Response of soil microbial community to exposure of environmental pollutants

• Project/Area Number: 22380176
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Tohoku University
• Principal Investigator: TSUDA Masataka 東北大学, 大学院・生命科学研究科, 教授 (90172022)
• Co-Investigator(Kenkyū-buntansha): KUROKAWA Ken 東京工業大学, 大学院・生命理工学研究科, 教授 (20343246) ; NAGATA Yuji 東北大学, 大学院・生命科学研究科, 准教授 (30237531) ; OHTSUBO Yoshiyuki 東北大学, 大学院・生命科学研究科, 助教 (40342761)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 環境汚染 / 細菌 / メタゲノム / 分解酵素遺伝子

Research Abstract

Soil microbial communities play very important role in the bioremediation of chemical pollutants. To investigate how such a community responded to and degrade the pollutants, time series analysis of the community in an artificially polluted and closed soil system was carried out by metagenomic sequencing of the community in conjunction with the isolation and characterization of the genes and bacterial strains able to degrade the pollutants. Our analysis strongly suggested the crucial catabolic genes and their host genera involved in the bioremediation in the polluted soil.

Research Products

• [Journal Article] Complete genome sequence of a Propionibacterium acnes isolate from a sarcoidosis patient. (2013)
  • Author(s): Minegishi K, Aikawa C, Furukawa A, Watanabe T, Nakano T, Ogura Y, Ohtsubo Y, Kurokawa K, Hayashi T, Maruyama F, Nakagawa I, Eishi Y
  • Journal Title: Genome Announcement Volume: 1 Issue: 1 Pages: 16-12
  • DOI: 10.1128/genomea.00016-12
  • Peer Reviewed
• [Journal Article] Coinetgrate-resolution of toluene-catabolic transposon Tn4651 (2013)
  • Author(s): Yano, H., H. Genka, Y. Ohtsubo, Y. Nagata, E.M. Top, M. Tsuda
  • Journal Title: Plasmid Volume: 69 Issue: 1 Pages: 24-35
  • DOI: 10.1016/j.plasmid.2012.07.004
  • Peer Reviewed
• [Journal Article] Complete genome sequence of the γ-hexachlorocyclohexane-degrading bacterium Sphingomonas sp. strain MM-1 (2013)
  • Author(s): Michiro Tabata
  • Journal Title: Genome Announcements Volume: 1
  • Peer Reviewed
• [Journal Article] 機能相補による芳香族化合物複合汚染土壌からの新規分解酵素遺伝子の探索 (2013)
  • Author(s): 永山浩史
  • Journal Title: Journal of Environmental Biotechnology Volume: 13
  • Peer Reviewed
• [Journal Article] GHOSTM: A GPU-Accelerated Homology Search Tool for Metagenomics (2012)
  • Author(s): Suzuki S, Ishida T, Kurokawa K, Akiyama Y
  • Journal Title: PLoS ONE Volume: 7 Issue: 5 Pages: 1-8
  • DOI: 10.1371/journal.pone.0036060
  • Peer Reviewed
• [Journal Article] Conjugal transfer of polychlorinated biphenyl/biphenyl degradation genes in Acidovorax sp. KKS102 located on integrative and conjugative element (2012)
  • Author(s): Y. Ohtsubo, 4人, Y. Nagata, and M. Tsuda
  • Journal Title: J. Bacteriol Volume: 184 Pages: 4237-4248
  • Peer Reviewed
• [Journal Article] Suppression of pleiotrophic phenotypes of Burkholderia multivorans fur mutant by oxyR mutation (2012)
  • Author(s): Kimura, S. Yuhara, Y. Ohtsubo, Y. Nagata, and M. Tsuda
  • Journal Title: Microbiology Volume: 158 Pages: 1284-1293
  • Peer Reviewed
• [Journal Article] Suppression of pleiotrophic phenotypes of Burkholderia multivorans fur mutant by oxyR mutation (2012)
  • Author(s): Kimura, A., S. Yuhara, Y. Ohtsubo, Y. Nagata, and M. Tsuda
  • Journal Title: Microbiology Volume: 158 Issue: 5 Pages: 1284-1293
  • DOI: 10.1099/mic.0.057372-0
  • Peer Reviewed
• [Journal Article] Povotal role of anthranilate dioxygenase genes in the adaptation of Burkholderia multivorans ATCC17616 in soil (2012)
  • Author(s): Nishiyama, E., Y. Ohtsubo, Y. Yamamoto, Y. Nagata, and M. Tsuda
  • Journal Title: FEMS Microbiology Letters Volume: 330 Issue: 1 Pages: 46-55
  • DOI: 10.1111/j.1574-6968.2012.02532.x
  • Peer Reviewed
• [Journal Article] Cloning ofγ-hexachlorocyclohexane dehydrochlorinase gene with its flanking regions from soil byactivity-based screening techniques (2012)
  • Author(s): Ito, M., A. Ono, Y. Ohtsubo, M. Tsuda, and Y. Nagata
  • Journal Title: European J. Soil Biology Volume: 52 Pages: 16-19
  • DOI: 10.1016/j.ejsobi.2012.05.001
  • Peer Reviewed
• [Journal Article] Conjugal transfer of polychlorinated biphenyl/biphenyl degradation genes in Acidovorax sp. strain KKS102, which are located on an integrative and conjugative element (2012)
  • Author(s): Ohtsubo Y., Y. Ishibashi, H. Naganawa, S. Hirokawa, S. Atobe, Y. Nagata, M. Tsuda
  • Journal Title: J Bacteriol Volume: 194 Issue: 16 Pages: 4237-4248
  • DOI: 10.1128/jb.00352-12
  • Peer Reviewed
• [Journal Article] ParI, an orphan ParA family protein from Pseudomonas putida KT2440-specific genomic island, interferes with the partition system of IncP-7 plasmids (2012)
  • Author(s): Miyakoshi, M., M. Shintani, K. Inoue, T. Terabayashi, F. Sai, M. Ohkuma, H. Nojiri, Y. Nagata, M. Tsuda
  • Journal Title: Environ Microbiol Volume: 14 Issue: 11 Pages: 2946-2959
  • DOI: 10.1111/j.1462-2920.2012.02861.x
  • Peer Reviewed
• [Journal Article] Complete genome sequence of Acidovorax sp. KKS102, a polychlorinated biphenyl-degrading strain (2012)
  • Author(s): Ohtsubo, Y., F. Maruyama, H. Mitsui, Y. Nagata, M. Tsuda
  • Journal Title: J Bacteriol Volume: 194 Issue: 24 Pages: 6970-6971
  • DOI: 10.1128/jb.01848-12
  • Peer Reviewed
• [Journal Article] Suppression of pleiotrophic phenotypes of Burkholderia multivorans fur mutant by oxyR mutation (2012)
  • Author(s): A.Kimura
  • Journal Title: Microbiology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Pivotal role of anthranilate dioxygenase genes in the adaptation of Burkholderia multivorans ATCC 17616 in soil (2012)
  • Author(s): E.Nishiyama
  • Journal Title: FEMS Microbiol.Lett. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Genomic organization and genomic structural rearrangements of Sphingobium japonicum UT26, an archetypalgamma-hexachlorocyclohexane-degrading bacterium (2011)
  • Author(s): Y. Nagata, 3人, Y. Ohtsubo, 5人, and M. Tsuda
  • Journal Title: Enzyme Microb. Technol Volume: 49 Pages: 499-508
  • Peer Reviewed
• [Journal Article] Ehrlich, and Bork: Enterotypes of the human gut microbiome (2011)
  • Author(s): M. Arumugam, 18人, K. Kurokawa, 19人, MetaHIT Consortium, J. Weissenbach, S. D
  • Journal Title: Nature Volume: 473 Pages: 174-180
  • Peer Reviewed
• [Journal Article] Enterotypes of the human gut microbiome (2011)
  • Author(s): M.Arumugam
  • Journal Title: Nature Volume: 473 Pages: 174-180
  • Peer Reviewed
• [Journal Article] Genomic organization and genomic structural rearrangements of Sphingobium japonicum UT26, an archetypal gamma-hexachlorocyclohexane-degrading bacterium (2011)
  • Author(s): Y.Nagata
  • Journal Title: Enzyme and Microbial Technology Volume: 49 Pages: 499-508
  • Peer Reviewed
• [Journal Article] CRISPR inhibition of prophage acquisition in Streptococcus pyogenes (2011)
  • Author(s): T.Nozawa
  • Journal Title: PLoS One Volume: 6 Issue: 5 Pages: e19543-e19543
  • DOI: 10.1371/journal.pone.0019543
  • Peer Reviewed
• [Journal Article] The lifestyle of the segmented filamentous bacterium : a non-culturable gut-associated immunostimulating microbe inferred by whole-genome sequencing (2011)
  • Author(s): T.Kuwahara
  • Journal Title: DNA Res Volume: 18 Pages: 291-301
  • Peer Reviewed
• [Journal Article] The lin genes for γ-hexachlorocyclohexane degradation in Sphingomonas sp.MM-1 proven to be dispersed across multiple plasmids (2011)
  • Author(s): M.Tabata
  • Journal Title: Biosci.Biotech.Biochem. Volume: 75 Pages: 466-472
  • Peer Reviewed
• [Journal Article] Identification of Burkholderia multivorans ATCC 17616 genes induced insoil environment by in vivo expression technology (2010)
  • Author(s): E. Nishiyama, Y. Ohtsubo, Y. Nagata, and M. Tsuda
  • Journal Title: Environ. Microbiol Volume: 12 Pages: 2539-2558
  • Peer Reviewed
• [Journal Article] VITCOMIC: visualization tool for taxonomic compositions of microbial communities based on 16S rRNA gene sequences (2010)
  • Author(s): H. Mori, F. Maruyama and K. Kurokawa
  • Journal Title: BMC Bioinfo Volume: 11 Issue: 1 Pages: 332-332
  • DOI: 10.1186/1471-2105-11-332
  • Peer Reviewed
• [Journal Article] Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 catabolic plasmids (2010)
  • Author(s): H. Yano, 3人, Y. Nagata, M. Hattori, M. Tsuda
  • Journal Title: J. Bacteriol Volume: 192 Pages: 4337-4347
  • Peer Reviewed
• [Journal Article] 芳香族化合物による土壌撹乱における微生物遺伝子プールの変動 (2010)
  • Author(s): 加藤広海,大坪嘉行,永田裕二,津田雅孝
  • Journal Title: J.Environ. Biotechnol Volume: 10 Pages: 63-70
  • Peer Reviewed
• [Journal Article] ゲノム解析ソフトウエアGenomeMatcher (2010)
  • Author(s): 大坪嘉行,大坪和香子,永田裕二,津田雅孝
  • Journal Title: 化学と生物 Volume: 48 Pages: 313-319
  • NAID: 10026438311
  • Peer Reviewed
• [Journal Article] Identification of Burkholderia multivorans ATCC 17616 genes induced in soil environment by in vivo expression technology (2010)
  • Author(s): E.Nishiyama
  • Journal Title: Environ.Microbiol. Volume: 12 Pages: 2539-2558
  • Peer Reviewed
• [Journal Article] Complete nucleotide sequence of TOL plasmid pDK1 provides evidence for evolutionary history of IncP-7 calabolic plasmids (2010)
  • Author(s): H.Yano
  • Journal Title: J.Bacteriol. Volume: 192 Pages: 4337-4347
  • Peer Reviewed
• [Journal Article] Complete genome sequence of the representative γ-hexachlorocyclohexane-degrading bacterium Sphingobium japonicum UT26 (2010)
  • Author(s): Y.Nagata
  • Journal Title: J.Bacteriol. Volume: 192 Pages: 5852-5853
  • Peer Reviewed
• [Journal Article] VITCOMIC : visualization tool for taxonomic compositions of microbial communities based on 16S rRNA gene sequences (2010)
  • Author(s): H.Mori
  • Journal Title: BMC Bioinfo Volume: 11 Pages: 332-332
  • Peer Reviewed
• [Journal Article] ゲノム解析ソフトウェアGenomeMatcherの紹介 (2010)
  • Author(s): 大坪嘉行
  • Journal Title: 化学と生物 Volume: 48 Pages: 313-318
  • Peer Reviewed
• [Journal Article] 芳香族化合物による土壌撹乱における微生物遺伝子プールの変動 (2010)
  • Author(s): 加藤広海
  • Journal Title: J.Environ.Biotechnol. Volume: 10 Pages: 63-71
  • Peer Reviewed
• [Journal Article] 機能相補による芳香族化合物複合汚染土壌からの新規分解酵素遺伝子の探索
  • Author(s): 永山浩史,菅原智詞,遠藤諒,加藤広海,大坪嘉行,永田裕二,津田雅孝
  • Journal Title: J.Environ. Biotechnol Volume: (in press)
  • Peer Reviewed
• [Presentation] 環境メタゲノム解析における微生物種 (2013)
  • Author(s): 津田雅孝
  • Organizer: 日本農芸化学会2013年大会
  • Place of Presentation: 仙台
• [Presentation] Time series metagenomic analysis of soil microbial community (2013)
  • Author(s): M. Tsuda and K. Kurokawa
  • Organizer: International Symposium on Genome Science "Expanding Frontiers of Genome Science"
  • Place of Presentation: Tokyo
• [Presentation] メタゲノム解析の現状と将来 (2013)
  • Author(s): 黒川顕
  • Organizer: 社団法人企業研究会CAMMフォーラム
  • Place of Presentation: 東京
  • Invited
• [Presentation] リダクターゼ機能相補による新規芳香族化合物分解酵素遺伝子の芳香族化合物複合汚染土壌メタゲノムからの探索 (2013)
  • Author(s): 永山浩史
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜
• [Presentation] 微生物統合データベースMicrobeDB.jp (2013)
  • Author(s): 森宙史
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜
• [Presentation] 撹乱に対する土壌微生物のレジリエンス (2013)
  • Author(s): 加藤広海
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜
• [Presentation] 芳香族化合物複合汚染土壌における新規芳香族化合物分解酵素遺伝子の機能相補に基づく探索 (2013)
  • Author(s): 永山浩史
  • Organizer: 日本農芸化学会2013年大会
  • Place of Presentation: 仙台
• [Presentation] Deciphering ancient ecosystems with environmental genomics and “Earth-Life DB” (2013)
  • Author(s): Ken Kurokawa
  • Organizer: The 1st ELSI International Symposium
  • Place of Presentation: Tokyo
  • Invited
• [Presentation] メタゲノミクスの現状と未来 (2012)
  • Author(s): 黒川顕
  • Organizer: 情報・システム研究機構シンポジウム2012「生命科学のビッグデータ革命」
  • Place of Presentation: 東京
  • Year and Date: 2012-11-09
• [Presentation] Development of an integrated analysis system for metagenomics and a global microbial database MicrobeDB.jp (2012)
  • Author(s): K. Kurokawa
  • Organizer: 第28回日本微生物生態学会
  • Place of Presentation: 豊橋
  • Year and Date: 2012-09-20
• [Presentation] Response of soil microbial community after exposure to aromatic pollutants (2012)
  • Author(s): 津田雅孝
  • Organizer: 環境保全と健常生活のための先端バイオテクノロジーの統合的研究第3回公開シンポジウム
  • Place of Presentation: 多賀城(招待講演)
  • Year and Date: 2012-03-13
• [Presentation] Development of an integrated analysis system for metagenomics and its applications for soil metagenome analysis (2012)
  • Author(s): K.Kurokawa
  • Organizer: BiWO2011
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2012-01-26
• [Presentation] Metagenomic Analysis of Microbial Community in Soil Contaminated with Aromatic Pollutants (2012)
  • Author(s): M. Tsuda, 5 人, Y. Ohtsubo, Y. Nagata, A. Fujiyama, and K. Kurokawa
  • Organizer: Japan-Finland Biotechnology Symposium2012
  • Place of Presentation: Sendai
• [Presentation] 環境メタゲノミクス (2012)
  • Author(s): 黒川顕
  • Organizer: 日本農芸化学会2011年大会
  • Place of Presentation: 京都
• [Presentation] 環境汚染物質添加土壌での微生物集団の経時的メタゲノム解析 (2012)
  • Author(s): 津田雅孝
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] Metagenomic Analysis of Microbial Community in Soil Contaminated with Aromatic Pollutants (2012)
  • Author(s): Masataka Tsuda
  • Organizer: Japan-Finland Biotechnology Symposium 2012
  • Place of Presentation: 仙台
  • Invited
• [Presentation] 培養非依存的手法を用いた芳香族化合物複合汚染土壌からの新規分解酵素遺伝子の探索 (2012)
  • Author(s): 永山浩史
  • Organizer: 環境バイオテクノロジー学会2012年度大会
  • Place of Presentation: 宇治
• [Presentation] Response of soil microbial community against pulse perturbation by aromatic pollutants (2012)
  • Author(s): Hiromi Kato
  • Organizer: 14th International Symposium on Microbial Ecology
  • Place of Presentation: Copenhagen, Denmark
• [Presentation] Isolation of aromatic ring-hydroxylating oxygenase genes by cultivation-independent functional screening of metagenome from soil artificially contaminated with aromatic hydrocarbons (2012)
  • Author(s): Hirofumi Nagayama
  • Organizer: 15th International Biotechnology Symposium and Exhibition
  • Place of Presentation: Daegu, Korea
• [Presentation] 環境汚染物質添加土壌での微生物集団の経時的メタゲノム解析 (2012)
  • Author(s): 津田雅孝
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 東京(招待講演)
• [Presentation] 土壌攪乱に対し同調して動く遺伝子群の解析 (2012)
  • Author(s): 加藤広海
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] 土壌細菌群集団の移植と芳香族化合物による汚染化の変動解析 (2012)
  • Author(s): 永山浩史
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] 細菌群集に環境刺激に対する頑健性を与える要因の解明 (2012)
  • Author(s): 伊藤貴広
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] 環境メタゲノミクス (2012)
  • Author(s): 黒川顕
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都(招待講演)
• [Presentation] 環境汚染物質分解能を担うプラスミドの水平伝達 (2012)
  • Author(s): 津田雅孝
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都(招待講演)
• [Presentation] フィニッシング用のツールGenoFinisherの開発 (2012)
  • Author(s): 大坪嘉行
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
• [Presentation] 芳香族化合物汚染土壌における経時的メタゲノムによって明らかになった土壌微生物の適応反応 (2012)
  • Author(s): 加藤広海
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
• [Presentation] 土壌細菌Burkholderia multivorans ATCC 17616株の鉄応答転写因子Furと過酸化水素応答転写因子OxyRの各種表現型への関与 (2012)
  • Author(s): 木村明音
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
• [Presentation] 培養非依存的手法による土壌からの新規芳香族化合物分解遺伝子の探索 (2012)
  • Author(s): 永山浩史
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
• [Presentation] STM法で同定した土壌細菌Burkholderia multivorans ATCC 17616株の土壌環境下での生残に重要な遺伝子の解析 (2012)
  • Author(s): 石橋蓉子
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
• [Presentation] 微生物ドラフトゲノム配列のフィニッシング用ツール:GenoFinisher (2012)
  • Author(s): 大坪嘉行
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎(招待講演)
• [Presentation] 生態系の理解に向けたゲノム情報の解析と活用 (2011)
  • Author(s): 黒川顕
  • Organizer: 農業環境技術研究所公開セミナー
  • Place of Presentation: つくば(招待講演)
  • Year and Date: 2011-12-06
• [Presentation] ゲノムからメタゲノムへ (2011)
  • Author(s): 黒川顕
  • Organizer: CBI/JSBi2011合同大会
  • Place of Presentation: 神戸(招待講演)
  • Year and Date: 2011-11-09
• [Presentation] 新型シーケンサーによるメタゲノム解析 (2011)
  • Author(s): 黒川顕
  • Organizer: 第63回生物工学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-27
• [Presentation] 新型シーケンサーによるメタゲノム解析 (2011)
  • Author(s): 黒川顕
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-09-27
• [Presentation] 環境変動に対する土壌微生物集団応答様式のメタゲノム的解析 (2011)
  • Author(s): 津田雅孝
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都
• [Presentation] Response of soil microbiota to exposure to aromatic pollutants (2011)
  • Author(s): M. Tsuda
  • Organizer: International Union of Microbiological Societies 2011Congress
  • Place of Presentation: Sapporo
• [Presentation] 微生物ゲノムFinishing支援ソフトウエア:GenoFinisher (2011)
  • Author(s): 大坪嘉行
  • Organizer: ゲノム微生物学会ワークショップ
  • Place of Presentation: 仙台
• [Presentation] 土壌メタゲノムから見る,芳香族化合物汚染により誘導される微生物群集の反応 (2011)
  • Author(s): 加藤広海
  • Organizer: ゲノム微生物学会ワークショップ
  • Place of Presentation: 仙台
• [Presentation] 芳香族化合物複合汚染土壌由来のメタゲノムライブラリからの機能相補による新規リダクターゼ遺伝子の探索 (2011)
  • Author(s): 永山浩史
  • Organizer: ゲノム微生物学会ワークショップ
  • Place of Presentation: 仙台
• [Presentation] Response of soil microbiota to exposure to aromatic pollutants (2011)
  • Author(s): M.Tsuda
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan(招待講演)
• [Presentation] Fluctuation pattern of gene pools of microbiota in aromatic hydrocarbon-contaminated soil by sequential metagenomic analysis (2011)
  • Author(s): H.Kato
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] Alleviation of nitric oxide sensitivity of Burkholderia multivorans ATCC 17616 fur mutant by additional mutation in oxyR gene (2011)
  • Author(s): A.Kimura
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] Development of a computational tool for finishing of draft genomic sequence (2011)
  • Author(s): Y.Ohtsubo
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] 環境変動に対する土壌微生物集団応答様式のメタゲノム的解析 (2011)
  • Author(s): 津田雅孝
  • Organizer: 遺伝学会第83回大会
  • Place of Presentation: 京都(招待講演)
• [Presentation] Direct isolation and characterization of genes for degradation of aromatic compounds from soils that were artificially contaminated with aromatic pollutants (2011)
  • Author(s): H.Nagayama
  • Organizer: International Forum for Ecosystem Adaptability Science III
  • Place of Presentation: Sendai, Japan
• [Presentation] 土壌細菌Burkholderia multivorans ATCC 17616株のFurとOxyRの遺伝学的関連とメカニズムの解明 (2011)
  • Author(s): 木村明音
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台
• [Presentation] 機能ベースのメタゲノム的手法で取得した芳香族化合物代謝関連遺伝子群の解析 (2011)
  • Author(s): 菅原智詞
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台
• [Presentation] 土壌細菌Burkholderia multivorans ATCC17616株の土壌環境中における特異的発現遺伝子の解析 (2011)
  • Author(s): 三浦那智
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台
• [Presentation] 土壌細菌Burkholderia multivorans ATCC 17616株の土壌環境下で生育に重要な遺伝子の探索・解析 (2011)
  • Author(s): 千本木淳子
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台
• [Presentation] 芳香族化合物汚染により誘導される土壌遺伝子プールの変動解析 (2011)
  • Author(s): 加藤広海
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台
• [Presentation] 土壌汚染により優先したBurkholderia群集の系統と代謝能力の遷移 (2011)
  • Author(s): 熊野知拡
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台
• [Presentation] 微生物ゲノム完全長決定支援ソフトウエア454Finisher (2011)
  • Author(s): 大坪嘉行
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台
• [Presentation] Burkholderia multivorans ATCC 17616株の鉄応答転写因子Furと過酸化水素応答転写因子OxyRの遺伝学的関連性の解明 (2011)
  • Author(s): 木村明音
  • Organizer: 日本農芸化学会2011年大会
  • Place of Presentation: 京都
• [Presentation] 土壌細菌Burkholderia multivorans ATCC17616株の土壌環境下で生育に重要な遺伝子の同定 (2011)
  • Author(s): 千本木淳子
  • Organizer: 日本農芸化学会2011年大会
  • Place of Presentation: 京都
• [Presentation] 土壌細菌Burkholderia multivorans ATCC17616株の土壌特異的発現遺伝子の解析 (2011)
  • Author(s): 三浦那智
  • Organizer: 日本農芸化学会2011年大会
  • Place of Presentation: 京都
• [Presentation] 土壌の汚染化によって起こる微生物遺伝子プールの変動 (2011)
  • Author(s): 加藤広海
  • Organizer: 日本農芸化学会2011年大会
  • Place of Presentation: 京都
• [Presentation] 土壌汚染によって優先したBurkholderia群集の系統および代謝能力の遷移 (2011)
  • Author(s): 熊野知拡
  • Organizer: 日本農芸化学会2011年大会
  • Place of Presentation: 京都
• [Presentation] 培養非依存的手法で取得した芳香族化合物代謝関連遺伝子群の解析 (2011)
  • Author(s): 菅原智詞
  • Organizer: 日本農芸化学会2011年大会
  • Place of Presentation: 京都
• [Presentation] ドラフトゲノム配列のFinishing支援ツール:454Finisher (2011)
  • Author(s): 大坪嘉行
  • Organizer: 日本農芸化学会2011年大会
  • Place of Presentation: 京都
• [Presentation] Fluctuation of gene pools of soil microbiota induced by contamination with aromatic hydrocarbons (2010)
  • Author(s): H. Kato, 2 人, Y. Ohtsubo, 5 人, Y. Nagata, A. Fujiyama, K. Kurokawa and M. Tsuda
  • Organizer: 13th International Society for Microbial Ecology Conference
  • Place of Presentation: Seattle, USA
• [Presentation] Meta-plasmid: In silico reconstruction of a plasmid-like circular DNA molecule and its possible role in the retrieved environment (2010)
  • Author(s): H. Suenaga, 5 人, Y. Ohtsubo, M. Tsuda, and K. Miyazaki
  • Organizer: 13th International Society for Microbial Ecology Conference
  • Place of Presentation: Seattle, USA
• [Presentation] 土壌汚染に対する微生物遺伝子プールの変動解析 (2010)
  • Author(s): 津田雅孝,加藤広海,大坪嘉行,永田裕二
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 仙台
• [Presentation] 第二世代シークエンサを用いた、土壌汚染に伴う微生物の適応反応の解析 (2010)
  • Author(s): 加藤広海
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 仙台
• [Presentation] Burkholderia multivorans ATCC 17616株の土壌特異的発現遺伝子の同定と解析 (2010)
  • Author(s): 西山依里
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 仙台
• [Presentation] 土壌細菌Burkholderia multivoransにおける鉄応答転写因子と過酸化水素応答転写因子の関連 (2010)
  • Author(s): 木村明音
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 仙台
• [Presentation] メタゲノムアプローチにより多重汚染土壌から取得した芳香族化合物初発酸化酵素遺伝子の解析 (2010)
  • Author(s): 菅原智詞
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 仙台
• [Presentation] STM法による土壌細菌Burkholderia multivorans ATCC 17616株の土壌環境下で生育に重要な遺伝子の探索 (2010)
  • Author(s): 千本木淳子
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 仙台
• [Presentation] 解析ソフトウエアGenomeMatcherのドラフトゲノムシーケンスのコンプリート支援機能 (2010)
  • Author(s): 大坪嘉行
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 仙台
• [Presentation] 芳香族化合物分解系プラスミドの安定的維持を阻害する宿主因子 (2010)
  • Author(s): 宮腰昌利
  • Organizer: 環境バイオテクノロジー学会2010年度大会
  • Place of Presentation: 仙台
• [Presentation] Meta-plasmid : In silico reconstruction of a plasmid-like circular DNA molecule and its possible role in the retrieved environment (2010)
  • Author(s): H.Suenaga
  • Organizer: 13th International Society for Microbial Ecology Conference
  • Place of Presentation: Seattle, USA
• [Presentation] Genetic interaction of global transcriptional regulators, Fur and OxyR, in Burkholderia multivorans (2010)
  • Author(s): A.Kimura
  • Organizer: 14th International Biotechnology Symposium
  • Place of Presentation: Rimini, Italy
• [Presentation] PCB/biphenyl degradation operon repressed by different compounds in Acidovorax sp.KKS102 and Burkholderia multivorans ATCC 17616 (2010)
  • Author(s): Y.Ohtsubo
  • Organizer: 14th International Biotechnology Symposium
  • Place of Presentation: Rimini, Italy
• [Presentation] Burkholderia multivorans ATCC17616株の芳香族化合物トランスポーター様遺伝子の解析 (2010)
  • Author(s): 小川直人
  • Organizer: 第26回日本微生物生態学会
  • Place of Presentation: つくば
• [Presentation] 芳香族化合物汚染により誘導される土壌メタゲノムの変動様式とそのメカニズム (2010)
  • Author(s): 加藤広海
  • Organizer: 第26回日本微生物生態学会
  • Place of Presentation: つくば
• [Book] Biodegradative Bacteria: How Bacteria Degrade, Survive, Adapt, and Evolve (2013)
  • Author(s): Yoshiyuki Ohtsubo
  • Publisher: Springer Verlag
• [Book] メタゲノムインフォマティクス,難培養微生物研究の最新技術II (2010)
  • Author(s): 森宙史,丸山史人,黒川顕
  • Publisher: シーエムシー出版,東京.
• [Book] メタゲノムデータベース,メタゲノム解析技術の最前線 (2010)
  • Author(s): 森宙史,丸山史人,黒川顕
  • Publisher: シーエムシー出版,東京.
• [Book] 環境汚染物質分解細菌のメタゲノミクス「難培養性微生物研究の最新技術II」(大熊盛也,工藤俊章編集) (2010)
  • Author(s): 永田裕二,津田雅孝
  • Publisher: シーエムシー出版
• [Book] 酵素利用技術大系 (2010)
  • Author(s): 永田裕二
  • Publisher: 株)エヌ・ティー・エス
• [Book] 難培養微生物研究の最新技術II (2010)
  • Author(s): 森宙史
  • Publisher: シーエムシー出版
• [Book] 難培養微生物研究の最新技術II (2010)
  • Author(s): 永田裕二
  • Publisher: シーエムシー出版
• [Book] メタゲノム解析技術の最前線 (2010)
  • Author(s): 森宙史
  • Publisher: シーエムシー出版
• [Book] Strategies to reveal genomic function in natural soil systems In:Nojiri, H., M. Tsuda, M. Fukuda, and Y. Kamagata (eds)
  • Author(s): Y. Ohtsubo, E. Nishiyama, Y. Ishibashi, Y. Nagata, and M. Tsuda
  • Publisher: Biodegradative Bacteria. Springer Verlag, Tokyo