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Analysis of WNK-NCC signaling cascade, a novel blood pressure controlling system, in the kidney

Research Project

Project/Area Number 22390168
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionTokyo Medical and Dental University

Principal Investigator

RAI TATEMITSU  東京医科歯科大学, 医学部附属病院, 准教授 (80334431)

Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywords偽性低アルドステロン血症II型 / KLHL3 / Cullin3 / ユビキチン / SPAK / Na-K-Cl共輸送体 / OSR1 / angiotensin II / 偽性低アルドステロン症II型 / Cullin 3 / Na-K-Cl 共輸送体 / WNKキナーゼ / Na-Cl共輸送体(NCC) / 遺伝子改変マウス / 高血圧 / 遺伝性高血圧
Research Abstract

The purpose of this study is to further characterize the WNK-NCC signal transduction system and to propose a novel strategy for treatment of hypertension.
Transgenic mice overexpressing WNK4 showed PHAII phenotypes, indicating that wild-type WNK4 protein was a positive regulator of NCC. KLHL3 interacted with Cullin3 and WNK4, induced WNK4 ubiquitination, and reduced the WNK4 protein level. PHAII-causing mutations in either protein reduced the ubiquitination of WNK4, resulting in an increased level of WNK4 protein. Thus, we found that WNK4 was a target for KLHL3-mediated ubiquitination, and impaired ubiquitination of WNK4 was the common mechanism of human hereditary hypertension.
As a result of screening chemical compounds that disrupted WNK/SPAK binding, we discovered three novel compounds that could be promising seeds of new types of antihypertensive drugs.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (54 results)

All 2014 2013 2012 2011 2010

All Journal Article (25 results) (of which Peer Reviewed: 25 results) Presentation (29 results)

  • [Journal Article] Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice2014

    • Author(s)
      Susa K, Sohara E, Rai T, Zeniya M, Mori Y, Mori T, Chiga M, Nomura N, Nishida H, Takahashi D, Isobe K, Inoue Y, Takeishi K, Takeda N, Sasaki S, Uchida S
    • Journal Title

      Hum Mol Genet

      Volume: (Epub ahead of print)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] WNK4 is the major WNK kinase positively regulating NCC in the mouse kidney2014

    • Author(s)
      Takahashi D, Mori T, Nomura N, Khan MZ, Araki Y, Zeniya M, Sohara E, Rai T, Sasaki S, Uchida S
    • Journal Title

      Biosci Rep

      Volume: (Epub ahead of print)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of with-no-lysine kinase signaling by Kelch-like proteins2014

    • Author(s)
      Uchida S, Sohara E, Rai T, Sasaki S
    • Journal Title

      Biol Cell

      Volume: 106(2) Issue: 2 Pages: 45-56

    • DOI

      10.1111/boc.201300069

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of enzyme-linked immunosorbent assays for urinary thiazide-sensitive Na-Cl cotransporter (NCC) measurement2013

    • Author(s)
      Isobe K, Mori T, Asano T, Kawaguchi H, Nonoyama S, Kumagai N, Kamada F, Morimoto T, Hayashi M, Sohara E, Rai T, Sasaki S, Uchida S
    • Journal Title

      Am J Physiol Renal Physiol

      Volume: 305(9) Issue: 9 Pages: F1374-F1381

    • DOI

      10.1152/ajprenal.00208.2013

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dietary salt intake regulates WNK3-SPAK-NKCC1 phosphorylation cascade in mouse aorta through angiotensin II2013

    • Author(s)
      Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S
    • Journal Title

      Hypertension

      Volume: 62(5) Issue: 5 Pages: 872-8

    • DOI

      10.1161/hypertensionaha.113.01543

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemical library screening for WNK signalling inhibitors using fluorescence correlation spectroscopy.2013

    • Author(s)
      Mori, T.; Kikuchi, E.; Watanabe, Y.; Fujii, S.; Ishigami-Yuasa, M.; Kagechika, H.; Sohara, E.; Rai, T.; Sasaki, S.; Uchida, S.
    • Journal Title

      Biochem. J.

      Volume: 455 Issue: 3 Pages: 339-345

    • DOI

      10.1042/bj20130597

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decrease of WNK4 ubiquitination by disease-causing mutations of KLHL3 through different molecular mechanisms2013

    • Author(s)
      Mori Y, Wakabayashi M, Mori T, Araki Y, Sohara E, Rai T, Sasaki S, Uchida S
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 439(1) Issue: 1 Pages: 30-4

    • DOI

      10.1016/j.bbrc.2013.08.035

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] KLHL2 interacts with and ubiquitinates WNK kinases2013

    • Author(s)
      Takahashi D, Mori T, Wakabayashi M, Mori Y, Susa K, Zeniya M, Sohara E, Rai T, Sasaki S, Uchida S
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 437(3) Issue: 3 Pages: 457-62

    • DOI

      10.1016/j.bbrc.2013.06.104

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension.2013

    • Author(s)
      Wakabayashi M
    • Journal Title

      Cell Rep.

      Volume: 28 Issue: 3 Pages: 858-868

    • DOI

      10.1016/j.celrep.2013.02.024

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCC co-transporters in vivo2012

    • Author(s)
      Oi K
    • Journal Title

      Biology Open

      Volume: Vol.1 Issue: 2 Pages: 120-127

    • DOI

      10.1242/bio.2011048

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] WNK-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone.2012

    • Author(s)
      Susa K
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 427 Issue: 4 Pages: 743-747

    • DOI

      10.1016/j.bbrc.2012.09.130

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Does a β2-adrenergic receptor-WNK4-Na-Cl co-transporter signal cascade exist in the in vivo kidney?2012

    • Author(s)
      Uchida S
    • Journal Title

      Nat. Med.

      Volume: 18 Issue: 9 Pages: 1324-1325

    • DOI

      10.1038/nm.2809

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylinositol 3-kinase/Akt signaling pathway activates the WNK - OSR1/SPAK - NCC phosphorylation cascade in hyperinsulinemic db/db Mice2012

    • Author(s)
      Nishida H, Sohara E, Nomura N, Chiga M, Alessi DR, Rai T, Sasaki S, Uchida S
    • Journal Title

      Hypertension

      Volume: 60(4) Pages: 981-90

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination.2012

    • Author(s)
      Hossain Khan MZ
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 425 Issue: 2 Pages: 456-461

    • DOI

      10.1016/j.bbrc.2012.07.124

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of heterozygous deletion of WNK1 on the WNK-OSR1 / SPAK-NCC / NKCC1 / NKCC2 signal cascade in the kidney and blood vessels2012

    • Author(s)
      Susa K, Kita S, Iwamoto T, Yang SS, Lin SH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR, Uchida S
    • Journal Title

      Clin Exp Nephrol

      Volume: 16(4) Pages: 530-8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels.2012

    • Author(s)
      Susa K
    • Journal Title

      Clin Exp Nephrol

      Volume: 16(4) Pages: 530-8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylinositol 3-kinase/Akt signaling pathway activates the WNK-OSR1/SPAK-NCC phosphorylation cascade in hyperinsulinemic db/db mice.2012

    • Author(s)
      Nishida H
    • Journal Title

      Hypertension

      Volume: 60 Issue: 4 Pages: 981-990

    • DOI

      10.1161/hypertensionaha.112.201509

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney2011

    • Author(s)
      Sohara E
    • Journal Title

      PLoS One

      Volume: Vol.6 Issue: 8 Pages: e24277-e24277

    • DOI

      10.1371/journal.pone.0024277

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypes of pseudohypoaldosteronism type II caused by the WNK4 D561A missense mutation are dependent on the WNK-OSR1/SPAK kinase cascade2011

    • Author(s)
      Chiga M
    • Journal Title

      J. Cell Sci.

      Volume: Vol.124 Issue: 9 Pages: 1391-5

    • DOI

      10.1242/jcs.084111

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of WNK1 kinase by extracellular potassium2011

    • Author(s)
      Naito S
    • Journal Title

      Clin. Exp. Nephrol.

      Volume: Vol.15 Issue: 2 Pages: 195-202

    • DOI

      10.1007/s10157-010-0378-9

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms for hypercalciuria in pseudohypoaldosteronism type II-causing WNK4 knock-in mice2010

    • Author(s)
      Yang SS, Hsu YJ, Chiga M, Rai T, Sasaki S, Uchida S, Lin SH
    • Journal Title

      Endocrinology

      Volume: 151(4) Issue: 4 Pages: 1829-36

    • DOI

      10.1210/en.2009-0951

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of angiotensin II on the WNK-OSR1/SPAK-NCC phosphorylation cascade in cultured mpkDCT cells and in vivo mouse kidney2010

    • Author(s)
      Talati G, Ohta A, Rai T, Sohara E, Naito S, Vandewalle A, Sasaki S, Uchida S
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 393(4) Issue: 4 Pages: 844-8

    • DOI

      10.1016/j.bbrc.2010.02.096

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of angiotensin II on the WNK-OSR1/SPAK-NCC phosphorylation cascade in cultured mpkDCT cells and in vivo mouse kidney.2010

    • Author(s)
      Talati G
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 393(4) Pages: 844-848

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms for hypercalciuria in pseudohypoaldosteronism type II-causing WNK4 knock-in mice.2010

    • Author(s)
      Yang SS
    • Journal Title

      Endocrinology

      Volume: 151(4) Pages: 1829-1836

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcium and calcimimetics regulate paracellular Na+transport in thethin ascending limb of Henle's loop in mouse kidney.2010

    • Author(s)
      Sugawara N
    • Journal Title

      Pflugers Arch.

      Volume: 460(1) Pages: 197-205

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Clinical significance of urinary thiazide-sensitive Na-Cl cotransporter (NCC) measurement by newly developed enzyme-linked immunosorbent assays2013

    • Author(s)
      Isobe K, Mori T, Sohara E, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2013
    • Place of Presentation
      Atlanta, U.S.A.
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Low salt intake decreased transcription and protein level of KLHL3 in mouse kidney2013

    • Author(s)
      Susa K, Sohara E, Zeniya M, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2013
    • Place of Presentation
      Atlanta, U.S.A.
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Chemical library screening for WNK signaling inhibitors by using fluorescent correlation spectroscopy2013

    • Author(s)
      Mori T, Kikuchi E, Sohara E, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2013
    • Place of Presentation
      Atlanta, U.S.A.
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Impaired KHLH3-mediated ubiquitination of WNK4 activates OSR1 and SPAK kinases - NaCl cotransporter (NCC) signaling and causes hypertension2013

    • Author(s)
      Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2013
    • Place of Presentation
      Atlanta, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Analyses of KHLH3 mutants that cause Pseudohypoaldosteronism type II2013

    • Author(s)
      Mori Y, Wakabayashi M, Mori T, Araki Y, Sohara E, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2013
    • Place of Presentation
      Atlanta, U.S.A.
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] KHLH2 interacts with and ubiquitinates WNK4 kinases2013

    • Author(s)
      Takahashi D, Mori T, Wakabayashi M, Mori Y, Susa K, Zeniya M, Rai T, Sohara E, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2013
    • Place of Presentation
      Atlanta, U.S.A.
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] WNK3 regulates blood pressure through the regulation of vascular OSR1 / SPAK-NKCC1 phosphorylation cascade2013

    • Author(s)
      Zeniya M, Sohara E, Oi K, Chiga M, Susa K, Mori T, Takahashi D, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2013
    • Place of Presentation
      Atlanta, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Impaired KHLH3-mediated ubiquitination of WNK4 activates OSR1 and SPAK kinases-NaCl cotransporter (NCC) signaling and causes hypertension2013

    • Author(s)
      Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2013
    • Place of Presentation
      Atlanta, U.S.A.
    • Related Report
      2013 Annual Research Report
  • [Presentation] WNK3 regulates blood pressure through the regulation of vascular OSR1/SPAK-NKCC1 phosphorylation cascade2013

    • Author(s)
      Zeniya M, Sohara E, Oi K, Chiga M, Susa K, Mori T, Takahashi D, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2013
    • Place of Presentation
      Atlanta, U.S.A.
    • Related Report
      2013 Annual Research Report
  • [Presentation] Chemical library screening for direct SPAK inhibitors by a newly devoloped ELISA2012

    • Author(s)
      Kikuchi E, Mori T, Isobe K, Sohara E, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of sandwich enzyme linked immunosorbent assay for measurement of urinary total and phosphorylated Na-Cl cotransporter protein2012

    • Author(s)
      Isobe K, Sohara E, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Dietary salt intake and angiotensin II regulates WNK - SPAK - NKCC1 phosphorylation cascade in mouse aorta2012

    • Author(s)
      Zeniya M, Sohara E, Oi K, Chiga M, Susa K, Mori T, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination2012

    • Author(s)
      Kahn MZH, Sohara E, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Generation and analysis of WT-WNK4 transgenic mice reveal the physiological role of WNK42012

    • Author(s)
      Mori T, Sohara E, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] WNK4 - OSR1/SPAK - NCC signal cascade has circadian rhythm dependent on aldosterone2012

    • Author(s)
      Susa K, Sohara E, Isobe K, Chiga M, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Chemical Library Screening for Direct SPAK Inhibitors by a Newly Devoloped ELISA.2012

    • Author(s)
      Kikuchi E
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of Sandwich Enzyme Linked Immunosorbent Assay for Measurement of Urinary Total and Phosphorylated Na-Cl Cotransporter Protein.2012

    • Author(s)
      Isobe K
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Dietary Salt Intake and Angiotensin II Regulates WNK-SPAK-NKCC1 Phosphorylation Cascade in Mouse Aorta.2012

    • Author(s)
      Zeniya M
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Phosphorylation of Na-Cl Cotransporter by OSR1 and SPAK Kinases Regulates Its Ubiquitination.2012

    • Author(s)
      Kahn MZH
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Generation and Analysis of WT-WNK4 Transgenic Mice Reveal the Physiological Role of WNK4.2012

    • Author(s)
      Mori T
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] WNK4-OSR1/SPAK-NCC Signal Cascade Has Circadian Rhythm Dependent on Aldosterone.2012

    • Author(s)
      Susa S
    • Organizer
      American Society of Nephrology Kidney Week 2012
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Acute Insulin Stimulation Induces Phosphorylation of the Na-Cl Cotransporter in Cultured Distal mpkDCT Cells and Mouse Kidney2011

    • Author(s)
      Rai T
    • Organizer
      American Society of Nephrology Kidney Week 2011
    • Place of Presentation
      Philadelphia
    • Year and Date
      2011-11-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Increased Na-Cl cotransporter phosphorylation in hyperinsulinemic db/db mice is regulated by insulin/PI3K pathway2011

    • Author(s)
      Nishida H, Sohara E, Alessi D, Nomura N, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2011
    • Place of Presentation
      Philadelphia, U.S.A
    • Related Report
      2013 Final Research Report
  • [Presentation] Increased protein abundance of the mutant WNK4 may be a cause of the increased WNK4 kinase activity in the mouse model of pseudohypoaldosteronism type II(PHA II).2011

    • Author(s)
      Wakabayashi M, Naito S, Sohara E, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2011
    • Place of Presentation
      Philadelphia, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney2011

    • Author(s)
      Sohara E, Rai T, Yang SS, Ohta A, Lin SH, Vandewalle A, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2011
    • Place of Presentation
      Philadelphia, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of new systems to measure total and phosphorylated Na-Cl cotransporter (NCC) protein in human urine2011

    • Author(s)
      Isobe K, Sohara E, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2011
    • Place of Presentation
      Philadelphia, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] Urinary excretion of Na-Cl cotransporter in exosomes is increased by high salt diet as well as low salt diet2011

    • Author(s)
      Kahn MZH, Sohara E, Ohta A, Naito S, Chiga M, Rai T, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2011
    • Place of Presentation
      Philadelphia, U.S.A.
    • Related Report
      2013 Final Research Report
  • [Presentation] WNK - OSR1/SPAK - SLC12A phosphorylation cascade in the WNK1 (+/-) mice2011

    • Author(s)
      Susa K, Sohara E, Rai T, Kita S, Iwamoto T, Alessi D, Sasaki S, Uchida S
    • Organizer
      American Society of Nephrology Kidney Week 2011
    • Place of Presentation
      Philadelphia, U.S.A
    • Related Report
      2013 Final Research Report
  • [Presentation] 偽性低アルドステロン症II型モデルマウスにおけるNCCのリン酸化充進はOSR1とSPAKキナーゼの活性に依存する2010

    • Author(s)
      頼建光
    • Organizer
      第53回日本腎臓学会学術総会
    • Place of Presentation
      神戸
    • Year and Date
      2010-06-17
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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