Identification and characterization of MHC-assigned swine hematopoietic stem cells: availability for the human immunity model animals
| Project/Area Number |
22380172
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHBA Yasunori 岐阜大学, 応用生物科学部, 准教授 (20377691)
KAMETANI Yoshie 東海大学, 医学部, 講師 (50338787)
ANDO Asako 東海大学, 医学部, 准教授 (40101935)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | MHC 固定ブタ / 造血幹細胞 / 抗ブタ c-kit 抗体 / 再生医療 / 造血幹細胞マーカー / ブタ / MHC / MHC固定ブタ / リンパ球分化マーカー / 免疫不全NOGマウス |
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| Research Abstract |
c-kit molecule is a marker of the wide variety of tissue stem cells. Thus, the utility has been prominently upregulated. However, there are no good monoclonal antibodies against swine c-kit molecule. We prepared the anti-swine c-kit monoclonal antibody with high specificity, mainly available for flow-cytometrical analysis. New-born bone marrow cells contained higher ratio of c-kit positive cells compared to adult bone marrow cells or cord blood cells. The purified swine bone marrow c-kit+ cells mainly developed red blood cells by conventional colony assay with human cytokines. Moreover, transplantation of the c-kit+ cells developed swine CD45+ cells, suggesting that the white blood cells were successfully developed from swine hematopoeticstem/progenitor cells in the immune-deficient NOG mice. The anti-swine c-kit monoclonal antibody did not recognize mouse or human mast cells, suggesting the antibody is species-specific. These results suggest that the c-kit monoclonal antibody prepared by us will become a powerful tool for the study of swine stem cell biology.
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Report
(4 results)
Research Products
(91 results)
