Development of selective chemiluminescence assay for biologicalquinones and its application to clinical investigation
| Project/Area Number |
22390116
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
KURODA Notaka 長崎大学, 大学院・医歯薬学総合研究科, 教授 (50234612)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHYAMA Kaname 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (50437860)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
|---|
| Keywords | 臨床化学 / 生体キノン / 化学発光 / ハイスループット分析 / 高速液体クロマトグラフィー / キノン / 活性酸素 / 酸化還元サイクル / HPLC / キノン付加体 / 化学発光反応 / マイクロプレートリーダー / ビタミンK / 関節リウマチ |
|---|
| Research Abstract |
Quinones are interesting compounds which have several important roles in biological functions such as mitochondrial electron transport chain and blood coagulation. On the other hand, quinones are regarded as a class of toxins which can cause a variety of hazardous effects on living health by the covalent binding to the protein and DNA, or the generation of reactive oxygen species through the redox cycle in biological system. In this study, sensitive and selective determination methods for biological quinones were developed based on the specific photoreaction and redox reaction of quinone and applied to biological samples to evaluate the usefulness of the developed method in clinical investigation.
|
Report
(4 results)
Research Products
(73 results)
-
-
-
-
-
-
-
-
-
[Journal Article] 関節リウマチ(RA)患者の酸化ストレス度:ものさし"GAP比"の導入とその応用について-Sampled Studiesのアプローチ-2012
Author(s)
今里孝宏,植木幸孝,平方尚之,黒田直敬,岸川直哉,矢野正生,柴 輝男,大西紀子,井越尚子,下村弘治,足立哲夫,鈴木直季,谷山松雄,前畑英介
-
Journal Title
生物試料分析
Volume: 35 (3)
Pages: 225-33
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Yamaguchi S, Kishikawa N, Ohyama K, Ohba Y, Kohno M, Masuda T, Takadate A, Nakashima K, Kuroda N2010
Author(s)
Yamaguchi S, Kishikawa N, Ohyama K, Ohba Y, Kohno M, Masuda T, Takadate A, Nakashima K, Kuroda N
-
Journal Title
Anal Chim Acta
Volume: 665
Pages: 74-8
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
