Applied research of phage therapy for bovine mastitis

• Project/Area Number: 22380174
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Rakuno Gakuen University
• Principal Investigator: IWANO Hidetomo 酪農学園大学, 獣医学群, 准教授 (60382488)
• Co-Investigator(Kenkyū-buntansha): HIGUCHI Hidetosi 酪農学園大学, 獣医学群, 准教授 (00305905) ; INOUE Hiroki 酪農学園大学, 食品環境学群, 准教授 (90305904)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: ファージセラピー / 黄色ブドウ球菌 / 牛乳房炎 / MRSA / ウシ乳房炎 / 感染症 / 獣医学 / 微生物 / 抗生物質 / 医療・福祉

Research Abstract

In this work, we isolated novel two bacteriophages for Staphylococcus aureus （SA） with wide host ranges from sewage, and examined carefully their potential as therapeutic agents using experimental murine mastitis. All 99 strains of SA isolated from distinct ruminants with mastitis were completely lysed by SA bacteriophage. SA bacteriophage inhibited the SA growth in the mammary gland of experimental murine mastitis.

Research Products

• [Journal Article] Effects of vitamin E supplementation on cellular a-tocopherol concentrations of neutrophils in Holstein calves (2012)
  • Author(s): Higuchi, H., Ito, E., Iwano, H., Oikawa, S. and Nagahata, H
  • Journal Title: Can. J. Vet. Res
• [Journal Article] Analysis of trace and major elements in bronchoalveolar lavage fluid of Mycoplasma bronchopneumonia in calves (2012)
  • Author(s): Suzuki K, Higuchi H, Iwano H, Lakritz J, Sera K, Koiwa M, Taguchi K
  • Journal Title: Biol. Trace. Elem. Res.Feb Volume: 145(2):166-71
• [Journal Article] L-Type Amino Acid Transporter 1 (LAT1) Expression in Canine Mammary Gland Tumors (2012)
  • Author(s): Fukumoto S, Hanazono K, Komatsu T, Iwano H, Kadosawa T, Uchide T
  • Journal Title: J. Vet. Med. Sci. Nov Volume: 22
• [Journal Article] Pathological classification of canine mammary tumor based on quantifying mRNA levels of hormonal receptors, SATB1, and snail in tissue and fine needle biopsy samples (2012)
  • Author(s): Komatsu T, Iwano H, Ebisawa M, Watabe A, Endo Y, Hirayama K, Taniyama H, Kadosawa T
  • Journal Title: J.Vet. Med. Sci Volume: 74(6):719-26
• [Journal Article] Analysis of trace and major elements in bronchoalveolar lavage fluid of Mycoplasma bronchopneumonia in calves. (2012)
  • Author(s): Suzuki K, Higuchi H, Iwano H, Lakritz J, Sera K, Koiwa M, Taguchi K.
  • Journal Title: Biol. Trace. Elem. Res. Volume: 145 Pages: 166-171
  • Peer Reviewed
• [Journal Article] Effectiveness of slightly acidic electrolyzed water for improvement of hygienic conditions of teat liners of automatic milking system. (2012)
  • Author(s): Nagahata, H., Yuge, K., Moriyama, A., Takahashi, A., Iwano, H., Higuchi, H., Mitamura, T. and Matsuyama, H.
  • Journal Title: Milchwissenschaft, Volume: 67 Pages: 123-126
  • Peer Reviewed
• [Journal Article] Pathological classification of canine mammary tumor based on quantifying mRNA levels of hormonal receptors, SATB1, and snail in tissue and fine needle biopsy samples. (2012)
  • Author(s): Komatsu T, Iwano H, Ebisawa M, Watabe A, Endo Y, Hirayama K, Taniyama H, Kadosawa T.
  • Journal Title: J. Vet. Med. Sci. Volume: 74 Pages: 719-726
  • Peer Reviewed
• [Journal Article] A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle (2011)
  • Author(s): Higuchi, H., Iwano, H., Kawai, K., Ohta, T., Obayashi, T., Hirose, K., Itoh, N., Yokota, T., Tamura, Y. and Nagahata, H
  • Journal Title: J. Vet. Sci Volume: 12: 191-193
  • NAID: 120005761687
• [Journal Article] Prevalence of Mycoplasma species in bulk tank milk in Japan (2011)
  • Author(s): Higuchi, H., Iwano, H., Gondaira, S., Kawai, K., Nagahata, H
  • Journal Title: Vet. Rec Volume: 169:442
• [Journal Article] Analysis of trace and major elements in bronchoalveolar lavage fluid of mycoplasma bronchopneumonia in calves (2011)
  • Author(s): Suzuki, K., Higuchi, H., Iwano, H., Lakritz, J., Sera, K., Koiwa, M., Taguchi, K
  • Journal Title: Biol. Trace. Elem. Res Volume: 145:166-171
• [Journal Article] A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle (2011)
  • Author(s): Higuchi, H., Iwano, H., Kawai, K., Ohta, T., Obayashi, T., Hirose, K., Itoh, N., Yokota, T., Tamura, Y., Nagahata, H.
  • Journal Title: J.Vet.Sci. Volume: 12 Pages: 191-193
  • NAID: 120005761687
  • Peer Reviewed
• [Journal Article] Prevalence of Mycoplasma species in bulk tank milk in Japan (2011)
  • Author(s): Higuchi, H., Iwano, H., Gondaira, S., Kawai, K., Nagahata, H.
  • Journal Title: Vet.Rec. Volume: 169 Pages: 442-443
  • Peer Reviewed
• [Journal Article] Analysis of trace and major elements in bronchoalveolar lavage fluid of mycoplasma bronchopneumonia in calves (2011)
  • Author(s): Suzuki, K., Higuchi, H., Iwano, H., Lakritz, J., Sera, K., Koiwa, M., Taguchi, K.
  • Journal Title: Biol.Trace.Elem.Res. Volume: 145 Pages: 166-171
  • Peer Reviewed
• [Journal Article] 黄色ブドウ球菌に対する特異的なファージを用いたウシ乳房炎治療応用への可能性 (2011)
  • Author(s): 岩野英知、樋口豪紀、井上博紀、萩原克郎、横田博、永幡肇、丹治保典、田村豊
  • Journal Title: 日本乳房炎研究会Proceedings Volume: 第15回 Pages: 41-44
  • Peer Reviewed
• [Journal Article] Placental Transfer of Conjugated Bisphenol A and Subsequent Reactivation in the Rat fetus (2010)
  • Author(s): Nishikawa M., Iwano H., Yanagisawa R., Koike N., Inoue H., and Yokota H
  • Journal Title: Environ. Health Perspect Volume: 118 Pages: 1196-1203
  • NAID: 120005761579
• [Journal Article] Histone H1 null vertebrate cells exhibit altered nucleosome architecture (2010)
  • Author(s): Hashimoto H, Takami Y, Sonoda E, Iwasaki T, Iwano H, Tachibana M, Takeda S, Nakayama T, Kimura H, Shinkai Y
  • Journal Title: Nucleic Acids Res Volume: 383533-45
• [Presentation] Characterization of novel Staphylococcus aureus bacteriophages with wide host ranges and their potential as therapeutic agents for bovine mastitis. (2012)
  • Author(s): Iwano, H., Higuchi, H., Inoue, H., Kobayashi, H., Nagahata, H., Yokota, H., Tanji, Y., Tamura, Y
  • Organizer: World buiatrics congress 2012
  • Place of Presentation: Lisbon
• [Presentation] 乳房炎由来ブドウ球菌に対するバクテリオファージの有効性と体内動態 (2012)
  • Author(s): 小林弘典、 岩野英知、 樋口豪紀、 井上博紀、萩原克郎、丹治保典、永幡肇、田村豊、横田博
  • Organizer: 第153回日本獣医学会学術集会
• [Presentation] Mycoplasma bovisがウシ単核球サイトカイン mRNA の発現量に及ぼす影響 (2012)
  • Author(s): 権平智、樋口豪紀、岩野英知、永幡肇
  • Organizer: 第153回日本獣医学会学術集会
• [Presentation] Mycoplasma bovisがウシ単核球サイトカインmRNAの発現量に及ぼす影響 (2012)
  • Author(s): 権平 智、樋口豪紀、岩野英知、永幡 肇
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 江別
• [Presentation] 乳房炎由来ブドウ球菌に対するバクテリオファージの有効性と体内動態 (2012)
  • Author(s): 小林弘典、岩野英知、樋口豪紀、井上博紀、井上裕介、萩原克郎、丹治保典、永幡肇、田村豊、横田博
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 大宮ソニックシティー
• [Presentation] マイコプラズマ性乳房炎の摘発を目的としたバルクスクリーニングの実施とその評価 (2011)
  • Author(s): 樋口豪紀、岩野英知、河合一洋、永幡肇
  • Organizer: 平成 23 年度北海道獣医師大会
• [Presentation] ウシ乳房炎由来ブドウ球菌に対するバクテリオファージの有効性と体内動態 (2011)
  • Author(s): 小林弘典、岩野英知、樋口豪紀、井上博紀、丹治保典、永幡肇、横田博、田村豊
  • Organizer: 第152回日本獣医学会学術集会
• [Presentation] ウシ乳房炎由来ブドウ球菌に対するバクテリオファージの有効性と体内動態 (2011)
  • Author(s): 小林弘典、岩野英知、樋口豪紀、井上博紀、井上裕介、萩原克郎、丹治保典、永幡肇、田村豊、横田博
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
• [Presentation] 黄色ブドウ球菌の乳腺感染モデルマウスに対するファージセラピーの検討 (2010)
  • Author(s): 岩野英知、樋口豪紀、井上博紀、井上裕介、高砂拓志、萩原克郎、丹治保典、永幡肇、横田博
  • Organizer: 第150回日本獣医学会学術集会
• [Presentation] 北海道におけるウシ乳房炎由来マイコプラズマ種の浸潤調査 (2010)
  • Author(s): 伊藤暢彦、樋口豪紀、岩野英知、河合一洋、大林哲、永幡肇
  • Organizer: 平成 22 年度北海道獣医師大会
• [Presentation] 黄色ブドウ球菌に対する特異的なファージを用いたウシ乳房炎治療応用への可能性 (2010)
  • Author(s): 岩野英知、樋口豪紀、 井上博紀、萩原克郎、横田博、永幡肇、丹治保典、田村豊
  • Organizer: 乳房炎研究会
• [Presentation] 黄色ブドウ球菌感染症へのファージ療法の応用 (2010)
  • Author(s): 岩野英知、樋口豪紀、井上博紀、横田博、永幡肇、小倉俊樹、近藤昭宏、丹治保典、田村豊
  • Organizer: 動物用ワクチンーバイオ医薬品研究会設立記念シンポジウム
  • Place of Presentation: 日本獣医生命科学大学
• [Presentation] ウシ乳房炎由来黄色ブドウ球菌に対するバクテリオファージの有効性 (2010)
  • Author(s): 井上裕介、英知、樋口豪紀、井上博紀、高砂拓志、萩原克郎、丹治保典、永幡肇、田村豊、横田博
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学・帯広
• [Presentation] 黄色ブドウ球菌に対する特異的なファージを用いたウシ乳房炎治療応用への可能性 (2010)
  • Author(s): 岩野英知、樋口豪紀、井上博紀、萩原克郎、横田博、永幡肇、丹治保典、田村豊
  • Organizer: 日本乳房炎研究会第15回学術集会
  • Place of Presentation: つくば国際会議場・茨城県つくば市
• [Presentation] 北海道における子ウシ鼻腔内Mycoplasma 種の浸潤調査
  • Author(s): 樋口豪紀、岩野英知、河合一洋、 榊原優子、佐藤豊孝、 小岩政照、 田村豊、 永幡肇
  • Organizer: 平成24年度獣医学術学会北海道地区学会・北海道獣医師大会
  • Place of Presentation: 江別
• [Presentation] Characterization of novel Staphylococcus aureus bacteriophages with wide host ranges and their potential as therapeutic agents for bovine mastitis.
  • Author(s): Iwano, H., Higuchi, H., Inoue, H., Kobayashi, H., Nagahata, H., Yokota, H., Tanji, Y., Tamura, Y.
  • Organizer: World buiatrics congress 2012
  • Place of Presentation: リスボン(ポルトガル)
• [Book] 「バクテリオファージ」による乳房炎治療への応用酪農ジャーナル (2012)
  • Author(s): 岩野英知
  • Publisher: 酪農学園大学エクステンションセンター
• [Book] 細菌の生き残り戦略～最も繁栄している生物の仕組み～ (2012)
  • Author(s): 岩野英知
  • Publisher: The Japanese Society for Animal Vaccine and Biomedical Research NEWS letter
• [Book] 犬と猫の早期疾病診断学 (2011)
  • Author(s): 岩野英知, 予防動物医学研究会編(新井敏郎ほか)
  • Publisher: チクサン出版社(緑書房)