Clinical significance of serum exosomal micro RNA in human esophageal squamous cellcarcinoma

• Project/Area Number: 22390247
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Kumamoto University
• Principal Investigator: BABA Hideo 熊本大学, 大学院・生命科学研究部, 教授 (20240905)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Naoko 熊本大学, 医学部附属病院, 非常勤診療医師 (20452899) ; WATANABE Masayuki 熊本大学, 大学院・生命科学研究部, 准教授 (80254639) ; 蒲原 英伸 熊本大学, 大学院生命科学研究部, 准教授 (90398222)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 食道癌 / miR / miRNA / microRNA / Exosome / Cancer / 分子生物学 / 実験外科学

Research Abstract

Exosomes are 40-nm to 100-nm membrane vesicles that are secreted by various cells, andthey play a major role in cell-cell communication. The expression levels of exosomalmiR-21 were significantly higher in patients with ESCC than those with benign diseases.Exosomal miR-21 was positively correlated with tumor progression and aggressiveness,suggesting that it may be a useful target for cancer therapy.

Research Products

• [Journal Article] Clinical impact of serum exosomalmicroRNA-21 in human esophagealsquamous cell carcinoma, as a clinicalbiomarker (2013)
  • Author(s): Tanaka Y,Kammohara H,KinoshitaK,Kurashige J,Ishimoto T, Iwatsuki M,Watanabe M, Baba H
  • Journal Title: Cancer Volume: 119 Issue: 6 Pages: 1159-1167
  • DOI: 10.1002/cncr.27895
  • Peer Reviewed
• [Journal Article] Overexpressionitin ligase FBXW7in esopha of microRNA-223 regulatesthe ubiqu geal squamous cellcarcinoma (2012)
  • Author(s): Kurashige J, Watanabe M, Iwatsuki M,Kinoshita K, Saito S,Hiyoshi Y,Kamohara H, Baba H, Mimori K
  • Journal Title: Br J Cancer Volume: 106 Issue: 1 Pages: 182-188
  • DOI: 10.1038/bjc.2011.509
  • Peer Reviewed
• [Journal Article] Micro RNA- 200b regulates cellproliferation, invasion and migrationby directly targeting ZEB2 in gastriccarcinoma (2012)
  • Author(s): Kurashige J, Kamohara H, Watanabe M,Hiyoshi Y, Iwatsuki M, Tanaka Y,Kinoshita K, Saito S, Baba Y, Baba H
  • Journal Title: Ann Surg Oncol 19 Suppl Volume: 3 Issue: S3 Pages: 656-64
  • DOI: 10.1245/s10434-012-2217-6
  • Peer Reviewed
• [Journal Article] Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma (2012)
  • Author(s): Kurashige J
  • Journal Title: Journal of Surgical Oncology Volume: (印刷中) Issue: 2 Pages: 188-92
  • DOI: 10.1002/jso.23064
  • Peer Reviewed
• [Journal Article] Carcinogenesis of intraductal papillary mucinous neoplasm of the pancreas : loss of microrna-101 promotes overexpression of histone methyltransferase EZH2 (2012)
  • Author(s): Nakahara O
  • Journal Title: Annals of Surgical Oncology Volume: (印刷中) Issue: S3 Pages: 565-71
  • DOI: 10.1245/s10434-011-2068-6
  • Peer Reviewed
• [Journal Article] Induction chemotherapy with docetaxel/cisplatin/5-fluorouracil for patients with node-positive esophageal cancer. (2010)
  • Author(s): Watanabe M, Nagai Y, Kinoshita K, Saito S, Kurashige J, Karashima R, Hirashima K, Sato N, Imamura Y, Hiyoshi Y, Baba Y, Iwagami S, Miyamoto Y, Iwatsuki M, Hayashi N, Baba H
  • Journal Title: Digestion Volume: 83 Pages: 146-152
  • Peer Reviewed
• [Journal Article] Irinotecan plus S-1 (IRIS) versus fluorouracil and folinic acid plus irinotecan (FOLFIRI) as second-line chemotherapy for metastatic colorectal cancer : a randomised phase 2/3 non-inferiority study (FIRIS study). (2010)
  • Author(s): Muro K, Boku N, Shimada Y, Tsuji A, Sameshima S, Baba H, Satoh T, Denda T, Ina K, Nishina T, Yamaguchi K, Takiguchi H, Esaki T, Tokunaga S, Kuwano H, Komatsu Y, Watanabe M, Hyoso I, Morita S, Sugihara K
  • Journal Title: Lancet Oncol Volume: 11 Pages: 853-860
  • Peer Reviewed
• [Journal Article] Phosphorylated mTOR expression is associated with poor prognosis for patients with esophageal squamous cell carcinoma. (2010)
  • Author(s): Hirashima K, Baba Y, Watanabe M, Karashima R, Sato N, Imamura Y, Hiyoshi Y, Nagai Y, Hayashi N, Iyama K, Baba H
  • Journal Title: Ann Surg Oncol Volume: 17 Pages: 2486-2493
  • Peer Reviewed
• [Journal Article] Adenoviral oncolytic suicide gene therapy for a peritoneal dissemination model of gastric cancer in mice. (2010)
  • Author(s): Imamura Y, Ishikawa S, Sato N, Karashima R, Hiyoshi Y, Nagai Y, Koga Y, Hayashi N, Watanabe M, Yamada G Baba H
  • Journal Title: Ann Surg Oncol Volume: 17 Pages: 643-652
  • Peer Reviewed
• [Journal Article] Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma: Detection of CEA-mRNA in the 3-field lymph nodes. (2010)
  • Author(s): Imamura Y, Hayashi N, Sato N, Kinoshita K, Kurashige J, Saito S, Hirashima K, Karashima R, Hiyoshi Y, Nagai Y, Watanabe M, Baba H
  • Journal Title: J Surg Oncol Volume: 4 Pages: 509-515
  • Peer Reviewed
• [Presentation] 食道癌における血清オキソノーム中の microRNA 発現の検討～biomarler としての可能性～ (2012)
  • Author(s): 田中洋平、蒲原英伸、澤山 浩、志垣博信、岡部弘尚、渡邊雅之、馬場秀夫
  • Organizer: 第50回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2012-10-26
• [Presentation] 食道癌における血清エキソソームが内包する microRNA～バイオマーカーとしての検討～ (2012)
  • Author(s): 田中洋平、蒲原英伸、蔵重淳二、渡邊雅之、馬場秀夫
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(北海道)
  • Year and Date: 2012-09-21
• [Presentation] 食道癌患者と非癌患者における血清エキソソーム中の microRNA 発現の比較検討 (2012)
  • Author(s): 田中洋平、蒲原英伸、澤山 浩、志垣博信、美馬浩介、蔵重淳二、岡部弘尚、渡邊雅之、馬場秀夫
  • Organizer: 第67回日本消化器外科学会
  • Place of Presentation: 富山県民会館 (富山県)
  • Year and Date: 2012-07-20
• [Presentation] 食道癌患者と非癌患者の血清中に存在するエキソソームの microRNA 発現の比較検討 (2012)
  • Author(s): 田中洋平、渡邊雅之、蒲原英伸、志垣博信、澤山浩、藏重淳二、木下浩一、齋藤誠也、岡部弘尚、長井洋平、井田智、石本崇胤、岩槻政晃、馬場秀夫
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2012-04-13
• [Presentation] The expression of exosomal microRNAin serum of patients with esophagealsquamous cell carcinoma (2012)
  • Author(s): Tanaka Y,Watanabe M, Kamohara H,Kurashige J, Mima K, Okabe H, Baba H
  • Organizer: AACR Annual Meeting2012
  • Place of Presentation: McCormick Place West (アメリカ・シカゴ)
  • Year and Date: 2012-04-03
• [Presentation] The expression of exosomal microRNA in serum of patients with esophageal squamous cell carcinoma (2012)
  • Author(s): Tanaka Y
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: McCormick Place West（アメリカ・シカゴ）
• [Presentation] 食道癌患者と非癌患者における血清エキソソーム中のmicroRNA発現の比較検討 (2012)
  • Author(s): 田中洋平
  • Organizer: 第67回日本消化器外科学会
  • Place of Presentation: 富山県民会館
• [Presentation] 食道癌における血清オキソノーム中のmicroRNA発現の検討～biomarlerとしての可能性～ (2012)
  • Author(s): 田中洋平
  • Organizer: 第50回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 食道癌患者と非癌患者の血清中に存在するエキソソームのmicroRNA発現の比較検討 (2012)
  • Author(s): 田中洋平
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 幕張メッセ国際会議場 千葉県
• [Presentation] 切除不能膵癌におけるバイオマーカーとしてのmicroRNA-21発現の意義 (2011)
  • Author(s): 中原修
  • Organizer: JDDW2011
  • Place of Presentation: 福岡マリンメッセ福岡
  • Year and Date: 2011-10-22
• [Presentation] 食道癌の発育進展におけるmTORの関与とmiRNAによる制御 (2011)
  • Author(s): 木下浩一
  • Organizer: 第65回日本食道学会学術集会
  • Place of Presentation: (震災の為、紙上発表)
  • Year and Date: 2011-09-26
• [Presentation] Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma (2011)
  • Author(s): Kurashige J
  • Organizer: AACR102nd Annual Meeting 2011
  • Place of Presentation: Orlando USA
  • Year and Date: 2011-04-02
• [Presentation] MicroRNA-29c expression as a new prognostic marker for esophageal squamous cell carcinoma (2011)
  • Author(s): Kinoshita K
  • Organizer: AACR102nd Annual Meeting 2011
  • Place of Presentation: Orlando USA
  • Year and Date: 2011-04-02
• [Presentation] Carcinogenesis of intraductal papillary mucinous neoplasm of the pancreas : Loss of microRNA-101 promotes overexpression of histone methyltransferase EZH2 (2011)
  • Author(s): Nakahara O
  • Organizer: AACR102nd Annual Meeting 2011
  • Place of Presentation: Orlando USA
  • Year and Date: 2011-04-02
• [Presentation] The Identificationof novel micro RNAs and Genes in bone marrow regulating gastric cancer metastasis (2011)
  • Author(s): Iwatsuki M
  • Organizer: the 8th International Symposium on Minimal Residual Cancer
  • Place of Presentation: 大阪グランキューブ大阪
• [Presentation] 胃癌転移を制御する骨髄中新規 microRNA、 遺伝子の同定 (2010)
  • Author(s): 岩槻政晃、三森功士、渡邊雅之、深川剛生、森 正樹、馬場秀夫
  • Organizer: JDDW2010
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2010-10-15
• [Presentation] 腫瘍マーカーとしてのmicroRNAの可能性 (2010)
  • Author(s): 蔵重淳二、渡邊雅之、岩槻政晃、齋藤誠哉、木下浩一、佐藤伸隆、平島浩太郎、辛島龍一、今村裕、日吉幸晴、長井洋平、尾崎宣之、宮本裕士、林尚子、馬場秀夫
  • Organizer: JDDW2010第8回日本消化器外科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-10-15
• [Presentation] 胃癌転移を制御する骨髄中新規microRNA、遺伝子の同定 (2010)
  • Author(s): 岩槻政晃、三森功士、渡邊雅之、深川剛生、森 正樹、馬場秀夫
  • Organizer: JDDW2010第9回日本消化器外科学会大会肝臓学会・消化器病学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-10-15
• [Presentation] 食道癌におけるmicroRNA発現パターンと予後との相関 (2010)
  • Author(s): 木下浩一、蒲原英伸、岩槻政晃、藏重淳二、斎藤誠哉、長井洋平、池田貯、林尚子、渡邊雅之、馬場秀夫
  • Organizer: 第65回日本消化器外科学会総会
  • Place of Presentation: 下関グランドホテル
  • Year and Date: 2010-07-14
• [Presentation] microRNAによる食道癌抗癌剤効果予測の可能性 (2010)
  • Author(s): 蔵重淳二、渡邊雅之、木下浩一、齋藤誠也、日吉幸晴、岩槻政晃、蒲原英伸、池田貯、林尚子、馬場秀夫
  • Organizer: 第65回日本消化器外科学会総会
  • Place of Presentation: 下関グランドホテル
  • Year and Date: 2010-07-14
• [Presentation] MicroRNA-21 and -26a are novel markers to predict the sensitivity to chemotherapy in esophageal squamous cell carcinoma. (2010)
  • Author(s): Kurashige J, Iwatsuki M, Hiyoshi Y, Kamohara H, Kinoshita K, Saito S, Sato N, Karashima R, Hirashima K, Watanabe M, Baba H
  • Organizer: AACR
  • Place of Presentation: Walter E Washington Convention Center, USA
• [Presentation] Micro RNA-101 promotes apoptosis and suppresses proliferation in esophageal squamous cell carcinoma by repressing the polycomb group protein. (2010)
  • Author(s): Hiyoshi Y, Kamohara H, Kurashige J, Kinoshita K, Hayashi N, Watanabe M, Baba H.
  • Organizer: AACR
  • Place of Presentation: Walter E Washington Convention Center, USA
• [Presentation] microRNAによる食道扁平上皮癌抗癌剤効果予測 (2010)
  • Author(s): 蔵重淳二、渡邊雅之、岩槻政晃、木下浩一、齋藤誠也、日吉幸晴、蒲原英伸、林尚子、馬場秀夫
  • Organizer: 第64回日本食道学会学術集会
  • Place of Presentation: 石橋美術館
• [Presentation] Micrro RNA-101 promotes apotosis and suppresses proliferation in esophageal squamous cell carcinoma by repressing the polycomb group orpte in EZH2 (2010)
  • Author(s): Kurashige J, Hiyoshi Y, Kamohara H, Iwatsuki M, Kinoshita K, Saito S, Karashima R, Hirashima K, Hayashi N, Watanabe M, Baba H
  • Organizer: 7th International symposium on cancer research and therapy (ISCRT)
  • Place of Presentation: 東京会館