Mechanism of Listeria virulence factor-mediated regulation of innate immune response via swapping of virulence genes among Listeria species

• Project/Area Number: 22390082
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kyoto University
• Principal Investigator: MITSUYAMA Masao 京都大学, 医学研究科, 教授 (10117260)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 細菌 / リステリア / 病原因子 / 免疫応答 / 細胞内寄生菌 / サイトカイン / マクロファージ

Research Abstract

In the present study the molecular mechanism by which Listeria virulence factors regulate host innate immune response was investigated by swapping of corresponding virulence genes among Listeria species. Peritoneal exudate macrophages were infected with various Lm mutants, and cytokine production and the inflammasome activation was monitored. Results showed that AIM2 serves as an intracellular sensor of Lm to induce inflammasome and caspase-1 activation. ASC also plays an essential role incaspase-1 activation after entry of Lm into the cytoplasm. Furthermore, it was clearly shown that the Thr233 of LLO is an important residue to induce caspase-1 activation

Research Products

• [Journal Article] Del Carmen Sasiain M. Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models (2013)
  • Author(s): Yokobori N, Lopez B, Geffner L, Sabio Y Garcia C, Schierloh P, Barrera L, de la Barrera S, Sakai S, Kawamura I, Mitsuyama M, Ritacco V
  • Journal Title: Infect Genet Evol. Volume: 16 Pages: 151-156
  • DOI: 10.1016/j.meegid.2013.01.007
  • Peer Reviewed
• [Journal Article] Nitric oxide inhibits the NLRP3 inflammasome (2012)
  • Author(s): Hernandez-Cuellar E, Tsuchiya K, Hara H, Fang R, Sakai S, Kawamura I, Akira S, Mitsuyama M
  • Journal Title: J Immunol. Volume: 189 Issue: 11 Pages: 2012-2012
  • DOI: 10.4049/jimmunol.1202479
  • Peer Reviewed
• [Journal Article] Listeria monocytogenes strain-specific impairment of the TetR regulator underlies the drastic increase in cyclic di-AMP secretion and beta interferon-inducing ability (2012)
  • Author(s): Yamamoto T, Hara H, Tsuchiya K, Sakai S, Fang R, Matsuura M, Nomura T, Sato F, Mitsuyama M, Kawamura I
  • Journal Title: Infect Immun Volume: 80 Issue: 7 Pages: 2323-2332
  • DOI: 10.1128/iai.06162-11
  • Peer Reviewed
• [Journal Article] Evasion of human innate immunity without antagonizing TLR4 by mutant Salmonella enterica serovar Typhimurium having penta-acylated lipid A (2012)
  • Author(s): Notohiro Matsuura, Kiyoshi Kawasaki, Kazuyoshi Kawahara, Masao Mitsuyama
  • Journal Title: Innate Immunity Volume: 18(in press)(印刷中)
  • NAID: 120005398390
  • Peer Reviewed
• [Journal Article] HTLV-1 bZIP factor impairs cell-mediated immunity by suppressing production of Th1 cytokines. (2012)
  • Author(s): Sugata K, Satou Y, Yasunaga JI, Hara H, Ohshima K, Utsunomiya A, Mitsuyama M, Matsuoka M.
  • Journal Title: Blood Volume: 119 Issue: 2 Pages: 434-444
  • DOI: 10.1182/blood-2011-05-357459
  • NAID: 120003752111
  • Peer Reviewed
• [Journal Article] Critical roles of ASC in flammasomes in caspase-1 activation and host innate resistance to Streptococcus pneumoniae infection. (2011)
  • Author(s): R. Fang, K. Tsuchiya, I. Kawamura, Y. Shen, H. Hara, S. Sakai, T. Yamamoto, T. Fernandes-Alnemri, R. Yang, E. Hernandez-Cuellar, S. R. Dewamitta, Y. Xu, H. Qu, E. S. Alnemri, and M. Mitsuyama
  • Journal Title: J. Immunol Volume: 187 Issue: 9 Pages: 4890-4899
  • DOI: 10.4049/jimmunol.1100381
  • Peer Reviewed
• [Journal Article] Expression of the Mycobacterium tuberculosis PPE37 protein in Mycobacterium smegmatis induces lowtumour necrosis factor alpha and interleukin 6 production in murine macrophages. (2011)
  • Author(s): S. Daim, T. Nomura, I. Kawamura, H. Hara, K. Tsuchiya, T. Kurenuma, Y. Shen, T. Yamamoto, H. Qu, S. Sakai, Y. Xu, and M. Mitsuyama.
  • Journal Title: J. Med. Microbiol Volume: 60 Issue: 5 Pages: 582-591
  • DOI: 10.1099/jmm.0.026047-0
  • Peer Reviewed
• [Journal Article] AIM2はインフラマソームの活性化に関与する (2011)
  • Author(s): 土屋晃介、光山正雄
  • Journal Title: 生体の科学 Volume: 62 Pages: 202-207
• [Journal Article] リステリア (2011)
  • Author(s): 光山正雄
  • Journal Title: 感染と消毒 Volume: 18 Pages: 32-36
• [Journal Article] PD-1-PD-L1 pathway impairs Th1 immune response in the late stage of infection with Mycobacterium bovis bacillus Calmette-Guerin (2010)
  • Author(s): S. Sakai, I. Kawamura, T. Okazaki, K. Tsuchiya, R. Uchiyama, and M. Mitsuyama
  • Journal Title: Int. Immunol Volume: 22 Issue: 12 Pages: 915-925
  • DOI: 10.1093/intimm/dxq446
  • Peer Reviewed
• [Journal Article] Involvement of absent in Melanoma 2 in inflammasome activation in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): K. Tsuchiya, H. Hara, I. Kawamura, T. Nomura, T. Yamamoto, S. Daim, S. R. Dewamitta, Y. Shen, R. Fang, and M. Mitsuyama
  • Journal Title: J. Immunol Volume: 185 Issue: 2 Pages: 1186-1195
  • DOI: 10.4049/jimmunol.1001058
  • Peer Reviewed
• [Journal Article] Toll-like receptor 2-andMyD88-dependent phosphatidylinositol3-kinase and Rac1 activation facilitates the phagocytosis of Listeria monocytogenes by urine macrophages. (2010)
  • Author(s): Y. Xhen, I. Kawamura, T. Nomura, K. Tsuchiya, H. Hara, S. R. Dewamitta, S. Sakai, H. Qu, S. Daim, T. Yamamoto, and M. Mitsuyama
  • Journal Title: Infect. Immun Volume: 78 Issue: 6 Pages: 2857-2867
  • DOI: 10.1128/iai.01138-09
  • Peer Reviewed
• [Journal Article] Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): S.R. Dewamitta T. Nomura, I. Kawamura, H. Hara, K. Tsuchiya, T. Kurenuma, Y. Shen, S. Daim, T. Yamamoto, H. Qu, S. Sakai, Y. Xu, and M. Mitsuyama.
  • Journal Title: Infect. Immun. Volume: 78 Pages: 1884-1894
  • Peer Reviewed
• [Journal Article] α-galactosylceramide promotes killing of Listeria monocytogenes within the macrophage phagosome through invariant NKT-cell activation (2010)
  • Author(s): M. Emoto, T. Yoshida, T. Fukuda, I. Kawamura, M. Mitsuyama, E. Kita, R. Hurwitz, S. H. E. Kaufmann, and Y. Emoto
  • Journal Title: Infect. Immun Volume: 78 Issue: 6 Pages: 2667
  • DOI: 10.1128/iai.01441-09
  • Peer Reviewed
• [Journal Article] Involvement of absent in melanoma 2 in inflammasome activation in macrophages infected with Listeria monocytognes (2010)
  • Author(s): Tsuchiya Kohsuke, et al.
  • Journal Title: Journal of Immunology Volume: 185 Pages: 1186-1195
  • Peer Reviewed
• [Journal Article] Toll-like receptor 2-and Myd88-dependent phosphatidylinositol 3-kanase and Rac1 activation facilitates the phagocytosis of Listeiria monocytogenes by murine macrophages (2010)
  • Author(s): Shen Yanna, et al.
  • Journal Title: Infection and Immunity Volume: 78 Pages: 2857-2867
  • Peer Reviewed
• [Journal Article] Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): Dewamitta Ramiyamali, et al.
  • Journal Title: Infection and Immunity Volume: 78 Pages: 1884-1894
  • Peer Reviewed
• [Presentation] The Thr223 in listeriolysin O is essential for the ability to induce the inflammasome activation. (2013)
  • Author(s): Hara, H.
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] The role of listeriolysin O in calpain activation in Listeria monocytogenes-infected macrophages (2013)
  • Author(s): Qu, H.
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] Role for NLRP3 and ASC in Streptococcus pneumoniae infection (2013)
  • Author(s): Tsuchiya, K.
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] The role of listeriolysin O in calpain activation in Listeria monocytogenes-infected macrophages. (2013)
  • Author(s): Qu, H.
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] Role for NLRP3 and ASC in Streptococcus pneumoniae infection. (2013)
  • Author(s): Tsuchiya, K.
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] E. Nitric oxide-dependent suppression of the NLRP3 inflammasome activation. (2012)
  • Author(s): Hernandez-Cuellar
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-12-06
• [Presentation] Involvement of the domain 3 of listeriolysin O in inflammasome activation induced by Listeria monocytogenes infection (2012)
  • Author(s): Hara, H.
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-12-06
• [Presentation] The inflammasome adaptor ASC plays a host-detrimental role in lethal infection with Listeria monocytogenes. (2012)
  • Author(s): Tsuchiya, K
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-12-05
• [Presentation] E. Nitric oxide inhibits the NLRP3 inflammasome (2012)
  • Author(s): Hernandez-Cuellar
  • Organizer: 第65回日本細菌学会関西支部総会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-11-17
• [Presentation] Critical role of listeriolysin O in clpain activation, which is triggered after evasion of Listeria monocytogenes from the phagosome into the cytoplasm of infected macrophages (2012)
  • Author(s): Qu, H
  • Organizer: 第65回日本細菌学会関西支部総会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-11-17
• [Presentation] リステリア感染における主要病原因子 listeriolysin O 依存的なインフラマソーム応答の役割 (2012)
  • Author(s): 原 英樹
  • Organizer: 第23回日本生体防御学会学術総会
  • Place of Presentation: 東京都
  • Year and Date: 2012-07-11
• [Presentation] 結核菌による宿主感染防御の発現制御 (2012)
  • Author(s): 河村伊久雄
  • Organizer: 第87回日本結核病学会
  • Place of Presentation: 広島市
  • Year and Date: 2012-05-10
• [Presentation] PD-1 シグナル経路による抗結核防御免疫の制御. (2012)
  • Author(s): 河村伊久雄
  • Organizer: 第82回実験結核研究会
  • Place of Presentation: 広島市
  • Year and Date: 2012-05-09
• [Presentation] he role of inflammasomes in bacterial infections (2012)
  • Author(s): 土屋晃介、原英樹、方仁東、酒井俊佑、Eduardo Hernandez-Cuellar、河村伊久雄、光山正雄
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-29
• [Presentation] Nitric oxide-dependent suppression of the NLRP3 inflammasome activation (2012)
  • Author(s): Eduardo Hernandez-Cuellar、河村伊久雄、原英樹、土屋晃介、方仁東、山本武司、酒 井 俊 佑 、 光 山 正 雄
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-29
• [Presentation] PD-1 deficiency causes CD4 T cell-mediated exacerbation of Mycobacterium tuberculosis infection (2012)
  • Author(s): 酒井俊佑、河村伊久雄、土屋晃介、光山正雄
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-28
• [Presentation] Analysis of the region responsible for inflammasome activation in listeriolysin O (2012)
  • Author(s): 原英樹、Adaobi Igwilo、土屋晃介、山本武司、 Eduardo Hernandez-Cuellar 、河村伊久雄、光山正雄
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-27
• [Presentation] The RD1 locus in the Mycobacterium tuberculosis genome contributes to secretion of ILmacrophages through the induction of calcium influx. (2011)
  • Author(s): I. Kawamura, R. Yang, C. Xi, S. R. Dewamitta, M. Mitsuyama, et al
  • Organizer: The 46 US-Japan cooperative medical science program Tuberculosis-Leprosy research conference
  • Place of Presentation: Ohmiya
  • Year and Date: 2011-12-08
• [Presentation] 致死性リステリア感染モデルにおける ASC の役割の解析 (2011)
  • Author(s): 土屋晃介、原英樹、酒井俊佑、河村伊久雄、光山正雄
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 2011-11-28
• [Presentation] Involvement of listeriolysin 0-mediated inflammasome activation in bacterial virulence upon Listeria monocytogenesinfection. (2011)
  • Author(s): H. Hara, K. Tsuchiya, T. Yamamoto, I. Kawamura and M. Mitsuyama
  • Organizer: 第40回日本免疫学会総会、
  • Place of Presentation: 千葉市
  • Year and Date: 2011-11-28
• [Presentation] 結核菌感染マクロファージの IL- 産生における RD1 遺伝子領域の役割 (2011)
  • Author(s): I. Kawamura, C. Xi, S. Sakai, K. Tsuchiya, H. Hara and M. Mitsuyama
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 2011-11-28
• [Presentation] Listeria monocytogenes の菌株特異的なI 型 IFN 誘導能の違いは tetR の自然発生的な欠損に依存する (2011)
  • Author(s): IT. Yamamoto, H. Hara, K. Tsuchiya, S. Sakai, I. Kawamura and M. Mitsuyama.
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 2011-11-28
• [Presentation] 致死性リステリア感染モデルにおけるASCの役割の解析/A host-detrimental role of ASC in a lethal infection with Listeria monocytogenes (2011)
  • Author(s): 土屋晃介、原英樹、酒井俊祐、河村伊久雄、光山正雄
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉県千葉市)
  • Year and Date: 2011-11-28
• [Presentation] Involvement of listeriolysin O-mediated inflammasome activation in bacterial virulence upon Listeria monocytogenes infection (2011)
  • Author(s): Hideki Hara, Kohsuke Tsuchiya, Takeshi Yamamoto, Ikuo Kawamura, Masao Mitsuyama
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉県千葉市)
  • Year and Date: 2011-11-28
• [Presentation] 結核菌感染マクロファージのIL-1alpha産生におけるRD1遺伝子領域の役割/The RD1 locus in the Mycobacterium tuberculosis genome contributes to secretion of IL-1alpha from infected macrophages through the induction of calcium influx (2011)
  • Author(s): Ikuo Kawamura, Chen Xi, Shunsuke Sakai, Takeshi Yamamoto, Kohsuke Tsuchiya, Hideki Hara, Masao Mitsuyama
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉県千葉市)
  • Year and Date: 2011-11-28
• [Presentation] Listeria monocytogenesの菌株特異的なI型IFN誘導能の違いはtetRの自然発生的な欠損に依存する/A naturally occurring deletion in the tetR gene underlies drastic differences among virulent Listeria monocytogenes strains in the ability to induce type I IFNs and to secrete cyclic di-AMP (2011)
  • Author(s): Takeshi Yamamoto, Hideki Hara, Kohsuke Tsuchiya, Shunsuke Sakai, Ikuo Kawamura, Masao Mitsuyama
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉県千葉市)
  • Year and Date: 2011-11-28
• [Presentation] Expression of the Mycobacterium tuberculosis PPE37 protein in Mycobacterium smegmatis induces low tumor necrosis factor alpha and interleukin 6 production in murine macrophages (2011)
  • Author(s): S. Daim, I. Kawamura, K. Tsuchiya, H. Hara, T. Yamamoto, H. Qu and M. Mitsuyama.
  • Organizer: 第64回日本細菌学会関西支部総会
  • Place of Presentation: 堺市
  • Year and Date: 2011-11-19
• [Presentation] リステリア感染における主要病原因子listeriolysin Oのインフラマソーム形成を介した病原性への関与 (2011)
  • Author(s): 原英樹、土屋晃介、山本武司、河村伊久雄、光山正雄
  • Organizer: 第64回日本細菌学会関西支部総会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス学術交流会館(大阪府堺市)
  • Year and Date: 2011-11-19
• [Presentation] The RD1 locus in the Mycobacterium tuberculosis genome contributes to secretion of ILfrom infected macrophages through the induction of calcium influx (2011)
  • Author(s): I. Kawamura, K. Tsuchiya, H. Hara, R. Fang and M. Mitsuyama
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-08
• [Presentation] A host-detrimental role of ASC in a lethal infection with Listeria monocytogenes. (2011)
  • Author(s): K. Tsuchiya, H. Hara, R. Fang, S. Sakai, S. Daim, S. R. Dewamitta, C. Xi, H. Qu, I. Kawamura and M. Mitsuyama
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology.
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-08
• [Presentation] Participation of inflammasome activation mediated by Listeriolysin O in bacterial virulence upon Listeria monocytogenes infection. (2011)
  • Author(s): H. Hara, K. Tsuchiya, T. Yamamoto, I. Kawamura and M. Mitsuyama.
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-08
• [Presentation] Role of vorilence determinant of intracellular parasitic bacteria in cytokine response of the infected macrophages (2011)
  • Author(s): Masao Misuyama
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市)(招待講演)
  • Year and Date: 2011-09-08
• [Presentation] The RD1 locus in the Mycobacterium Tubeculosis genome contributes to secretion of IL-1α from infected macrophages through the induction of calcium influx (2011)
  • Author(s): Ikuo Kawamura, Kohsuke Tsuchiya, Hideki Hara, Rendong Fang, Masao Mitsuyama
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市)
  • Year and Date: 2011-09-08
• [Presentation] A host-detrimental role of ASC in a lethal infection with Listeria monocytogenes (2011)
  • Author(s): Kohsuke Tsuchiya, Hideki Hara, Rendong Fang, Shunsuke Sakai, Daim Sylvia, Sita R Dewamitta, Xi Chen, Huixin Qu, Ikuo Kawamura, Masao Mitsuyama
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市)
  • Year and Date: 2011-09-08
• [Presentation] Participation of inflammasome activation mediated by listeriolysin O in bacterial virulence upon Listeria monocytogenes infection (2011)
  • Author(s): Hideki Hara, Kohsuke Tsuchiya, Takeshi Yamamoto, Ikuo Kawamura, Masao Mitsuyama
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市)
  • Year and Date: 2011-09-08
• [Presentation] Critical roles of ASC inflammasomes in caspase-1 activation and host innate resistance to Streptococcus pneumoniae infection (2011)
  • Author(s): Rendong Fang, Kohsuke Tsuchiya, Ikuo Kawamura, Yanna Shen, Hideki Hara, Shunsuke Sakai, Takeshi Yamamoto, Ruili Yang, Edgar Eduardo Hernandez Cuellar, Sita R Dewamitta, Masao Mitsuyama
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市)
  • Year and Date: 2011-09-08
• [Presentation] PD-1 regulates the balance between protective and pathologic immune responses during murine tuberculosis (2011)
  • Author(s): S. Sakai, I. Kawamura, K. Tsuchiya, T. Okazaki and M. Mitsuyama.
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-07
• [Presentation] 結核菌感染マクロファージの interleukin-1 産生における RD-1 遺伝子領域の重要性 (2011)
  • Author(s): 河村伊久雄、酒井俊佑、土屋晃介、原英樹、光山正雄.
  • Organizer: 第22回日本生体防御学会総会
  • Place of Presentation: 那覇市
  • Year and Date: 2011-06-30
• [Presentation] 細胞内寄生性細菌の感染戦略と宿主の対応 (2010)
  • Author(s): 光山正雄
  • Organizer: 第42回日本小児感染症学会総会
  • Place of Presentation: 仙台国際センター(仙台市)(招待講演)
  • Year and Date: 2010-11-27
• [Presentation] 結核菌感染マクロファージのinterleukin- 産生における結核菌病原性関連遺伝子領域 RD1 の役割. (2010)
  • Author(s): 河村伊久雄, 楊瑞麗, 陳曦, S. R. Dewamitta, 酒井俊祐,土屋晃介,原英樹,光山正雄
  • Organizer: 第63回日本細菌学会関西支部総会
  • Place of Presentation: 枚方市
  • Year and Date: 2010-11-20
• [Presentation] Critical role of ASC in the activation of inflammasomes and resistance to pulmonary infection with Streptococcus pneumonia. (2010)
  • Author(s): R. Fang, K. Tsuchiya, I. Kawamura, Y. Shen, H. hara, and M. Mitsuyama.
  • Organizer: 第63回日本細菌学会関西支部総会
  • Place of Presentation: 枚方市
  • Year and Date: 2010-11-20
• [Presentation] Critical role of ASC in the activation of inflammasomes and resistance to pulmonary infection with Streptococcus pneumoniae (2010)
  • Author(s): R.Fang, K.Tsuchiya, I.Kawamura, Y.Shen, H.Hara, et.al.
  • Organizer: 第63回日本細菌学会関西支部総会
  • Place of Presentation: 関西医科大学付属病院講堂(大阪府)
  • Year and Date: 2010-11-20
• [Presentation] Listeriolysin O-dependent bacterial entry into cytoplasm is required for calpain activation and ILinfected with Listeria monocytogenes (2010)
  • Author(s): I. Kawamura, S. R. Dewamitta, T. Nomura, H. Hara, K. Tsuchiya, M. Mitsuyama, et al.
  • Organizer: The 14 International congress of Immunology
  • Place of Presentation: Osaka
  • Year and Date: 2010-08-25
• [Presentation] S. Sakai, I. Kawamura, K. Tsuchiya, T. Okazaki, and M. Mitsuyama (2010)
  • Author(s): S. Sakai, I. Kawamura, K. Tsuchiya, T. Okazaki, and M. Mitsuyama
  • Organizer: The 14thInternational Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] Involvement of type I interferon in inflammasome formation in macrophages infected with Listeria monocytogenes. (2010)
  • Author(s): H. Hara, K. Tsuchiya, I. Kawamura, T. Yamamoto, T. Nomura and M. Mitsuyama.
  • Organizer: The 14thInternational Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] The importance of Lmo2588/2589 in the ability of Listeria monocytogenes to induce IFN-beta production. (2010)
  • Author(s): T. Yamamoto, H. Hara, K. Tsuchiya, S. Sakai, T. Nomura, I. Kawamura and M. Mitsuyama.
  • Organizer: The 14thInternational Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] Involvement of type I interferon in inflammasome formation in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): H.Hara, K.Tsuchiya, I.Kawamura, T.Yamamoto, et.al.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] The importance of Lmo2588/2589 in the ability of Listeria monocytogenes to induce IFN-beta production (2010)
  • Author(s): T.Yamamoto, H.Hara, K.Tsuchiya, S.Sakai, T.Nomura, et.al
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸国際展示揚(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] Listeriolysin O-dependent bacterial entry into cytoplasm is required for calpain activation and IL-1α secretion in macrophages infected with Listeria monocytogenes (2010)
  • Author(s): I.Kawamura, S.D.Dewamitta, T.Nomura, H.Hara, K.Tsuchiya, Y.Shen, S.Daim, et.al.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] Listeriolysin O, a key factor of Listeria monocytogenes in the induction of immune response of the infected host. (2010)
  • Author(s): Masao Mitsuyama
  • Organizer: 9^<th> International Veterinary Immunology Symposium
  • Place of Presentation: タワーホール船堀(東京都)(招待講演)
  • Year and Date: 2010-08-17
• [Presentation] Listeria monocytogenes 感染後の産生誘導における lmo2589 遺伝子の重要性 (2010)
  • Author(s): 山本武司、原英樹、土屋晃介、酒井俊佑、野村卓正、S. Daim、河村伊久雄、光山正雄
  • Organizer: 第21回日本生体防御学会総会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-07-22
• [Presentation] リステリア感染マクロファージにおけるインフラマソーム活性化機序 (2010)
  • Author(s): 土屋晃介、原英樹、河村伊久雄、山本武司、S. Daim、S.R. Dewamitta、申艶那、方仁東、光山正雄
  • Organizer: 第21回日本生体防御学会総会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-07-22
• [Presentation] Listeria monocytogenes感染後のIFN-β産生誘導におけるLmo2589遺伝子の重要性 (2010)
  • Author(s): 山本武司、原英樹、土屋晃介、酒井俊介、野村卓正、Sylvia Daim, ら
  • Organizer: 第21回日本生体防御学会学術総会
  • Place of Presentation: 仙台市戦災復興記念館(宮城県)
  • Year and Date: 2010-07-22
• [Presentation] リステリア感染マクロファージにおけるインフラマソーム (2010)
  • Author(s): 土屋晃介、原英樹、河村伊久雄, ら
  • Organizer: 第21回日本生体防御学会学術総会
  • Place of Presentation: 仙台市戦災復興記念館(宮城県)
  • Year and Date: 2010-07-22
• [Presentation] PD-1 inhibitory signal is required for an orchestrated protective immunity to Mycobacterium tuberculosis in lung (2010)
  • Author(s): S. Sakai, I. Kawamura and M. Mitsuyama.
  • Organizer: The 45 U.S.-Japan cooperative medical science program Tuberculosis-Leprosy research conference
  • Place of Presentation: Boston, USA
  • Year and Date: 2010-07-13
• [Presentation] 細胞内寄生細菌リステリアの病原因子LLOによるサイトカイン誘導機構 (2010)
  • Author(s): 光山正雄
  • Organizer: 第16回日本ヘリコバクター学会学術総会
  • Place of Presentation: 芝蘭会館(京都市)(招待講演)
  • Year and Date: 2010-06-24
• [Presentation] PD-1シグナル経路による抗結核防御免疫の制御
  • Author(s): 河村伊久雄
  • Organizer: 第82回実験結核研究会
  • Place of Presentation: 広島市
• [Presentation] リステリア感染における主要病原因子listeriolysin O依存的なインフラマソーム応答の役割
  • Author(s): 原 英樹
  • Organizer: 第23回日本生体防御学会学術総会
  • Place of Presentation: 東京都
• [Presentation] Nitric oxide inhibits the NLRP3 inflammasome
  • Author(s): Hernandez-Cuellar, E.
  • Organizer: 第65回日本細菌学会関西支部総会
  • Place of Presentation: 神戸市
• [Presentation] Critical role of listeriolysin O in clpain activation, which is triggered after evasion of Listeria monocytogenes from the phagosome into the cytoplasm of infected macrophages.
  • Author(s): Qu, H.
  • Organizer: 第65回日本細菌学会関西支部総会
  • Place of Presentation: 神戸市
• [Presentation] Nitric oxide-dependent suppression of the NLRP3 inflammasome activation.
  • Author(s): Hernandez-Cuellar, E.
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸市
• [Presentation] Involvement of the domain 3 of listeriolysin O in inflammasome activation induced by Listeria monocytogenes infection.
  • Author(s): Hara, H.
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸市
• [Presentation] The inflammasome adaptor ASC plays a host-detrimental role in lethal infection with Listeria monocytogenes.
  • Author(s): Tsuchiya, K.
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸市
• [Book] 臨床と微生物、結核菌感染の病態 (2012)
  • Author(s): 酒井俊祐、光山正雄
  • Publisher: 近代出版
• [Book] 臨床と微生物 (2012)
  • Author(s): 酒井俊祐
  • Publisher: 近代出版
• [Book] 結核菌・抗酸菌感染防御機構 (2011)
  • Author(s): 河村伊久雄
  • Publisher: 日本臨床社
• [Book] 疫・感染生物学(分担執筆) (2011)
  • Author(s): 光山正雄
  • Total Pages: 204
  • Publisher: 岩波書店
• [Book] 病原菌の今日的意味(分担執筆) (2011)
  • Author(s): 光山正雄
  • Total Pages: 799
  • Publisher: 医薬ジャーナル社
• [Book] 現代生物科学入門 5.免疫・感染生物学(分担:細菌と感染) (2011)
  • Author(s): 光山正雄
  • Total Pages: 240
  • Publisher: 岩波書店
• [Book] 結核に対する感染防御機構 (2010)
  • Author(s): 河村伊久雄
  • Publisher: 日本結核病学会