Molecular mechanism of anti-parasite response of midgut bacteria of vector insect

• Project/Area Number: 22380155
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: FUKUMOTO Shinya 帯広畜産大学, 原虫病研究センター, 准教授 (50376422)
• Co-Investigator(Kenkyū-buntansha): KANUKA Hirotaka 慈恵会医科大学, 医学部, 教授 (50342770) ; KOIKE Masanori 帯広畜産大学, 畜産学部, 教授 (00205303)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2010: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: マラリア / 腸内細菌 / ハマダラカ / セラチア

Research Abstract

To investigate the response mechanisms within the midgut environment, including those influenced by resident microbiota against malaria parasite, we focus on a midgut bacteria species intra-specific variation that confers diversity to the mosquito`s competency for malaria parasite transmission. Serratia marcescens isolated from laboratory-reared mosquitoes shows great phenotypic variation in its cellular and structural features. This variation is directly correlated with its ability to inhibit Plasmodium parasite development within the mosquito midgut. These findings indicated that strategies for controlling malaria through genetic manipulation of midgut bacteria within the mosquito.

Research Products

• [Journal Article] Multivariable analysis of host amino acids in plasma and liver during infection of malaria parasite Plasmodium yoelii (2013)
  • Author(s): Saiki, E., Nagao, K., Aonuma, H., Fukumoto, S., Xuan, X., Bannai, M.Kanuka, H
  • Journal Title: Malar J Volume: 12 Pages: 19-19
  • Peer Reviewed
• [Journal Article] Activation of Imd pathway in hemocyte confers infection resistance through humoral response in Drosophila (2013)
  • Author(s): Nelson, B., Freisinger, T., Ishii, K., Okado, K., Shinzawa, N., Fukumoto, S.Kanuka, H
  • Journal Title: Biochem Biophys Res Commun Volume: 430 Pages: 1120-5
  • Peer Reviewed
• [Journal Article] Intra-specific diversity of Serratia marcescens in Anopheles mosquito midgut defines Plasmodium transmission capacity (2013)
  • Author(s): Bando, H., Okado, K., Guelbeogo, W. M., Badolo, A., Aonuma, H., Nelson, B., Fukumoto, S., Xuan, X., Sagnon, N.Kanuka, H
  • Journal Title: Sci Rep Volume: 3 Pages: 1641-1641
  • Peer Reviewed
• [Journal Article] In vitro and in vivo antimalarial activities of T-2307, a novel arylamidine (2012)
  • Author(s): Kimura, A., Nishikawa, H., Nomura, N., Mitsuyama, J., Fukumoto, S., Inoue, N.Kawazu, S
  • Journal Title: Antimicrob Agents Chemother Volume: 56 Pages: 2191-3
  • Peer Reviewed
• [Journal Article] Development of an allele-specific, loop-mediated, isothermal amplification method (AS-LAMP) to detect the L1014F kdr-w mutation in Anopheles gambiae s. l (2012)
  • Author(s): Badolo, A., Okado, K., Guelbeogo, W. M., Aonuma, H., Bando, H., Fukumoto, S., Sagnon, N.Kanuka, H
  • Journal Title: Malar J Volume: 11 Pages: 227-227
  • Peer Reviewed
• [Journal Article] Development of an allele-specific, loop-mediated isothermal amplification method(AS-LAMP) to detect the L1014F kdr-w mutation in Anopheles gambiae s.l. (2012)
  • Author(s): Badolo A, 0kado K, Guelbeogo 〓, Aonuma H, Bando H, Fukumoto S, Sagnoll N, kanuka H.
  • Journal Title: Malaria Journal Volume: 11 Issue: 1 Pages: 227-227
  • DOI: 10.1186/1475-2875-11-227
  • Peer Reviewed
• [Journal Article] In vitro and in vivo antimalarial activities of T-2307, a novel arylamidine. (2012)
  • Author(s): Kimura, A., Nishikawa, H., Nomura, N., Mitsuyama, J., Fukumoto, S., Inoue, N.Kawazu, S.
  • Journal Title: Antimicrob Agents Chemother Volume: 56 Issue: 4 Pages: 2191-3
  • DOI: 10.1128/aac.05856-11
  • Peer Reviewed
• [Journal Article] Mitochondrial peroxidase TPx-2 is not essential in the blood and insect stages of Plasmodium berghei. (2012)
  • Author(s): Masuda-Suganuma, H., Usui, M., Fukumoto, S., Inoue, N.Kawazu, S.
  • Journal Title: Parasit Vectors Volume: 5 Issue: 1 Pages: 252-252
  • DOI: 10.1186/1756-3305-5-252
  • Peer Reviewed
• [Journal Article] The role of proboscis of the malaria vector mosquito Anopheles stephensi in host-seeking behavior (2011)
  • Author(s): Maekawa, E., Aonuma, H., Nelson, B., Yoshimura, A., Tokunaga, F., Fukumoto, S.Kanuka, H
  • Journal Title: Parasit Vectors Volume: 4 Pages: 10-10
  • Peer Reviewed
• [Journal Article] Improvement of the observational method for Plasmodium berghei oocysts in the midgut of mosquitoes (2011)
  • Author(s): Usui, M., Fukumoto, S., Inoue, N.Kawazu, S
  • Journal Title: Parasit Vectors Volume: 4 Pages: 118-118
  • Peer Reviewed
• [Journal Article] Calcium signal regulates temperature-dependent transformation of sporozoites in malaria parasite development (2011)
  • Author(s): Doi, Y., Shinzawa, N., Fukumoto, S., Okano, H. Kanuka, H
  • Journal Title: Exp Parasitol Volume: 128 Pages: 176-80
  • Peer Reviewed
• [Journal Article] Improvement of the observational method for Plasmodium berghei oocysts in the midgut of mosquitoes (2011)
  • Author(s): Usui, M. Fukumoto, S. Inoue, N. Kawazu, S.
  • Journal Title: Parasit Vectors Volume: 4 Issue: 1 Pages: 118-118
  • DOI: 10.1186/1756-3305-4-118
  • Peer Reviewed
• [Journal Article] The role of proboscis of the malaria vector mosquito Anopheles Stephensi in host-seeking behavior (2011)
  • Author(s): Maekawa E, Aonuma H, Nelson B, Yoshimura A, Tokunaga F, Fukumoto S, Kanuka H
  • Journal Title: Parasit Vectors Volume: 4 Pages: 10-10
  • Peer Reviewed
• [Journal Article] A single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes (2010)
  • Author(s): Aonuma, H., Yoshimura, A., Kobayashi, T., Okado, K., Badolo, A., Nelson, B., Kanuka, H. Fukumoto, S
  • Journal Title: Exp Parasitol Volume: 125 Pages: 179-83
  • Peer Reviewed
• [Journal Article] ADF2 is required for transformation of the ookinete and sporozoite in malaria parasite development (2010)
  • Author(s): Doi, Y., Shinzawa, N., Fukumoto, S., Okano, H. Kanuka, H
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Pages: 668-72
  • Peer Reviewed
• [Journal Article] A single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes (2010)
  • Author(s): Aonuma H, Yoshimura A, Kobayashi T, Okado K, Badolo A, Nelson B, Kanuka H, Fukumoto S
  • Journal Title: Exp Parasitol Volume: 125 Pages: 179-183
  • Peer Reviewed
• [Journal Article] ADF2 is required for transformation of the ookinete and sporozoite in malaria parasite development (2010)
  • Author(s): Doi Y, Shinzawa N, Fukumoto S, Okano H, Kanuka H
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Pages: 668-672
  • Peer Reviewed
• [Presentation] 野生蚊の虫体および虫体内から分離された昆虫寄生性アナモルフ菌類のAnopheles stephensi雌成虫に対する致死性・感染性の比較 (2014)
  • Author(s): 石井嶺広、竹下準也,石山海嗣,小池正徳,嘉糠洋陸,福本晋也,相内大吾
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学(奈良県奈良市)
• [Presentation] 西アフリカに生息するマラリア媒介蚊の腸内細菌種内多様性に関する研究 (2013)
  • Author(s): 伴戸寛徳、岡戸清、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 第156回日本獣医学会
  • Place of Presentation: 岐阜大学(岐阜県岐阜市)
• [Presentation] 非共生細菌の表現型揺らぎが規定するベクター・寄生虫間相互作用 (2013)
  • Author(s): 嘉糠洋陸、伴戸寛徳、岡戸清、Guelbego Wamdaogo、Badolo Athanase、青沼宏佳、福本晋也、NFale Sagnon
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学(東京都文京区)
• [Presentation] Amino acid‒related host nutrition dynamics during malaria infection. (2013)
  • Author(s): Saiki E, Nagao K, Fukumoto S, Bannai M, Kanuka H
  • Organizer: Keystone Symposia on Molecular and Cellular Biology : Malaria.
  • Place of Presentation: JW Marriott New Orleans, New Orleans, Louisiana USA
• [Presentation] 非共生細菌の表現型揺らぎが規定するベクター・寄生虫間相互作用 (2013)
  • Author(s): 嘉糠洋陸、伴戸寛徳、岡戸清、Guelbego Wamdaogo､Badolo Athanase､青沼宏佳、福本晋也、NFale Sagnon
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学（ 東京都文京区 ）
• [Presentation] マラリア原虫感染時の宿主血中アミノ酸インフォマティクス (2013)
  • Author(s): 齋木選射、長尾健児、福本晋也、坂内慎、嘉糠洋陸
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学（ 東京都文京区 ）
• [Presentation] Amino acid‒related host nutrition dynamics during malaria infection. (2012)
  • Author(s): Saiki E, Nagao K, Fukumoto S, Bannai M, Kanuka H.
  • Organizer: 第14回日韓寄生虫学セミナー
  • Place of Presentation: 青島パームビーチホテル（ 宮崎県宮崎市 ）
• [Presentation] 非共生細菌の表現型揺らぎが規定する宿主-寄生虫間相互作用 (2011)
  • Author(s): 伴戸寛徳、岡戸清、Moussa Guelbeogo、Athanase Badolo、青沼宏佳、福本晋也、N'Fale Sagnon、嘉糠洋陸
  • Organizer: 第71回日本寄生虫学会東日本支部大会
  • Place of Presentation: 国立科学博物館(東京都台東区)
  • Year and Date: 2011-10-01
• [Presentation] 非共生細菌の表現型揺らぎが規定する宿主-寄生虫間相互作用 (2011)
  • Author(s): 伴戸寛徳、岡戸清、Moussa Guelbeogo、Athanase Badolo、青沼宏佳、福本晋也、N'Fale Sagnon、嘉糠洋陸
  • Organizer: 第71回日本寄生虫学会東日本支部大会
  • Place of Presentation: 杏林大学(東京都三鷹市)
  • Year and Date: 2011-10-01
• [Presentation] 野生蚊由来の昆虫寄生性アナモルフ菌類の分離およびその潜在感染率の評価 (2011)
  • Author(s): 竹下準也,石山海嗣,小池正徳,福本晋也,嘉糠洋陸,相内大吾
  • Organizer: 第55回日本応用動物昆虫学会大会
  • Place of Presentation: 九州大学(福岡県福岡市)
  • Year and Date: 2011-03-27
• [Presentation] Anopheles stephensi雌成虫に対する蚊由来昆虫寄生性アナモルフ菌類の感染性の評価 (2011)
  • Author(s): 石山海嗣,竹下隼也,小池正徳,福本晋也,嘉糠洋陸,相内大吾
  • Organizer: 第55回日本応用動物昆虫学会大会
  • Place of Presentation: 九州大学(福岡県福岡市)
  • Year and Date: 2011-03-27
• [Presentation] 非共生細菌の表現型揺らぎが規定する宿主-寄生虫間相互作用 (2011)
  • Author(s): 伴戸寛徳、青沼宏佳、N'Fale Sagnon、福本晋也、嘉糠洋陸
  • Organizer: 第9回感染症沖縄フォーラム
  • Place of Presentation: 沖縄国民年金健康センター、(沖縄県宜野湾市)
  • Year and Date: 2011-02-11
• [Presentation] 腸内細菌の"ゆらぎ"が宿主-病原体相互作用に与える影響 (2011)
  • Author(s): 伴戸寛徳、岡戸清、Moussa Guelbeogo、Athanase Badolo、青沼宏佳、福本晋也、N'Fale Sagnon、嘉糠洋陸
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
• [Presentation] 腸内細菌の“ゆらぎ”が宿主-病原体相互作用に与える影響 (2011)
  • Author(s): 伴戸寛徳、岡戸清、Moussa Guelbeogo、Athanase Badolo、青沼宏佳、福本晋也、N’Fale Sagnon、嘉糠洋陸
  • Organizer: 第34 回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Midgut-based insect and parasite interaction in malaria vector Anopheles mosquitoes (2011)
  • Author(s): Hironori Bando, Hiroka Aonuma, Kiyoshi Okado, Naoaki Shinzawa, Guelbeogo Moussa, N’Fale Sagnon, Shinya Fukumoto, and Hirotaka Kanuka
  • Organizer: 第５回病原体媒介節足動物国際会議
  • Place of Presentation: ギリシャ
• [Presentation] 宿主-病原体相互作用における腸管内細菌の"ゆらぎ" (2010)
  • Author(s): 伴戸寛徳、青沼宏佳、N'Fale Sagnon、福本晋也、嘉糠洋陸
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場(兵庫県神戸市)
  • Year and Date: 2010-12-08
• [Presentation] 宿主-病原体相互作用における腸管内細菌のゆらぎ (2010)
  • Author(s): 宿主-病原体相互作用
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Midgut bacteria regulates Plasmodium development in malaria vector Anopheles mosquitoes (2010)
  • Author(s): 伴戸寛徳、青沼宏佳、岡戸清、新澤直明、Moussa Guelbeogo、N'Fale Sagnon、福本晋也、嘉糠洋陸
  • Organizer: Keystone Symposia "Molecular Targets for Control of Vector-Borne Diseases : Bridging Lab and Field Research
  • Place of Presentation: Copper Mountain Resort(Copper mountain, United States)
  • Year and Date: 2010-04-14