Regulation of virulence expression by retron including reverse transcriptase gene and roles as mobile genetic elements

• Project/Area Number: 22390083
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Hiroshima University
• Principal Investigator: SHIMAMOTO Tadashi 広島大学, 大学院・生物圏科学研究科, 教授 (90187443)
• Co-Investigator(Kenkyū-buntansha): SHIMAMOTO Toshi 広島大学, 大学院・生物圏科学研究科, 助教 (70583136)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 病原性 / 遺伝子発現制御 / 逆転写酵素,ゲノム / Vibrio cholerae / レトロン / msDNA / 逆転写酵素 / Vibrio / ゲノム / Vibrio choleare

Research Abstract

The orf540gene present downstream of the reverse transcriptase gene (ret) in retron-Vc95 was found to be related to pathogenicity in Vibrio choleraeO139. New retrons were isolated and identified from a clinical isolate of V. choleraenon-O1, non-O139 and an environmental isolate of V. mimicus. Plasmid-derived retron of a clinical isolate of Escherichia coliand new retron of Yersinia frederikseniiwere also analysed.

Research Products

• [Journal Article] A novel retron of Vibrio parahaemolyticusis closely related to retron-Vc95 of Vibrio cholerae. (2013)
  • Author(s): Toshi Shimamoto, Ashraf M. Ahmed, Tadashi Shimamoto
  • Journal Title: Journal of Microbiology
  • Peer Reviewed
• [Journal Article] A novel retron of Vibrio parahaemolyticus is closely related to retron-Vc95 of Vibrio cholerae (2013)
  • Author(s): Toshi Shimamoto
  • Journal Title: Journal of Microbiology Volume: 51 Issue: 3 Pages: 323-328
  • DOI: 10.1007/s12275-013-2715-7
  • Peer Reviewed
• [Journal Article] Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt. (2012)
  • Author(s): Ahmed M. Hammad, Tadashi Shimamoto
  • Journal Title: Microbiology and Immunology Volume: 56 Pages: 254-261
  • NAID: 40019239470
  • Peer Reviewed
• [Journal Article] Occurrence and characteristics of methicillin-resistant and -susceptible Staphylococcus aureusand methicillin-resistant coagulase-negative staphylococci from Japanese retail ready-to-eat raw fish. (2012)
  • Author(s): Ahmed M. Hammad, Wataru Watanabe, Tomoko Fujii, Tadashi Shimamoto
  • Journal Title: Journal of Food Microbiology Volume: 156 Pages: 286-289
  • Peer Reviewed
• [Journal Article] Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt (2012)
  • Author(s): Ashraf M. Ahmed
  • Journal Title: Microbiology and Immunology Volume: 56 Issue: 4 Pages: 254-261
  • DOI: 10.1111/j.1348-0421.2012.00429.x
  • Peer Reviewed
• [Journal Article] Occurrence and characteristics of methicillin-resistant and -susceptible Staphylococeus aureus and methicillin-resistant coagulase-negative staphylococci from Japanese retail ready-to-eat raw fish (2012)
  • Author(s): Ahmed M.Hammad
  • Journal Title: International Journal of Food Microbiology Volume: (in press) Issue: 3 Pages: 286-289
  • DOI: 10.1016/j.ijfoodmicro.2012.03.022
  • Peer Reviewed
• [Journal Article] Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt (2012)
  • Author(s): Ashraf M.Ahmed
  • Journal Title: Microbiology and Immunology Volume: (in press) Pages: no-no
  • DOI: 10.1111/j.1348-0421.2011.00429.x
  • Peer Reviewed
• [Journal Article] 抗菌薬のMICと生菌整腸薬の適応は? (2011)
  • Author(s): 島本 整,島本 敏
  • Journal Title: 薬局 Volume: 62 Pages: 423-427
• [Journal Article] Molecular characterization of antimicrobial resistance in Gram-negative bacteria isolated from bovine mastitis in Egypt (2011)
  • Author(s): Ashraf M. Ahmed, Tadashi Shimamoto
  • Journal Title: Microbiology and Immunology Volume: 55 Pages: 318-327
  • NAID: 10031121473
  • Peer Reviewed
• [Journal Article] Two novel retron elements are replaced with retron-Vc95 in Vibrio cholerae. (2011)
  • Author(s): Kumiko Inouye, Saori Tanimoto, Maki Kamimoto, Toshi Shimamoto, Tadashi Shimamoto
  • Journal Title: Microbiology and Immunology Volume: 55 Pages: 310-313
  • NAID: 10031121497
  • Peer Reviewed
• [Journal Article] Asymptomatic intramammary infection with multidrug-resistant Gram-negative bacteria in a research dairy farm: incidence and genetic basis of resistance. (2011)
  • Author(s): Ahmed M. Hammad, Tadashi Shimamoto
  • Journal Title: Journal of Veterinary Medical Science Volume: 73 Pages: 1089-1092
  • NAID: 130000677052
  • Peer Reviewed
• [Journal Article] Comparative study of different msDNA (multicopy single-stranded DNA) structures and phylogenetic comparison of reverse transcriptases (RTs): evidence for vertical inheritance. (2011)
  • Author(s): Rasel Das, Tadashi Shimamoto, Sultan Mohammad Zahid Hosen, Mohammad Arifuzzaman
  • Journal Title: Bioinformation Volume: 7 Pages: 174-179
  • Peer Reviewed
• [Journal Article] A novel msDNA (multi-copy single-stranded DNA) strain present in Yersinia frederiksenii ATCC 33641 contig01029 enteropathogenic bacteria with the genomic analysis of its retron. (2011)
  • Author(s): Rasel Das, Tadashi Shimamoto, Mohammad Arifuzzaman
  • Journal Title: Journal of Pathogens Volume: 2011
  • Peer Reviewed
• [Journal Article] Asymptomatic intramammary infection with multidrug-resistant Gram-negative bacteria in a research dairy farm : incidence and genetic basis of resistance (2011)
  • Author(s): Ahmed M.Hammad
  • Journal Title: Journal of Veterinary Medical Science Volume: 73 Pages: 1089-1092
  • Peer Reviewed
• [Journal Article] Comparative study of different msDNA (multicopy single-stranded DNA) structures and phylogenetic comparison of reverse transcriptases (RTs) : evidence for vertical inheritance (2011)
  • Author(s): Rasel Das
  • Journal Title: Bioinformation Volume: 7 Pages: 174-179
  • Peer Reviewed
• [Journal Article] A novel msDNA (multi-copy single-stranded DNA) strain present in Yersinia frederiksenii ATCC 33641 contig01029 enteropathogenic bacteria with the genomic analysis of its retron (2011)
  • Author(s): Rasel Das
  • Journal Title: Journal of Pathogens Volume: 2011 Pages: 1-6
  • DOI: 10.4061/2011/693769
  • Peer Reviewed
• [Journal Article] Two novel retron elements are replaced with retron-Vc95 in Vibrio cholerae (2011)
  • Author(s): Kumiko Inouye
  • Journal Title: Microbiology and Immunology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Molecular characterization of antimicrobial resistance in Gram-negative bacteria isolated from bovine mastitis in Egypt (2011)
  • Author(s): Ashraf M.Ahmed
  • Journal Title: Microbiology and Immunology Volume: (in press)
  • Peer Reviewed
• [Journal Article] 農業領域で使用される抗菌薬と耐性菌汚染. (2010)
  • Author(s): 島本 整
  • Journal Title: 臨床と微生物 Volume: 37 Pages: 679-683
• [Journal Article] 農業領域で使用される抗菌薬と耐性菌汚染 (2010)
  • Author(s): 島本整
  • Journal Title: 臨床と微生物 Volume: 37 Pages: 679-683
• [Presentation] Complete sequence of a novel multidrug resistance plasmid isolated from a clinical strain of enterohemorrhagic Escherichia coliO157:H7. (2012)
  • Author(s): Toshi Shimamoto, Mizuki Yamamoto, Ashraf M. Ahmed, Tadashi Shimamoto
  • Organizer: 47th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections
  • Place of Presentation: 千葉市
  • Year and Date: 2012-12-12
• [Presentation] Novel retron in enteropathogenic Yersinia frederiksenii. (2012)
  • Author(s): Rasel Das, Tadashi Shimamoto, Mohammad Arifuzzaman
  • Organizer: 47th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections
  • Place of Presentation: 千葉市
  • Year and Date: 2012-12-12
• [Presentation] Vibrio mimicusの逆転写酵素遺伝子を含むレトロンの構造と機能 (2012)
  • Author(s): 島本 整,田鳥 安紗子,山下 拓未,神本 真紀,島本 敏
  • Organizer: 第46回腸炎ビブリオシンポジウム
  • Place of Presentation: 大分県由布市
  • Year and Date: 2012-11-16
• [Presentation] 腸管出血性大腸菌O157:H7より分離した多剤耐性プラスミドpMDR157の解析. (2012)
  • Author(s): 山本 瑞季,島本 敏,島本 整
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島市
  • Year and Date: 2012-10-20
• [Presentation] 大腸菌臨床株におけるsdsDNA 合成に関与するプラスミド性レトロンの解析 (2012)
  • Author(s): 島本 整,神本 真紀,島本 敏
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-27
• [Presentation] 大腸菌臨床株におけるsdsDNA合成に関与するプラスミド性レトロンの解析 (2012)
  • Author(s): 島本整
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-27
• [Presentation] Novel retron in enteropathogenic Yersinia frederiksenii (2012)
  • Author(s): Rasel Das
  • Organizer: 47th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections
  • Place of Presentation: Chiba, Japan
• [Presentation] Functional characterization of orf540of retron-Vc95 in Vibrio cholerae. (2011)
  • Author(s): Takumi Yamashita, Maki Kamimoto, Toshi Shimamoto, Tadashi Shimamoto
  • Organizer: US-Japan Cooperative Medical Science Program Cholera and Other Bacterial Enteric Infections 46th Conference
  • Place of Presentation: Kolkata, India
  • Year and Date: 2011-12-13
• [Presentation] Functional characterization of orf540 of retron-Vc95 in Vibrio cholerae (2011)
  • Author(s): Tadashi Shimamoto
  • Organizer: US-Japan Cooperative Medical Science Program Cholera and Other Bacterial Enteric Infections 46th Conference
  • Place of Presentation: Kolkata, India
  • Year and Date: 2011-12-13
• [Presentation] 刺身より分離したメチシリン耐性および感受性ブドウ球菌における病原性遺伝子と薬剤耐性遺伝子の解析. (2011)
  • Author(s): 渡邊 亘,藤井 知子,島本 整
  • Organizer: 第64回日本細菌学会中国・四国支部総会
  • Place of Presentation: 岡山市
  • Year and Date: 2011-10-22
• [Presentation] 刺身より分離したメチシリン耐性および感受性ブドウ球菌における病原性遺伝子と薬剤耐性遺伝子の解析 (2011)
  • Author(s): 島本整
  • Organizer: 第64回日本細菌学会中国・四国支部総会
  • Place of Presentation: 岡山市
  • Year and Date: 2011-10-22
• [Presentation] Vibrio choleraeとVibrio mimicusにおけるレトロンの類似性と多様性. (2011)
  • Author(s): 島本 敏,山下 拓未,神本 真紀,島本 整
  • Organizer: 第45回腸炎ビブリオシンポジウム
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2011-10-20
• [Presentation] Vibrio choleraeとVibrio mimicusにおけるレトロンの類似性と多様性 (2011)
  • Author(s): 島本整
  • Organizer: 第45回腸炎ビブリオシンポジウム
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2011-10-20
• [Presentation] Characterization of methicillin-resistant and -susceptible Staphylococci from Japanese retail ready-to-eat raw fish. (2011)
  • Author(s): Ahmed M. Hammad, Wataru Watanabe, Tomoko Fujii, Tadashi Shimamoto
  • Organizer: 2nd ASM-ESCMID Conference on Methicillin-resistant Staphylococci in Animals: Veterinary and Public Health Implications
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2011-09-10
• [Presentation] Characterization of methicillin-resistant and-susceptible Staphylococci from Japanese retail ready-to-eat raw fish (2011)
  • Author(s): Ahmed M.Hammad
  • Organizer: 2nd ASM-ESCMID Conference on Methicillin-resistant Staphylococci in Animals : Veterinary and Public Health Implications
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2011-09-10
• [Presentation] A novel, potentially mobile, retron element (retron-Vc154) in a non-O1, non-O139 serotype strain of Vibrio cholerae. (2011)
  • Author(s): Takumi Yamashita, Miho Kawabata, Kumiko Inouye, Maki Kamimoto, Toshi Shimamoto, Tadashi Shimamoto
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-06
• [Presentation] Functional analysis of orf540of retron-Vc95 inVibrio cholerae (2011)
  • Author(s): Maki Kamimoto, Takumi Yamashita, Toshi Shimamoto, Tadashi Shimamoto
  • Organizer: Functional analysis of orf540of retron-Vc95 inVibrio cholerae
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-06
• [Presentation] Functional analysis of orf540 of retron-Vc95 in Vibrio cholerae (2011)
  • Author(s): Maki Kamimoto
  • Organizer: International Union of Microbiological Societies 2011 Congress・第84回日本細菌学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-06
• [Presentation] A novel, potentially mobile, retron element (retron-Vc154) in a non-01, non-O139 serotype strain of Vibrio cholerae (2011)
  • Author(s): Tadashi Shimamoto
  • Organizer: International Union of Microbiological Societies 2011 Congress・第84回日本細菌学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-06
• [Presentation] コレラ菌の病原性とレトロエレメントとの関係 (2011)
  • Author(s): 島本 整
  • Organizer: 第4回感染病態研究フロンティア
  • Place of Presentation: 吹田市
  • Year and Date: 2011-08-06
• [Presentation] コレラ菌の病原性とレトロエレメントとの関係 (2011)
  • Author(s): 島本整
  • Organizer: 第4回感染病態研究フロンティア
  • Place of Presentation: 吹田市(招待講演)
  • Year and Date: 2011-08-06
• [Presentation] ビブリオ属細菌における新規逆転写酵素遺伝子とレトロンの解析. (2010)
  • Author(s): 山下 拓未,川端 美帆,井上 句美子,神本 真紀,島本 敏,島本 整
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-08
• [Presentation] ビブリオ属細菌における新規逆転写酵素遺伝子とレトロンの解析 (2010)
  • Author(s): 山下拓未
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-08
• [Presentation] A novel retron element and its chromosomal insertion site in Vibrio mimicus (2010)
  • Author(s): Miho Kawabata, Takumi Yamashita, Kumiko Inouye, Maki Kamimoto, Toshi Shimamoto, Tadashi Shimamoto
  • Organizer: 45th US-Japan Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections
  • Place of Presentation: 京都市
  • Year and Date: 2010-12-07
• [Presentation] ビブリオ属細菌ゲノムにおけるレトロンの多様性 (2010)
  • Author(s): 山下 拓未,川端 美帆,井上 句美子,神本 真紀,島本 敏,島本 整
  • Organizer: 第44回腸炎ビブリオシンポジウム
  • Place of Presentation: 秋田市
  • Year and Date: 2010-11-26
• [Presentation] 市販の刺身より分離したBacillus cereusの病原性遺伝子と薬剤感受性の解析 (2010)
  • Author(s): 藤井 知子,渡邊 亘,島本 整
  • Organizer: 第63回日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山市
  • Year and Date: 2010-10-16
• [Presentation] Complete sequence of a novel multidrug resistance plasmid isolated from a clinical strain of enterohemorrhagic Escherichia coli O157:H7
  • Author(s): Tadashi Shimamoto
  • Organizer: 47th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections
  • Place of Presentation: Chiba, Japan
• [Presentation] Vibrio mimicusの逆転写酵素遺伝子を含むレトロンの構造と機能
  • Author(s): 島本 整
  • Organizer: 第46回腸炎ビブリオシンポジウム
  • Place of Presentation: 大分県由布市
• [Presentation] 腸管出血性大腸菌O157:H7より分離した多剤耐性プラスミドpMDR157の解析
  • Author(s): 山本瑞季
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島市
• [Book] 逆転写酵素とレトロン, 腸炎ビブリオ(第IV集) (2013)
  • Author(s): 島本 整,島本 敏
  • Publisher: 近代出版(印刷中)
• [Book] 生命・食・環境のサイエンス (2011)
  • Author(s): 江坂宗春
  • Total Pages: 220
  • Publisher: 共立出版