| Project/Area Number |
22390244
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOYAMA Hiroyuki 東京大学, 医学部附属病院, 特任准教授 (10241994)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Tetsurou 東京大学, 医学部附属病院, 准教授 (70190791)
MIURA Yutaka 東京大学, 医学(系)研究科(研究院), 助教 (40557980)
木村 秀生 東京大学, 医学部附属病院, 助教 (60327070)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | 人工臓器学 / 再生医学 / 血管新生 / 再生組織 / 栄養血管 / スフェロイド / 膵島移植 |
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| Research Abstract |
Three-dimensional (3-D) regenerative tissue with a certain bulk cannot maintain whole viability in vivo without sufficient blood perfusion. In the present study, we first established a technology to construct neovascular network around small tissue elements (spheroids) by combining with “angiogenic gel”. Angiogenic gel has a unique function to rapidly induce neovascular growth from recipient bed into the inside of the material. Subsequently, we created a new-type angiogenic gel which could also function as a scaffold to support 3-D accumulation of spheroids. A bulky regenerative tissue consisting of accumulated spheroids and the new-type gel was transplanted in vivo to attempt a construction of vascular network covering whole part of the tissue, though viability of spheroids declined in the central part of the tissue. To preserve the viability of all spheroids in the regenerative tissue, we developed anin-gel perfusion system, and a perfusion trial using culture medium showed effectiveness of the system.
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