|Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Laminins are a major component of the basement membrane, a thin extracellular matrix, and have diverse biological activities. Our goal is to identify active sequences from laminins and to use the biologically active peptides for medical application including anti-cancer and wound healing drugs, DDS, and biomaterials. We have identified various biologically active peptides using more than 1000 synthetic peptides derived from the laminins. We also identified cellular receptors of the active peptides and evaluated their cellular signaling. We also demonstrated that peptide-conjugated polysaccharide (chitosan, alginate, and agarose) matrices are useful as a biomaterial for cell culture and tissue engineering. Further, mixed peptide-matrices using several types of receptor-binding peptides promoted synergistic effects. Using a combination of tissue-appropriate laminin-derived peptides, the mixed peptide-polysaccharide matrices may serve as a functional biomaterial for tissue engineering.