Generation of transgenic mice expressing soluble forms of Siglecs as a model for influenza-resistant pigs

• Project/Area Number: 22380161
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Kyushu University
• Principal Investigator: ONO Etsuro 九州大学, 医学研究院, 教授 (00160903)
• Co-Investigator(Renkei-kenkyūsha): TOMIOKA Yukiko 北海道大学, 遺伝子病制御研究所, 助教 (50374674)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2010: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
• Keywords: インフルエンザ / トランスジェニックマウス / ウイルス / 抗病性動物 / Siglec / 感染症 / トランスジェニクマウス

Research Abstract

To confer the antiviral potential against influenza virus to pigs as a mixing vessel for creating a “new” influenza virus, we investigated the ability of Siglecs to inhibit influenza virus infection in vitro and in vivo by using Madin-Derby canine kidney (MDCK) cells and transgenic mice expressing a soluble form of human Siglec molecules. The transformed MDCK cells showed a significant resistance, but not the transgenic mice

Research Products

• [Presentation] Antiviral effects of anti-PB2 monoclonal intrabody inhibiting the viral RNA transcription on influenza virus infections (2012)
  • Author(s): Yoshikazu Fujimoto, Kinuyo Ozaki, Hiroki Takakuwa, Hiroshi Ito, Tatsufumi Usui, Hiroichi Ozaki, Toshiyuki Murase, Tsuyoshi Yamaguchi, Koichi Otsuki, Toshihiro Ito, Hiroshi Kida and Etsuro Ono
  • Organizer: 9th International Congress of Veterinary Virology
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2012-09-04
• [Presentation] Possible antiviral potential of soluble forms of Siglecs in influenza virus infection (2011)
  • Author(s): Tatsufumi Usui, Kinuyo Ozaki, Hiroki Takakuwa, Hiroichi Ozaki, Hiroshi Ito, Toshiyuki Murase, Koichi Otsuki, Toshihiro Ito, Tsuyoshi Yamaguchi and Etsuro Ono
  • Organizer: 5th Annual Meeting EPIZONE
  • Place of Presentation: Arnhem, The Netherlands
  • Year and Date: 2011-04-12
• [Presentation] Possible antiviral potential of soluble forms of Siglecs in influenza virusinfection (2011)
  • Author(s): Tatsufumi Usui, Kinuyo Ozaki, Hiroki Takakuwa, Hiroichi Ozaki, Hiroshi Ito, Toshiyuki Murase, Koichi Otsuki, Toshihiro Ito, Tsuyoshi Yamaguchi, Etsuro Ono
  • Organizer: 5th Annual Meeting EPIZONE
  • Place of Presentation: Arnhem, The Netherlands
  • Year and Date: 2011-04-12