Mechanism of Listeria virulence factor-mediated regulation of innate immune response via swapping of virulence genes among Listeria species
Project/Area Number |
22390082
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Bacteriology (including Mycology)
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | 細菌 / リステリア / 病原因子 / 免疫応答 / 細胞内寄生菌 / サイトカイン / マクロファージ |
Research Abstract |
In the present study the molecular mechanism by which Listeria virulence factors regulate host innate immune response was investigated by swapping of corresponding virulence genes among Listeria species. Peritoneal exudate macrophages were infected with various Lm mutants, and cytokine production and the inflammasome activation was monitored. Results showed that AIM2 serves as an intracellular sensor of Lm to induce inflammasome and caspase-1 activation. ASC also plays an essential role incaspase-1 activation after entry of Lm into the cytoplasm. Furthermore, it was clearly shown that the Thr233 of LLO is an important residue to induce caspase-1 activation
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Report
(4 results)
Research Products
(87 results)
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[Journal Article] Del Carmen Sasiain M. Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models2013
Author(s)
Yokobori N, Lopez B, Geffner L, Sabio Y Garcia C, Schierloh P, Barrera L, de la Barrera S, Sakai S, Kawamura I, Mitsuyama M, Ritacco V
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Journal Title
Infect Genet Evol.
Volume: 16
Pages: 151-156
DOI
Related Report
Peer Reviewed
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[Journal Article] Critical roles of ASC in flammasomes in caspase-1 activation and host innate resistance to Streptococcus pneumoniae infection.2011
Author(s)
R. Fang, K. Tsuchiya, I. Kawamura, Y. Shen, H. Hara, S. Sakai, T. Yamamoto, T. Fernandes-Alnemri, R. Yang, E. Hernandez-Cuellar, S. R. Dewamitta, Y. Xu, H. Qu, E. S. Alnemri, and M. Mitsuyama
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Journal Title
J. Immunol
Volume: 187
Issue: 9
Pages: 4890-4899
DOI
Related Report
Peer Reviewed
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[Journal Article] Expression of the Mycobacterium tuberculosis PPE37 protein in Mycobacterium smegmatis induces lowtumour necrosis factor alpha and interleukin 6 production in murine macrophages.2011
Author(s)
S. Daim, T. Nomura, I. Kawamura, H. Hara, K. Tsuchiya, T. Kurenuma, Y. Shen, T. Yamamoto, H. Qu, S. Sakai, Y. Xu, and M. Mitsuyama.
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Journal Title
J. Med. Microbiol
Volume: 60
Issue: 5
Pages: 582-591
DOI
Related Report
Peer Reviewed
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[Journal Article] Toll-like receptor 2-andMyD88-dependent phosphatidylinositol3-kinase and Rac1 activation facilitates the phagocytosis of Listeria monocytogenes by urine macrophages.2010
Author(s)
Y. Xhen, I. Kawamura, T. Nomura, K. Tsuchiya, H. Hara, S. R. Dewamitta, S. Sakai, H. Qu, S. Daim, T. Yamamoto, and M. Mitsuyama
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Journal Title
Infect. Immun
Volume: 78
Issue: 6
Pages: 2857-2867
DOI
Related Report
Peer Reviewed
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[Journal Article] Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes.2010
Author(s)
S.R. Dewamitta T. Nomura, I. Kawamura, H. Hara, K. Tsuchiya, T. Kurenuma, Y. Shen, S. Daim, T. Yamamoto, H. Qu, S. Sakai, Y. Xu, and M. Mitsuyama.
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Journal Title
Infect. Immun.
Volume: 78
Pages: 1884-1894
Related Report
Peer Reviewed
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[Presentation] Critical roles of ASC inflammasomes in caspase-1 activation and host innate resistance to Streptococcus pneumoniae infection2011
Author(s)
Rendong Fang, Kohsuke Tsuchiya, Ikuo Kawamura, Yanna Shen, Hideki Hara, Shunsuke Sakai, Takeshi Yamamoto, Ruili Yang, Edgar Eduardo Hernandez Cuellar, Sita R Dewamitta, Masao Mitsuyama
Organizer
IUMS2011
Place of Presentation
札幌コンベンションセンター(北海道札幌市)
Year and Date
2011-09-08
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