| Project/Area Number |
22390141
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | University of Yamanashi |
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Principal Investigator |
SHOJO Hideki 山梨大学, 大学院・医学工学総合研究部, 准教授 (60284626)
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| Co-Investigator(Renkei-kenkyūsha) |
ADACHI Noboru 山梨大学, 大学院・医学工学総合研究部, 教授 (60282125)
MABUCHI Tadashi 山梨大学, 大学院・医学工学総合研究部, 助教 (80150308)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
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| Keywords | 脳虚血 / 脳虚血モデル神経細胞 / 3次元画像解析 / ミトコンドリア / 虚血モデル神経細胞 |
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| Research Abstract |
In this study, we analyzed the neuropathological changes in cerebrovascular diseases and focused on the biomarker dynamics in order to understand a proper cause of death. Under ischemic conditions, we observed retrograde neurodegeneration, dendritic atrophy, and somatic swelling. Neuronal viability and respiration activity were significantly decreased in a dependent manner. These observations indicate that hypoxic conditions such as in ischemia, exacerbates the brain activity and the organelle function, especially in the mitochondria in which the morphology changed in the altered fixative conditions. These results demonstrate that observing the mitochondrial morphological changes in relation to the cause of death (e.g., sub- and acute death) would be a useful forensic diagnosis biomarker.
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