Forensic development of neuropathologicalanalyses for cerebrovascular diseases using ischemia model neuron
Project/Area Number |
22390141
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Legal medicine
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Research Institution | University of Yamanashi |
Principal Investigator |
SHOJO Hideki 山梨大学, 大学院・医学工学総合研究部, 准教授 (60284626)
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Co-Investigator(Renkei-kenkyūsha) |
ADACHI Noboru 山梨大学, 大学院・医学工学総合研究部, 教授 (60282125)
MABUCHI Tadashi 山梨大学, 大学院・医学工学総合研究部, 助教 (80150308)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
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Keywords | 脳虚血 / 脳虚血モデル神経細胞 / 3次元画像解析 / ミトコンドリア / 虚血モデル神経細胞 |
Research Abstract |
In this study, we analyzed the neuropathological changes in cerebrovascular diseases and focused on the biomarker dynamics in order to understand a proper cause of death. Under ischemic conditions, we observed retrograde neurodegeneration, dendritic atrophy, and somatic swelling. Neuronal viability and respiration activity were significantly decreased in a dependent manner. These observations indicate that hypoxic conditions such as in ischemia, exacerbates the brain activity and the organelle function, especially in the mitochondria in which the morphology changed in the altered fixative conditions. These results demonstrate that observing the mitochondrial morphological changes in relation to the cause of death (e.g., sub- and acute death) would be a useful forensic diagnosis biomarker.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Acute treatment with herbal extracts provides neuroprotective benefits in in vitro and in vivo stroke models, characterized by reduced ischemic cell death and maintenance of motor and neurological functions2010
Author(s)
Kaneko Y, Eve DJ, Yu SJ, Shojo H, Bae EC, Park D-H, Roschek B, Alberte RS, Sanberg PR, Sanberg CD, Bickford PC, Borlongan CV
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Journal Title
Cell Med
Volume: 1
Pages: 137-142
Related Report
Peer Reviewed
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[Presentation] Acute treatment of herbal extracts reduces ischemic cell death and improves motor and neurological functions in stroke models.2010
Author(s)
Borlongan CV, Yu S, KanekoY, Shojo H, Bae E, Park DH, Eve DJ, Roschek, BJ, Alberte RS, Sanberg PR, Sanberg CD, Bickford PC
Organizer
17thAnnual Conference of the American Society for Neural Therapy & Repair, Cell Transplant
Place of Presentation
Florida. U.S.A.
Related Report
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