| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
|
|---|
| Research Abstract |
TDP-43 is a RNA binding protein and associated with the pathogenesis of ALS. However, the physiological functions of TDP-43, especially related to the disease pathogenesis are not fully understood. In this study, we show that TDP-43 facilitates the production of a subset of pre-miRNAs by both interacting with the Drosha complex and binding directly to the relevant pri-miRNAs. Furthermore, cytoplasmic TDP-43, which interacts with the Dicer complex, promotes the processing of some of these pre-miRNAs. Finally, we show that involvement of TDP-43 in miRNA biogenesis is indispensable for neuronal outgrowth. These results support a previously uncharacterized role for TDP-43 in post-transcriptional regulation of miRNA expression.
|
