Functional analysis of plant resistant gene markers expressed by microbial community

• Project/Area Number: 22380177
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Gifu University
• Principal Investigator: HYAKUMACHI Mitsuro 岐阜大学, 応用生物科学部, 教授 (90113612)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Yoshiharu 岐阜大学, 応用生物科学部, 教授 (50301784) ; 小山 博之 岐阜大学, 応用生物科学部, 教授 (90234921)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 発病抑制 / 抵抗性誘導 / 組み合わせ接種 / 遺伝子マーカー / 植物生育促進菌類 / 揮発性成分 / SA/H2O2応答 / ABA応答 / 発病抑止土壌 / 拮抗微生物 / 土壌微生物相 / PCR-DGGE解析 / 発病衰退現象

Research Abstract

The co-inoculation of plant growth-promoting fungi(PGPF) and arbuscular mycorrhizal fungi (AMF) resulted in additive effect on the suppression of anthracnose disease.The co-inoculation of PGPF and AMF also resulted in effective control of Cucumber mosaic virus disease severity.Two volatile organic compounds emitted fro PGPF significantly reduced disease severity in Arabidopsis plants against Pseudomonas syringae pv. tomato DC3000.

Research Products

• [Journal Article] Systemic resistance induced by volatile organic compounds emitted by plant growth-promoting fungi in Arabidopsis thaliana (2014)
  • Author(s): Naznin, H.A., Kiyohara, D., Kimura, M., Miyazawa, M., Shimizu, M., and Hyakumachi, M.
  • Journal Title: PLoS ONE Volume: (in press) Issue: 1 Pages: 1-10
  • DOI: 10.1371/journal.pone.0086882
  • Peer Reviewed
• [Journal Article] Analysis of Volatile Organic Compounds Emitted by Plant Growth-Promoting Fungus <i>Phoma</i> sp. GS8-3 for Growth Promotion Effects on Tobacco (2013)
  • Author(s): Naznin, H.A., Kimura, M., Miyazawa, M., and Hyakumachi, M.
  • Journal Title: Microbes and Environments Volume: 28 Issue: 1 Pages: 42-49
  • DOI: 10.1264/jsme2.ME12085
  • NAID: 10031138542
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] Induction of systemic resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1 (2013)
  • Author(s): Elsharkawy, M.M., Shimizu, M., Takahashi, H., Ozaki, K., and Hyakumachi, M.
  • Journal Title: Plant Pathol J Volume: 29 Issue: 2 Pages: 193-200
  • DOI: 10.5423/ppj.si.07.2012.0117
  • Peer Reviewed
• [Journal Article] <i>Bacillus thuringiensis</i> Suppresses Bacterial wilt Disease Caused by <i>Ralstonia solanacearum</i> with Systemic Induction of Defense-Related Gene Expression in Tomato (2013)
  • Author(s): Hyakumachi, M., Nishimura, M., Arakawa, T., Asano, S., Yoshida, S., Tsushima, S. and Takahashi, H.
  • Journal Title: Microbes and Environments Volume: 28 Issue: 1 Pages: 128-134
  • DOI: 10.1264/jsme2.ME12162
  • NAID: 10031138553
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] The plant growth-promoting fungus Fusarium equiseti and the arbuscular mycorrhizal fungus Glomus mosseae induce systemic resistance against Cucumuber mosaic virus in cucumber plants (2012)
  • Author(s): Elsharkawy, M.M., Shimizu, M., Takahashi, H., and Hyakumachi, M.
  • Journal Title: Plant Soil Volume: 361 Issue: 1-2 Pages: 397-409
  • DOI: 10.1007/s11104-012-1255-y
  • Peer Reviewed
• [Journal Article] Induction of systemic resistance against Cucumber mosaic virus by Penicillium simplicissimum GP17-2 in Arabidopsis and tobacco (2012)
  • Author(s): Elsharkawy M. M., Shimizu M., Takahashi H. and Hyakumachi M.
  • Journal Title: Plant Pathology Volume: 61 Issue: 5 Pages: 964-976
  • DOI: 10.1111/j.1365-3059.2011.02573.x
  • Peer Reviewed
• [Journal Article] Interaction between the plant growth-promoting fungus Phoma sp. GS8-2 and the arbuscular mycorrhizal fungus Glomus mosseae: impact on biocontrol of soil-borne diseases, microbial population, and plant growth. (2012)
  • Author(s): M.G.B. Saldajeno
  • Journal Title: Australasian Plant Pathol. Volume: 41 Issue: 3 Pages: 271-281
  • DOI: 10.1007/s13313-011-0101-7
  • Peer Reviewed
• [Journal Article] Fusarium azukicola sp. nov., an exotic azuki bean root-rot pathogen in Hokkaido, Japan (2012)
  • Author(s): T. Aoki
  • Journal Title: Mycologia Volume: 104 Issue: 5 Pages: 1068-1084
  • DOI: 10.3852/11-303
  • Peer Reviewed
• [Journal Article] The plant growth-promoting fungus Fusarium equiseti and the arbuscular mycorrhizal fungus Glomus mosseae stimulate plant growth and reduce severity of anthracnose and damping-off diseases in cucumber (Cucumis sativus)seedlings (2011)
  • Author(s): Saldajeno, M.G.B., Hyakumachi, M.
  • Journal Title: Ann.Appl.Biol. Volume: 159 Pages: 28-40
  • Peer Reviewed
• [Journal Article] Biological control of Fusarium wilt of tomato with Fusarium equiseti GF191 in both rock wool and soil systems (2011)
  • Author(s): Horinouchi, H., Watanabe, H., Taguchi, Y., Muslim, A., Hyakumachi, M.
  • Journal Title: BioControl Volume: 56 Pages: 915-923
  • Peer Reviewed
• [Journal Article] Plant growth-promoting fungus, Trichoderma koningi suppresses isoflavonoid phytoalexin vestitol production for colonization on/in the roots of Lotus japonicus (2011)
  • Author(s): Masunaka, A., Hyakumachi, M., Takenaka, S.
  • Journal Title: Microbes Environ. Volume: 26 Pages: 128-134
  • NAID: 10029122750
  • Peer Reviewed
• [Journal Article] BIOCONTROL OF FUSARIUM WILT OF SPINACH BY THE PLANTGROWTH PROMOTING FUNGUS FUSARIUM EQUISETI GF183 (2010)
  • Author(s): H.Horinouchi, A.Muslim, M.Hyakumachi
  • Journal Title: Journal of Plant Pathology Volume: 92(1) Pages: 249-254
  • Peer Reviewed
• [Presentation] In silico analysis of transcriptional regulatory elements related with disease resistance (2012)
  • Author(s): Hushna Ara Naznin, Yoshioka, Y., Hieno, A., Hyakumachi, M. and Yamamoto, Y
  • Organizer: In the program and abstract brochure of the 15th International congress on molecular plant microbe interaction (IS-MPMI) at Koyoto, Japan
  • Place of Presentation: Poster presentation
• [Book] Enhanced resistance of plants to disease using Trichoderma spp. In Biotechnology and Biology of Trichoderma (2014)
  • Author(s): Saldajeno, M.G.S., Naznin, H.A., Elsharkawy, M.M., Shimizu, M. and Hyakumachi, M
  • Publisher: Elsevier, Amsterdam
• [Book] Ｂｉｏｔｅｃｈｎｏｋｌｏｇｙ ａｎｄ Ｂｉｏｌｏｇｙ ｏｆ Ｔｒｉｃｈｏｄｅｒｍａ (2014)
  • Author(s): Saldajeno, M.G.B., Naznin, H.A., Elsharkawy, M.M., Shimizu, M. and Hyakumachi, M.
  • Total Pages: 549
  • Publisher: Elsevier
• [Book] Arbuscular mycorrhizal interactions with rhizobacteria or saprotrophic fungi and its implications to biological control of plant diseases(In Mycorrhizal Fungi (ed. by Fulton, S.M.)) (2011)
  • Author(s): Saldajeno, M.G.B and Hyakumachi, M.
  • Publisher: Nova Science Publishers, Inc.