| Project/Area Number |
22390005
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
AOKI Shin 東京理科大学, 薬学部生命創薬科学科, 教授 (00222472)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Ryo 東京理科大学, 生命医科学研究所, 教授 (20159453)
HAYASE Masanori 東京理科大学, 理工学部, 准教授 (70293058)
NAKATSURA Tetsuya 国立がん研究センター東病院, 臨床開発センター, 機能再生室長 (30343354)
ITOH Masaaki 国立がん研究センター東病院, 臨床開発センター, 消化器科医長 (40312144)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
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| Keywords | 血中循環腫瘍細胞 / 光分解反応 / がん診断 / シリコン基板 / 抗体 |
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| Research Abstract |
Circulating tumor cells (CTCs) have been defined as cancer cells of solid tumor origin found in the peripheral blood. It isgenerally considered that these CTCs detach from primary tumors of patients, go into the bloodstream, travel around in the body, and then attach to the remote tissues. Accordingly, it is generally described that the quantitative detection and analysis of CTC is considered to be useful for the personal diagnosis of cancer patients. In this work, a device for the capture and recollection of live target cells is developed The platform was a silicon (Si) wafer modified with an anti-HEL antibody (anti-HEL-IgG, HEL= hen egg lysozyme) through a photocleavable 3-amino-3-(2-nitrophenyl)propionic acid (ANP) linker. The modification processes of the Si wafer surface were monitored by Fourier transform infrared spectroscopy by attenuated total reflection (FTIR-ATR) and fast-scanning atomic force microscopy (FS-AFM). The attachment of IgG and its release reaction on the Si surface via the photochemical cleavage of the ANP linker were observed by FS-AFM and an enzyme-linked immunosorbent assay (ELISA). Furthermore, it was possible to selectively collect SP2/O cells that express HEL on their cell membranes (SP2/O-HEL) on the Si wafer device. Photochemical cleavage of the ANP linker facilitated the effective release of living SP2/O cells, whose viability was verified by staining experiments using tripan-blue. Moreover, it was possible to re-culture the recovered cells. This methodology represents an effective strategy for isolating intact target cells in the biological and medicinal sciences and related fields.
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