| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Outline of Final Research Achievements |
The embryonic liver tissue is autonomously formed by the intracellular interactions among hepatoblasts and other mesenchymal cells. However, the understanding is not sufficient for application in regenerative medicine. In the embryonic mouse liver, TGF-betas are expressed in hepatoblasts and in hepatic mesothelial layer. But, the roles in each cell-lineage have not be analyzed yet. Here, to perform the functional analysis of TGF-beta signaling pathway in hepatoblasts and hepatic mesothelial cell-lineage during the hepatic histogenesis, we conditionally expressed the dominant negative form of human ALK5 in each cell-lineage. In hepatoblasts, forced expression of dnALK5 caused dysgenesis of hepatic epithelia. In hepatic mesothelial cell-lineaege, the forced expression caused dysgenesis of hepatic mesothelium and derivatives. These data indicate that the TGF-beta pathway is involved in branching of hepatoblast cords and maintenance and differentiation of hepatic mesothelial cell-lineage.
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