| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Research Abstract |
In the present study, we tried to determine whether intratracheal administration of dry powdered low-molecular-weight chitosan (LMWC)/siRNA complexes suppressed gene expression in murine lung. Dry powdered LMWC/siRNA targeting green fluorescent protein (GFP-siRNA) and Lewis lung carcinoma cells stably expressing GFP (LLC-GFP) were prepared. Dry powdered LMWC/GFP-siRNA complex was intratracheally administered to GFP transgenic mice and the C57BL/6 mice carrying lung metastatic tumors consisting of LLC-GFP cells. The fluorescence intensity in the lung tissue sections was analyzed with a fluorescence microscope. Intratracheal administration of dry powdered LMWC/GFP-siRNA complex was found to suppress the fluorescence level of bronchi, bronchiole, alveoli, and metastatic lung tumors consisting of LLC-GFP cells. The results of the present study suggest that LMWC is an effective carrier for siRNA delivery to the lung, and dry powdered LMWC/siRNA complexes maybecome a promising tool to knock-down a specific gene expression in various lung diseases.
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