Functional molecular imaging studies by rapid EPR imaging method

• Project/Area Number: 22390236
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Sapporo Medical University
• Principal Investigator: FUJII Hirotada 札幌医科大学, 医療人育成センター, 教授 (70209013)
• Co-Investigator(Renkei-kenkyūsha): HIRATA Hiroshi 北海道大学, 大学院・情報科学研究科, 教授 (60250958) ; ITOH Kouichi 徳島文理大学, 薬学部, 教授 (30291149) ; NAGASAKI Yukio 筑波大学, 数理物質科学研究所, 教授 (90198309)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 電子常磁性共鳴 / イメージング / ESR / EPR / 酸化ストレス / 活性酸素 / 非侵襲 / 高速計測 / 生理機能 / MRI / 酸素

Research Abstract

Electron paramagnetic resonance (EPR) imaging using nitroxides is a powerful, noninvasive method for visualizing the redox status modulated by oxidative stress in vivo. However, to date, it has not always been possible to obtain three-dimensional (3D) images with a continuous wave (CW)-EPR imager in small rodents while using nitroxides. Due to improvements in imagers that enable rapid data-acquisition, the feasibility of 3D EPR imaging with good quality in mice was tested with nitroxides of different lipophilicities. The improved CW-EPR imager may be a useful noninvasive tool for assessing changes in the redox status of living subjects under oxidative stress.

Research Products

• [Journal Article] Novel ascorbic acid-resistive nitroxide in a lipid emulsion: An efficient brain imaging contrast agent for MRI of small rodents. (2013)
  • Author(s): Emoto CM, Yamada K, Yamato M, Fujii GH.
  • Journal Title: Neuroscience Letters.
  • Peer Reviewed
• [Journal Article] odified oxygen radical absorbance capacity assay that can be implemented at low temperatures: A pilot study. (2013)
  • Author(s): Kohri, S, Fujii H.
  • Journal Title: Food Sci. Technol Res Volume: 19 Pages: 269-276
  • Peer Reviewed
• [Journal Article] Noninvasive mapping of the redox status in septic mouse by in vivo electron paramagnetic resonance imaging. (2013)
  • Author(s): Fujii HG, Sato-Akaba H, Emoto MC, ItohK, Ishihara Y, Hirata H.
  • Journal Title: Magn Reson Imag Volume: 31 Pages: 130-138
  • Peer Reviewed
• [Journal Article] Noninvasive mapping of the redox status in septic mouse by in vivo electron paramagnetic resonance imaging. (2013)
  • Author(s): Hirotada Fujii
  • Journal Title: Magnetic Resonance Imaging Volume: 31 Issue: 1 Pages: 130-138
  • DOI: 10.1016/j.mri.2012.06.021
  • Peer Reviewed
• [Journal Article] Electron ParamagneticResonance-Based pH Mapping Using Spectral-Spatial Imaging of Sequentially Scanned Spectra. (2012)
  • Author(s): Koda S, Goodwin J, Khramtsov VV, Fujii H, Hirata H.
  • Journal Title: Anal Chem. Volume: 84 Pages: 3833-3837
  • Peer Reviewed
• [Journal Article] Oxygen Radical Absorbance Capacity (ORAC) of cyclodextrin-solubilized flavonoids,resveratrol and astaxanthin as measured with the ORAC-EPR method. (2012)
  • Author(s): Sueishi Y, Ishikawa M, Yoshioka D, Endoh N, Oowada S, Shimmei M, Fujii H, Kotake Y.
  • Journal Title: J Clin Biochem Natr Volume: 50 Pages: 127-132
  • NAID: 130004466680
  • Peer Reviewed
• [Journal Article] A novel ascorbic acid-resistant nitroxide in fat emulsion is an efficient brain imaging probe for in vivo EPR imaging of mouse. (2011)
  • Author(s): Emoto M, Mito F, Yamasaki T, Yamada K, Sato-Akaba H, Hirata H, Fujii H.
  • Journal Title: Free Radic Res. Volume: 45 Pages: 1325-1332
  • Peer Reviewed
• [Journal Article] Aged garlic extract scavenges superoxide radicals. (2011)
  • Author(s): Morihara N, Hayama M, Fujii H.
  • Journal Title: Plant Foods Hum Nutr. Volume: 66 Pages: 17-21
  • Peer Reviewed
• [Journal Article] Simultaneous molecular imaging based on electron paramagnetic resonance of 14Nand 15N-labelled nitroxyl radicals. (2011)
  • Author(s): Pawlak A, Ito R, Fujii H, Hirata H.
  • Journal Title: Chem Comm. Volume: 47 Pages: 3245-3247
  • Peer Reviewed
• [Journal Article] Mapping of Redox Status in a Brain-Disease Mouse Model by Three-Dimensional EPR Imaging. (2011)
  • Author(s): Fujii H, Sato-Akaba H, Kawanishi K, Hirata H.
  • Journal Title: Mag Reson Med. Volume: 65 Pages: 295-303
  • Peer Reviewed
• [Journal Article] A novel ascorbic acid-resistive nitroxide in fat emulsion is an effective (2011)
  • Author(s): Emoto, M., Fujii, H.
  • Journal Title: Free Radical Research Volume: 45 Issue: 11-12 Pages: 1325-1332
  • DOI: 10.3109/10715762.2011.618499
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Fujii H., et al.
  • Journal Title: Magnetic Resonance in Medicine Volume: 65 Pages: 295-303
  • Peer Reviewed
• [Presentation] Brain redox mapping in methamphetamine-treated mice using three-dimensional EPR imaging (2013)
  • Author(s): Hirotada Fujii, Miho Emoto, Mayumi Yamato, Ken-ichi Yamada
  • Organizer: ISMRM2013
  • Place of Presentation: Salt-lake city
  • Year and Date: 2013-04-25
• [Presentation] Redox map of mouse brain by three-dimensional EPR imaging with six-membered nitroxyl radicals (2013)
  • Author(s): Miho Emoto, Hideo Sato-Akaba, Hiroshi Hirata, Hirotada Fujii
  • Organizer: ISMRM2013
  • Place of Presentation: Salt-lake city
  • Year and Date: 2013-04-25
• [Presentation] Differences in distribution and reduction rate of BBB permeable nitroxides by in vivo EPR imaging method (2012)
  • Author(s): Miho Emoto, Hideo Sato-Akaba, Hiroshi Hirata, Hirotada Fujii
  • Organizer: SFRBM2012
  • Place of Presentation: San Antonio
  • Year and Date: 2012-11-20
• [Presentation] Novel ascorbic acid-resistive nitroxides in fat emulsion: An efficient brain imaging probe for MRI of small rodents (2012)
  • Author(s): Hirotada Fujii, Miho Emoto, Mayumi Yamato, Ken-ichi Yamada
  • Organizer: SFRBM2012
  • Place of Presentation: San Antonio
  • Year and Date: 2012-11-20
• [Presentation] EPR イメージングによる六員環ニトロキシドのマウス頭部における酸化還元状態の評価 (2012)
  • Author(s): 江本美穂、赤羽英夫、平田拓、藤井博匡
  • Organizer: SEST2012
  • Place of Presentation: 札幌市
  • Year and Date: 2012-11-01
• [Presentation] グルコース Tempo スピンプローブを用いたEPR イメージング (2012)
  • Author(s): 藤井博匡、佐藤慎吾、江本美穂、平田拓
  • Organizer: SEST2012
  • Place of Presentation: 札幌市
  • Year and Date: 2012-11-01
• [Presentation] 高速 EPR イメージングシステムによるマウス脳内レドックス状態の評価について (2011)
  • Author(s): 藤井博匡、江本美穂、赤羽英夫、平田拓
  • Organizer: SEST2011
  • Place of Presentation: 仙台市
  • Year and Date: 2011-11-17
• [Presentation] 新規アスコルビン酸耐性ニトロキシドによるマウス頭部の in vivo EPR イメージング (2011)
  • Author(s): 江本美穂、水戸文弥、山崎俊栄、山田健一、赤羽英夫、平田拓、藤井博匡
  • Organizer: SEST2011
  • Place of Presentation: 仙台市
  • Year and Date: 2011-11-17