Establishment and application of the strategies for the discovery of biomarker proteins and peptides including species bound to albumin in serum/plasma

• Project/Area Number: 22390117
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kitasato University
• Principal Investigator: KODERA Yoshio 北里大学, 理学部物理学科, 准教授 (60265733)
• Co-Investigator(Kenkyū-buntansha): SATO Yuichi 北里大学, 医療衛生学部医療検査学科, 教授 (30178793) ; MATSUMOTO Kazumasa 北里大学, 医学部泌尿器科, 講師 (70306603) ; IWAMURA Masatsugu 北里大学, 医学部泌尿器科, 教授 (20176564)
• Co-Investigator(Renkei-kenkyūsha): MAEDA Tadakazu 北里大学, 名誉教授 (90265728)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2010: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: 臨床検査医学 / 血中診断マーカー / 血清 / タンパク質 / ペプチド / 診断マーカー / アルブミン結合成分 / アルブミン

Research Abstract

We developed a differential solubilization (DS) method to extract low-molecular-weight proteins/peptides containing many peptides bound to carrier proteins such as albumin in serum with good reproducibility and yield. Using the DS method we performed high-quality comparative analyses of more than 1500 peptides from 1μL of serum samples. Furthermore, we optimized the DS method to selectively enrich peptides less than 6 kDa. The modified DS method was combined with LC-MS and Two candidate peptides of renal cell carcinoma were discovered.

Research Products

• [Journal Article] Enhanced recovery of lyophilized peptides in shotgun proteomics by using an LC-ESI-MS compatible surfactant (2013)
  • Author(s): Kawashima Y, Takahashi N, Satoh M, Saito T, Kado S, Nomura F, Matsumoto H, Kodera Y
  • Journal Title: Proteomics Volume: 13(5) Pages: 751-5
  • Peer Reviewed
• [Journal Article] Establishment and application of a high-quality comparative analysis strategy for the discovery and small-scale validation of low-abundance biomarker peptides in serum based on an optimized novel peptide extraction method (2013)
  • Author(s): Saito T, Kawashima Y, Minamida S, Matsumoto K, Araki K, Matsui T, Satoh M, Nomura F, Iwamura M, Maeda T, Baba S, Kodera Y
  • Journal Title: Journal of Electrophoresis Volume: 57 Issue: 1 Pages: 1-9
  • DOI: 10.2198/jelectroph.57.1
  • NAID: 130003364779
  • ISSN: 1349-9394, 1349-9408
  • Peer Reviewed
• [Journal Article] Application of quantitative proteomic analysis using tandem mass tags for discovery and identification of novel biomarkers in periodontal disease (2013)
  • Author(s): Tsuchida S, Satoh M, Kawashima Y, Sogawa K, Kado S, Sawai S, Nishimura M, Ogita M, Takeuchi Y, Kobyashi H, Aoki A, Kodera Y, Matsushita K, IzumiY, Nomura F
  • Journal Title: Proteomics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Serum monomeric α2-macroglobulin as a clinical biomarker in diabetes (2013)
  • Author(s): Takada T, Kodera Y, Matsubara M, Kawashima Y, Maeda T, Fujita Y, Shichiri M
  • Journal Title: Atherosclerosis Volume: 228(1) Pages: 270-6
  • Peer Reviewed
• [Journal Article] Cyclic sample pooling using two-dimensional liquid chromatography system enhancescoverage in shotgun proteomics (2013)
  • Author(s): Kawashima Y, Satoh M, Saito T, Matsui T, Nomura F, Matsumoto H, Kodera Y
  • Journal Title: Biomed Chromatogr Volume: 27(6) Pages: 691-4
  • Peer Reviewed
• [Journal Article] Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy (2013)
  • Author(s): Ichimura K, Kawashima Y, Nakamura T, Powell R, Hidoh Y, Terai S, Sakaida I,Kodera Y, Tsuji T, Ma JX, Sakai T, Matsumoto H, Obara T
  • Journal Title: Biochem Biophys Res Commun Volume: 431(4) Pages: 712-7
  • Peer Reviewed
• [Journal Article] Integrated view of the human chromosome X-centric proteome project (2013)
  • Author(s): Yamamoto T, Nakayama K, Hirano H,Tomonaga T, Ishihama Y, Yamada T, Kondo T, Kodera Y, Sato Y, Araki N, Mamitsuka H, Goshima N
  • Journal Title: J Proteome Res Volume: 12(1) Pages: 58-61
  • Peer Reviewed
• [Journal Article] Preanalytical evaluation of serum 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 measurements using LC-MS/MS (2013)
  • Author(s): Mochizuki A, Kodera Y, Saito T, Satoh M, Sogawa K, Nishimura M, Seimiya M,Kubota M, Nomura F
  • Journal Title: Clin Chim Acta Volume: 420 Pages: 114-20
  • Peer Reviewed
• [Journal Article] Enhanced recovery of lyophilized peptides in shotgun proteomics by using an LC-ESI-MS compatible surfactant (2013)
  • Author(s): Kawashima Y, Takahashi N, Satoh M, Saito T, Kado S, Nomura F, Matsumoto H, Kodera Y
  • Journal Title: Proteomics Volume: 13 Issue: 5 Pages: 751-755
  • DOI: 10.1002/pmic.201200462
  • Peer Reviewed
• [Journal Article] Cyclic sample pooling using two-dimensional liquid chromatography system enhances coverage in shotgun proteomics (2013)
  • Author(s): Kawashiraa Y, Satoh M, Saito T, Matsui T, Nomura F, Matsumoto H, Kodera Y
  • Journal Title: Biomedical Chromatography Volume: 27 Issue: 6 Pages: 691-694
  • DOI: 10.1002/bmc.2864
  • Peer Reviewed
• [Journal Article] Preanalytical evaluation of serum 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 measurements using LC-MS/MS. (2013)
  • Author(s): Mochizuki A, Kodera Y, Saito T, Satoh M, Sogawa K, Nishimura M, Seimiya M,Kubota M, Nomura F.
  • Journal Title: Clin Chim Acta. Volume: 420 Pages: 114-120
  • DOI: 10.1016/j.cca.2012.10.041
  • Peer Reviewed
• [Journal Article] Serum monomeric α2-macroglobulin as a clinical biomarker in diabetes. (2013)
  • Author(s): Takada T, Kodera Y, Matsubara M, Kawashima Y, Maeda T, Fujita Y, Shichiri M.
  • Journal Title: Atherosclerosis. Volume: 228 Issue: 1 Pages: 270-276
  • DOI: 10.1016/j.atherosclerosis.2013.02.035
  • Peer Reviewed
• [Journal Article] Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy (2013)
  • Author(s): Ichimura K, Kawashima Y, Nakamura T, Powell R, Hidoh Y, Terai S, Sakaida l, Kodera Y, T suji T, Ma JX, Sakai T, Matsumoto H, Obara T
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 431 Issue: 4 Pages: 712-717
  • DOI: 10.1016/j.bbrc.2013.01.053
  • Peer Reviewed
• [Journal Article] Integrated view of the human chromosome X-centric proteome project. (2013)
  • Author(s): Yamamoto T, Nakayama K, Hirano H, Tomonaga T, Ishihama Y, Yamada T, Kondo T,Kodera Y, Sato Y, Araki N, Mamitsuka H, Goshima N.
  • Journal Title: J Proteome Res. Volume: 12 Issue: 1 Pages: 58-61
  • DOI: 10.1021/pr300844p
  • Peer Reviewed
• [Journal Article] Angiotensin-converting enzyme inhibition curbs tyrosine nitration of mitochondrial proteins in the renal cortex during the early stage of diabetes mellitus in rats. (2013)
  • Author(s): Ishii N, Carmines PK, Yokoba M, Imaizumi H, Ichikawa T, Ikenagasa H, Kodera Y,Oh-Ishi M, Aoki Y, Maeda T, Takenaka T, Katagiri M.
  • Journal Title: Clin Sci (Lond). Volume: 124 Issue: 8 Pages: 543-552
  • DOI: 10.1042/cs20120251
  • Peer Reviewed
• [Journal Article] A strategy for large-scale phosphoproteomics and SRM-based validation of human breast cancer tissue samples (2012)
  • Author(s): Narumi R, Murakami T, Kuga T, Adachi J, Shiromizu T, Muraoka S, Kume H, Kodera Y, Matsumoto M, Nakayama K, Miyamoto Y, Ishitobi M, Inaji H, Kato K, Tomonaga T
  • Journal Title: J Proteome Res Volume: 11(11) Pages: 5311-22
  • Peer Reviewed
• [Journal Article] Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples (2012)
  • Author(s): Muraoka S, Kume H, Watanabe S, Adachi J, Kuwano M, Sato M, Kawasaki N, Kodera Y, Ishitobi M, Inaji H, Miyamoto Y, Kato K, Tomonaga T
  • Journal Title: J Proteome Res Volume: 11(8) Pages: 4201-10
  • Peer Reviewed
• [Journal Article] Proteomic analysis of gingival crevicular fluid for discovery of novel periodontal disease markers (2012)
  • Author(s): Tsuchida S, Satoh M, Umemura H, Sogawa K, Kawashima Y, Kado S, Sawai S,Nishimura M, Kodera Y, Matsushita K, Nomura F
  • Journal Title: Proteomics Volume: 12(13) Pages: 2190-202
  • Peer Reviewed
• [Journal Article] Potential ofclassification of cancer by multiple discriminant analysis for relationship between cancer and expression of human cellular phosphoprotein (2012)
  • Author(s): Motofuji Y, Saito A, Koike M, Kodera Y, Maeda T, Komatsu H
  • Journal Title: Biomed Res Volume: 33(2) Pages: 139-43
  • Peer Reviewed
• [Journal Article] Plectin promotes migration and invasion of cancer cells and is a novel prognostic marker for head and neck squamous cell carcinoma (2012)
  • Author(s): Katada K, Tomonaga T, Satoh M, Matsushita K, Tonoike Y, Kodera Y, Hanazawa T, Nomura F, Okamoto Y
  • Journal Title: Jproteomics Volume: 75(6) Pages: 1803-15
  • Peer Reviewed
• [Journal Article] A strategy for large-scale phosphoproteomics and SRM-based validation of human breast cancer tissue samples. (2012)
  • Author(s): Narumi R, Murakami T, Kuga T, Adachi J, Shiromizu T, Muraoka S, Kume H, Kodera Y, Matsumoto M, Nakayama K, Miyamoto Y, Ishitobi M, Inaji H, Kato K, Tomonaga T.
  • Journal Title: J Proteome Res. Volume: 11 Issue: 11 Pages: 5311-5322
  • DOI: 10.1021/pr3005474
  • Peer Reviewed
• [Journal Article] The application of a three-step serum proteome analysis for the discovery and identification of novel biomarkers of hepatocellular carcinoma. (2012)
  • Author(s): Kimura A, Sogawa K, Satoh M, Kodera Y, Yokosuka O, Tomonaga T, Nomura F.
  • Journal Title: Int J Proteomics. Volume: 2012: Pages: 623190-623190
  • DOI: 10.1155/2012/623190
  • Peer Reviewed
• [Journal Article] Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples. (2012)
  • Author(s): Muraoka S, Kume H, Watanabe S, Adachi J, Kuwano M, Sato M, Kawasaki N, Kodera Y, Ishitobi M, Inaji H, Miyamoto Y, Kato K, Tomonaga T.
  • Journal Title: J Proteome Res. Volume: 11 Issue: 8 Pages: 4201-4210
  • DOI: 10.1021/pr300322q
  • Peer Reviewed
• [Journal Article] Proteomic analysis of gingival crevicular fluid for discovery of novel periodontal disease markers. (2012)
  • Author(s): Tsuchida S
  • Journal Title: Proteomics. Volume: 12(13) Issue: 13 Pages: 2190-202
  • DOI: 10.1002/pmic.201100655
  • Peer Reviewed
• [Journal Article] Potential of classification of cancer by multiple discriminant analysis for relationship between cancer and expression of human cellular phosphoprotein. (2012)
  • Author(s): Motofuji Y, Saito A, Koike M, Kodera Y, Maeda T, Komatsu H.
  • Journal Title: Biomed Res. Volume: 33 Pages: 139-143
  • NAID: 130004470809
  • Peer Reviewed
• [Journal Article] Plectin promotes migration and invasion of cancer cells and is a novel prognostic marker for head and neck squamous cell carcinoma. (2012)
  • Author(s): Katada K, Tomonaga T, Satoh M, Matsushita K, Tonoike Y, Kodera Y, Hanazawa T,Nomura F, Okamoto Y.
  • Journal Title: J Proteomics. Volume: 75 Issue: 6 Pages: 1803-1815
  • DOI: 10.1016/j.jprot.2011.12.018
  • Peer Reviewed
• [Journal Article] 14-3-3 protein beta/alpha as a urinary biomarker for renal cell carcinoma: proteomic analysis of cyst fluid (2011)
  • Author(s): inamida S, Iwamura M, Kodera Y, Kawashima Y, Tabata K, Matsumoto K, Fujita T, Satoh T, Maeda T, Baba S
  • Journal Title: Anal Bioanal Chem Volume: 401(1) Pages: 245-52
  • Peer Reviewed
• [Journal Article] Development of a sandwich ELISA for the 5.9-kDa fibrinogen alpha C chain fragment detected by serum proteome analysis (2011)
  • Author(s): Noda K, Sogawa K, Kikuchi W, Kiyokawa I, Miura T, Kojima R, Katayama K,Kodera Y, Nomura F
  • Journal Title: Proteomics Clin Appl Volume: 5(3-4) Pages: 141-6
  • Peer Reviewed
• [Journal Article] The measurement of a fibrinogen α C-chain 5.9 kDa fragment (FIC 5.9) using MALDI-TOF MS and a stable isotope-labeled peptide standard dilution (2011)
  • Author(s): Sogawa K, Kodera Y, Noda K, Ishizuka Y, Yamada M, Umemura H, Maruyama K,Tomonaga T, Yokosuka O, Nomura F
  • Journal Title: Clin Chim Acta Volume: 412(11-12) Pages: 1094-9
  • Peer Reviewed
• [Journal Article] Profilin 1 overexpression in renal cell carcinoma (2011)
  • Author(s): Minamida S, Iwamura M, Kodera Y, Kawashima Y, Ikeda M, Okusa H, Fujita T, Maeda T, Baba S
  • Journal Title: Int J Urol Volume: 18(1) Pages: 63-71
  • NAID: 10027902072
  • Peer Reviewed
• [Journal Article] Increased serum levels of pigment epithelium-derived factor by excessive alcohol consumption-detection and identification by a three-step serum proteome analysis (2011)
  • Author(s): Sogawa K, Kodera Y, Satoh M, Kawashima Y, Umemura H, Maruyama K, Takizawa H, Yokosuka O, Nomura F
  • Journal Title: Alcohol Clin Exp Res Volume: 35(2) Pages: 211-7
  • Peer Reviewed
• [Journal Article] Serum Level of Fibrinogen Aα Chain Fragment Increases in Chronic Thromboembolic Pulmonary Hypertension (2011)
  • Author(s): Yano T, Sogawa K, Umemura H, Sakao S, Kasahara Y, Tanabe N, Kodera Y, Takiguchi Y, Tatsumi K, Nomura F
  • Journal Title: Circ J.75 Volume: 75 Pages: 2675-2682
  • NAID: 10030032528
  • Peer Reviewed
• [Journal Article] The application of a three-step proteome analysis for identification of new biomarkers of pancreatic cancer (2011)
  • Author(s): Abulaizi M, Tomonaga T, Satoh M, Sogawa K, Matsushita K, Kodera Y, Obul J, Takano S, Yoshitomi H, Miyazaki M, Nomura F
  • Journal Title: Int J Proteomics Volume: 2011 Pages: 1-13
  • Peer Reviewed
• [Journal Article] 14-3-3 protein beta/alpha as a urinary biomarker for renal cell carcinoma : proteomic analysis of cyst fluid (2011)
  • Author(s): Minamida S, Iwamura M, Kodera Y, Kawashima Y, Tabata K, Matsumoto K, Fujita T, Satoh T, Maeda T, Baba S
  • Journal Title: Anal Bioanal Chem Volume: 401 Pages: 245-252
  • Peer Reviewed
• [Journal Article] Development of a sandwich ELISA for the 5.9-kDa fibrinogen alpha C chain fragment detected by serum proteome analysis (2011)
  • Author(s): Noda K, Sogawa K, Kikuchi W, Kiyokawa I, Miura T, Kojima R, Katayama K, Kodera Y, Nomura F
  • Journal Title: Proteomics Clin Appl Volume: 5 Issue: 3-4 Pages: 141-146
  • DOI: 10.1002/prca.201000127
  • Peer Reviewed
• [Journal Article] The measurement of a fibrinogen α C-chain 5.9 kDa fragment (FIC 5.9) using MALDI-TOF MS and a stable isotope-labeled peptide standard dilution (2011)
  • Author(s): Sogawa K, Kodera Y, Noda K, Ishizuka Y, Yamada M, Umemura H, Maruyama K, Tomonaga T, Yokosuka O, Nomura F
  • Journal Title: Clin Chim Acta Volume: 412 Pages: 1094-1099
  • Peer Reviewed
• [Journal Article] Identification of a high molecular weight kininogen fragment as a marker for early gastric cancer by serum proteome analysis (2011)
  • Author(s): Umemura H, Togawa A, Sogawa K, Satoh M, Mogushi K, Nishimura M, Matsushita K, Tanaka H, Takizawa H, Kodera Y, Nomura F
  • Journal Title: J Gastroenterol Volume: 46 Pages: 577-585
  • NAID: 10029028943
  • Peer Reviewed
• [Journal Article] Increased serum levels of pigment epithelium-derived factor by excessive alcohol consumption-detection and identification by a three-step serum proteome analysis (2011)
  • Author(s): Sogawa K, Kodera Y, Saton M, Kawashima Y, Umemura H, Maruyama K, Takizawa H, Yokosuka O, Nomura F
  • Journal Title: Alcohol Clin Exp Res Volume: 35 Pages: 211-217
  • Peer Reviewed
• [Journal Article] Effects of humidity on the dried-dropletsample preparation for MALDI-TOF MS peptide profiling (2010)
  • Author(s): Umemura H, Kodera Y, Nomura F
  • Journal Title: Clin Chim Acta Volume: 411(23-24) Pages: 2109-11
  • Peer Reviewed
• [Journal Article] High-yield peptide-extraction method for the discovery of subnanomolar biomarkers from small serum samples (2010)
  • Author(s): Kawashima Y, Fukutomi T, Tomonaga T, Takahashi H, Nomura F, Maeda T, Kodera Y
  • Journal Title: J Proteome Res Volume: 9(4) Pages: 1694-705
  • Peer Reviewed
• [Journal Article] Proteomic study of sera from patients with bladder cancer:usefulness of S100A8 and S100A9 proteins (2010)
  • Author(s): Minami S, Sato Y, Matsumoto T, Kageyama T, Kawashima Y, Kodera Y, Ishii J, Matsumoto K, Nagashio R, Okayasu I
  • Journal Title: Cancer GenomicsProteomics Volume: 7(4) Pages: 181-9
  • Peer Reviewed
• [Journal Article] High-yield peptide-extraction method for the discovery of subnanomolar biomarkers from small serum samples. (2010)
  • Author(s): Kawashima Y, Fukutomi F, Tomonaga T, Takahashi H, Nomura F, Maeda T, Kodera Y
  • Journal Title: J.Proteome Research Volume: 9 Pages: 1694-1705
  • Peer Reviewed
• [Journal Article] Effects of humidity on the dried-droplet sample preparation for MALDI-TOF MS peptide profiling. (2010)
  • Author(s): Umemura H, Kodera Y, Nomura F.
  • Journal Title: Clinica Chimica Acta Volume: 411 Pages: 2109-2111
  • Peer Reviewed
• [Journal Article] Proteomic study of sera from patients with bladder cancer-Usefulness of S100A8 and S100A9 proteins (2010)
  • Author(s): Minami S, Sato Y, Matsumoto T, Kageyama T, Kawashima Y, Kodera Y, Ishii J, Matsumoto K, Nagashio R, Okayasu I.
  • Journal Title: Cancer Genomics Proteomics Volume: 7 Pages: 181-189
  • Peer Reviewed
• [Journal Article] Serum levels of complement C4 fragments correlate with disease activity in multiple sclerosis : proteomic analysis. (2010)
  • Author(s): Sawai S, Umemura H, Mori M, Satoh M, Hayakawa S, Kodera Y, Tomonaga T, Kuwabara S, Nomura F.
  • Journal Title: J Neuroimmunol Volume: 218 Pages: 112-115
  • Peer Reviewed
• [Journal Article] Application of Fourier-transform infrared (FT-IR) spectroscopy for simple and easy determination of chylomicron-triglyceride and very low density lipoprotein-triglyceride. (2010)
  • Author(s): Sato K, Seimiya M, Kodera Y, Kitamura A, Nomura F
  • Journal Title: Clinica Chimica Acta Volume: 411 Pages: 285-290
  • Peer Reviewed
• [Journal Article] A new possible lung cancer marker: VGF detection from the conditioned medium of pulmonary large cell neuroendocrine carcinoma-derived cells using secretome analysis (2009)
  • Author(s): Matsumoto T, Kawashima Y, Nagashio R, Kageyama T, Kodera Y, Jiang SX, Okayasu I, Kameya T, Sato Y
  • Journal Title: Int J Biol Markers Volume: 24(4) Pages: 282-5
  • Peer Reviewed
• [Presentation] 血中診断マーカーペプチド獲得を目指したペプチドーム解析法の包括的な開発 (2012)
  • Author(s): 小寺義男
  • Organizer: 第19回遺伝子診療学会/シンポジウム(招待講演)
  • Place of Presentation: 千葉
  • Year and Date: 2012-07-28
• [Presentation] 血中診断マーカーペプチド獲得を目指した ペプチドーム解析法の包括的な開発 (2012)
  • Author(s): 小寺義男
  • Organizer: 第10回日本プロテオーム学会(JHUPO)年会(シンポジウム)
  • Place of Presentation: 東京
  • Year and Date: 2012-07-27
• [Presentation] 血中疾患マーカーペプチド獲得を目指した包括的なアプローチ (2012)
  • Author(s): 小寺義男
  • Organizer: 東北大学医学部・最先端学術セミナー(招待講演)
  • Place of Presentation: 東北大学医学部
  • Year and Date: 2012-06-15
• [Presentation] High quality method for discovery and verification of low abundance biomarker peptides in serum (2012)
  • Author(s): "Yoshio Kodera Tatsuya Saito, Yusuke Kawashima, Satoru Minamida, Kazumasa Matsumoto, Keita Araki, Takashi Matsui, Mamoru Satoh, Fumio Nomura,Masatsugu Iwamura, Shiro Baba ,Tadakazu Maeda
  • Organizer: AOHUPO (Asia Oceania HUman Proteomics Organization)
  • Place of Presentation: 北京／中国
• [Presentation] 血中診断マーカーペプチド獲得を目指した ペプチドーム解析法の包括的な開発 (2012)
  • Author(s): "小寺義男，川島祐介，斎藤達也，佐藤守 ，曽川一幸 ，前田忠計 ，朝長毅 ，野村文夫
  • Organizer: 第10回日本プロテオーム学会（JHUPO）年会／シンポジウム
  • Place of Presentation: 日本科学未来館(東京）
  • Invited
• [Presentation] 血中診断マーカーペプチド獲得を目指したペプチドーム解析法の包括的な開発 (2012)
  • Author(s): 小寺義男
  • Organizer: 第19回遺伝子診療学会／シンポジウム
  • Place of Presentation: 三井ガーデンホテル千葉
  • Invited
• [Presentation] Comprehensive approach to develop diagnostic biomarker peptides in serum –Establishment of discovery and validation methods targeting low-abundance peptides– (2012)
  • Author(s): Yoshio Kodera,Tatsuya Saitoh,Yusuke Kawashima, Kazuyuki Sogawa, Satoru Minamida, Kazumasa Matsumoto, Masatsugu Iwamura, Shiro Baba,Tadakazu Maeda,Takeshi Tomonaga,Fumio Nomura
  • Organizer: HUPO 2012 11th World Congress
  • Place of Presentation: ボストン（USA)
• [Presentation] 血中診断マーカーペプチド提案を目指した 探索ならびに多検体評価法の包括的な開発 (2012)
  • Author(s): "小寺義男，川島祐介，斎藤達也，佐藤守 ，曽川一幸 ，前田忠計 ，朝長毅，野村文夫
  • Organizer: 第85回 日本生化学会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡
• [Presentation] 血中診断マーカーペプチド獲得を目指した包括的なアプローチ (2011)
  • Author(s): 小寺義男
  • Organizer: 第2回バイオビジネス・スタートアップ -バイオマーカー研究と診断への応用-(招待講演)
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-20
• [Presentation] 血中診断マーカーペプチド獲得を目指した包括的なアプローチ (2011)
  • Author(s): 小寺義男
  • Organizer: 第2回バイオビジネス・スタートアップ-バイオマーカー研究と診断への応用-
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-20
• [Presentation] 血中診断マーカーペプチド開発に向けた様々なアプローチ (2011)
  • Author(s): 小寺義男
  • Organizer: 第224回液体クロマトグラフィー研究懇談会 (招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2011-10-25
• [Presentation] 血中診断マーカーペプチド開発に向けた様々なアプローチ (2011)
  • Author(s): 小寺義男
  • Organizer: 第244回液体クロマトグラフィー研究懇談会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-25
• [Presentation] 共同利用における LC-MS 解析 (2011)
  • Author(s): 小寺義男
  • Organizer: 第84回日本生化学会・フォーラム(フォーラム企画、発表)
  • Place of Presentation: 東京
  • Year and Date: 2011-09-23
• [Presentation] 共同利用におけるLC-MS解析 (2011)
  • Author(s): 小寺義男
  • Organizer: 第84回日本生化学会・フォーラム
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] 血中診断マーカー獲得を目指した定量的プロテオミクス (2011)
  • Author(s): 小寺義男
  • Organizer: 日本プロテオーム学会 2011 大会(シンポジウム)
  • Place of Presentation: 新潟
  • Year and Date: 2011-07-29
• [Presentation] 血中診断マーカー獲得を目指した定量的プロテオミクス (2011)
  • Author(s): 小寺義男
  • Organizer: 日本プロテオーム学会2011大会・シンポジウム
  • Place of Presentation: 新潟
  • Year and Date: 2011-07-29
• [Presentation] 血清中の診断マーカータンパク質・ペプチドの探索と評価 (2010)
  • Author(s): 小寺義男
  • Organizer: 35回日本医用マススペクトル学会 (シンポジウム)
  • Place of Presentation: 名古屋
  • Year and Date: 2010-09-10
• [Presentation] 血清中の診断マーカータンパク質・ペプチドの探索と評価 (2010)
  • Author(s): 小寺義男, 野村文夫
  • Organizer: 第35回日本医用マススペクトル学会年会
  • Place of Presentation: 名古屋, 金城学院大学
  • Year and Date: 2010-09-10
• [Presentation] 血中ペプチドを対象とした各種がんの診断法開発を目指して (2010)
  • Author(s): 小寺義男
  • Organizer: 研究発表会「「がん」に立ち向かう神奈川のバイオベンチャーたち」 (招待講演)
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-24
• [Presentation] 血中ペプチドを対象とした各種がんの診断法開発を目指して (2010)
  • Author(s): 小寺義男
  • Organizer: 研究発表会「「がん」に立ち向かう神奈川のバイオベンチャーたち」
  • Place of Presentation: 横浜, 神奈川中小企業センタービル
  • Year and Date: 2010-06-24
• [Presentation] Comprehensive approach to develop diagnostic biomarker peptides in serum. –Establishment of discovery and validation methods targeting low-abundance peptides–
  • Author(s): Yoshio Kodera
  • Organizer: RCAI Seminar Series 2012
  • Place of Presentation: （独）理化学研究所・免疫アレルギー科学総合研究センター（RCAI)
  • Invited
• [Presentation] 血中疾患マーカーペプチド獲得を目指した包括的なアプローチ
  • Author(s): 小寺義男
  • Organizer: 東北大学医学部・最先端学術セミナー
  • Place of Presentation: 東北大学医学部
  • Invited
• [Book] 臨床プロテオミクス-バイオマーカー探索から個別化医療へ-, 第3 章 臨床試料の取り扱い「2.血液(血漿)および生体試液」 (2012)
  • Author(s): 小寺義男,朝長毅
  • Publisher: 金原出版株式会社
• [Book] 「血清・血漿バイオマーカー探索のための新しい前処理方法の開発」,実験医学別冊(創薬・タンパク質研究のためのプロテオミクス解析) (2010)
  • Author(s): 小寺義男、朝長毅
• [Patent(Industrial Property Rights)] METHOD FOR CONCENTRATION OF LOW-MOLECULAR-WEIGHT PROTEINS AND PEPTIDES IN BODY FLUID SAMPLE (2013)
  • Inventor(s): Kodera Y. Kawashima Y. Maeda T
  • Industrial Property Rights Holder: The Kitasato Institute
  • Acquisition Date: 2013-03-19
• [Patent(Industrial Property Rights)] 体液試料における、低分子量タンパク質及びペプチドの濃縮法 (2010)
  • Inventor(s): 小寺義男, 前田忠計, 川島祐介
  • Industrial Property Rights Holder: 学校法人北里研究所
  • Acquisition Date: 2010-08-20
• [Patent(Industrial Property Rights)] 体液試料における、低分子量タンパク質及びペプチドの濃縮法 (2010)
  • Inventor(s): 小寺義男, 前田忠計, 川島祐介
  • Industrial Property Rights Holder: 学校法人北里研究所
  • Acquisition Date: 2010-08-20