Clarification of mechanisms how H. pylori virulence factor OipA produces cytokines
Project/Area Number |
22390085
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Bacteriology (including Mycology)
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Research Institution | Oita University |
Principal Investigator |
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Research Collaborator |
GRAHAM David Y ベイラー医科大学, 消化器内科, 教授
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
Keywords | ヘリコバクター・ピロリ / 胃癌 / シグナル伝達 / 微生物 / 感染症 / 菌体病原因子 / 炎症性サイトカイン / 遺伝子変異 |
Research Abstract |
In this study, we tried to clarify the mechanisms how H. pylori virulence factor OipA induce cytokines from gastric epithelial cells. First, we successfully constructed oipA mutants with either of 3 regions which I hypothesized to relate to cytokine expression mutated (including complementation mutants). However all of 3 regions were not strongly involved in cytokine induction from gastric epithelial cells. In experiments using oipA mutants without the function of OipA, we found that OipA is involved in the activation of Paxillin and FoxO related cytokine-induction signaling. In animal models, oipA mutants did not infect mice, suggesting that OipA is involved in the establishment of infection to gastric mucosa.
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Report
(4 results)
Research Products
(103 results)