journal Article] Seroprevalence of Babesia infections of dairy cows in northern Thailand (2010)
  • Author(s): Iseki, H., Zhou, L., Kim, C., Inpankaew, T., Sununta, C., Yokoyama, N., Xuan, X., Jittapalapong, S., and Igarashi, I
  • Journal Title: Vet. Parasitol Volume: 170 Issue: 3-4 Pages: 193-196
  • DOI: 10.1016/j.vetpar.2010.02.038
  • Peer Reviewed
• [Journal Article] Development and evaluation of two nested PCR assays for the detection of Babesia bovis from cattle blood (2010)
  • Author(s): Aboulaila, M., Yokoyama, N., and Igarashi, I
  • Journal Title: Vet. Parasitol Volume: 172 Issue: 1-2 Pages: 65-70
  • DOI: 10.1016/j.vetpar.2010.04.011
  • Peer Reviewed
• [Journal Article] Differences in CD4^+CD25^+ regulatory T cell-depletion between Babesia microti and Babesia rodhaini infections in mice (2010)
  • Author(s): Yokoyama, N., Ikehara, Y., Tiwananthagorn. W., Ota, N., Igarashi. I., Kojima, N., Taguchi, O., and Tsujimura, K
  • Journal Title: J. Protozool. Res Volume: 20 Pages: 20-30
  • URL: http://jglobal.jst.go.jp/public/20090422/201102212717857027
  • Peer Reviewed
• [Journal Article] Differences in CD4^+CD25^+ regulatory T cell-depletion between Babesia microti and Babesia rodhaini infections in mice (2010)
  • Author(s): Yokoyama, N., Ikehara, Y., Tiwananthagorn. W., Ota, N., Igarashi. I., Kojima, N., Taguchi, O., Tsujimura, K.
  • Journal Title: J.Protozool.Res. Volume: 20 Pages: 4-6
  • Peer Reviewed
• [Journal Article] Molecular characterizations of a new spherical body protein of Babesia bovis and evaluation its potential use for serodiagnosis (2010)
  • Author(s): Terkawi, M.A., Seuseu, J., AbouLaila, M., Yokoyama, N., Xuan, X., Iearashi, I.
  • Journal Title: Jpn.J.Vet.Parasitol. Volume: 8 Pages: 145-145
• [Journal Article] Apical membrane antgen-1 (AMA-1) : New aspects towards babesial vaccine candidate (2010)
  • Author(s): Terkawi, M.A., AbouLaila, M., Yokoyama, N., Xuan, X., Igarashi, I.
  • Journal Title: Jpn.J.Vet.Parasitol. Volume: 9 Pages: 73-73
• [Presentation] Inhibitory effect of allicin on the growth of Babesia and Theileria equi parasites (2013)
  • Author(s): Salama, A., AbouLaila, M., Terkawi, M., Ahmed, M., Ahmed, E., Allaam, M.,横山直明、五十嵐郁男
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] Babesia bovisチオレドキシンペルオキシターゼBbTPx-1ノックアウト原虫は活性窒素種負荷に対する感受性が上昇する (2013)
  • Author(s): 麻田正仁、Carlos, S.,薄井美帆、後藤康之、横山直明、井上昇、矢幡一英、金子修、河津信一郎
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] Discovery of a new Babesia bovis thrombospondin-related anonymous protein as a novel blood-stage vaccine candidate (2013)
  • Author(s): 五十嵐郁男、Terkawi, M.A.,横山直明
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] バイオイメージングによるBabesia bovisメロゾイト滑走運動の研究 (2013)
  • Author(s): 麻田正仁、後藤康之、矢幡一英、横山直明、井上昇、金子修、河津信一郎
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] Molecular epidemiology and clinical significance of Theileria orientalis and Babesia ovate in cattle (2013)
  • Author(s): Sivakumar, T.,横山直明、五十嵐郁男
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学
• [Presentation] MSA-1 gene-based genetic diversity of Babesia bovis detected from cattle populations in Thailand, Brazil, and Ghana (2013)
  • Author(s): 横山直明、長野太輔、Sivakumar, T.,五十嵐郁男
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学
• [Presentation] Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia (2013)
  • Author(s): 五十嵐郁男、Munkhjargal, T., Sivakumar, T.,横山直明
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学
• [Presentation] Molecular survey of bovine vector-borne pathogens in Cebu, Philippines (2013)
  • Author(s): Yabanez, A.P., Sivakumar, T.,井上昇、松本高太郎、猪熊壽、横山直明
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学
• [Presentation] MSA-1 gene-based genetic diversity of Babesia bovis detected in cattle populations in Thailand, Brazil, and Ghana. (2013)
  • Author(s): 横山直明、長野大輔、T. Sivakumar、五十嵐郁男
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学
• [Presentation] Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia. (2013)
  • Author(s): 五十嵐郁男、T. Munkhjargal、T. Sivakumar、横山直明
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学
• [Presentation] Epidemiology of bovine Babesia and Theileria parasites in Sri Lanka (2012)
  • Author(s): Sivakumar, T.,五十嵐郁男、横山直明
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
• [Presentation] Validating M17 leucine aminopeptidase of Babesia parasite as a molecular drug target (2012)
  • Author(s): Aboge, G.O.,曹世諾、Terkawi, T.,正谷達謄、具潤景、横山直明、五十嵐郁男、西川義文、鈴木宏志、玄学南
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
• [Presentation] H_2O_2蛍光プローブを用いたバベシア原虫細胞内酸化ストレスの可視化 (2012)
  • Author(s): 麻田正仁、薄井美帆、後藤康之、横山直明、井上昇、河津信一郎
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
• [Presentation] Expression and immunological characterization of the heat shock protein-70 homologue from Babesia bigemina (2012)
  • Author(s): Aboulaila, M., Terkawi, M., Fassoa, J., Ohta, N., Caine, A., Yokoyama, N., Xuan, X., and Igarashi, I
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
• [Presentation] Evaluation of in vitro inhibitory effect of fusidic acid on Babesia parasites (2012)
  • Author(s): Salama, A., AbouLaila, M., Mousa, A., Nayel, M.A., El-Sify, A., Terkawi, M.A., Hassan, H.Y., Yokoyama, N., and Igarashi, I
  • Organizer: 第53回日本熱帯医学会大会
  • Place of Presentation: とかちプラザ
• [Presentation] Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia (2012)
  • Author(s): Munkhjargal, T., Sivakumar, T., Battsetseg, B., Nyamjargal, T., Purevtseren, B., Bayarsaikhan, D., Battur, B., Byambaa, B., AbouLaila, M., Terkawi, M.A., Yokoyama, N., and Igarashi, I
  • Organizer: 第53回日本熱帯医学会大会
  • Place of Presentation: とかちプラザ
• [Presentation] Characterization of Babesia bigemina heat shock protein-70 (2012)
  • Author(s): AbouLaila, M., Terkawi, M.A., Susso, J.F., Ohta, N., deMacido, A.C., Yokoyama, N., and Igarashi, I
  • Organizer: 第53回日本熱帯医学会大会
  • Place of Presentation: とかちプラザ
• [Presentation] Phylogenetic relationships of Mongolian Babesia bovis isolates based on the merozoite surface antigen (MSA)-1, MSA-2b, and MSA-2c genes (2012)
  • Author(s): 横山直明、Altangerel, K., Sivakumar, T., Battsetseg, B., Battur, B.,上野晃生、五十嵐郁男
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 大宮ソニックシティ
• [Presentation] PCR detection of Babesia ovata from cattle reared in Japan and clinical significance of co-infection with Theileria orientalis (2012)
  • Author(s): Sivakumar, T.,田川道人、吉成健志、五十嵐郁男、池原譲、秦寛、近藤誠司、松本高太郎、猪熊壽、横山直明
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 大宮ソニックシティ
• [Presentation] Evaluation of a diagnositic PCR assay for the global detection of Babesia ovate in cattle (2012)
  • Author(s): 吉成健志、Sivakumar, T.,玄学南、五十嵐郁男、横山直明
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 大宮ソニックシティ
• [Presentation] バベシア原虫における遺伝子操作技術の確立とバイオイメージングへの応用 (2012)
  • Author(s): 麻田正仁、田中みほ、菅沼啓輔、後藤康之、川合覚、横山直明、井上昇、河津信一郎
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 大宮ソニックシティ
• [Presentation] Epidemiology of bovine Babesia and Theileria parasites in Sri Lanka (2012)
  • Author(s): T. Sivakumar、五十嵐郁男、横山直明
  • Organizer: 第154回日本獣医学会
  • Place of Presentation: 岩手大学
• [Presentation] H2O2蛍光プローブを用いたバベシア原虫細胞内酸化ストレスの可視化 (2012)
  • Author(s): 麻田正仁、薄井美帆、後藤康之、横山直明、井上昇、河津信一郎
  • Organizer: 第154回日本獣医学会
  • Place of Presentation: 岩手大学
• [Presentation] Expression and immunological characterization of the heat shock protein-70 homologue from Babesia gigemina. (2012)
  • Author(s): M. Aboulaila, M. Terkawi, J. Fassoa, N. Ota, A. Caine, 横山直明、玄 学南、五十嵐郁男
  • Organizer: 第154回日本獣医学会
  • Place of Presentation: 岩手大学
• [Presentation] An improved immunochromatographic test based on the C-terminal 48-kDa rhoptry protein for rapid detection of B. caballi infection in horses (2011)
  • Author(s): Terkawi, A., Ueno, A., Goo, Y.-K., Yokoyama, N., Xuan, X., and Igarashi, I
  • Organizer: 第80回日本寄生虫学会大会
  • Place of Presentation: 東京慈恵会医科大学
• [Presentation] Validation and evaluation of recombinant antigens for serdiagnosis of Babesia bovis infection (2010)
  • Author(s): Terkawi, M.A., Seuseu, J., Wibowo, P., Aboulaila, M., Yokoyama, N., Xuan, X., and Igarashi, I
  • Organizer: 第79回日本寄生虫学会大会
  • Place of Presentation: 旭川市大雪クリスタルホール
• [Presentation] GFP発現バベシア原虫作製の試み (2010)
  • Author(s): 河津信一郎、田中みほ、後藤康之、横山直明、玄学南、井上昇
  • Organizer: 第79回日本寄生虫学会大会
  • Place of Presentation: 旭川市大雪クリスタルホール
• [Book] In Textbook of Food Safety and Animal Health, Kono, H., Nishida, T., Nishikawa, Y., Fukuda, K., Kanayama, T., and Umetsu, K. (eds.), Textbook Editorial Committee of Obihiro University of Agriculture and Veterinary Medicine, Japan (2011)
  • Author(s): Xuan, X., Inoue, N., Suzuki, H., Yokoyama, N., Fukumoto, S., Igarashi, M., and Nishikawa, Y.(分担執筆)
  • Publisher: Veterinary Protozoology
• [Remarks]
  • URL: http://www.obihiro.ac.jp/~protozoa/index.html
• [Remarks]
  • URL: http://www.obihiro.ac.jp/facility/protozoa.html