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Pharmacogenomic study on the drug-sensitive genes using model organisms

Research Project

Project/Area Number 22390048
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionKobe University

Principal Investigator

KUNO Takayoshi  神戸大学, 医学研究科, 特命教授 (50144564)

Co-Investigator(Kenkyū-buntansha) MA Yan  神戸大学, 医学研究科, 講師 (70457050)
MUKAI Hideyuki  神戸大学, バイオシグナル研究センター, 准教授 (80252758)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords免疫抑制薬 / 抗真菌薬 / ストレス応答 / 細胞内情報伝達 / 細胞内輸送 / モデル生物 / スタチン / コレステロール / がん遺伝子 / GPIアンカー / コバルト / マップキナーゼ / アゾール系 / 薬剤耐性 / 薬剤感受性 / 細胞内カルシウム / チャネル / バルプロ酸 / 低分子量GTP結合タンパク質 / グアニンヌクレオチド交換因子 / 酸化ストレス / 転写因子
Research Abstract

In this study we performed many experiments to clarify the molecular mechanisms of adverse action of drugs as well as to overcome the drug resistance. For this purpose we genetically analyzed various fission yeast mutant strains that show hypersensitivity or resistance to various drugs. As results, we identified various genes that are responsible for the changes in the drug sensitivity. These results will be helpful to reduce the adverse effects of immunosuppressants, to identify novel target molecules for antifugal drugs, to overcome the drug resistance, or to find a new clinical application of valproic acid.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (25 results)

All 2013 2012 2011 2010 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (10 results) Remarks (4 results)

  • [Journal Article] Genetic screening for regulators of Prz1, a transcriptional factor acting downstream of calcineurin in fission yeast2012

    • Author(s)
      Atsushi Koike, Toshiaki Kato, Reiko Sugiura, Yan Ma, Yuki Tabata, Koji Ohmoto, Susie O. Sio, Takayoshi Kuno
    • Journal Title

      J. Biol. Chem.

      Volume: 287 Issue: 23 Pages: 19294-19303

    • DOI

      10.1074/jbc.m111.310615

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Genetic and Pharmacological Analysis of Isoprenoid Pathway by LC-MS/MS in Fission Yeast2012

    • Author(s)
      Tomonori Takami
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 11 Pages: e49004-e49004

    • DOI

      10.1371/journal.pone.0049004

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studies on the Roles of Clathrin-Mediated Membrane Trafficking and Zinc Transporter Cis4 in the Transport of GPI-Anchored Proteins in Fission Yeast2012

    • Author(s)
      Jaiseng W, Fang Y, Ma Y, Sugiura R, Kuno T
    • Journal Title

      PLoS ONE

      Volume: 7(7) Issue: 7 Pages: 19294-19303

    • DOI

      10.1371/journal.pone.0041946

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast2012

    • Author(s)
      Ryuko S, Ma Y, Ma N, Sakaue M, Kuno T
    • Journal Title

      Mol Genet Genomics

      Volume: 287(8):651-62 Issue: 8 Pages: 651-62

    • DOI

      10.1007/s00438-012-0705-9

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A measurable activation of the bZIP transcription factor Atf1 in a fission yeast strain devoid of stress-activated and cell-integrity MAPK activities2012

    • Author(s)
      Xin Zhou
    • Journal Title

      J. Biol. Chem.

      Volume: 287 Pages: 23434-23439

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A genome-wide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis2012

    • Author(s)
      Yue Fang
    • Journal Title

      Antimicrob. Agents Chemother.

      Volume: 56 Pages: 1949-1959

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A genome-wide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis2012

    • Author(s)
      房月(Yue Fang)
    • Journal Title

      Antimicrobial Agents and Chemotherapy

      Volume: (in press)(掲載確定)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-Wide Screening for Genes Associated with FK506 Sensitivity in Fission Yeast2011

    • Author(s)
      Yan Ma, Weijuan Jiang, Qingbin Liu, Sayomi Ryuko, Takayoshi Kuno
    • Journal Title

      PLoS ONE

      Volume: 6(8) Issue: 8 Pages: e23422-e23422

    • DOI

      10.1371/journal.pone.0023422

    • NAID

      120005468181

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transient Receptor Potential (TRP) and Cch1-Yam8 Channels Play Key Roles in the Regulation of Cytoplasmic Ca2+ in Fission Yeast2011

    • Author(s)
      馬艶(Yan Ma)
    • Journal Title

      PLoS ONE

      Volume: 6(7) Issue: 7 Pages: e22421-e22421

    • DOI

      10.1371/journal.pone.0022421

    • NAID

      120005468188

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of a fission yeast mutant that is sensitive to valproic acid and defective in the gene encoding Ric1, a putative component of Ypt/Rab-specific GEF for Ryh1 GTPase.2010

    • Author(s)
      馬艶(Yan Ma), et al.
    • Journal Title

      Mol.Genet.Genomics

      Volume: 284 Pages: 161-171

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MAPKKK-dependent and -independent activation of Styl stress MAPK in fission yeast2010

    • Author(s)
      周きん(Xin Zhou), et al.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 32818-32823

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ラパマイシン標的分子Tor2制御におけるNpr2の役割:ゲノムワイド解析による解明2013

    • Author(s)
      馬 艶
    • Organizer
      第86回 日本薬理学会年会  3月21日~3月23日(福岡), 2013
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 分裂酵母におけるNO応答転写調節メカニズムの解明2012

    • Author(s)
      烏干格日楽
    • Organizer
      第122回 日本薬理学会近畿部会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] 分裂酵母におけるDNA 損傷応答メカニズムの解明2012

    • Author(s)
      山田優希
    • Organizer
      第122回 日本薬理学会近畿部会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] 低グルコースで活性化される転写因子Rst2の転写活性のリアルタイムモニタリング2012

    • Author(s)
      加藤俊明
    • Organizer
      第122回 日本薬理学会近畿部会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] 分裂酵母ノックアウトライブラリーを用いた亜鉛感受性及び耐性遺伝子のゲノムワイドスクリーニング2012

    • Author(s)
      長谷川よしの
    • Organizer
      第122回 日本薬理学会近畿部会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] A genome-wide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.2011

    • Author(s)
      Yue Fang
    • Organizer
      British Pharmacology Society Meeting
    • Place of Presentation
      London, Great Britain
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] A genome-wide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis2011

    • Author(s)
      房月(Yue Fang)
    • Organizer
      BPS Winter Meeting, London. 2011
    • Place of Presentation
      London (Queen Elizabeth II Conference Center) U.K.
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Transient Receptor Potential (TRP) and Cch1-Yam8 Channels Play Key Roles in the Regulation of Cytoplasmic Ca^<2+> in Fission Yeast2011

    • Author(s)
      馬艶(Yan Ma)
    • Organizer
      Yeast Cell Biology, 2011
    • Place of Presentation
      New York (Cold Spring Harbor) U.S.A.
    • Year and Date
      2011-08-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Two Phosphoinositide-dependent Kinase-1 (PDK1) Homologues, Ksg1 and Ppk21, differently functions to downstream effector pathways in Fission Yeast2011

    • Author(s)
      張麗麗(Lili Zhang)
    • Organizer
      Yeast Cell Biology, 2011
    • Place of Presentation
      New York (Cold Spring Harbor) U.S.A.
    • Year and Date
      2011-08-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Transient Receptor Potential (TRP) and Cch1-Yam8 Channels Play Key Roles in the Regulation of Cytoplasmic Ca2+ in Fission Yeast2011

    • Author(s)
      Yan Ma
    • Organizer
      Cold Spring Harbor Meeting on Yeast Cell Biology
    • Place of Presentation
      Cold Spring Harbor, New York, USA
    • Year and Date
      2011-08-16
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.med.kobe-u.ac.jp/pharma/welcome.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 研究の内容と業績

    • URL

      http://www.med.kobe-u.ac.jp/pharma/histor.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.kobe-u.ac.jp/pharma/welcome.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.kobe-u.ac.jp/pharma/histor.html#publications

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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