Cellular and molecular study on live imaging of expression of sperm functional proteins and its relation to infertility
| Project/Area Number |
22390033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO Chizuru 千葉大学, 大学院・医学研究院, 講師 (80347054)
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| Co-Investigator(Renkei-kenkyūsha) |
MAEKAWA Mamiko 千葉大学, 大学院・医学研究院, 助教 (20181571)
YAMATOYA Kenji 千葉大学, 大学院・医学研究院, 特任研究員 (80447309)
KAMIMURA Kyoko 千葉大学, 大学院・医学研究院, 技術専門職員 (20422264)
MUTOH Tohru 千葉大学, 大学院・医学研究院, 技術専門職員 (30422265)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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| Keywords | 精子 / 受精 / エクアトリン / ライブセルイメージング / 遺伝子改変マウス / Equatorin / 不妊症 / 先体反応 / 精子形成 / 胚発生 / 不妊 / 受精卵 / Izumo / CD9 / 卵活性 / 卵子 |
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| Research Abstract |
We have established Eqtn-EGFP-TG mouse and ODF2-EGFP-TG mouse lines, and tried to take live-image of the molecular change from the acrosome reaction throughout to egg activation. A whole cell imaging (80-100nm acrosomal membrane complex) of Eqtn-EGFP-TG sperm was obtained by new microscopy STED system without sectioning, which suggested a possibility of live cell imaging during fertilization. Galnt3 transferase is required for O-glycosylation of carbohydrate chain branching from threonine 138 of equatorin molecule. We clarified the distribution abnormality of egg activation related MN13 molecule in the infertile patient who have round-head sperm (globozoospermia), by comparing the expression with that of the model mouse GOPC-/- spermatozoa. Based on these analyses, we proposed the mechanism leading to egg activation failure in human sperm.
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Report
(4 results)
Research Products
(154 results)
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[Journal Article] Tysndl deficiency in mice interferes with the peroxisomal location of PTS2 enzymes2013
Author(s)
Mizuno Y, Ninomiya Y, Nakachi Y, Iseki M, Iwasa H, Akita M, Tsukui T, ShimozawaN, Ito C, Toshimori K, Nishimura M, Hara H, Maeba R, Okazaki T, Alodaib AN, Amoudi MA,Jacob M, Alkuraya FS, Horai Y, Watanabe M, Motegi H, Wakana S, Noda T, Kurochkin IV, Mizuno Y, Schonbach C, Okazaki Y
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Journal Title
causinglipid metabolic abnormalities and maleinfertility. PLoS genet
Volume: 9(2)
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Peer Reviewed
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[Journal Article] Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage2011
Author(s)
Sato B, Katagiri YU, Miyado K, Okino N, Ito M, Akutsu H, Okita H, Umezawa A, Fujimoto J, Toshimori K, Kiyokawa N
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Journal Title
BMC Dev Biol
Volume: 11
Issue: 1
Pages: 22-22
DOI
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Peer Reviewed
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[Presentation] 精子の構造と機能2011
Author(s)
年森清隆
Organizer
第1回生殖医療研究会
Place of Presentation
東京医科大学病院臨床講堂(東京都)
Year and Date
2011-02-06
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[Presentation] 精子の質の検査2010
Author(s)
伊藤千鶴
Organizer
第55回日本生殖医学会総会 学術講演会
Place of Presentation
ホテルクレスト徳島(徳島県)(招待講演)
Year and Date
2010-11-12
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[Presentation] Oocyte-activation ability correlates with presence of perinuclear theca substance MN13,whose organization is associated with acrosomeformation2010
Author(s)
Ito C, Yamatoya K, Yoshida K, Akutsu H, Yao R, Kyono K, Suzuki-Toyota F, Toyama Y, Maekawa M, Noda T, Toshimori K
Organizer
11th International Symposium on Spermatology Okinawa 2010 (Poster)
Place of Presentation
Okinawa convention center, Okinawa (Japan)
Year and Date
2010-06-25
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[Presentation] Further characterization of acrosomal protein Equatorin2010
Author(s)
Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Araki Y, Miyado K, Toyama Y, Suzuki-Toyoda F, Toshimori K
Organizer
11th International Symposium on Spermatology Okinawa 2010 (Poster)
Place of Presentation
Okinawa convention center, Okinawa (Japan)
Year and Date
2010-06-25
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[Book] 卵子学2011
Author(s)
年森清隆、伊藤千鶴
Total Pages
1210
Publisher
京都大学学術出版
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