Acquisition of the meiotic and mitotic competence during oocyte growth

• Project/Area Number: 22380156
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: The University of Tokyo
• Principal Investigator: AOKI Fugaku 東京大学, 大学院・新領域創成科学研究科, 教授 (20175160)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: 発生 / 成熟能 / 発生能 / 卵 / 初期胚 / 卵成熟 / 初期発生 / 母性因子 / 卵成長

Research Abstract

Mammalian oocytes acquire the competence for meiotic maturation ad subsequent development during their growth in the ovaries. However, a part of fully grown oocytes do not have this competence. In the present study, to determine the factor(s) involved in this competence, I conducted the transcriptome analysis for the oocytes with and without the competence. Several candidates for the factor were obtained from this analysis. By inhibiting the expression of these candidates in the growing oocytes, I determined the target factors.

Research Products

• [Journal Article] The expression and nuclear deposition of histone H3.1 in murine oocytes and preimplantation embryos (2012)
  • Author(s): Kawamura M, Akiyama T, Tsukamoto S, Suzuki MG & Aoki F
  • Journal Title: J. Reprod. Dev Volume: 28 Pages: 557-562
  • NAID: 10031123300
  • URL: https://www.jstage.jst.go.jp/article/jrd/58/5/58_2012-074/_article
  • Peer Reviewed
• [Journal Article] The expression and nuclear deposition of histone H3.1 in murine oocytes and preimplantation embryos (2012)
  • Author(s): Kawamura M
  • Journal Title: J. Reprod. Dev Volume: 58 Pages: 557-562
  • Peer Reviewed
• [Journal Article] Effect of RNAi-mediated knockdown of the Bombyx mori transformer-2 gene on the sex specific splicing of Bmdsx pre-mRNA. (2012)
  • Author(s): Suzuki MG, Aoki F, 他2名
  • Journal Title: Int. J. Dev. Biol. Volume: 56 Issue: 9 Pages: 693-699
  • DOI: 10.1387/ijdb.120049ms
  • Peer Reviewed
• [Journal Article] Dramatic replacement of histone variants during genome remodeling in nuclear-transferred embryos (2011)
  • Author(s): Nashun B, Akiyama T, Suzuki MG & Aoki F
  • Journal Title: Epigenetics Volume: 6 Issue: 12 Pages: 1489-1497
  • DOI: 10.4161/epi.6.12.18206
  • Peer Reviewed
• [Journal Article] Involvement of murine nucleoplasmin 2 in the decondensation of sperm chromatin after fertilization (2011)
  • Author(s): Inoue A, Ogushi S, Saitou M, Suzuki MG& Aoki F
  • Journal Title: Biol. Reprod Volume: 85 Pages: 70-77
  • Peer Reviewed
• [Journal Article] Global gene silencing is caused by the dissociation of RNA polymerase II from DNA in mouse oocytes (2010)
  • Author(s): Abe K, Inoue A, Suzuki MG & Aoki F
  • Journal Title: J. Reprod. Dev Volume: 56 Pages: 502-507
  • NAID: 10027571758
  • URL: https://www.jstage.jst.go.jp/article/jrd/56/5/56_10-068A/_article
  • Peer Reviewed
• [Journal Article] Global gene silencing is caused by the dissociation of RNA polvmerase II from DNA in mouse oocytes. (2010)
  • Author(s): Abe, K
  • Journal Title: J.Reprod.Dev. Volume: 56 Pages: 502-507
  • Peer Reviewed
• [Presentation] マウス着床前初期胚におけるヒストンH2Bユビキチン化 (2013)
  • Author(s): 大我政敏、青木不学
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 広島
  • Year and Date: 2013-03-30
• [Presentation] マウス受精前後における母性mRNA分解に関する網羅的な動態解析 (2013)
  • Author(s): 金綾奈、鈴木元、阿部健一郎、青木不学
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 広島
  • Year and Date: 2013-03-28
• [Presentation] RNAシーケンスによるマウス一細胞期胚の遺伝子発現解析 (2013)
  • Author(s): 阿部健一郎、山本龍馬、曹旻君、鈴木穣、青木不学
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 広島
  • Year and Date: 2013-03-28
• [Presentation] Characterization of the geneexpression in the 1-cell stage embryos (2012)
  • Author(s): Abe K, Yamamoto R, Oka G, Franke V, Vlahovicek K, Suzuki Y & Aoki F
  • Organizer: The American Society for Cell Biology
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-12-18
• [Presentation] Histone H2B ubiquitination in the mouse preimplantation embryos (2012)
  • Author(s): Ooga M & Aoki F
  • Organizer: The American Society for Cell Biology
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-12-18
• [Presentation] The profile of the genes transcribed at the onset of gene expression after fertilization (2012)
  • Author(s): Yamamoto R, Abe K, Franke V, Vlahovicek K, Suzuki Y & Aoki F
  • Organizer: The American Society for Cell Biology
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-12-18
• [Presentation] 受精前後におけるゲノムリプログラミングへのヒストン変異体置換の関与 (2012)
  • Author(s): 青木不学
  • Organizer: 日本畜産学会
  • Place of Presentation: 名古屋大学(名古屋市)(招待講演)
  • Year and Date: 2012-03-29
• [Presentation] Genome-scale profiling of the chromatin composition of histone H2A and H3 variants in mouse embryonic stem cells (2012)
  • Author(s): Aoki F
  • Organizer: The 24th Annual Meeting of the Korean Society for Molecular and Cellular Biology
  • Place of Presentation: Seoul, Korea
• [Presentation] マウス着床前初期胚におけるHistoneH3lysine79メチル化の調節機構について (2012)
  • Author(s): 大我政敏、青木不学
  • Organizer: 第115回日本畜産学会
  • Place of Presentation: 名古屋
• [Presentation] 発生過程におけるゲノムリモデリングへのH3変異体の関与について (2012)
  • Author(s): 河村真愛、秋山智彦、塚本智史、青木不学
  • Organizer: 第115回日本畜産学会
  • Place of Presentation: 名古屋
• [Presentation] Mechanism of global gene silencing and reactivation during oocvte growth at the one-cell stage after fertilization in mice (2010)
  • Author(s): Abe K
  • Organizer: 69^<th>Annual Meeting of Society for Devel opmental Biologv
  • Place of Presentation: Albuquerque, USA
  • Year and Date: 2010-08-07
• [Book] 卵子学
  • Author(s): 青木不学
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/seigyo/index.html