Functional analysis of Sox11 and Plagl1 in glioma stem cells.

• Project/Area Number: 22390278
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Hokkaido University (2012); Ehime University (2010-2011)
• Principal Investigator: KONDO Toru 北海道大学, 遺伝子病制御研究所, 教授 (30270573)
• Co-Investigator(Renkei-kenkyūsha): OHNISHI Takanori 愛媛大学, 大学院・医学系研究科, 教授 (70233210) ; KOMATSU Kenshi 京都大学, 放射線生物研究センター, 教授 (80124577)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 脳腫瘍学 / グリオーマ幹細胞 / Sox11 / Plagl1 / エピジェネティクス / Plag11 / Soxll

Research Abstract

We have examined about the expression of Sox11 and Plagl1 and their functions in glioma stem cells (GSC) and neural stem cells (NSC) and found the following results. (1) Expression of Sox11 in GSC is not regulated by either NeuroD or Neurogenin, both of which induce Sox11 expression in neural differentiation. We have identified the DNA domain (200bp) that regulates Sox11 expression in GSC. (2) Plagl1 is involved in both the maintenance of stemness in NSC and their DNA repair. (3) We have established efficient methods for human GSC preparation and their maintenance and examined their expression profiles.

Research Products

• [Journal Article] 癌幹細胞を標的とし た治療のこれからーグリオーマ幹細胞をモ デルとして (2013)
  • Author(s): 近藤亨
  • Journal Title: Frontiers in Gastroenterology Volume: 18 Pages: 68-73
• [Journal Article] 癌幹細胞を標的とした治療のこれからーグリオーマ幹細胞をモデルとして (2013)
  • Author(s): 近藤 亨
  • Journal Title: Frontiers in Gastroenterology Volume: 18
• [Journal Article] 脳腫瘍の癌幹細胞治療戦略 (2011)
  • Author(s): 近藤亨
  • Journal Title: 医薬ジャーナル Volume: 47 Pages: 103-108
• [Journal Article] 脳腫瘍のCSC (2011)
  • Author(s): 近藤亨
  • Journal Title: BIO Clinica Volume: 26 Pages: 39-43
• [Journal Article] グリオーマ (2011)
  • Author(s): 近藤亨
  • Journal Title: 臨床検査 Volume: 55 Pages: 477-482
• [Journal Article] Tumorigenesis of glioma-initiating cells: Role of Sox11 (2011)
  • Author(s): Kondo, T.
  • Journal Title: Stem Cells and Cancer Stem Cells Pages: 93-98
  • URL: http://link.springer.com/book/10.1007/978-94-007-1709-1/page/1
  • Peer Reviewed
• [Journal Article] Combination of a Ptgs2 inhibitor and an EGFR signaling inhibitor prevents tumorigenesis of oligodendrocyte -lineage derived glioma-initiating cells (2011)
  • Author(s): Hide, T., Takezaki, T., Nakatani, Y., Nakamura, H., Kuratsu, J., & Kondo, T
  • Journal Title: Stem Cells Volume: 29 Issue: 4 Pages: 590-599
  • DOI: 10.1002/stem.618
  • Peer Reviewed
• [Journal Article] Cytotixic T lymphocytes efficiently recognize human colon cancer stem-like cells (2011)
  • Author(s): Inoda, S., Hirohashi, Y., Torigoe, T., Takahashi, A., Morita, R., Nakatsugawa, M., Nishizawa, S., Tamura, Y., Tsuruma, T., Terui, T., Ishitani, K., Hirata, K. and Sato, N.
  • Journal Title: Am. J. Pathol. Volume: 178 Issue: 4 Pages: 1805-1813
  • DOI: 10.1016/j.ajpath.2011.01.004
  • Peer Reviewed
• [Journal Article] Essential role of the Hedgehog signaling pathway in human glioma-initiating cells (2011)
  • Author(s): Takezaki, T., Hide, T., Takanaga, H., Nakamura, H., Kuratsu, J., & Kondo, T
  • Journal Title: Cancer Science Volume: 102 Issue: 7 Pages: 1306-1312
  • DOI: 10.1111/j.1349-7006.2011.01943.x
  • Peer Reviewed
• [Journal Article] Stem cells and cancer stem cells-new insights (2011)
  • Author(s): Kondo, T
  • Journal Title: Advances in Cancer Stem Cell Biology Pages: 17-31
  • URL: http://link.springer.com/book/10.1007/978-1-4614-0809-3/page/1
  • Peer Reviewed
• [Journal Article] Stem cells and cancer stem cells-new insights (2011)
  • Author(s): Kondo T.
  • Journal Title: Advances in Cancer Stem Cell Biology Pages: 17-31
• [Journal Article] Tumorigenesis of glioma-initiating cells : Role of Sox11 (2011)
  • Author(s): Kondo T.
  • Journal Title: Stem Cells and Cancer Stem Cells Pages: 93-98
• [Journal Article] Combination of a Ptgs2 inhibitor and an EGFR signaling inhibitor prevents tumorigenesis of oligodendrocyte -lineage derived glioma-initiating cells. (2011)
  • Author(s): Hide, T., Takezaki, T., Nakatani, Y., Nakamura, H., Kuratsu, J., Kondo T
  • Journal Title: Stem Cells Volume: (In press)
  • Peer Reviewed
• [Journal Article] Cytotoxic T lymphocytes efficiently recognize human colon cancer stem-like cells (2011)
  • Author(s): Inoda, S., Hirohashi, Y., Torigoe, T., Morita, R., Takahashi, A., Asanuma H., Nakatsugawa, M., Nishizawa, S., Tamura, Y., Tsuruma, T., Terui, T., Kondo, T., Ishitani, K., Hasegawa, T., Hirata, K., Sato, N.
  • Journal Title: Am.J.Pathol. Volume: 178 Pages: 1805-1813
  • Peer Reviewed
• [Journal Article] Essential role of the Hedgehog signaling pathway in human glioma-initiating cells (2011)
  • Author(s): Takezaki, T., Hide, T., Takanaga, H., Nakamura, H., Kuratsu, J., Kondo T
  • Journal Title: Cancer Science
  • Peer Reviewed
• [Journal Article] 膠芽腫 (2010)
  • Author(s): 近藤亨
  • Journal Title: Clinical Neuroscience Volume: 28 Pages: 24-28
• [Journal Article] 癌幹細胞と分子標的薬 (2010)
  • Author(s): 近藤亨
  • Journal Title: 日本臨床社 Volume: 68 Pages: 986-990
• [Journal Article] 幹細胞生物学からみた脳腫瘍の発生機序 (2010)
  • Author(s): 近藤亨
  • Journal Title: 日本臨床社 Volume: 68 Pages: 24-28
• [Journal Article] 人工グリオーマからの考察 (2010)
  • Author(s): 近藤亨
  • Journal Title: 再生医療 Volume: 9 Pages: 42-46
• [Journal Article] Mouse induced glioma-initiating cell models and therapeutic targets (2010)
  • Author(s): Kondo, T
  • Journal Title: Anticancer Agents Med Chem. Volume: 10 Pages: 471-480
  • Peer Reviewed
• [Journal Article] Mouse induced glioma-initiating cell models and therapeutic targets. (2010)
  • Author(s): Kondo, T.
  • Journal Title: Anticancer agents Med.Chem. Volume: 10 Pages: 471-480
  • Peer Reviewed
• [Presentation] 人工癌幹細胞を用いた基礎研究と治療戦略の創出 (2012)
  • Author(s): 近藤亨
  • Organizer: 第9回日本生化学会北海道支部例会
  • Place of Presentation: 北海道大学
  • Year and Date: 2012-07-20
• [Presentation] 人工癌幹細胞を用いた基礎研究と治療戦略の創出 (2012)
  • Author(s): 近藤 亨
  • Organizer: 第４９回日本生化学会北海道支部例会
  • Place of Presentation: 北海道大学（札幌市）
  • Invited
• [Presentation] グリオーマ幹細胞の分化能力 (2011)
  • Author(s): 近藤亨
  • Organizer: 第70回日本癌学会
  • Place of Presentation: シンポジウ ム、名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] Combination of a Ptgs2 inhibitor and an EGFR-signaling inhibitor prevents tumorigenesis of oligodendrocyte-lineage derived glioma-initiating cells (2011)
  • Author(s): Toru Kondo
  • Organizer: The 9^<th> Stem cell symposium
  • Place of Presentation: Tokyo
  • Year and Date: 2011-09-21
• [Presentation] Sox11 is a novel tumor suppressor in glioma (2011)
  • Author(s): Toru Kondo
  • Organizer: 3rd International Sox Meeting
  • Place of Presentation: Symposium, Grainau (Germany)
  • Year and Date: 2011-09-12
• [Presentation] Combination of a Ptgs2 inhibitor and an EGFR-signaling inhibitor prevents tumorigenesis of oligodendrocyte-lineage derived glioma-initiating cells (2011)
  • Author(s): Toru Kondo
  • Organizer: The 9th Stem cell symposium
  • Place of Presentation: Symposium, Izumi Garden Gallery
  • Year and Date: 2011-05-14
• [Presentation] Sox11 is a novel tumor suppressor in glioma (2011)
  • Author(s): Toru Kondo
  • Organizer: 3rd International Sox Meeting
  • Place of Presentation: Germany
• [Presentation] グリオーマ幹細胞の分化能力 (2011)
  • Author(s): 近藤亨
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋
• [Presentation] Induced glioma-initiating cell models revealnovel therapeutic targets (2010)
  • Author(s): Toru Kondo
  • Organizer: 15th World Congress on Advances in Oncology&13th International Symposium on Molecular Medicine. Symposium
  • Place of Presentation: Hotel Poseidon Resort (Greece)
  • Year and Date: 2010-10-08
• [Presentation] Cancer stem cellと標的分子 (2010)
  • Author(s): 近藤亨
  • Organizer: 第14回日本がん分子標的治療学会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2010-07-08
• [Presentation] 幹細胞の性状解析 (2010)
  • Author(s): 近藤亨
  • Organizer: 第51回日本臨床細胞学会
  • Place of Presentation: シンポジウム、パシフィコ横浜
  • Year and Date: 2010-05-31
• [Presentation] Induced glioma-initiating cell models reveal novel therapeutic targets. (2010)
  • Author(s): Toru Kondo
  • Organizer: 15th World Congress on Advances in Oncology & 13th International Symposium on Molecular Medicine.
  • Place of Presentation: Athens, Greece
• [Presentation] Cancer stem cellと標的分子 (2010)
  • Author(s): 近藤亨
  • Organizer: 第14回日本がん分子標的治療学会
  • Place of Presentation: 東京
• [Presentation] グリオーマ幹細胞の性状解析 (2010)
  • Author(s): 近藤亨
  • Organizer: 第51回日本臨床細胞学会
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://www.igm.hokudai.ac.jp/stemcell/