| Project/Area Number |
22390033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO Chizuru 千葉大学, 大学院・医学研究院, 講師 (80347054)
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| Co-Investigator(Renkei-kenkyūsha) |
MAEKAWA Mamiko 千葉大学, 大学院・医学研究院, 助教 (20181571)
YAMATOYA Kenji 千葉大学, 大学院・医学研究院, 特任研究員 (80447309)
KAMIMURA Kyoko 千葉大学, 大学院・医学研究院, 技術専門職員 (20422264)
MUTOH Tohru 千葉大学, 大学院・医学研究院, 技術専門職員 (30422265)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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| Keywords | 精子 / 受精 / エクアトリン / ライブセルイメージング / 遺伝子改変マウス / Equatorin / 不妊症 / 先体反応 / 精子形成 / 胚発生 / 不妊 / 受精卵 / Izumo / CD9 / 卵活性 / 卵子 |
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| Research Abstract |
We have established Eqtn-EGFP-TG mouse and ODF2-EGFP-TG mouse lines, and tried to take live-image of the molecular change from the acrosome reaction throughout to egg activation. A whole cell imaging (80-100nm acrosomal membrane complex) of Eqtn-EGFP-TG sperm was obtained by new microscopy STED system without sectioning, which suggested a possibility of live cell imaging during fertilization. Galnt3 transferase is required for O-glycosylation of carbohydrate chain branching from threonine 138 of equatorin molecule. We clarified the distribution abnormality of egg activation related MN13 molecule in the infertile patient who have round-head sperm (globozoospermia), by comparing the expression with that of the model mouse GOPC-/- spermatozoa. Based on these analyses, we proposed the mechanism leading to egg activation failure in human sperm.
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