Live-cell imaging of HIV replication component trafficking and assembly

• Project/Area Number: 22390091
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Kitasato University
• Principal Investigator: MORIKAWA Yuko 北里大学, 大学院・感染制御科学府, 教授 (20191017)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 分子イメージング / 胞内輸送 / アセンブリー / 宿主因子 / リアルタイム / 細胞内輸送

Research Abstract

Live-cell imaging revealed that Gag and GagPol proteins moved together with the transition from “confined” to “diffusive” and ”linear” movements. The linear movement was microtubule-based. The Gag/GagPol with the diffusive and linear movements were partially colocalized with some Rab and SNARE proteins. The RNA containing the Psi site, the signal for genome RNA packaging but not the RNA lacking the signal, was partially merged to Gag/GagPol. In the latter case, Gag/GagPol did not show a long linear movement.

Research Products

• [Journal Article] Trans-packaging of human immunodeficiency virus type 1 genome into Gag virus-like particles in Saccharomyces cerevisiae (2013)
  • Author(s): Tomo N, Goto T, Morikawa Y
  • Journal Title: Microb. Cell Fact Volume: 12 Issue: 1 Pages: 28-28
  • DOI: 10.1186/1475-2859-12-28
  • Peer Reviewed
• [Journal Article] Efavirenz enhances HIV-1 Gag processing at the plasma membrane through Gag-Pol dimerization (2013)
  • Author(s): Sudo S, Haraguchi H, Hirai Y, Gatanaga H, Sakuragi J-I, Momose F, Morikawa Y
  • Journal Title: J Virol Volume: 87 Issue: 6 Pages: 3348-3360
  • DOI: 10.1128/jvi.02306-12
  • Peer Reviewed
• [Journal Article] A large extension to HIV-1 Gag, like Pol, has negative impacts on virion assembly (2012)
  • Author(s): Haraguchi H, Noda T, Kawaoka Y, Morikawa Y
  • Journal Title: PLOS One Volume: 7
  • Peer Reviewed
• [Journal Article] HIV-1 infection ex vivoaccelerates measles virus infection by upregulating signaling lymphocytic activation molecule (SLAM) in CD4+T cells (2012)
  • Author(s): Mitsuki Y, Terahara K, Shibusawa K, Yamamoto T, Tsuchiya T, Ishige M, Kobayashi K, Morikawa Y, Nakayama T, Takeda M, Yanagi Y, Tsunetsugu-Yokota Y
  • Journal Title: J Virol Volume: 86 Issue: 13 Pages: 7227-7234
  • DOI: 10.1128/jvi.06681-11
  • Peer Reviewed
• [Journal Article] Epitope mapping of neutralizing monoclonal antibody in avian influenza A H5N1 virus hemagglutinin (2012)
  • Author(s): Ohkura T, Kikuchi Y, Kono N, Itamura S, Komase K, Momose F, Morikawa Y
  • Journal Title: Biochem.Bioph.Res.Co. Volume: 418 Issue: 1 Pages: 38-43
  • DOI: 10.1016/j.bbrc.2011.12.108
  • Peer Reviewed
• [Journal Article] Viral and cellular requirements for the budding of feline endogenous retrovirus RD-114 (2012)
  • Author(s): Fukuma A, Abe M, Urata S, Yoshikawa R, Morikawa Y, Miyazawa T, Yasuda J
  • Journal Title: Virol.J. Volume: 8 Issue: 1 Pages: 540-540
  • DOI: 10.1186/1743-422x-8-540
  • Peer Reviewed
• [Journal Article] Novel postentry inhibitor of human immunodeficiency virus type 1 replication screened by yeast membrane-associated two-hybrid system (2011)
  • Author(s): Urano E, et al
  • Journal Title: Antimicrob Agents Chemother Volume: 55 Issue: 9 Pages: 4251-4260
  • DOI: 10.1128/aac.00299-11
  • Peer Reviewed
• [Journal Article] The cellular factors Vps18 and Mon2 are required for efficient production of infectious HIV-1 particles (2011)
  • Author(s): Tomita Y, Noda T, Fujii K, Morikawa Y, Kawaoka Y
  • Journal Title: J Virol Volume: 85 Issue: 11 Pages: 5618-5627
  • DOI: 10.1128/jvi.00846-10
  • Peer Reviewed
• [Journal Article] Huwe1, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity (2011)
  • Author(s): Yamamoto SP, Okawa K, Nakano T, Sano K, Ogawa K, Masuda T, Morikawa Y, Koyanagi Y, Suzuki Y
  • Journal Title: Mcirobes Infect Volume: 13 Issue: 4 Pages: 339-349
  • DOI: 10.1016/j.micinf.2010.12.002
  • Peer Reviewed
• [Journal Article] Apical transport of influenza A virus ribonucleoprotein requires Rab11-positive recycling endosome (2011)
  • Author(s): Momose F., et al.
  • Journal Title: PLoS ONE Volume: 6 Issue: 6 Pages: e21123-e21123
  • DOI: 10.1371/journal.pone.0021123
  • Peer Reviewed
• [Journal Article] Cloning and characterization of the antiviral activity of feline Tetherin/BST-2 (2011)
  • Author(s): Fukuma A, Abe M, Morikawa Y, Miyazawa T, Yasuda J
  • Journal Title: PLoS One Volume: 6 Issue: 3 Pages: e18247-e18247
  • DOI: 10.1371/journal.pone.0018247
  • NAID: 120006985155
  • Peer Reviewed
• [Journal Article] Rapid detection of Lassa virus by reverse transcription-loop-mediated isothermal amplification (2011)
  • Author(s): Fukuma A, Kurosaki Y, Morikawa Y, Grolla A, Feldmann H, Yasuda J
  • Journal Title: Microbiol.Immunol. Volume: 55 Issue: 1 Pages: 44-50
  • DOI: 10.1111/j.1348-0421.2010.00286.x
  • NAID: 10031121439
  • Peer Reviewed
• [Journal Article] T cell-based functional cDNA library screening identified SEC13-like 1a carboxy-terminal domain as a negative regulator of human immunodeficiency virus replication (2010)
  • Author(s): Urano E, Ichikawa R, Morikawa Y, Yoshida T, Koyanagi Y, Komano J
  • Journal Title: Vaccine Volume: 28 Pages: B68-B74
  • DOI: 10.1016/j.vaccine.2009.07.084
• [Journal Article] Intracellular localization of human immunodeficiency virus type 1 Gag and GagPol products and virus particle release: Relationship with the Gag-to-GagPol ratio (2010)
  • Author(s): Haraguchi H, Sudo S, Noda T, Momose F, Kawaoka Y, Morikawa Y
  • Journal Title: Microbiol Immunol Volume: 54 Issue: 12 Pages: 734-746
  • DOI: 10.1111/j.1348-0421.2010.00276.x
  • NAID: 10029546490
  • Peer Reviewed
• [Journal Article] T cell-based functional cDNA library screening identified SEC13-like la carboxy-terminal domain as a negative regulator of human immunodeficiency virus replication. (2010)
  • Author(s): Urano E, Ichikawa R, Morikawa Y, Yeshida T, Koyanagi Y, Komano J.
  • Journal Title: Vaccine Volume: 28(S2)
  • Peer Reviewed
• [Journal Article] Involvement of vesicular trafficking system in membrane targeting of the progeny influenza virus genome. (2010)
  • Author(s): Jo S, Kawaguchi A, Takizawa N, Morikawa Y, Momose F, Nagata K.
  • Journal Title: Microbes Infect. Volume: 12 Pages: 1079-1084
  • NAID: 120007137839
  • Peer Reviewed
• [Journal Article] Intracellular localization of human immunodeficiency virus type 1 Gag and GagPol products and virus particle release : Relationship with the Gag-to-GagPol ratio. (2010)
  • Author(s): Haraguchi H, Sudo S, Noda T, Momose F, Kawaoka Y, Morikawa Y.
  • Journal Title: Microbiol.Immunol. Volume: 54 Pages: 734-746
  • NAID: 10029546490
  • Peer Reviewed
• [Journal Article] Rapid detection of Lassa virus by reverse transcription-loop-mediated isothermal amplification.
  • Author(s): Fukuma A, Kurosaki Y, Morikawa Y, Grolla A, Feldmann H, Yasuda J.
  • Journal Title: Microbiol.Immunol. Volume: (in press)
  • NAID: 10031121439
  • Peer Reviewed
• [Journal Article] Huwel, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity.
  • Author(s): Yamamoto SP, Okawa K, Nakano T, Sano K, Ogawa K, Masuda T, Morikawa Y, Koyanagi Y, Suzuki Y.
  • Journal Title: Mcirobes Infect. Volume: (in press)
  • Peer Reviewed
• [Journal Article] The cellular factors Vps18 and Mon2 are required for efficient production of infectious HIV-1 particles.
  • Author(s): Tomita Y, Noda T, Fujii K, Morikawa Y, Kawaoka Y.
  • Journal Title: J.Virol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Cloning and characterization of the antiviral activity of feline Tetherin/BST-2.
  • Author(s): Fukuma A, Abe M, Morikawa Y, Miyazawa T, Yasuda J.
  • Journal Title: PLoS One Volume: (in press)
  • NAID: 120006985155
  • Peer Reviewed
• [Presentation] 生細胞分子イメージングによるHIV-1 Gag/GagPol蛋白の細胞内動態解析 (2012)
  • Author(s): 原口日和、森川裕子
  • Organizer: 第60回日本ウイルス学会学術集会.第60回日本ウイルス学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-13
• [Presentation] ヒト化マウスの麻疹ウイルスベクター評価系への応用 (2012)
  • Author(s): 池野翔太、寺原和孝、石毛真行、鈴木基臣、光木裕也、森川裕子、中山哲夫、横田恭子
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-13
• [Presentation] Efavirenz enhances HIV-1 Gag processing on the plasma membrane through GagPol dimerization (2012)
  • Author(s): Sudo S, Haraguchi H, Hirai Y, Gatanaga H, Sakuragi J-I, Momose F, Morikawa Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: 米国NY
  • Year and Date: 2012-05-23
• [Presentation] Efavirenz enhances HIV-1 Gag processing on the plasma membrane through GagPol dimerization (2012)
  • Author(s): Sudo S, Haraguchi H, Hirai Y, Gatanaga H, Sakuragi J-I, Momose F, Morikawa Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: New York
• [Presentation] 生細胞分子イメージングによるHIV-1 Gag/GagPol蛋白の細胞内動態解析 (2012)
  • Author(s): 原口日和、森川裕子
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] ヒト化マウスの麻疹ウイルスベクター評価系への応用 (2012)
  • Author(s): 池野翔太、寺原和孝、石毛真行、鈴木基臣、光木裕也、森川裕子、中山哲夫、横田恭子
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] インフルエンザウイルスvRNP陽性リサイクリングエンドソームの極性輸送に関する宿主因子の機能解析 (2012)
  • Author(s): 百瀬文隆、森川裕子
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] インフルエンザウイルス膜タンパク質の協調的極性輸送機構の解析 (2012)
  • Author(s): 大倉喬、齋藤桃子、百瀬文隆、森川裕子
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] HIV infection enhances the susceptibility of T cells to measles virus infection by upregulating signaling lymphocyte activation molecule (SLAM) expression (2011)
  • Author(s): Mitsuki Y, Shibusawa K, Terahara K, Kobayashi K, Morikawa Y, Nakayama T, Takeda M, Yanagi Y, Tsunetsugu Yokota Y
  • Organizer: 第15回国際ウイルス学会
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-15
• [Presentation] The Pol region of human immunodeficiency virus GagPol negatively regulates its membrane binding and particle assembly (2011)
  • Author(s): Haraguchi H, Noda T, Kawaoka, Y, Morikawa Y
  • Organizer: 第15回国際ウイルス学会
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Presentation] Establishment of feline cell line suitable for vaccine manufacturing (2011)
  • Author(s): Fukuma A, Morikawa Y, Miyazawa T, Yasuda J
  • Organizer: 第15回国際ウイルス学会
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Presentation] Apical transport of influenza A virus ribonucleoprotein requires Rab11-positive recycling endosome (2011)
  • Author(s): Momose F, Sekimoto T, Ohkura T, Jo S, Kawaguchi A, Nagata K, Morikawa Y
  • Organizer: 第15回国際ウイルス学会
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Presentation] Epitope mapping of neutralizing antibody in avian influenza A H5N1 virus hemagglutinin and construction of its single-chain variable fragment (2011)
  • Author(s): Ohkura T, Kikuchi Y, Kono N, Itamura S, Komase K, Momose F, Morikawa Y
  • Organizer: 第15回国際ウイルス学会
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Presentation] Human immunodeficiency virus GagPol negatively regulates its membrane binding and particle assembly (2011)
  • Author(s): Haraguchi H, Noda T, Kawaoka, Y, Morikawa Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: 米国NY
  • Year and Date: 2011-05-24
• [Presentation] Human immunodeficiency virus GagPol negatively regulates its membrane binding and particle assembly (2011)
  • Author(s): Haraguchi H, Noda T, Kawaoka, Y, Morikawa Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: New York
  • Year and Date: 2011-05-24
• [Presentation] Rab蛋白質を指標としたHIV Gag蛋白質の細胞内輸送経路の解析 (2011)
  • Author(s): 中山順之、百瀬文隆、森川裕子
  • Organizer: 第34回日本分子生物学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Rab11陽性リサイクリングエンドソームによるインフルエンザウイルスゲノム-蛋白質複合体の極性輸送 (2011)
  • Author(s): 百瀬文隆、関本哲也、大倉喬、森川裕子
  • Organizer: 第34回日本分子生物学会学術集会
  • Place of Presentation: 横浜
• [Presentation] インフルエンザウイルス子孫vRNP複合体の極性輸送を担う輸送小胞および宿主因子の同定 (2010)
  • Author(s): 百瀬文隆、関本哲也、大倉喬、森川裕子
  • Organizer: 第33回日本分子生物学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-12-07
• [Presentation] ネコTetherin/BST-2によるRD-114ウイルスの産生抑制 (2010)
  • Author(s): 福間藍子, 阿部真澄, 宮沢孝幸, 森川裕子, 安田二朗
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-09
• [Presentation] HIV-1 Gag/GagPol蛋白の発現比率と相関する成熟産物の細胞内局在と粒子形成効率 (2010)
  • Author(s): 原口日和, 周東翔, 森川裕子.
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-08
• [Presentation] ESCRT-0エンドソーム因子HRSの共発現により、BST-2はエンドサイトーシスされる (2010)
  • Author(s): 星野悠, 奥長浩之, 森川裕子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-08
• [Presentation] インフルエンザウイルス子孫vRNP複合体の極性輸送を担う輸送小胞および宿主因子の探索 (2010)
  • Author(s): 百瀬文隆, 大倉喬, 森川裕子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-08
• [Presentation] HN-1 Gagを標的とする低分子化合物BMMPによるウイルスエントリー阻害機構 (2010)
  • Author(s): 浦野恵美子, 倉持紀子, 市川玲子, 宮内浩典, 供田洋, 武部豊, 駒野淳, 森川裕子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07
• [Presentation] インフルエンザウイルスHAの極性輸送機構の解析 (2010)
  • Author(s): 大倉喬, 百瀬文隆, 森川裕子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07
• [Presentation] Live-cell imaging of human immunodeficiency virus Gag and Gag-Pol trafficking (2010)
  • Author(s): Haraguchi H, Morikawa Y
  • Organizer: 第10回感染症免疫フォーラム
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Presentation] Overexpression of HRS induces BST-2 downregulation through its clathrin-binding domain (2010)
  • Author(s): Hoshino Y, Okunaga H, Morikawa Y
  • Organizer: 第10回感染症免疫フォーラム
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Presentation] Live-cell imaging of human immunodeficiency virus Gag and Gag-Pol trafficking. (2010)
  • Author(s): Haraguchi H, Morikawa Y
  • Organizer: 第10回感染症免疫フォーラム
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Presentation] Overexpression of HRS induces BST-2 downregulation through its clathrin-binding domain. (2010)
  • Author(s): Hoshino Y, Okunaga H, Morikawa Y.
  • Organizer: 第10回感染症免疫フォーラム
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Presentation] Anovel postentry inhibitor of human immunodeficiency virus type 1 replication screened by yeast membrane-associated two-hybrid system. (2010)
  • Author(s): Urano E, Kuramochi N, Miyauchi K, Ishikawa R, Tomoda H, Takebe Y, Komano J, Morikawa Y.
  • Organizer: 第10回感染症免疫フォーラム
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Presentation] Live cell imaging of influenza A virus hemagglutinin : dynamics on the apical plasma membrane and intracellular trafficking. (2010)
  • Author(s): Ohkura T, Momose F, Morikawa Y.
  • Organizer: 第10回感染症免疫フォーラム
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Presentation] Inhibitor of HIV-1 Gag assembly screened by yeast membrane-associated system. (2010)
  • Author(s): Urano E, Kuramochi N, Tomoda H, Takebe Y, Miyauchi K, Komano J, Morikawa Y.
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: New York, USA
  • Year and Date: 2010-05-27
• [Presentation] Modulation of HIV-1 infection at late phase by an integrase-interactor Huwel. (2010)
  • Author(s): Yamamoto SP, Okawa K, Masuda T, Morikawa Y, Koyanagi Y, Suzuki Y.
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: New York, USA
  • Year and Date: 2010-05-27
• [Presentation] Inhibitor of HIV-1 Gag assembly screened by yeast membrane-associated system (2010)
  • Author(s): Urano E, Kuramochi N, Tomoda H, Takebe Y, Miyauchi K, Komano J, Morikawa Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: 米国NY
• [Presentation] Modulation of HIV-1 infection at late phase by an integrase-interactor Huwe1 (2010)
  • Author(s): Yamamoto SP, Okawa K, Masuda T, Morikawa Y, Koyanagi Y, Suzuki Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: 米国NY
• [Remarks]
  • URL: http://www.kitasato-u.ac.jp/lisci/life/chart/LSI-lab12.html
• [Remarks]
  • URL: http://www.kitasato-u.ac.jp/lisci/labo/ViralInfection2/index.html