| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Research Abstract |
We have identified nerve injury induced neuronal endopeptidase (DINE) using peripheral nerve injury model. DINE is induced in bothCNS and PNS neuronal cells in response to nerve injury. To reveal a function of this peptidase, we have made DINE knockout mice. These mice were lethal immediately after birth due to respiration problem. We identified that DINE-deficient mice exhibited the poor innervation of final branching of nerve and only a few neuromuscular junctions (NMJ), suggesting that DINE is a crucial molecule in distal axon arborization into muscle to establish NMJ. To reveal mechanisms underlying the phenotypes, we co-cultured DINE-deficient neurons and Schwann cells, and found that DINE-deficiency affected on axon-Schwann interaction and Schwann maturation. These data suggests that DINE is implicated in maturation of Schwann cells as well as proper construction of NMJ.
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