Roles of chondroitin sulfate proteoglycan in maintenance of tissue structure and its remodeling under pathological conditions

• Project/Area Number: 22390077
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Aichi Medical University
• Principal Investigator: 渡辺 秀人 愛知医科大学, 分子医科学研究所, 教授 (90240514)
• Co-Investigator(Renkei-kenkyūsha): 杉浦 信夫 愛知医科大学, 分子医科学研究所, 准教授 (90454420) ; 永井 尚子 愛知医科大学, 分子医科学研究所, 助教 (00367799) ; 幡野 その子 愛知医科大学, 分子医科学研究所, 助教 (40434625)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: プロテオグリカン / 細胞外マトリックス / コンディショナルノックアウトマウス / バーシカン / 創傷治癒 / ノックアウトマウス / グリコサミノグリカン / 微小環境 / コンドロイチン硫酸 / ノックアウトマウ

Research Abstract

In this project, we are attempting to understand in vivo roles of versican (Vcan), a chondroitin sulfate proteoglycan in the extracellular matrix, in maintenance of tissue structure and several pathological conditions. By analyzing Vcan conditional knockout mice with adenoviral cre expression systems, we found that Vcan facilitatesgranulation during wound healing and suppress growth of transplant tumor.

Research Products

• [Journal Article] Construction of a chondroitin sulfate library with defined structures and analysis of molecular interactions. (2012)
  • Author(s): Sugiura N, Shioiri T, Chiba M, Sato T, Narimatsu H, Kimata K, Watanabe H
  • Journal Title: J Biol Chem Volume: 287 Issue: 52 Pages: 43390-43400
  • DOI: 10.1074/jbc.m112.412676
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate synthase-2 is necessary for chain extension of chondroitin sulfate but not critical for skeletal development. (2012)
  • Author(s): Ogawa H, Hatano S, Sugiura N, Nagai N, Sato T, Shimizu K, Narimatsu H, Kimata K, Watanabe H
  • Journal Title: PLoS ONE Volume: 7 Issue: 8 Pages: 1-12
  • DOI: 10.1371/journal.pone.0043806
  • Peer Reviewed
• [Journal Article] Versican/PG-M is essential for ventricular septal formation subsequent to cardiac atrioventricular cushion development (2012)
  • Author(s): S. Hatano, K. Kimata, N. Hiraiwa, M. Kusakabe, Z. Isogai, E. Adachi, T. Shinomura, and H. Watanabe
  • Journal Title: Glycobiology Volume: 22 (9) Issue: 9 Pages: 1268-1277
  • DOI: 10.1093/glycob/cws095
  • Peer Reviewed
• [Journal Article] YAG laser treatment causes rapid degeneration and regeneration of collagen fibers in pig skin and facilitates fibroblast growth. (2012)
  • Author(s): Kono A, Oguri A, Yokoo K, Watanabe H
  • Journal Title: J Plast Surg Hand Surg Volume: 46 Issue: 5 Pages: 308-312
  • DOI: 10.3109/2000656x.2012.696197
• [Journal Article] Keratan Sulfate Restricts Neural Plasticity after Spinal Cord Injury (2011)
  • Author(s): Shiro Imagama, Yukihiro Matsuyama(14番目)
  • Journal Title: J Neurosci Volume: 31 Issue: 47 Pages: 17091-17102
  • DOI: 10.1523/jneurosci.5120-10.2011
  • NAID: 120005946849
  • Peer Reviewed
• [Journal Article] Cell surface heparin sulfate chains regulate local reception of FGF signaling in the mouse embryo (2011)
  • Author(s): 下川佳世
  • Journal Title: Developmental Cell Volume: 21 Issue: 2 Pages: 257-272
  • DOI: 10.1016/j.devcel.2011.06.027
  • Peer Reviewed
• [Journal Article] Baculovirus envelope protein ODV-66 is a novel chondroitinase with distinct substrate specificity. (2011)
  • Author(s): Sugiura N, Setoyama Y, Chiba M, Kimata K, Watanabe H
  • Journal Title: J Biol Chem Volume: 286 Issue: 33 Pages: 29026-29034
  • DOI: 10.1074/jbc.m111.251157
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondralossification and aggrecan metabolism. (2011)
  • Author(s): Sato T, Kudo T, Ikehara Y, Ogawa H, Hirano T, Kiyohara K, Hagiwara K, Togayachi A, Ema M, Takahashi S, Kimata K, Watanabe H, Narimatsu H
  • Journal Title: J Biol Chem Volume: 286 Issue: 7 Pages: 5803-5812
  • DOI: 10.1074/jbc.m110.159244
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate synthase-2/chondroitin polymerizing factor has two variants with distinct function. (2010)
  • Author(s): Ogawa H, Shionyu M, Sugiura N, Hatano S, Nagai N, Kubota Y, Nishiwaki K, Sato T, Gotoh M, Narimatsu H, Shimizu K, Kimata K, Watanabe H
  • Journal Title: J Biol Chem Volume: 285 Issue: 44 Pages: 34155-34167
  • DOI: 10.1074/jbc.m110.109553
• [Journal Article] Deficiency in the serum-derived hyaluronan-associated protein-hyaluronan complex enhances airway hyperresponsiveness in a murine model of asthma. (2010)
  • Author(s): Zhu L. Zhuo L, Kimata K, Yamaguchi E, Watanabe H, Aronica MA, Hascall, VC, Baba K
  • Journal Title: Int Arch Allergy Immunol Volume: 153 Issue: 3 Pages: 223-233
  • DOI: 10.1159/000314362
• [Journal Article] Versican facilitates chondrocyte differentiation and regulates joint morphogenesis. (2010)
  • Author(s): Choocheep K, Hatano S, Takagi H, Watanabe H, Kimata K, Kongtawelert P, Watanabe H
  • Journal Title: J Biol Chem Volume: 285 Issue: 27 Pages: 21114-21125
  • DOI: 10.1074/jbc.m109.096479
• [Journal Article] The Glucuronyl transferase activity of KfiC from Escherichia coli strain K5 requires association of KfiA ; the essential enzymes for production of K5 polysaccharide, N-acetylheparosan (2010)
  • Author(s): Sugiura N, et al
  • Journal Title: J Biol Chem Volume: 285 Pages: 1597-1606
  • Peer Reviewed
• [Journal Article] Versican facilitates chondrocyte differentiation and regulates joint morphogenesis (2010)
  • Author(s): Choocheep K, eta al
  • Journal Title: J Biol Chem Volume: 285 Pages: 21114-21125
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate synthase-2/chondroitin polymerizing factor has two variants with distinct function (2010)
  • Author(s): Ogawa, H, et al
  • Journal Title: J Biol Chem Volume: 285 Pages: 34155-34167
  • Peer Reviewed
• [Journal Article] Involvement of heparan sulfate6-O-sulfation in regulation of energy metabolism and alteration of thyroid hormone levels in male mice
  • Author(s): Nagai N, Habuchi H, Sugaya N, NakamuraM, Imamura T, Watanabe H, Kimata K
  • Journal Title: Glycobiology Volume: (in press)
• [Presentation] Chondroitin Sulfate synthase-2/chondroitin polymerizing factor (2012)
  • Author(s): Hideto Watanabe, Hiroyasu Ogawa, Masafumi Shionyu, Nobui Sugiura, Sonoko Hatano, Naoko Nagai, Takashi Sato Hisashi Narimatsu, Katsuji Shimizu, Koji Kimata
  • Organizer: characterization and impact on chondroitin sulfate biosynthesis 2012 Joint Meeting of the Society for Glycobiology & American Society for
  • Place of Presentation: San Diego,U.S.A.
• [Presentation] 組換え硫酸基転移酵素を用いた人工コンドロイチン硫酸の合成と構造解析 (2012)
  • Author(s): 塩入達政、渡辺秀人、杉浦信夫
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島
• [Presentation] 各種バキュロウイルスのコンドロイチナーゼ活性とカイコ囲食膜のコンドロイチン硫酸 (2012)
  • Author(s): 杉浦信夫、吉村真弓、池田素子、小林迪弘、渡辺秀人
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島
• [Presentation] YAG laser treatment causes rapid degeneration and regeneration of collagen fibers in pig skin and facilitates fibroblast growth (2012)
  • Author(s): Ayuko Kono, Akiko Oguri, KazuhisaYokoo, Hideto Watanabe
  • Organizer: 23rd FECTS and ISMB Joint Meeting(Katowice, Poland)
• [Presentation] 間葉系組織の構築と維持におけるバーシカンの役割 (2012)
  • Author(s): 渡辺秀人
  • Organizer: 第101回日本病理学会春期総会
  • Place of Presentation: 東京
• [Presentation] 間葉系組織の構築と維持におけるバーシカンの役割 (2012)
  • Author(s): 渡辺秀人
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] YAG laser treatment causes rapid degeneration and regeneration of collagen fibers in pig skin and facilitates fibroblast growth (2012)
  • Author(s): Watanabe H
  • Organizer: 23rd FECTS meeting
  • Place of Presentation: カトヴィッツェ ポーランド
• [Presentation] Chondroitin sulfate synthase-2/chondroitin polymerizing factor: characterization and impact on chondroitin sulfate biosynthesis. (2012)
  • Author(s): Watanabe H
  • Organizer: Society for Glycobiology, American Society for Matrix Biology Joint Meeting 2012
  • Place of Presentation: サンディエゴ
• [Presentation] 軟骨と関節の形成・維持におけるバーシカンの役割 (2011)
  • Author(s): 渡辺秀人
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2011-08-30
• [Presentation] バキュロウイルス由来の新奇なコンドロイチナーゼ (2011)
  • Author(s): 杉浦信夫、瀬戸山由佳、千葉美恵、木全弘治、渡辺秀人
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: 長岡
  • Year and Date: 2011-07-11
• [Presentation] コンドロイチン硫酸オリゴ糖および多糖体の酵素合成 (2011)
  • Author(s): 塩入達政、伊藤浩美、千葉美恵、瀬戸山由佳、堤内要、渡辺秀人、成松久、木全弘治、杉浦信夫
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: 長岡
  • Year and Date: 2011-07-11
• [Presentation] 軟骨分化と関節形成におけるバーシカンの役割 (2011)
  • Author(s): 渡辺秀人
  • Organizer: 日本軟骨代謝学会
  • Place of Presentation: 福岡
  • Year and Date: 2011-03-06
• [Presentation] 軟骨分化と関節形成におけるバーシカンの役割 (2011)
  • Author(s): 渡辺秀人
  • Organizer: 第24回日本軟骨代謝学会
  • Place of Presentation: 福岡
  • Year and Date: 2011-03-04
• [Presentation] Chondroitin sulfate synthase-2/chondroitin polymerizing factor: characterization and impact on chondroitin sulfate biosynthesis (2011)
  • Author(s): Hiroyasu Ogawa, Masashi Shionyu, Nobuo Sugiura, Sonoko Hatano, Naoko Nagai, Takashi Sato, Masanori Gotoh, Hisashi Narimatsu, Katsuji Shimizu, Koji Kimata, Hideto Watanabe
  • Organizer: 7th International Conference onProteoglycans
  • Place of Presentation: Sydney, Australia
• [Presentation] 高硫酸化コンドロイチン硫酸の酵素合成と低硫酸化単位を切断する新奇コンドロイチナーゼ (2011)
  • Author(s): 杉浦信夫
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: 長岡
• [Presentation] Versican facilitates chondrocyte differentiation and regulates joint morphogenesis (2011)
  • Author(s): Watanabe H
  • Organizer: 7th International Conference on Proteoglycans
  • Place of Presentation: シドニー
• [Presentation] Versican facilitates chondrocyte differentiation and regulates joint morphogenesis. (2010)
  • Author(s): Hideto Watanabe, Kanyamas Choocheep, Sonoko Hatano
  • Organizer: 2010 Biennial Meeting, The AmericanSociety for Matrix Biology
  • Place of Presentation: Charleston, U.S.A.
  • Year and Date: 2010-10-25
• [Presentation] グリコサミノグリカンの構造と生体内機能 (2010)
  • Author(s): 渡辺秀人
  • Organizer: 日本尿路結石学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-08-28
• [Presentation] Versican facilitates chondrocyte differentiation and regulates joint morphogenesis (2010)
  • Author(s): Watanabe H, et al
  • Organizer: 14^<th> Gordon Research Conference on Proteoglycans
  • Place of Presentation: アンドーバー(NH,USA)
  • Year and Date: 2010-07-14
• [Remarks]
  • URL: http://www.aichi-med-u.ac.jp/su10/su1009/index.html
• [Remarks] 愛知医科大学 分子医科学研究所
  • URL: http://www.aichi-med-u.ac.jp/su10/su1009/index.html