Trial to suppress gene expression in airway by intratracheal administration of dry-powdered siRNA
| Project/Area Number |
22390165
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hiroshima University |
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Principal Investigator |
HATTORI Noboru 広島大学, 大学院・医歯薬保健学研究院, 准教授 (00283169)
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| Co-Investigator(Renkei-kenkyūsha) |
OKAMOTO Hirokazu 名城大学, 薬学部, 教授 (00308941)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | RNA干渉 / GFP / 吸入療法 / ＲＮＡ干渉 / ＧＦＰ |
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| Research Abstract |
In the present study, we tried to determine whether intratracheal administration of dry powdered low-molecular-weight chitosan (LMWC)/siRNA complexes suppressed gene expression in murine lung. Dry powdered LMWC/siRNA targeting green fluorescent protein (GFP-siRNA) and Lewis lung carcinoma cells stably expressing GFP (LLC-GFP) were prepared. Dry powdered LMWC/GFP-siRNA complex was intratracheally administered to GFP transgenic mice and the C57BL/6 mice carrying lung metastatic tumors consisting of LLC-GFP cells. The fluorescence intensity in the lung tissue sections was analyzed with a fluorescence microscope. Intratracheal administration of dry powdered LMWC/GFP-siRNA complex was found to suppress the fluorescence level of bronchi, bronchiole, alveoli, and metastatic lung tumors consisting of LLC-GFP cells. The results of the present study suggest that LMWC is an effective carrier for siRNA delivery to the lung, and dry powdered LMWC/siRNA complexes maybecome a promising tool to knock-down a specific gene expression in various lung diseases.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Intra-airway administration of small interfering RNA targeting plasminogen activator inhibitor-1 attenuates allergic asthma in mice.2011
Author(s)
Miyamoto S, Hattori N, Senoo T, Onari Y, Iwamoto H, Kanehara M, Ishikawa N, Fujitaka K, Haruta Y, Murai H, Yokoyama A, Kohno N.
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Journal Title
Am J Physiol Lung Cell Mol Physiol
Volume: 301
Related Report
Peer Reviewed
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